This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.24 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 136,324 (6.2%) Reads that were too short: 23,544 (1.1%) Reads written (passing filters): 112,780 (5.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 30,795,141 bp (4.7%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 136324 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1790 535.2 0 1790 7 48541 535.2 0 48541 8 40377 535.2 0 40377 9 84 535.2 0 84 10 67 535.2 0 67 11 66 535.2 0 66 12 58 535.2 0 58 13 72 535.2 0 72 14 59 535.2 0 59 15 48 535.2 0 48 16 55 535.2 0 55 17 59 535.2 0 59 18 50 535.2 0 50 19 62 535.2 0 62 20 53 535.2 0 53 21 72 535.2 0 72 22 74 535.2 0 74 23 72 535.2 0 72 24 44 535.2 0 44 25 39 535.2 0 39 26 80 535.2 0 80 27 47 535.2 0 47 28 56 535.2 0 56 29 61 535.2 0 61 30 68 535.2 0 68 31 55 535.2 0 55 32 59 535.2 0 59 33 68 535.2 0 68 34 46 535.2 0 46 35 57 535.2 0 57 36 54 535.2 0 54 37 65 535.2 0 65 38 119 535.2 0 119 39 281 535.2 0 281 40 702 535.2 0 702 41 1377 535.2 0 1377 42 66 535.2 0 66 43 62 535.2 0 62 44 47 535.2 0 47 45 75 535.2 0 75 46 60 535.2 0 60 47 55 535.2 0 55 48 30 535.2 0 30 49 51 535.2 0 51 50 48 535.2 0 48 51 98 535.2 0 98 52 125 535.2 0 125 53 61 535.2 0 61 54 68 535.2 0 68 55 66 535.2 0 66 56 37 535.2 0 37 57 58 535.2 0 58 58 65 535.2 0 65 59 46 535.2 0 46 60 60 535.2 0 60 61 65 535.2 0 65 62 46 535.2 0 46 63 42 535.2 0 42 64 66 535.2 0 66 65 67 535.2 0 67 66 67 535.2 0 67 67 56 535.2 0 56 68 60 535.2 0 60 69 55 535.2 0 55 70 71 535.2 0 71 71 85 535.2 0 85 72 100 535.2 0 100 73 73 535.2 0 73 74 115 535.2 0 115 75 48 535.2 0 48 76 66 535.2 0 66 77 73 535.2 0 73 78 64 535.2 0 64 79 70 535.2 0 70 80 53 535.2 0 53 81 56 535.2 0 56 82 60 535.2 0 60 83 89 535.2 0 89 84 95 535.2 0 95 85 72 535.2 0 72 86 79 535.2 0 79 87 56 535.2 0 56 88 67 535.2 0 67 89 67 535.2 0 67 90 79 535.2 0 79 91 123 535.2 0 123 92 131 535.2 0 131 93 162 535.2 0 162 94 70 535.2 0 70 95 81 535.2 0 81 96 74 535.2 0 74 97 96 535.2 0 96 98 386 535.2 0 386 99 663 535.2 0 663 100 586 535.2 0 586 101 98 535.2 0 98 102 122 535.2 0 122 103 90 535.2 0 90 104 74 535.2 0 74 105 75 535.2 0 75 106 61 535.2 0 61 107 85 535.2 0 85 108 74 535.2 0 74 109 101 535.2 0 101 110 87 535.2 0 87 111 338 535.2 0 338 112 313 535.2 0 313 113 251 535.2 0 251 114 90 535.2 0 90 115 93 535.2 0 93 116 86 535.2 0 86 117 81 535.2 0 81 118 73 535.2 0 73 119 62 535.2 0 62 120 92 535.2 0 92 121 82 535.2 0 82 122 72 535.2 0 72 123 88 535.2 0 88 124 112 535.2 0 112 125 111 535.2 0 111 126 69 535.2 0 69 127 71 535.2 0 71 128 58 535.2 0 58 129 68 535.2 0 68 130 81 535.2 0 81 131 58 535.2 0 58 132 88 535.2 0 88 133 105 535.2 0 105 134 103 535.2 0 103 135 98 535.2 0 98 136 98 535.2 0 98 137 128 535.2 0 128 138 106 535.2 0 106 139 159 535.2 0 159 140 82 535.2 0 82 141 70 535.2 0 70 142 96 535.2 0 96 143 135 535.2 0 135 144 127 535.2 0 127 145 103 535.2 0 103 146 140 535.2 0 140 147 150 535.2 0 150 148 133 535.2 0 133 149 80 535.2 0 80 150 102 535.2 0 102 151 86 535.2 0 86 152 80 535.2 0 80 153 89 535.2 0 89 154 86 535.2 0 86 155 91 535.2 0 91 156 97 535.2 0 97 157 86 535.2 0 86 158 193 535.2 0 193 159 156 535.2 0 156 160 154 535.2 0 154 161 67 535.2 0 67 162 98 535.2 0 98 163 122 535.2 0 122 164 140 535.2 0 140 165 83 535.2 0 83 166 96 535.2 0 96 167 98 535.2 0 98 168 95 535.2 0 95 169 117 535.2 0 117 170 134 535.2 0 134 171 174 535.2 0 174 172 223 535.2 0 223 173 152 535.2 0 152 174 81 535.2 0 81 175 55 535.2 0 55 176 92 535.2 0 92 177 184 535.2 0 184 178 236 535.2 0 236 179 246 535.2 0 246 180 180 535.2 0 180 181 109 535.2 0 109 182 108 535.2 0 108 183 298 535.2 0 298 184 376 535.2 0 376 185 314 535.2 0 314 186 129 535.2 0 129 187 125 535.2 0 125 188 150 535.2 0 150 189 206 535.2 0 206 190 137 535.2 0 137 191 137 535.2 0 137 192 148 535.2 0 148 193 96 535.2 0 96 194 90 535.2 0 90 195 126 535.2 0 126 196 114 535.2 0 114 197 81 535.2 0 81 198 64 535.2 0 64 199 115 535.2 0 115 200 93 535.2 0 93 201 105 535.2 0 105 202 97 535.2 0 97 203 118 535.2 0 118 204 117 535.2 0 117 205 80 535.2 0 80 206 95 535.2 0 95 207 107 535.2 0 107 208 91 535.2 0 91 209 104 535.2 0 104 210 154 535.2 0 154 211 169 535.2 0 169 212 199 535.2 0 199 213 145 535.2 0 145 214 388 535.2 0 388 215 796 535.2 0 796 216 593 535.2 0 593 217 181 535.2 0 181 218 82 535.2 0 82 219 94 535.2 0 94 220 168 535.2 0 168 221 115 535.2 0 115 222 209 535.2 0 209 223 799 535.2 0 799 224 853 535.2 0 853 225 633 535.2 0 633 226 95 535.2 0 95 227 105 535.2 0 105 228 166 535.2 0 166 229 182 535.2 0 182 230 124 535.2 0 124 231 100 535.2 0 100 232 97 535.2 0 97 233 90 535.2 0 90 234 76 535.2 0 76 235 86 535.2 0 86 236 125 535.2 0 125 237 145 535.2 0 145 238 401 535.2 0 401 239 327 535.2 0 327 240 392 535.2 0 392 241 139 535.2 0 139 242 176 535.2 0 176 243 122 535.2 0 122 244 72 535.2 0 72 245 102 535.2 0 102 246 93 535.2 0 93 247 122 535.2 0 122 248 85 535.2 0 85 249 120 535.2 0 120 250 111 535.2 0 111 251 130 535.2 0 130 252 145 535.2 0 145 253 138 535.2 0 138 254 174 535.2 0 174 255 94 535.2 0 94 256 180 535.2 0 180 257 176 535.2 0 176 258 190 535.2 0 190 259 146 535.2 0 146 260 200 535.2 0 200 261 189 535.2 0 189 262 124 535.2 0 124 263 308 535.2 0 308 264 458 535.2 0 458 265 357 535.2 0 357 266 109 535.2 0 109 267 117 535.2 0 117 268 77 535.2 0 77 269 113 535.2 0 113 270 134 535.2 0 134 271 260 535.2 0 260 272 1607 535.2 0 1607 273 999 535.2 0 999 274 1351 535.2 0 1351 275 152 535.2 0 152 276 209 535.2 0 209 277 383 535.2 0 383 278 467 535.2 0 467 279 181 535.2 0 181 280 224 535.2 0 224 281 113 535.2 0 113 282 139 535.2 0 139 283 287 535.2 0 287 284 448 535.2 0 448 285 549 535.2 0 549 286 319 535.2 0 319 287 110 535.2 0 110 288 131 535.2 0 131 289 96 535.2 0 96 290 94 535.2 0 94 291 94 535.2 0 94 292 109 535.2 0 109 293 96 535.2 0 96 294 102 535.2 0 102 295 94 535.2 0 94 296 84 535.2 0 84 297 137 535.2 0 137 298 240 535.2 0 240 299 391 535.2 0 391 300 288 535.2 0 288 301 191 535.2 0 191 Finished in 5.91 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 112,780 Reads with adapters: 41,495 (36.8%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 41,449 (36.8%) Total basepairs processed: 30,795,141 bp Total written (filtered): 11,302,176 bp (36.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41495 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 1 4 18 26 0.0 3 0 0 16 7 3 19 613 0.0 3 0 416 94 70 33 20 40329 0.0 4 36240 2990 773 246 80 21 129 0.0 4 29 86 6 4 4 22 13 0.0 4 0 0 10 2 1 23 194 0.0 4 0 0 0 131 63 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 1 34 1 0.0 4 0 0 0 0 1 36 7 0.0 4 0 0 0 0 7 37 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 1 50 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 1 72 1 0.0 4 0 1 73 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 107 0.0 4 0 0 0 0 107 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 7 0.0 4 0 0 0 0 7 163 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 205 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 234 4 0.0 4 0 0 0 0 4 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 5 0.0 4 0 0 0 0 5 238 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.47 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 41,449 Reads with adapters: 7,002 (16.9%) Reads written (passing filters): 41,449 (100.0%) Total basepairs processed: 11,302,176 bp Total written (filtered): 11,204,156 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7002 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.3% C: 10.7% G: 82.6% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 98 647.6 0 98 4 19 161.9 0 19 5 2 40.5 0 2 6 1 10.1 0 1 7 2 2.5 0 2 8 2 0.6 0 2 9 42 0.2 0 6 36 10 1209 0.0 1 110 1099 11 5341 0.0 1 237 5104 12 133 0.0 1 4 129 13 8 0.0 1 0 8 14 11 0.0 1 0 11 15 1 0.0 1 0 1 41 1 0.0 1 1 73 1 0.0 1 0 1 148 3 0.0 1 2 1 151 48 0.0 1 36 12 152 1 0.0 1 0 1 156 1 0.0 1 1 168 1 0.0 1 1 178 1 0.0 1 1 190 3 0.0 1 0 3 208 1 0.0 1 1 209 50 0.0 1 43 7 210 5 0.0 1 5 211 4 0.0 1 2 2 221 1 0.0 1 0 1 224 5 0.0 1 4 1 225 2 0.0 1 2 227 1 0.0 1 1 229 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.