This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.67 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 64,478 (2.9%) Reads that were too short: 3,528 (0.2%) Reads written (passing filters): 60,950 (2.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 17,183,207 bp (2.6%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 64478 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2800 535.2 0 2800 7 4552 535.2 0 4552 8 23305 535.2 0 23305 9 24604 535.2 0 24604 10 41 535.2 0 41 11 22 535.2 0 22 12 12 535.2 0 12 13 10 535.2 0 10 14 15 535.2 0 15 15 17 535.2 0 17 16 12 535.2 0 12 17 9 535.2 0 9 18 15 535.2 0 15 19 8 535.2 0 8 20 9 535.2 0 9 21 11 535.2 0 11 22 23 535.2 0 23 23 16 535.2 0 16 24 11 535.2 0 11 25 11 535.2 0 11 26 14 535.2 0 14 27 16 535.2 0 16 28 12 535.2 0 12 29 5 535.2 0 5 30 24 535.2 0 24 31 13 535.2 0 13 32 12 535.2 0 12 33 12 535.2 0 12 34 19 535.2 0 19 35 17 535.2 0 17 36 21 535.2 0 21 37 21 535.2 0 21 38 21 535.2 0 21 39 18 535.2 0 18 40 13 535.2 0 13 41 15 535.2 0 15 42 15 535.2 0 15 43 20 535.2 0 20 44 7 535.2 0 7 45 22 535.2 0 22 46 24 535.2 0 24 47 18 535.2 0 18 48 126 535.2 0 126 49 181 535.2 0 181 50 233 535.2 0 233 51 9 535.2 0 9 52 9 535.2 0 9 53 9 535.2 0 9 54 9 535.2 0 9 55 16 535.2 0 16 56 16 535.2 0 16 57 9 535.2 0 9 58 8 535.2 0 8 59 17 535.2 0 17 60 8 535.2 0 8 61 19 535.2 0 19 62 11 535.2 0 11 63 9 535.2 0 9 64 12 535.2 0 12 65 14 535.2 0 14 66 12 535.2 0 12 67 15 535.2 0 15 68 15 535.2 0 15 69 11 535.2 0 11 70 8 535.2 0 8 71 22 535.2 0 22 72 9 535.2 0 9 73 11 535.2 0 11 74 14 535.2 0 14 75 9 535.2 0 9 76 8 535.2 0 8 77 9 535.2 0 9 78 13 535.2 0 13 79 13 535.2 0 13 80 18 535.2 0 18 81 24 535.2 0 24 82 26 535.2 0 26 83 55 535.2 0 55 84 18 535.2 0 18 85 41 535.2 0 41 86 15 535.2 0 15 87 11 535.2 0 11 88 24 535.2 0 24 89 22 535.2 0 22 90 49 535.2 0 49 91 14 535.2 0 14 92 14 535.2 0 14 93 15 535.2 0 15 94 16 535.2 0 16 95 15 535.2 0 15 96 22 535.2 0 22 97 27 535.2 0 27 98 37 535.2 0 37 99 21 535.2 0 21 100 12 535.2 0 12 101 8 535.2 0 8 102 3 535.2 0 3 103 8 535.2 0 8 104 17 535.2 0 17 105 11 535.2 0 11 106 9 535.2 0 9 107 11 535.2 0 11 108 10 535.2 0 10 109 16 535.2 0 16 110 14 535.2 0 14 111 7 535.2 0 7 112 13 535.2 0 13 113 20 535.2 0 20 114 26 535.2 0 26 115 17 535.2 0 17 116 20 535.2 0 20 117 12 535.2 0 12 118 42 535.2 0 42 119 43 535.2 0 43 120 62 535.2 0 62 121 10 535.2 0 10 122 23 535.2 0 23 123 12 535.2 0 12 124 17 535.2 0 17 125 12 535.2 0 12 126 11 535.2 0 11 127 28 535.2 0 28 128 73 535.2 0 73 129 46 535.2 0 46 130 17 535.2 0 17 131 13 535.2 0 13 132 22 535.2 0 22 133 22 535.2 0 22 134 18 535.2 0 18 135 28 535.2 0 28 136 24 535.2 0 24 137 36 535.2 0 36 138 43 535.2 0 43 139 24 535.2 0 24 140 20 535.2 0 20 141 22 535.2 0 22 142 15 535.2 0 15 143 19 535.2 0 19 144 21 535.2 0 21 145 40 535.2 0 40 146 257 535.2 0 257 147 94 535.2 0 94 148 395 535.2 0 395 149 22 535.2 0 22 150 17 535.2 0 17 151 11 535.2 0 11 152 14 535.2 0 14 153 86 535.2 0 86 154 30 535.2 0 30 155 47 535.2 0 47 156 16 535.2 0 16 157 13 535.2 0 13 158 13 535.2 0 13 159 14 535.2 0 14 160 19 535.2 0 19 161 16 535.2 0 16 162 15 535.2 0 15 163 15 535.2 0 15 164 15 535.2 0 15 165 19 535.2 0 19 166 19 535.2 0 19 167 23 535.2 0 23 168 18 535.2 0 18 169 24 535.2 0 24 170 24 535.2 0 24 171 21 535.2 0 21 172 17 535.2 0 17 173 13 535.2 0 13 174 6 535.2 0 6 175 19 535.2 0 19 176 24 535.2 0 24 177 29 535.2 0 29 178 21 535.2 0 21 179 31 535.2 0 31 180 14 535.2 0 14 181 40 535.2 0 40 182 76 535.2 0 76 183 41 535.2 0 41 184 9 535.2 0 9 185 20 535.2 0 20 186 13 535.2 0 13 187 17 535.2 0 17 188 29 535.2 0 29 189 25 535.2 0 25 190 30 535.2 0 30 191 36 535.2 0 36 192 42 535.2 0 42 193 48 535.2 0 48 194 45 535.2 0 45 195 274 535.2 0 274 196 228 535.2 0 228 197 255 535.2 0 255 198 23 535.2 0 23 199 8 535.2 0 8 200 19 535.2 0 19 201 14 535.2 0 14 202 16 535.2 0 16 203 20 535.2 0 20 204 17 535.2 0 17 205 14 535.2 0 14 206 10 535.2 0 10 207 16 535.2 0 16 208 12 535.2 0 12 209 18 535.2 0 18 210 16 535.2 0 16 211 16 535.2 0 16 212 20 535.2 0 20 213 19 535.2 0 19 214 29 535.2 0 29 215 38 535.2 0 38 216 41 535.2 0 41 217 13 535.2 0 13 218 25 535.2 0 25 219 20 535.2 0 20 220 15 535.2 0 15 221 34 535.2 0 34 222 18 535.2 0 18 223 18 535.2 0 18 224 10 535.2 0 10 225 18 535.2 0 18 226 15 535.2 0 15 227 10 535.2 0 10 228 11 535.2 0 11 229 11 535.2 0 11 230 15 535.2 0 15 231 24 535.2 0 24 232 23 535.2 0 23 233 20 535.2 0 20 234 27 535.2 0 27 235 25 535.2 0 25 236 120 535.2 0 120 237 24 535.2 0 24 238 21 535.2 0 21 239 35 535.2 0 35 240 31 535.2 0 31 241 29 535.2 0 29 242 22 535.2 0 22 243 96 535.2 0 96 244 59 535.2 0 59 245 80 535.2 0 80 246 26 535.2 0 26 247 20 535.2 0 20 248 19 535.2 0 19 249 24 535.2 0 24 250 33 535.2 0 33 251 114 535.2 0 114 252 51 535.2 0 51 253 40 535.2 0 40 254 27 535.2 0 27 255 83 535.2 0 83 256 29 535.2 0 29 257 19 535.2 0 19 258 26 535.2 0 26 259 35 535.2 0 35 260 34 535.2 0 34 261 75 535.2 0 75 262 50 535.2 0 50 263 44 535.2 0 44 264 22 535.2 0 22 265 27 535.2 0 27 266 19 535.2 0 19 267 20 535.2 0 20 268 22 535.2 0 22 269 29 535.2 0 29 270 32 535.2 0 32 271 33 535.2 0 33 272 23 535.2 0 23 273 18 535.2 0 18 274 19 535.2 0 19 275 30 535.2 0 30 276 27 535.2 0 27 277 15 535.2 0 15 278 15 535.2 0 15 279 18 535.2 0 18 280 12 535.2 0 12 281 14 535.2 0 14 282 20 535.2 0 20 283 19 535.2 0 19 284 35 535.2 0 35 285 20 535.2 0 20 286 43 535.2 0 43 287 114 535.2 0 114 288 128 535.2 0 128 289 106 535.2 0 106 290 82 535.2 0 82 291 80 535.2 0 80 292 47 535.2 0 47 293 35 535.2 0 35 294 35 535.2 0 35 295 28 535.2 0 28 296 38 535.2 0 38 297 74 535.2 0 74 298 68 535.2 0 68 299 84 535.2 0 84 300 128 535.2 0 128 301 29 535.2 0 29 Finished in 2.98 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 60,950 Reads with adapters: 24,474 (40.2%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 24,462 (40.1%) Total basepairs processed: 17,183,207 bp Total written (filtered): 6,747,621 bp (39.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24474 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 7 0.0 3 0 0 7 16 160 0.0 3 0 153 7 17 21977 0.0 3 21600 359 14 4 18 83 0.0 3 2 72 9 19 2164 0.0 3 2117 34 13 20 38 0.0 3 0 7 1 30 21 1 0.0 3 0 0 1 22 1 0.0 3 0 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 45 6 0.0 3 0 0 0 6 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 110 1 0.0 3 0 1 114 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 1 244 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.86 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 24,462 Reads with adapters: 38 (0.2%) Reads written (passing filters): 24,462 (100.0%) Total basepairs processed: 6,747,621 bp Total written (filtered): 6,747,486 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 38 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.4% C: 5.3% G: 18.4% T: 7.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 18 382.2 0 18 4 19 95.6 0 19 5 1 23.9 0 1