This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.79 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 146,227 (6.7%) Reads that were too short: 92,798 (4.2%) Reads written (passing filters): 53,429 (2.4%) Total basepairs processed: 659,880,795 bp Total written (filtered): 11,366,048 bp (1.7%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 146227 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 758 535.2 0 758 7 393 535.2 0 393 8 10256 535.2 0 10256 9 9241 535.2 0 9241 10 144 535.2 0 144 11 105 535.2 0 105 12 57 535.2 0 57 13 84 535.2 0 84 14 83 535.2 0 83 15 39 535.2 0 39 16 49 535.2 0 49 17 63 535.2 0 63 18 46 535.2 0 46 19 36 535.2 0 36 20 72 535.2 0 72 21 85 535.2 0 85 22 53 535.2 0 53 23 37 535.2 0 37 24 64 535.2 0 64 25 47 535.2 0 47 26 56 535.2 0 56 27 38 535.2 0 38 28 39 535.2 0 39 29 56 535.2 0 56 30 59 535.2 0 59 31 74 535.2 0 74 32 60 535.2 0 60 33 50 535.2 0 50 34 50 535.2 0 50 35 44 535.2 0 44 36 39 535.2 0 39 37 55 535.2 0 55 38 98 535.2 0 98 39 49 535.2 0 49 40 45 535.2 0 45 41 55 535.2 0 55 42 47 535.2 0 47 43 60 535.2 0 60 44 73 535.2 0 73 45 47 535.2 0 47 46 56 535.2 0 56 47 109 535.2 0 109 48 90 535.2 0 90 49 45 535.2 0 45 50 63 535.2 0 63 51 54 535.2 0 54 52 59 535.2 0 59 53 63 535.2 0 63 54 36 535.2 0 36 55 44 535.2 0 44 56 45 535.2 0 45 57 70 535.2 0 70 58 74 535.2 0 74 59 48 535.2 0 48 60 55 535.2 0 55 61 60 535.2 0 60 62 60 535.2 0 60 63 43 535.2 0 43 64 39 535.2 0 39 65 48 535.2 0 48 66 55 535.2 0 55 67 63 535.2 0 63 68 95 535.2 0 95 69 84 535.2 0 84 70 107 535.2 0 107 71 47 535.2 0 47 72 71 535.2 0 71 73 95 535.2 0 95 74 124 535.2 0 124 75 162 535.2 0 162 76 54 535.2 0 54 77 85 535.2 0 85 78 94 535.2 0 94 79 59 535.2 0 59 80 54 535.2 0 54 81 42 535.2 0 42 82 73 535.2 0 73 83 62 535.2 0 62 84 45 535.2 0 45 85 61 535.2 0 61 86 61 535.2 0 61 87 61 535.2 0 61 88 64 535.2 0 64 89 56 535.2 0 56 90 63 535.2 0 63 91 115 535.2 0 115 92 68 535.2 0 68 93 55 535.2 0 55 94 76 535.2 0 76 95 70 535.2 0 70 96 62 535.2 0 62 97 201 535.2 0 201 98 133 535.2 0 133 99 116 535.2 0 116 100 43 535.2 0 43 101 70 535.2 0 70 102 50 535.2 0 50 103 57 535.2 0 57 104 61 535.2 0 61 105 50 535.2 0 50 106 102 535.2 0 102 107 113 535.2 0 113 108 108 535.2 0 108 109 69 535.2 0 69 110 58 535.2 0 58 111 88 535.2 0 88 112 100 535.2 0 100 113 83 535.2 0 83 114 69 535.2 0 69 115 111 535.2 0 111 116 106 535.2 0 106 117 136 535.2 0 136 118 141 535.2 0 141 119 128 535.2 0 128 120 155 535.2 0 155 121 166 535.2 0 166 122 95 535.2 0 95 123 136 535.2 0 136 124 205 535.2 0 205 125 3966 535.2 0 3966 126 389 535.2 0 389 127 466 535.2 0 466 128 119 535.2 0 119 129 105 535.2 0 105 130 123 535.2 0 123 131 86 535.2 0 86 132 95 535.2 0 95 133 86 535.2 0 86 134 235 535.2 0 235 135 274 535.2 0 274 136 308 535.2 0 308 137 127 535.2 0 127 138 120 535.2 0 120 139 120 535.2 0 120 140 83 535.2 0 83 141 90 535.2 0 90 142 109 535.2 0 109 143 75 535.2 0 75 144 108 535.2 0 108 145 209 535.2 0 209 146 458 535.2 0 458 147 527 535.2 0 527 148 121 535.2 0 121 149 102 535.2 0 102 150 117 535.2 0 117 151 125 535.2 0 125 152 127 535.2 0 127 153 97 535.2 0 97 154 133 535.2 0 133 155 169 535.2 0 169 156 157 535.2 0 157 157 278 535.2 0 278 158 221 535.2 0 221 159 243 535.2 0 243 160 157 535.2 0 157 161 181 535.2 0 181 162 170 535.2 0 170 163 151 535.2 0 151 164 167 535.2 0 167 165 250 535.2 0 250 166 225 535.2 0 225 167 240 535.2 0 240 168 117 535.2 0 117 169 105 535.2 0 105 170 120 535.2 0 120 171 135 535.2 0 135 172 181 535.2 0 181 173 845 535.2 0 845 174 1423 535.2 0 1423 175 1089 535.2 0 1089 176 637 535.2 0 637 177 109 535.2 0 109 178 129 535.2 0 129 179 136 535.2 0 136 180 143 535.2 0 143 181 483 535.2 0 483 182 728 535.2 0 728 183 750 535.2 0 750 184 277 535.2 0 277 185 224 535.2 0 224 186 897 535.2 0 897 187 844 535.2 0 844 188 1198 535.2 0 1198 189 263 535.2 0 263 190 127 535.2 0 127 191 124 535.2 0 124 192 124 535.2 0 124 193 170 535.2 0 170 194 172 535.2 0 172 195 137 535.2 0 137 196 132 535.2 0 132 197 98 535.2 0 98 198 80 535.2 0 80 199 143 535.2 0 143 200 158 535.2 0 158 201 222 535.2 0 222 202 140 535.2 0 140 203 168 535.2 0 168 204 158 535.2 0 158 205 178 535.2 0 178 206 393 535.2 0 393 207 712 535.2 0 712 208 846 535.2 0 846 209 265 535.2 0 265 210 98 535.2 0 98 211 178 535.2 0 178 212 270 535.2 0 270 213 235 535.2 0 235 214 252 535.2 0 252 215 390 535.2 0 390 216 212 535.2 0 212 217 199 535.2 0 199 218 260 535.2 0 260 219 266 535.2 0 266 220 428 535.2 0 428 221 405 535.2 0 405 222 396 535.2 0 396 223 383 535.2 0 383 224 472 535.2 0 472 225 5535 535.2 0 5535 226 11106 535.2 0 11106 227 9331 535.2 0 9331 228 238 535.2 0 238 229 521 535.2 0 521 230 1458 535.2 0 1458 231 2990 535.2 0 2990 232 2628 535.2 0 2628 233 481 535.2 0 481 234 255 535.2 0 255 235 230 535.2 0 230 236 134 535.2 0 134 237 161 535.2 0 161 238 138 535.2 0 138 239 121 535.2 0 121 240 179 535.2 0 179 241 209 535.2 0 209 242 242 535.2 0 242 243 198 535.2 0 198 244 172 535.2 0 172 245 240 535.2 0 240 246 258 535.2 0 258 247 230 535.2 0 230 248 183 535.2 0 183 249 194 535.2 0 194 250 657 535.2 0 657 251 1143 535.2 0 1143 252 1226 535.2 0 1226 253 1466 535.2 0 1466 254 1490 535.2 0 1490 255 867 535.2 0 867 256 96 535.2 0 96 257 116 535.2 0 116 258 126 535.2 0 126 259 149 535.2 0 149 260 164 535.2 0 164 261 225 535.2 0 225 262 194 535.2 0 194 263 219 535.2 0 219 264 200 535.2 0 200 265 260 535.2 0 260 266 145 535.2 0 145 267 191 535.2 0 191 268 284 535.2 0 284 269 243 535.2 0 243 270 181 535.2 0 181 271 271 535.2 0 271 272 252 535.2 0 252 273 356 535.2 0 356 274 294 535.2 0 294 275 489 535.2 0 489 276 296 535.2 0 296 277 298 535.2 0 298 278 478 535.2 0 478 279 1257 535.2 0 1257 280 4943 535.2 0 4943 281 4225 535.2 0 4225 282 3219 535.2 0 3219 283 736 535.2 0 736 284 347 535.2 0 347 285 523 535.2 0 523 286 290 535.2 0 290 287 244 535.2 0 244 288 194 535.2 0 194 289 354 535.2 0 354 290 428 535.2 0 428 291 316 535.2 0 316 292 299 535.2 0 299 293 191 535.2 0 191 294 292 535.2 0 292 295 548 535.2 0 548 296 660 535.2 0 660 297 865 535.2 0 865 298 1119 535.2 0 1119 299 1408 535.2 0 1408 300 2675 535.2 0 2675 301 10553 535.2 0 10553 Finished in 2.44 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 53,429 Reads with adapters: 9,540 (17.9%) Reads that were too short: 40 (0.1%) Reads written (passing filters): 9,500 (17.8%) Total basepairs processed: 11,366,048 bp Total written (filtered): 2,582,749 bp (22.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9540 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 11 0.0 3 0 0 6 3 2 19 116 0.0 3 0 93 7 9 7 20 9274 0.0 4 8433 648 140 34 19 21 31 0.0 4 5 25 0 1 22 5 0.0 4 2 0 2 1 23 45 0.0 4 0 0 0 32 13 24 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 37 2 0.0 4 0 1 0 0 1 40 2 0.0 4 1 0 1 42 1 0.0 4 0 0 0 0 1 49 3 0.0 4 0 0 0 0 3 50 6 0.0 4 0 0 0 0 6 54 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 1 63 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 150 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 1 3 229 3 0.0 4 0 0 0 0 3 234 2 0.0 4 0 0 0 0 2 239 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 9,500 Reads with adapters: 2,234 (23.5%) Reads written (passing filters): 9,500 (100.0%) Total basepairs processed: 2,582,749 bp Total written (filtered): 2,558,671 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2234 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 8.8% G: 85.5% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 148.4 0 2 4 1 37.1 0 1 7 2 0.6 0 2 8 2 0.1 0 2 9 172 0.0 0 27 145 10 1853 0.0 1 129 1724 11 171 0.0 1 5 166 12 7 0.0 1 2 5 13 12 0.0 1 0 12 14 1 0.0 1 0 1 57 1 0.0 1 1 79 1 0.0 1 1 137 1 0.0 1 1 150 1 0.0 1 0 1 151 1 0.0 1 1 208 4 0.0 1 3 1 209 1 0.0 1 0 1 210 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.