This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.08 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 154,967 (7.1%) Reads that were too short: 108,380 (4.9%) Reads written (passing filters): 46,587 (2.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 10,723,256 bp (1.6%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 154967 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 642 535.2 0 642 7 333 535.2 0 333 8 9825 535.2 0 9825 9 11080 535.2 0 11080 10 104 535.2 0 104 11 96 535.2 0 96 12 53 535.2 0 53 13 85 535.2 0 85 14 113 535.2 0 113 15 65 535.2 0 65 16 59 535.2 0 59 17 100 535.2 0 100 18 140 535.2 0 140 19 144 535.2 0 144 20 79 535.2 0 79 21 80 535.2 0 80 22 56 535.2 0 56 23 41 535.2 0 41 24 87 535.2 0 87 25 71 535.2 0 71 26 56 535.2 0 56 27 60 535.2 0 60 28 59 535.2 0 59 29 45 535.2 0 45 30 64 535.2 0 64 31 91 535.2 0 91 32 78 535.2 0 78 33 63 535.2 0 63 34 68 535.2 0 68 35 54 535.2 0 54 36 46 535.2 0 46 37 72 535.2 0 72 38 103 535.2 0 103 39 53 535.2 0 53 40 71 535.2 0 71 41 63 535.2 0 63 42 56 535.2 0 56 43 41 535.2 0 41 44 63 535.2 0 63 45 71 535.2 0 71 46 197 535.2 0 197 47 202 535.2 0 202 48 102 535.2 0 102 49 50 535.2 0 50 50 73 535.2 0 73 51 45 535.2 0 45 52 85 535.2 0 85 53 57 535.2 0 57 54 41 535.2 0 41 55 50 535.2 0 50 56 52 535.2 0 52 57 78 535.2 0 78 58 76 535.2 0 76 59 53 535.2 0 53 60 52 535.2 0 52 61 53 535.2 0 53 62 67 535.2 0 67 63 31 535.2 0 31 64 50 535.2 0 50 65 55 535.2 0 55 66 64 535.2 0 64 67 49 535.2 0 49 68 67 535.2 0 67 69 67 535.2 0 67 70 65 535.2 0 65 71 55 535.2 0 55 72 78 535.2 0 78 73 52 535.2 0 52 74 114 535.2 0 114 75 117 535.2 0 117 76 48 535.2 0 48 77 74 535.2 0 74 78 97 535.2 0 97 79 60 535.2 0 60 80 34 535.2 0 34 81 38 535.2 0 38 82 69 535.2 0 69 83 54 535.2 0 54 84 53 535.2 0 53 85 78 535.2 0 78 86 56 535.2 0 56 87 55 535.2 0 55 88 70 535.2 0 70 89 65 535.2 0 65 90 72 535.2 0 72 91 118 535.2 0 118 92 59 535.2 0 59 93 73 535.2 0 73 94 76 535.2 0 76 95 72 535.2 0 72 96 61 535.2 0 61 97 120 535.2 0 120 98 79 535.2 0 79 99 81 535.2 0 81 100 55 535.2 0 55 101 67 535.2 0 67 102 63 535.2 0 63 103 86 535.2 0 86 104 45 535.2 0 45 105 82 535.2 0 82 106 93 535.2 0 93 107 89 535.2 0 89 108 67 535.2 0 67 109 84 535.2 0 84 110 53 535.2 0 53 111 71 535.2 0 71 112 79 535.2 0 79 113 73 535.2 0 73 114 68 535.2 0 68 115 69 535.2 0 69 116 117 535.2 0 117 117 132 535.2 0 132 118 104 535.2 0 104 119 107 535.2 0 107 120 157 535.2 0 157 121 101 535.2 0 101 122 99 535.2 0 99 123 121 535.2 0 121 124 183 535.2 0 183 125 3405 535.2 0 3405 126 346 535.2 0 346 127 424 535.2 0 424 128 73 535.2 0 73 129 79 535.2 0 79 130 75 535.2 0 75 131 66 535.2 0 66 132 70 535.2 0 70 133 65 535.2 0 65 134 116 535.2 0 116 135 179 535.2 0 179 136 134 535.2 0 134 137 76 535.2 0 76 138 95 535.2 0 95 139 105 535.2 0 105 140 66 535.2 0 66 141 97 535.2 0 97 142 82 535.2 0 82 143 67 535.2 0 67 144 78 535.2 0 78 145 129 535.2 0 129 146 277 535.2 0 277 147 347 535.2 0 347 148 88 535.2 0 88 149 64 535.2 0 64 150 94 535.2 0 94 151 103 535.2 0 103 152 102 535.2 0 102 153 88 535.2 0 88 154 92 535.2 0 92 155 108 535.2 0 108 156 96 535.2 0 96 157 163 535.2 0 163 158 128 535.2 0 128 159 172 535.2 0 172 160 81 535.2 0 81 161 70 535.2 0 70 162 108 535.2 0 108 163 137 535.2 0 137 164 127 535.2 0 127 165 117 535.2 0 117 166 126 535.2 0 126 167 138 535.2 0 138 168 77 535.2 0 77 169 91 535.2 0 91 170 91 535.2 0 91 171 128 535.2 0 128 172 164 535.2 0 164 173 583 535.2 0 583 174 807 535.2 0 807 175 555 535.2 0 555 176 278 535.2 0 278 177 79 535.2 0 79 178 114 535.2 0 114 179 112 535.2 0 112 180 107 535.2 0 107 181 341 535.2 0 341 182 551 535.2 0 551 183 590 535.2 0 590 184 234 535.2 0 234 185 117 535.2 0 117 186 277 535.2 0 277 187 332 535.2 0 332 188 327 535.2 0 327 189 180 535.2 0 180 190 115 535.2 0 115 191 95 535.2 0 95 192 101 535.2 0 101 193 121 535.2 0 121 194 109 535.2 0 109 195 72 535.2 0 72 196 96 535.2 0 96 197 66 535.2 0 66 198 54 535.2 0 54 199 89 535.2 0 89 200 92 535.2 0 92 201 135 535.2 0 135 202 107 535.2 0 107 203 139 535.2 0 139 204 120 535.2 0 120 205 74 535.2 0 74 206 180 535.2 0 180 207 224 535.2 0 224 208 280 535.2 0 280 209 125 535.2 0 125 210 90 535.2 0 90 211 162 535.2 0 162 212 301 535.2 0 301 213 185 535.2 0 185 214 232 535.2 0 232 215 304 535.2 0 304 216 132 535.2 0 132 217 139 535.2 0 139 218 280 535.2 0 280 219 279 535.2 0 279 220 308 535.2 0 308 221 238 535.2 0 238 222 278 535.2 0 278 223 308 535.2 0 308 224 437 535.2 0 437 225 6306 535.2 0 6306 226 14084 535.2 0 14084 227 11866 535.2 0 11866 228 149 535.2 0 149 229 624 535.2 0 624 230 588 535.2 0 588 231 1376 535.2 0 1376 232 577 535.2 0 577 233 243 535.2 0 243 234 127 535.2 0 127 235 150 535.2 0 150 236 66 535.2 0 66 237 104 535.2 0 104 238 85 535.2 0 85 239 79 535.2 0 79 240 92 535.2 0 92 241 100 535.2 0 100 242 124 535.2 0 124 243 82 535.2 0 82 244 122 535.2 0 122 245 118 535.2 0 118 246 147 535.2 0 147 247 150 535.2 0 150 248 107 535.2 0 107 249 135 535.2 0 135 250 659 535.2 0 659 251 1383 535.2 0 1383 252 737 535.2 0 737 253 618 535.2 0 618 254 580 535.2 0 580 255 348 535.2 0 348 256 85 535.2 0 85 257 100 535.2 0 100 258 115 535.2 0 115 259 91 535.2 0 91 260 88 535.2 0 88 261 101 535.2 0 101 262 112 535.2 0 112 263 132 535.2 0 132 264 162 535.2 0 162 265 347 535.2 0 347 266 183 535.2 0 183 267 182 535.2 0 182 268 232 535.2 0 232 269 177 535.2 0 177 270 161 535.2 0 161 271 208 535.2 0 208 272 279 535.2 0 279 273 223 535.2 0 223 274 303 535.2 0 303 275 870 535.2 0 870 276 287 535.2 0 287 277 160 535.2 0 160 278 206 535.2 0 206 279 480 535.2 0 480 280 1805 535.2 0 1805 281 1429 535.2 0 1429 282 1048 535.2 0 1048 283 467 535.2 0 467 284 673 535.2 0 673 285 1132 535.2 0 1132 286 405 535.2 0 405 287 461 535.2 0 461 288 346 535.2 0 346 289 481 535.2 0 481 290 832 535.2 0 832 291 645 535.2 0 645 292 598 535.2 0 598 293 309 535.2 0 309 294 489 535.2 0 489 295 893 535.2 0 893 296 1221 535.2 0 1221 297 1977 535.2 0 1977 298 2358 535.2 0 2358 299 3372 535.2 0 3372 300 6606 535.2 0 6606 301 28701 535.2 0 28701 Finished in 2.03 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 46,587 Reads with adapters: 10,516 (22.6%) Reads that were too short: 43 (0.1%) Reads written (passing filters): 10,473 (22.5%) Total basepairs processed: 10,723,256 bp Total written (filtered): 2,863,101 bp (26.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10516 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 79 0.0 3 0 78 1 17 9831 0.0 3 9648 181 2 18 24 0.0 3 1 22 1 19 10 0.0 3 3 3 3 1 20 358 0.0 3 348 6 0 4 21 1 0.0 3 0 1 22 1 0.0 3 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 37 1 0.0 3 1 38 3 0.0 3 1 2 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 1 46 6 0.0 3 0 0 0 6 49 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 57 0.0 3 0 0 2 55 97 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 118 1 0.0 3 0 0 0 1 119 86 0.0 3 0 0 1 85 126 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 166 3 0.0 3 0 0 0 3 167 1 0.0 3 0 0 0 1 172 1 0.0 3 1 185 1 0.0 3 0 1 186 1 0.0 3 1 190 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 7 0.0 3 0 0 0 7 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 10,473 Reads with adapters: 35 (0.3%) Reads written (passing filters): 10,473 (100.0%) Total basepairs processed: 2,863,101 bp Total written (filtered): 2,862,991 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.1% C: 11.4% G: 11.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 30 163.6 0 30 4 5 40.9 0 5