This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.67 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 107,716 (4.9%) Reads that were too short: 31,428 (1.4%) Reads written (passing filters): 76,288 (3.5%) Total basepairs processed: 659,880,795 bp Total written (filtered): 20,303,311 bp (3.1%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 107716 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 876 535.2 0 876 7 26812 535.2 0 26812 8 609 535.2 0 609 9 31700 535.2 0 31700 10 51 535.2 0 51 11 15 535.2 0 15 12 22 535.2 0 22 13 17 535.2 0 17 14 27 535.2 0 27 15 15 535.2 0 15 16 21 535.2 0 21 17 23 535.2 0 23 18 11 535.2 0 11 19 24 535.2 0 24 20 11 535.2 0 11 21 13 535.2 0 13 22 11 535.2 0 11 23 18 535.2 0 18 24 19 535.2 0 19 25 19 535.2 0 19 26 19 535.2 0 19 27 15 535.2 0 15 28 20 535.2 0 20 29 12 535.2 0 12 30 20 535.2 0 20 31 25 535.2 0 25 32 15 535.2 0 15 33 15 535.2 0 15 34 22 535.2 0 22 35 23 535.2 0 23 36 30 535.2 0 30 37 19 535.2 0 19 38 17 535.2 0 17 39 18 535.2 0 18 40 9 535.2 0 9 41 28 535.2 0 28 42 22 535.2 0 22 43 20 535.2 0 20 44 15 535.2 0 15 45 20 535.2 0 20 46 11 535.2 0 11 47 15 535.2 0 15 48 21 535.2 0 21 49 13 535.2 0 13 50 13 535.2 0 13 51 5 535.2 0 5 52 13 535.2 0 13 53 11 535.2 0 11 54 20 535.2 0 20 55 214 535.2 0 214 56 278 535.2 0 278 57 14 535.2 0 14 58 14 535.2 0 14 59 24 535.2 0 24 60 19 535.2 0 19 61 18 535.2 0 18 62 25 535.2 0 25 63 10 535.2 0 10 64 23 535.2 0 23 65 19 535.2 0 19 66 26 535.2 0 26 67 24 535.2 0 24 68 22 535.2 0 22 69 24 535.2 0 24 70 49 535.2 0 49 71 64 535.2 0 64 72 74 535.2 0 74 73 28 535.2 0 28 74 23 535.2 0 23 75 26 535.2 0 26 76 44 535.2 0 44 77 56 535.2 0 56 78 49 535.2 0 49 79 51 535.2 0 51 80 47 535.2 0 47 81 240 535.2 0 240 82 130 535.2 0 130 83 141 535.2 0 141 84 31 535.2 0 31 85 27 535.2 0 27 86 36 535.2 0 36 87 43 535.2 0 43 88 44 535.2 0 44 89 29 535.2 0 29 90 38 535.2 0 38 91 24 535.2 0 24 92 36 535.2 0 36 93 33 535.2 0 33 94 36 535.2 0 36 95 62 535.2 0 62 96 51 535.2 0 51 97 33 535.2 0 33 98 36 535.2 0 36 99 36 535.2 0 36 100 34 535.2 0 34 101 116 535.2 0 116 102 415 535.2 0 415 103 367 535.2 0 367 104 103 535.2 0 103 105 44 535.2 0 44 106 46 535.2 0 46 107 73 535.2 0 73 108 94 535.2 0 94 109 81 535.2 0 81 110 119 535.2 0 119 111 105 535.2 0 105 112 119 535.2 0 119 113 72 535.2 0 72 114 72 535.2 0 72 115 70 535.2 0 70 116 58 535.2 0 58 117 53 535.2 0 53 118 94 535.2 0 94 119 79 535.2 0 79 120 76 535.2 0 76 121 62 535.2 0 62 122 66 535.2 0 66 123 103 535.2 0 103 124 119 535.2 0 119 125 86 535.2 0 86 126 68 535.2 0 68 127 96 535.2 0 96 128 74 535.2 0 74 129 55 535.2 0 55 130 45 535.2 0 45 131 83 535.2 0 83 132 133 535.2 0 133 133 271 535.2 0 271 134 209 535.2 0 209 135 318 535.2 0 318 136 89 535.2 0 89 137 279 535.2 0 279 138 316 535.2 0 316 139 202 535.2 0 202 140 207 535.2 0 207 141 120 535.2 0 120 142 40 535.2 0 40 143 54 535.2 0 54 144 107 535.2 0 107 145 126 535.2 0 126 146 214 535.2 0 214 147 186 535.2 0 186 148 155 535.2 0 155 149 73 535.2 0 73 150 73 535.2 0 73 151 78 535.2 0 78 152 87 535.2 0 87 153 97 535.2 0 97 154 93 535.2 0 93 155 97 535.2 0 97 156 412 535.2 0 412 157 320 535.2 0 320 158 540 535.2 0 540 159 140 535.2 0 140 160 122 535.2 0 122 161 114 535.2 0 114 162 84 535.2 0 84 163 73 535.2 0 73 164 92 535.2 0 92 165 127 535.2 0 127 166 113 535.2 0 113 167 89 535.2 0 89 168 105 535.2 0 105 169 108 535.2 0 108 170 95 535.2 0 95 171 76 535.2 0 76 172 87 535.2 0 87 173 104 535.2 0 104 174 65 535.2 0 65 175 60 535.2 0 60 176 51 535.2 0 51 177 54 535.2 0 54 178 68 535.2 0 68 179 115 535.2 0 115 180 504 535.2 0 504 181 471 535.2 0 471 182 737 535.2 0 737 183 73 535.2 0 73 184 72 535.2 0 72 185 98 535.2 0 98 186 70 535.2 0 70 187 52 535.2 0 52 188 91 535.2 0 91 189 45 535.2 0 45 190 75 535.2 0 75 191 66 535.2 0 66 192 70 535.2 0 70 193 76 535.2 0 76 194 66 535.2 0 66 195 59 535.2 0 59 196 48 535.2 0 48 197 48 535.2 0 48 198 100 535.2 0 100 199 106 535.2 0 106 200 117 535.2 0 117 201 68 535.2 0 68 202 58 535.2 0 58 203 78 535.2 0 78 204 91 535.2 0 91 205 113 535.2 0 113 206 105 535.2 0 105 207 152 535.2 0 152 208 140 535.2 0 140 209 195 535.2 0 195 210 295 535.2 0 295 211 3942 535.2 0 3942 212 3223 535.2 0 3223 213 2999 535.2 0 2999 214 1463 535.2 0 1463 215 574 535.2 0 574 216 68 535.2 0 68 217 61 535.2 0 61 218 89 535.2 0 89 219 132 535.2 0 132 220 121 535.2 0 121 221 98 535.2 0 98 222 59 535.2 0 59 223 161 535.2 0 161 224 432 535.2 0 432 225 567 535.2 0 567 226 344 535.2 0 344 227 74 535.2 0 74 228 72 535.2 0 72 229 77 535.2 0 77 230 82 535.2 0 82 231 63 535.2 0 63 232 63 535.2 0 63 233 47 535.2 0 47 234 62 535.2 0 62 235 67 535.2 0 67 236 90 535.2 0 90 237 94 535.2 0 94 238 98 535.2 0 98 239 115 535.2 0 115 240 81 535.2 0 81 241 64 535.2 0 64 242 65 535.2 0 65 243 75 535.2 0 75 244 82 535.2 0 82 245 82 535.2 0 82 246 84 535.2 0 84 247 73 535.2 0 73 248 69 535.2 0 69 249 47 535.2 0 47 250 47 535.2 0 47 251 57 535.2 0 57 252 71 535.2 0 71 253 115 535.2 0 115 254 93 535.2 0 93 255 85 535.2 0 85 256 162 535.2 0 162 257 111 535.2 0 111 258 109 535.2 0 109 259 104 535.2 0 104 260 131 535.2 0 131 261 83 535.2 0 83 262 94 535.2 0 94 263 93 535.2 0 93 264 78 535.2 0 78 265 115 535.2 0 115 266 183 535.2 0 183 267 249 535.2 0 249 268 253 535.2 0 253 269 157 535.2 0 157 270 544 535.2 0 544 271 1360 535.2 0 1360 272 1227 535.2 0 1227 273 209 535.2 0 209 274 156 535.2 0 156 275 126 535.2 0 126 276 101 535.2 0 101 277 152 535.2 0 152 278 179 535.2 0 179 279 265 535.2 0 265 280 152 535.2 0 152 281 109 535.2 0 109 282 96 535.2 0 96 283 81 535.2 0 81 284 95 535.2 0 95 285 93 535.2 0 93 286 122 535.2 0 122 287 152 535.2 0 152 288 160 535.2 0 160 289 155 535.2 0 155 290 118 535.2 0 118 291 184 535.2 0 184 292 1035 535.2 0 1035 293 2139 535.2 0 2139 294 1724 535.2 0 1724 295 312 535.2 0 312 296 99 535.2 0 99 297 88 535.2 0 88 298 83 535.2 0 83 299 103 535.2 0 103 300 148 535.2 0 148 301 185 535.2 0 185 Finished in 3.97 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 76,288 Reads with adapters: 27,573 (36.1%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 27,541 (36.1%) Total basepairs processed: 20,303,311 bp Total written (filtered): 7,499,501 bp (36.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27573 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 19 0.0 3 0 0 13 5 1 19 313 0.0 3 0 232 40 25 16 20 26611 0.0 4 24473 1663 340 101 34 21 71 0.0 4 13 51 4 1 2 22 12 0.0 4 1 1 4 2 4 23 79 0.0 4 0 0 0 43 36 24 1 0.0 4 0 1 30 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 419 0.0 4 0 0 0 0 419 130 3 0.0 4 0 0 0 1 2 134 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 1 185 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 257 5 0.0 4 0 0 0 0 5 258 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.95 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 27,541 Reads with adapters: 1,835 (6.7%) Reads written (passing filters): 27,541 (100.0%) Total basepairs processed: 7,499,501 bp Total written (filtered): 7,468,918 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1835 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.9% C: 18.1% G: 70.4% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 174 430.3 0 174 4 18 107.6 0 18 5 3 26.9 0 3 9 17 0.1 0 4 13 10 22 0.0 1 1 21 11 1112 0.0 1 61 1051 12 394 0.0 1 11 383 13 20 0.0 1 0 20 14 2 0.0 1 0 2 15 1 0.0 1 0 1 124 1 0.0 1 0 1 149 1 0.0 1 1 152 46 0.0 1 33 13 160 1 0.0 1 1 191 2 0.0 1 0 2 210 16 0.0 1 13 3 222 1 0.0 1 0 1 230 3 0.0 1 3 259 1 0.0 1 1