This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.35 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 79,380 (3.6%) Reads that were too short: 28,254 (1.3%) Reads written (passing filters): 51,126 (2.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 11,671,177 bp (1.8%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 79380 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1226 535.2 0 1226 7 8464 535.2 0 8464 8 7374 535.2 0 7374 9 130 535.2 0 130 10 580 535.2 0 580 11 55 535.2 0 55 12 52 535.2 0 52 13 59 535.2 0 59 14 74 535.2 0 74 15 62 535.2 0 62 16 62 535.2 0 62 17 86 535.2 0 86 18 99 535.2 0 99 19 144 535.2 0 144 20 56 535.2 0 56 21 57 535.2 0 57 22 32 535.2 0 32 23 53 535.2 0 53 24 49 535.2 0 49 25 64 535.2 0 64 26 66 535.2 0 66 27 68 535.2 0 68 28 65 535.2 0 65 29 90 535.2 0 90 30 76 535.2 0 76 31 49 535.2 0 49 32 58 535.2 0 58 33 74 535.2 0 74 34 53 535.2 0 53 35 71 535.2 0 71 36 65 535.2 0 65 37 56 535.2 0 56 38 47 535.2 0 47 39 62 535.2 0 62 40 81 535.2 0 81 41 37 535.2 0 37 42 76 535.2 0 76 43 85 535.2 0 85 44 42 535.2 0 42 45 47 535.2 0 47 46 76 535.2 0 76 47 93 535.2 0 93 48 99 535.2 0 99 49 149 535.2 0 149 50 84 535.2 0 84 51 66 535.2 0 66 52 120 535.2 0 120 53 193 535.2 0 193 54 185 535.2 0 185 55 87 535.2 0 87 56 137 535.2 0 137 57 130 535.2 0 130 58 112 535.2 0 112 59 73 535.2 0 73 60 157 535.2 0 157 61 182 535.2 0 182 62 262 535.2 0 262 63 63 535.2 0 63 64 115 535.2 0 115 65 74 535.2 0 74 66 83 535.2 0 83 67 116 535.2 0 116 68 83 535.2 0 83 69 74 535.2 0 74 70 132 535.2 0 132 71 164 535.2 0 164 72 174 535.2 0 174 73 634 535.2 0 634 74 1067 535.2 0 1067 75 1057 535.2 0 1057 76 204 535.2 0 204 77 2564 535.2 0 2564 78 3772 535.2 0 3772 79 1826 535.2 0 1826 80 57 535.2 0 57 81 87 535.2 0 87 82 79 535.2 0 79 83 71 535.2 0 71 84 80 535.2 0 80 85 50 535.2 0 50 86 102 535.2 0 102 87 69 535.2 0 69 88 76 535.2 0 76 89 87 535.2 0 87 90 79 535.2 0 79 91 68 535.2 0 68 92 91 535.2 0 91 93 74 535.2 0 74 94 81 535.2 0 81 95 67 535.2 0 67 96 86 535.2 0 86 97 97 535.2 0 97 98 66 535.2 0 66 99 76 535.2 0 76 100 67 535.2 0 67 101 71 535.2 0 71 102 63 535.2 0 63 103 62 535.2 0 62 104 73 535.2 0 73 105 75 535.2 0 75 106 268 535.2 0 268 107 163 535.2 0 163 108 191 535.2 0 191 109 77 535.2 0 77 110 57 535.2 0 57 111 69 535.2 0 69 112 61 535.2 0 61 113 76 535.2 0 76 114 164 535.2 0 164 115 113 535.2 0 113 116 157 535.2 0 157 117 78 535.2 0 78 118 77 535.2 0 77 119 52 535.2 0 52 120 78 535.2 0 78 121 67 535.2 0 67 122 77 535.2 0 77 123 61 535.2 0 61 124 71 535.2 0 71 125 85 535.2 0 85 126 100 535.2 0 100 127 227 535.2 0 227 128 270 535.2 0 270 129 405 535.2 0 405 130 358 535.2 0 358 131 99 535.2 0 99 132 82 535.2 0 82 133 125 535.2 0 125 134 185 535.2 0 185 135 304 535.2 0 304 136 286 535.2 0 286 137 138 535.2 0 138 138 88 535.2 0 88 139 113 535.2 0 113 140 116 535.2 0 116 141 70 535.2 0 70 142 60 535.2 0 60 143 69 535.2 0 69 144 75 535.2 0 75 145 143 535.2 0 143 146 249 535.2 0 249 147 635 535.2 0 635 148 494 535.2 0 494 149 99 535.2 0 99 150 75 535.2 0 75 151 56 535.2 0 56 152 59 535.2 0 59 153 82 535.2 0 82 154 106 535.2 0 106 155 471 535.2 0 471 156 140 535.2 0 140 157 143 535.2 0 143 158 89 535.2 0 89 159 139 535.2 0 139 160 246 535.2 0 246 161 208 535.2 0 208 162 79 535.2 0 79 163 92 535.2 0 92 164 186 535.2 0 186 165 286 535.2 0 286 166 304 535.2 0 304 167 123 535.2 0 123 168 99 535.2 0 99 169 70 535.2 0 70 170 86 535.2 0 86 171 81 535.2 0 81 172 81 535.2 0 81 173 66 535.2 0 66 174 78 535.2 0 78 175 83 535.2 0 83 176 50 535.2 0 50 177 55 535.2 0 55 178 86 535.2 0 86 179 121 535.2 0 121 180 109 535.2 0 109 181 89 535.2 0 89 182 84 535.2 0 84 183 91 535.2 0 91 184 248 535.2 0 248 185 361 535.2 0 361 186 520 535.2 0 520 187 171 535.2 0 171 188 87 535.2 0 87 189 82 535.2 0 82 190 85 535.2 0 85 191 77 535.2 0 77 192 116 535.2 0 116 193 95 535.2 0 95 194 95 535.2 0 95 195 331 535.2 0 331 196 500 535.2 0 500 197 456 535.2 0 456 198 174 535.2 0 174 199 109 535.2 0 109 200 86 535.2 0 86 201 81 535.2 0 81 202 76 535.2 0 76 203 85 535.2 0 85 204 94 535.2 0 94 205 66 535.2 0 66 206 87 535.2 0 87 207 94 535.2 0 94 208 103 535.2 0 103 209 93 535.2 0 93 210 141 535.2 0 141 211 108 535.2 0 108 212 156 535.2 0 156 213 132 535.2 0 132 214 125 535.2 0 125 215 321 535.2 0 321 216 467 535.2 0 467 217 391 535.2 0 391 218 109 535.2 0 109 219 134 535.2 0 134 220 128 535.2 0 128 221 118 535.2 0 118 222 83 535.2 0 83 223 85 535.2 0 85 224 86 535.2 0 86 225 200 535.2 0 200 226 244 535.2 0 244 227 153 535.2 0 153 228 76 535.2 0 76 229 77 535.2 0 77 230 111 535.2 0 111 231 118 535.2 0 118 232 112 535.2 0 112 233 128 535.2 0 128 234 201 535.2 0 201 235 373 535.2 0 373 236 2481 535.2 0 2481 237 2490 535.2 0 2490 238 2331 535.2 0 2331 239 129 535.2 0 129 240 134 535.2 0 134 241 101 535.2 0 101 242 115 535.2 0 115 243 93 535.2 0 93 244 103 535.2 0 103 245 192 535.2 0 192 246 348 535.2 0 348 247 608 535.2 0 608 248 522 535.2 0 522 249 224 535.2 0 224 250 185 535.2 0 185 251 220 535.2 0 220 252 274 535.2 0 274 253 358 535.2 0 358 254 321 535.2 0 321 255 326 535.2 0 326 256 121 535.2 0 121 257 113 535.2 0 113 258 108 535.2 0 108 259 102 535.2 0 102 260 159 535.2 0 159 261 95 535.2 0 95 262 94 535.2 0 94 263 144 535.2 0 144 264 109 535.2 0 109 265 117 535.2 0 117 266 95 535.2 0 95 267 86 535.2 0 86 268 129 535.2 0 129 269 99 535.2 0 99 270 104 535.2 0 104 271 95 535.2 0 95 272 127 535.2 0 127 273 161 535.2 0 161 274 216 535.2 0 216 275 146 535.2 0 146 276 92 535.2 0 92 277 110 535.2 0 110 278 198 535.2 0 198 279 146 535.2 0 146 280 213 535.2 0 213 281 233 535.2 0 233 282 336 535.2 0 336 283 286 535.2 0 286 284 376 535.2 0 376 285 455 535.2 0 455 286 493 535.2 0 493 287 180 535.2 0 180 288 126 535.2 0 126 289 164 535.2 0 164 290 147 535.2 0 147 291 171 535.2 0 171 292 141 535.2 0 141 293 237 535.2 0 237 294 304 535.2 0 304 295 432 535.2 0 432 296 917 535.2 0 917 297 974 535.2 0 974 298 962 535.2 0 962 299 432 535.2 0 432 300 366 535.2 0 366 301 713 535.2 0 713 Finished in 2.45 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 51,126 Reads with adapters: 8,289 (16.2%) Reads that were too short: 40 (0.1%) Reads written (passing filters): 8,249 (16.1%) Total basepairs processed: 11,671,177 bp Total written (filtered): 2,244,513 bp (19.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8289 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 7 0.0 3 0 0 4 1 2 19 141 0.0 3 0 104 19 13 5 20 7299 0.0 4 6525 574 149 37 14 21 36 0.0 4 6 17 6 4 3 22 650 0.0 4 581 53 12 3 1 23 43 0.0 4 1 3 0 23 16 24 1 0.0 4 0 0 0 0 1 25 3 0.0 4 0 0 1 2 26 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 39 2 0.0 4 0 0 0 0 2 57 39 0.0 4 0 0 0 0 39 58 1 0.0 4 0 0 0 0 1 59 2 0.0 4 0 0 0 0 2 60 1 0.0 4 0 0 0 0 1 61 7 0.0 4 0 0 0 0 7 68 1 0.0 4 0 0 0 1 74 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 2 0.0 4 0 0 0 0 2 83 2 0.0 4 0 0 0 0 2 87 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 100 3 0.0 4 0 0 0 0 3 102 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 127 5 0.0 4 0 0 0 0 5 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 154 4 0.0 4 0 0 0 0 4 163 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 1 189 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 215 3 0.0 4 0 0 0 0 3 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 8,249 Reads with adapters: 1,804 (21.9%) Reads written (passing filters): 8,249 (100.0%) Total basepairs processed: 2,244,513 bp Total written (filtered): 2,224,398 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1804 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.1% C: 10.8% G: 84.3% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 24 128.9 0 24 4 11 32.2 0 11 6 1 2.0 0 1 7 3 0.5 0 3 8 1 0.1 0 1 9 76 0.0 0 7 69 10 162 0.0 1 12 150 11 1501 0.0 1 68 1433 12 14 0.0 1 0 14 13 1 0.0 1 0 1 14 5 0.0 1 0 5 170 1 0.0 1 1 173 3 0.0 1 2 1 209 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.