This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.59 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 73,754 (3.4%) Reads that were too short: 9,040 (0.4%) Reads written (passing filters): 64,714 (3.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 17,456,712 bp (2.6%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 73754 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2817 535.2 0 2817 7 3960 535.2 0 3960 8 26574 535.2 0 26574 9 18161 535.2 0 18161 10 60 535.2 0 60 11 58 535.2 0 58 12 19 535.2 0 19 13 30 535.2 0 30 14 23 535.2 0 23 15 42 535.2 0 42 16 19 535.2 0 19 17 13 535.2 0 13 18 25 535.2 0 25 19 21 535.2 0 21 20 25 535.2 0 25 21 41 535.2 0 41 22 42 535.2 0 42 23 25 535.2 0 25 24 18 535.2 0 18 25 29 535.2 0 29 26 22 535.2 0 22 27 31 535.2 0 31 28 29 535.2 0 29 29 21 535.2 0 21 30 26 535.2 0 26 31 16 535.2 0 16 32 13 535.2 0 13 33 25 535.2 0 25 34 29 535.2 0 29 35 44 535.2 0 44 36 23 535.2 0 23 37 39 535.2 0 39 38 71 535.2 0 71 39 28 535.2 0 28 40 22 535.2 0 22 41 43 535.2 0 43 42 32 535.2 0 32 43 38 535.2 0 38 44 30 535.2 0 30 45 44 535.2 0 44 46 33 535.2 0 33 47 90 535.2 0 90 48 658 535.2 0 658 49 536 535.2 0 536 50 763 535.2 0 763 51 54 535.2 0 54 52 30 535.2 0 30 53 24 535.2 0 24 54 24 535.2 0 24 55 10 535.2 0 10 56 32 535.2 0 32 57 17 535.2 0 17 58 14 535.2 0 14 59 19 535.2 0 19 60 26 535.2 0 26 61 29 535.2 0 29 62 22 535.2 0 22 63 14 535.2 0 14 64 27 535.2 0 27 65 24 535.2 0 24 66 25 535.2 0 25 67 23 535.2 0 23 68 21 535.2 0 21 69 21 535.2 0 21 70 10 535.2 0 10 71 20 535.2 0 20 72 13 535.2 0 13 73 13 535.2 0 13 74 45 535.2 0 45 75 24 535.2 0 24 76 44 535.2 0 44 77 19 535.2 0 19 78 29 535.2 0 29 79 29 535.2 0 29 80 39 535.2 0 39 81 125 535.2 0 125 82 122 535.2 0 122 83 261 535.2 0 261 84 56 535.2 0 56 85 43 535.2 0 43 86 19 535.2 0 19 87 33 535.2 0 33 88 49 535.2 0 49 89 52 535.2 0 52 90 63 535.2 0 63 91 36 535.2 0 36 92 21 535.2 0 21 93 18 535.2 0 18 94 25 535.2 0 25 95 22 535.2 0 22 96 65 535.2 0 65 97 124 535.2 0 124 98 195 535.2 0 195 99 26 535.2 0 26 100 21 535.2 0 21 101 18 535.2 0 18 102 14 535.2 0 14 103 17 535.2 0 17 104 26 535.2 0 26 105 28 535.2 0 28 106 15 535.2 0 15 107 23 535.2 0 23 108 25 535.2 0 25 109 35 535.2 0 35 110 21 535.2 0 21 111 19 535.2 0 19 112 28 535.2 0 28 113 56 535.2 0 56 114 67 535.2 0 67 115 38 535.2 0 38 116 35 535.2 0 35 117 40 535.2 0 40 118 134 535.2 0 134 119 189 535.2 0 189 120 204 535.2 0 204 121 25 535.2 0 25 122 38 535.2 0 38 123 26 535.2 0 26 124 26 535.2 0 26 125 25 535.2 0 25 126 37 535.2 0 37 127 70 535.2 0 70 128 226 535.2 0 226 129 167 535.2 0 167 130 28 535.2 0 28 131 32 535.2 0 32 132 41 535.2 0 41 133 35 535.2 0 35 134 52 535.2 0 52 135 32 535.2 0 32 136 61 535.2 0 61 137 79 535.2 0 79 138 106 535.2 0 106 139 43 535.2 0 43 140 22 535.2 0 22 141 36 535.2 0 36 142 45 535.2 0 45 143 45 535.2 0 45 144 66 535.2 0 66 145 74 535.2 0 74 146 221 535.2 0 221 147 96 535.2 0 96 148 377 535.2 0 377 149 29 535.2 0 29 150 24 535.2 0 24 151 56 535.2 0 56 152 30 535.2 0 30 153 77 535.2 0 77 154 41 535.2 0 41 155 79 535.2 0 79 156 26 535.2 0 26 157 39 535.2 0 39 158 54 535.2 0 54 159 23 535.2 0 23 160 17 535.2 0 17 161 21 535.2 0 21 162 26 535.2 0 26 163 22 535.2 0 22 164 24 535.2 0 24 165 25 535.2 0 25 166 26 535.2 0 26 167 20 535.2 0 20 168 41 535.2 0 41 169 20 535.2 0 20 170 48 535.2 0 48 171 34 535.2 0 34 172 39 535.2 0 39 173 27 535.2 0 27 174 9 535.2 0 9 175 17 535.2 0 17 176 37 535.2 0 37 177 28 535.2 0 28 178 52 535.2 0 52 179 39 535.2 0 39 180 37 535.2 0 37 181 46 535.2 0 46 182 97 535.2 0 97 183 45 535.2 0 45 184 26 535.2 0 26 185 25 535.2 0 25 186 27 535.2 0 27 187 31 535.2 0 31 188 34 535.2 0 34 189 57 535.2 0 57 190 72 535.2 0 72 191 80 535.2 0 80 192 70 535.2 0 70 193 99 535.2 0 99 194 183 535.2 0 183 195 879 535.2 0 879 196 857 535.2 0 857 197 871 535.2 0 871 198 51 535.2 0 51 199 27 535.2 0 27 200 27 535.2 0 27 201 35 535.2 0 35 202 48 535.2 0 48 203 34 535.2 0 34 204 36 535.2 0 36 205 16 535.2 0 16 206 31 535.2 0 31 207 26 535.2 0 26 208 38 535.2 0 38 209 43 535.2 0 43 210 35 535.2 0 35 211 30 535.2 0 30 212 51 535.2 0 51 213 72 535.2 0 72 214 146 535.2 0 146 215 163 535.2 0 163 216 116 535.2 0 116 217 37 535.2 0 37 218 51 535.2 0 51 219 40 535.2 0 40 220 66 535.2 0 66 221 63 535.2 0 63 222 40 535.2 0 40 223 49 535.2 0 49 224 47 535.2 0 47 225 46 535.2 0 46 226 41 535.2 0 41 227 32 535.2 0 32 228 27 535.2 0 27 229 38 535.2 0 38 230 58 535.2 0 58 231 76 535.2 0 76 232 45 535.2 0 45 233 73 535.2 0 73 234 90 535.2 0 90 235 85 535.2 0 85 236 126 535.2 0 126 237 58 535.2 0 58 238 57 535.2 0 57 239 125 535.2 0 125 240 144 535.2 0 144 241 115 535.2 0 115 242 77 535.2 0 77 243 280 535.2 0 280 244 232 535.2 0 232 245 326 535.2 0 326 246 88 535.2 0 88 247 56 535.2 0 56 248 53 535.2 0 53 249 95 535.2 0 95 250 139 535.2 0 139 251 158 535.2 0 158 252 145 535.2 0 145 253 111 535.2 0 111 254 127 535.2 0 127 255 100 535.2 0 100 256 81 535.2 0 81 257 74 535.2 0 74 258 81 535.2 0 81 259 75 535.2 0 75 260 118 535.2 0 118 261 221 535.2 0 221 262 167 535.2 0 167 263 152 535.2 0 152 264 85 535.2 0 85 265 74 535.2 0 74 266 64 535.2 0 64 267 35 535.2 0 35 268 46 535.2 0 46 269 105 535.2 0 105 270 158 535.2 0 158 271 114 535.2 0 114 272 46 535.2 0 46 273 60 535.2 0 60 274 42 535.2 0 42 275 62 535.2 0 62 276 46 535.2 0 46 277 49 535.2 0 49 278 41 535.2 0 41 279 37 535.2 0 37 280 50 535.2 0 50 281 65 535.2 0 65 282 70 535.2 0 70 283 53 535.2 0 53 284 93 535.2 0 93 285 56 535.2 0 56 286 98 535.2 0 98 287 125 535.2 0 125 288 140 535.2 0 140 289 211 535.2 0 211 290 157 535.2 0 157 291 160 535.2 0 160 292 94 535.2 0 94 293 118 535.2 0 118 294 80 535.2 0 80 295 82 535.2 0 82 296 100 535.2 0 100 297 203 535.2 0 203 298 178 535.2 0 178 299 230 535.2 0 230 300 107 535.2 0 107 301 36 535.2 0 36 Finished in 3.42 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 64,714 Reads with adapters: 23,987 (37.1%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 23,962 (37.0%) Total basepairs processed: 17,456,712 bp Total written (filtered): 6,514,469 bp (37.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23987 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 7 2 18 21 0.0 3 0 0 11 5 5 19 361 0.0 3 0 282 38 29 12 20 19885 0.0 4 18121 1349 296 91 28 21 120 0.0 4 11 88 11 4 6 22 3319 0.0 4 3002 249 50 13 5 23 156 0.0 4 3 12 1 86 54 24 4 0.0 4 0 0 2 0 2 25 26 0.0 4 0 0 0 16 10 27 2 0.0 4 0 0 0 1 1 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 1 39 1 0.0 4 1 41 1 0.0 4 0 0 0 1 60 1 0.0 4 1 84 1 0.0 4 0 0 0 0 1 86 4 0.0 4 0 0 0 0 4 94 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 39 0.0 4 0 0 0 0 39 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 1 153 4 0.0 4 0 0 0 0 4 161 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 1 1 227 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 1 2 257 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 284 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 23,962 Reads with adapters: 5,682 (23.7%) Reads written (passing filters): 23,962 (100.0%) Total basepairs processed: 6,514,469 bp Total written (filtered): 6,448,382 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5682 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 10.8% G: 83.8% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 20 374.4 0 20 4 6 93.6 0 6 6 2 5.9 0 2 7 1 1.5 0 1 8 1 0.4 0 1 9 931 0.1 0 97 834 10 3697 0.0 1 307 3390 11 915 0.0 1 42 873 12 18 0.0 1 0 18 13 27 0.0 1 0 27 14 1 0.0 1 0 1 15 1 0.0 1 0 1 78 1 0.0 1 0 1 150 39 0.0 1 32 7 151 5 0.0 1 3 2 152 2 0.0 1 1 1 153 2 0.0 1 2 177 1 0.0 1 0 1 189 1 0.0 1 0 1 208 7 0.0 1 6 1 209 2 0.0 1 2 210 1 0.0 1 1 223 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.