This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.48 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 58,499 (2.7%) Reads that were too short: 19,686 (0.9%) Reads written (passing filters): 38,813 (1.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 9,329,609 bp (1.4%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 58499 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1283 535.2 0 1283 7 7704 535.2 0 7704 8 9169 535.2 0 9169 9 59 535.2 0 59 10 171 535.2 0 171 11 55 535.2 0 55 12 69 535.2 0 69 13 59 535.2 0 59 14 58 535.2 0 58 15 71 535.2 0 71 16 61 535.2 0 61 17 55 535.2 0 55 18 47 535.2 0 47 19 56 535.2 0 56 20 49 535.2 0 49 21 50 535.2 0 50 22 51 535.2 0 51 23 57 535.2 0 57 24 41 535.2 0 41 25 55 535.2 0 55 26 65 535.2 0 65 27 45 535.2 0 45 28 50 535.2 0 50 29 71 535.2 0 71 30 77 535.2 0 77 31 38 535.2 0 38 32 52 535.2 0 52 33 77 535.2 0 77 34 50 535.2 0 50 35 58 535.2 0 58 36 52 535.2 0 52 37 48 535.2 0 48 38 53 535.2 0 53 39 65 535.2 0 65 40 66 535.2 0 66 41 49 535.2 0 49 42 75 535.2 0 75 43 72 535.2 0 72 44 47 535.2 0 47 45 58 535.2 0 58 46 102 535.2 0 102 47 74 535.2 0 74 48 52 535.2 0 52 49 75 535.2 0 75 50 58 535.2 0 58 51 53 535.2 0 53 52 76 535.2 0 76 53 104 535.2 0 104 54 69 535.2 0 69 55 83 535.2 0 83 56 122 535.2 0 122 57 93 535.2 0 93 58 86 535.2 0 86 59 72 535.2 0 72 60 105 535.2 0 105 61 106 535.2 0 106 62 285 535.2 0 285 63 217 535.2 0 217 64 80 535.2 0 80 65 50 535.2 0 50 66 78 535.2 0 78 67 71 535.2 0 71 68 55 535.2 0 55 69 60 535.2 0 60 70 86 535.2 0 86 71 93 535.2 0 93 72 79 535.2 0 79 73 350 535.2 0 350 74 606 535.2 0 606 75 490 535.2 0 490 76 96 535.2 0 96 77 606 535.2 0 606 78 906 535.2 0 906 79 466 535.2 0 466 80 52 535.2 0 52 81 108 535.2 0 108 82 85 535.2 0 85 83 37 535.2 0 37 84 47 535.2 0 47 85 62 535.2 0 62 86 73 535.2 0 73 87 46 535.2 0 46 88 71 535.2 0 71 89 71 535.2 0 71 90 60 535.2 0 60 91 60 535.2 0 60 92 58 535.2 0 58 93 63 535.2 0 63 94 50 535.2 0 50 95 42 535.2 0 42 96 64 535.2 0 64 97 82 535.2 0 82 98 72 535.2 0 72 99 80 535.2 0 80 100 70 535.2 0 70 101 77 535.2 0 77 102 55 535.2 0 55 103 59 535.2 0 59 104 68 535.2 0 68 105 85 535.2 0 85 106 283 535.2 0 283 107 214 535.2 0 214 108 215 535.2 0 215 109 94 535.2 0 94 110 70 535.2 0 70 111 64 535.2 0 64 112 91 535.2 0 91 113 85 535.2 0 85 114 108 535.2 0 108 115 87 535.2 0 87 116 166 535.2 0 166 117 93 535.2 0 93 118 82 535.2 0 82 119 58 535.2 0 58 120 84 535.2 0 84 121 75 535.2 0 75 122 58 535.2 0 58 123 75 535.2 0 75 124 78 535.2 0 78 125 76 535.2 0 76 126 68 535.2 0 68 127 231 535.2 0 231 128 243 535.2 0 243 129 397 535.2 0 397 130 380 535.2 0 380 131 73 535.2 0 73 132 57 535.2 0 57 133 90 535.2 0 90 134 134 535.2 0 134 135 266 535.2 0 266 136 195 535.2 0 195 137 106 535.2 0 106 138 62 535.2 0 62 139 76 535.2 0 76 140 84 535.2 0 84 141 68 535.2 0 68 142 63 535.2 0 63 143 62 535.2 0 62 144 70 535.2 0 70 145 113 535.2 0 113 146 214 535.2 0 214 147 579 535.2 0 579 148 417 535.2 0 417 149 90 535.2 0 90 150 66 535.2 0 66 151 60 535.2 0 60 152 61 535.2 0 61 153 68 535.2 0 68 154 95 535.2 0 95 155 478 535.2 0 478 156 146 535.2 0 146 157 146 535.2 0 146 158 75 535.2 0 75 159 53 535.2 0 53 160 78 535.2 0 78 161 94 535.2 0 94 162 63 535.2 0 63 163 56 535.2 0 56 164 87 535.2 0 87 165 113 535.2 0 113 166 132 535.2 0 132 167 78 535.2 0 78 168 42 535.2 0 42 169 64 535.2 0 64 170 71 535.2 0 71 171 48 535.2 0 48 172 50 535.2 0 50 173 33 535.2 0 33 174 56 535.2 0 56 175 40 535.2 0 40 176 34 535.2 0 34 177 60 535.2 0 60 178 61 535.2 0 61 179 49 535.2 0 49 180 44 535.2 0 44 181 53 535.2 0 53 182 41 535.2 0 41 183 50 535.2 0 50 184 94 535.2 0 94 185 148 535.2 0 148 186 166 535.2 0 166 187 77 535.2 0 77 188 50 535.2 0 50 189 51 535.2 0 51 190 51 535.2 0 51 191 57 535.2 0 57 192 70 535.2 0 70 193 72 535.2 0 72 194 60 535.2 0 60 195 195 535.2 0 195 196 196 535.2 0 196 197 153 535.2 0 153 198 73 535.2 0 73 199 71 535.2 0 71 200 49 535.2 0 49 201 52 535.2 0 52 202 43 535.2 0 43 203 52 535.2 0 52 204 75 535.2 0 75 205 51 535.2 0 51 206 65 535.2 0 65 207 46 535.2 0 46 208 53 535.2 0 53 209 73 535.2 0 73 210 71 535.2 0 71 211 52 535.2 0 52 212 67 535.2 0 67 213 67 535.2 0 67 214 68 535.2 0 68 215 137 535.2 0 137 216 263 535.2 0 263 217 175 535.2 0 175 218 69 535.2 0 69 219 64 535.2 0 64 220 70 535.2 0 70 221 51 535.2 0 51 222 63 535.2 0 63 223 90 535.2 0 90 224 56 535.2 0 56 225 101 535.2 0 101 226 169 535.2 0 169 227 71 535.2 0 71 228 54 535.2 0 54 229 77 535.2 0 77 230 72 535.2 0 72 231 111 535.2 0 111 232 77 535.2 0 77 233 114 535.2 0 114 234 187 535.2 0 187 235 345 535.2 0 345 236 3362 535.2 0 3362 237 3418 535.2 0 3418 238 3158 535.2 0 3158 239 80 535.2 0 80 240 70 535.2 0 70 241 69 535.2 0 69 242 61 535.2 0 61 243 45 535.2 0 45 244 62 535.2 0 62 245 91 535.2 0 91 246 159 535.2 0 159 247 291 535.2 0 291 248 256 535.2 0 256 249 106 535.2 0 106 250 63 535.2 0 63 251 63 535.2 0 63 252 84 535.2 0 84 253 85 535.2 0 85 254 81 535.2 0 81 255 78 535.2 0 78 256 59 535.2 0 59 257 53 535.2 0 53 258 41 535.2 0 41 259 56 535.2 0 56 260 183 535.2 0 183 261 48 535.2 0 48 262 47 535.2 0 47 263 63 535.2 0 63 264 59 535.2 0 59 265 63 535.2 0 63 266 54 535.2 0 54 267 55 535.2 0 55 268 50 535.2 0 50 269 56 535.2 0 56 270 55 535.2 0 55 271 36 535.2 0 36 272 91 535.2 0 91 273 65 535.2 0 65 274 91 535.2 0 91 275 43 535.2 0 43 276 62 535.2 0 62 277 78 535.2 0 78 278 102 535.2 0 102 279 86 535.2 0 86 280 91 535.2 0 91 281 112 535.2 0 112 282 191 535.2 0 191 283 159 535.2 0 159 284 132 535.2 0 132 285 149 535.2 0 149 286 116 535.2 0 116 287 66 535.2 0 66 288 60 535.2 0 60 289 98 535.2 0 98 290 55 535.2 0 55 291 80 535.2 0 80 292 67 535.2 0 67 293 95 535.2 0 95 294 111 535.2 0 111 295 123 535.2 0 123 296 321 535.2 0 321 297 326 535.2 0 326 298 287 535.2 0 287 299 146 535.2 0 146 300 127 535.2 0 127 301 297 535.2 0 297 Finished in 1.76 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 38,813 Reads with adapters: 7,856 (20.2%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 7,837 (20.2%) Total basepairs processed: 9,329,609 bp Total written (filtered): 2,156,217 bp (23.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7856 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 65 0.0 3 0 63 2 17 7630 0.0 3 7455 169 5 1 18 29 0.0 3 2 26 1 19 4 0.0 3 1 0 3 20 6 0.0 3 0 0 1 5 27 1 0.0 3 1 39 28 0.0 3 0 0 0 28 43 1 0.0 3 0 0 0 1 45 10 0.0 3 0 0 0 10 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 64 5 0.0 3 0 0 0 5 70 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 94 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 20 0.0 3 0 0 0 20 115 6 0.0 3 0 0 0 6 117 2 0.0 3 0 0 0 2 118 7 0.0 3 0 0 0 7 124 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 180 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 7,837 Reads with adapters: 8 (0.1%) Reads written (passing filters): 7,837 (100.0%) Total basepairs processed: 2,156,217 bp Total written (filtered): 2,156,181 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 8 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 25.0% G: 25.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 122.5 0 5 4 2 30.6 0 2 13 1 0.0 1 0 1