This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.85 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 135,674 (6.2%) Reads that were too short: 18,622 (0.8%) Reads written (passing filters): 117,052 (5.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 32,222,570 bp (4.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 135674 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1392 535.2 0 1392 7 42551 535.2 0 42551 8 51973 535.2 0 51973 9 115 535.2 0 115 10 102 535.2 0 102 11 82 535.2 0 82 12 79 535.2 0 79 13 90 535.2 0 90 14 73 535.2 0 73 15 78 535.2 0 78 16 69 535.2 0 69 17 80 535.2 0 80 18 87 535.2 0 87 19 88 535.2 0 88 20 81 535.2 0 81 21 76 535.2 0 76 22 103 535.2 0 103 23 82 535.2 0 82 24 62 535.2 0 62 25 63 535.2 0 63 26 80 535.2 0 80 27 88 535.2 0 88 28 82 535.2 0 82 29 89 535.2 0 89 30 94 535.2 0 94 31 80 535.2 0 80 32 87 535.2 0 87 33 84 535.2 0 84 34 60 535.2 0 60 35 106 535.2 0 106 36 88 535.2 0 88 37 73 535.2 0 73 38 101 535.2 0 101 39 186 535.2 0 186 40 327 535.2 0 327 41 397 535.2 0 397 42 74 535.2 0 74 43 95 535.2 0 95 44 59 535.2 0 59 45 80 535.2 0 80 46 93 535.2 0 93 47 69 535.2 0 69 48 74 535.2 0 74 49 81 535.2 0 81 50 74 535.2 0 74 51 74 535.2 0 74 52 106 535.2 0 106 53 95 535.2 0 95 54 103 535.2 0 103 55 75 535.2 0 75 56 95 535.2 0 95 57 96 535.2 0 96 58 97 535.2 0 97 59 71 535.2 0 71 60 99 535.2 0 99 61 63 535.2 0 63 62 72 535.2 0 72 63 74 535.2 0 74 64 77 535.2 0 77 65 291 535.2 0 291 66 569 535.2 0 569 67 94 535.2 0 94 68 99 535.2 0 99 69 77 535.2 0 77 70 81 535.2 0 81 71 97 535.2 0 97 72 124 535.2 0 124 73 103 535.2 0 103 74 117 535.2 0 117 75 91 535.2 0 91 76 74 535.2 0 74 77 88 535.2 0 88 78 97 535.2 0 97 79 90 535.2 0 90 80 111 535.2 0 111 81 87 535.2 0 87 82 85 535.2 0 85 83 88 535.2 0 88 84 98 535.2 0 98 85 90 535.2 0 90 86 86 535.2 0 86 87 72 535.2 0 72 88 67 535.2 0 67 89 65 535.2 0 65 90 78 535.2 0 78 91 108 535.2 0 108 92 114 535.2 0 114 93 119 535.2 0 119 94 69 535.2 0 69 95 93 535.2 0 93 96 94 535.2 0 94 97 92 535.2 0 92 98 207 535.2 0 207 99 298 535.2 0 298 100 283 535.2 0 283 101 90 535.2 0 90 102 150 535.2 0 150 103 88 535.2 0 88 104 88 535.2 0 88 105 95 535.2 0 95 106 82 535.2 0 82 107 116 535.2 0 116 108 98 535.2 0 98 109 109 535.2 0 109 110 93 535.2 0 93 111 291 535.2 0 291 112 241 535.2 0 241 113 177 535.2 0 177 114 97 535.2 0 97 115 79 535.2 0 79 116 108 535.2 0 108 117 86 535.2 0 86 118 78 535.2 0 78 119 70 535.2 0 70 120 81 535.2 0 81 121 99 535.2 0 99 122 76 535.2 0 76 123 74 535.2 0 74 124 122 535.2 0 122 125 116 535.2 0 116 126 97 535.2 0 97 127 87 535.2 0 87 128 82 535.2 0 82 129 76 535.2 0 76 130 82 535.2 0 82 131 115 535.2 0 115 132 87 535.2 0 87 133 88 535.2 0 88 134 93 535.2 0 93 135 126 535.2 0 126 136 88 535.2 0 88 137 90 535.2 0 90 138 88 535.2 0 88 139 87 535.2 0 87 140 71 535.2 0 71 141 56 535.2 0 56 142 99 535.2 0 99 143 108 535.2 0 108 144 127 535.2 0 127 145 86 535.2 0 86 146 125 535.2 0 125 147 155 535.2 0 155 148 160 535.2 0 160 149 116 535.2 0 116 150 96 535.2 0 96 151 94 535.2 0 94 152 95 535.2 0 95 153 99 535.2 0 99 154 74 535.2 0 74 155 90 535.2 0 90 156 99 535.2 0 99 157 94 535.2 0 94 158 145 535.2 0 145 159 146 535.2 0 146 160 159 535.2 0 159 161 79 535.2 0 79 162 95 535.2 0 95 163 103 535.2 0 103 164 97 535.2 0 97 165 88 535.2 0 88 166 95 535.2 0 95 167 102 535.2 0 102 168 95 535.2 0 95 169 107 535.2 0 107 170 107 535.2 0 107 171 120 535.2 0 120 172 153 535.2 0 153 173 153 535.2 0 153 174 92 535.2 0 92 175 73 535.2 0 73 176 92 535.2 0 92 177 129 535.2 0 129 178 137 535.2 0 137 179 151 535.2 0 151 180 137 535.2 0 137 181 98 535.2 0 98 182 98 535.2 0 98 183 274 535.2 0 274 184 378 535.2 0 378 185 262 535.2 0 262 186 115 535.2 0 115 187 103 535.2 0 103 188 97 535.2 0 97 189 122 535.2 0 122 190 109 535.2 0 109 191 130 535.2 0 130 192 118 535.2 0 118 193 99 535.2 0 99 194 79 535.2 0 79 195 104 535.2 0 104 196 96 535.2 0 96 197 87 535.2 0 87 198 72 535.2 0 72 199 88 535.2 0 88 200 91 535.2 0 91 201 103 535.2 0 103 202 82 535.2 0 82 203 94 535.2 0 94 204 113 535.2 0 113 205 85 535.2 0 85 206 71 535.2 0 71 207 96 535.2 0 96 208 59 535.2 0 59 209 61 535.2 0 61 210 108 535.2 0 108 211 157 535.2 0 157 212 127 535.2 0 127 213 80 535.2 0 80 214 173 535.2 0 173 215 317 535.2 0 317 216 229 535.2 0 229 217 124 535.2 0 124 218 67 535.2 0 67 219 87 535.2 0 87 220 162 535.2 0 162 221 104 535.2 0 104 222 153 535.2 0 153 223 720 535.2 0 720 224 864 535.2 0 864 225 698 535.2 0 698 226 86 535.2 0 86 227 98 535.2 0 98 228 162 535.2 0 162 229 158 535.2 0 158 230 79 535.2 0 79 231 96 535.2 0 96 232 92 535.2 0 92 233 69 535.2 0 69 234 89 535.2 0 89 235 90 535.2 0 90 236 91 535.2 0 91 237 102 535.2 0 102 238 423 535.2 0 423 239 305 535.2 0 305 240 230 535.2 0 230 241 110 535.2 0 110 242 122 535.2 0 122 243 102 535.2 0 102 244 61 535.2 0 61 245 108 535.2 0 108 246 78 535.2 0 78 247 83 535.2 0 83 248 89 535.2 0 89 249 97 535.2 0 97 250 93 535.2 0 93 251 92 535.2 0 92 252 83 535.2 0 83 253 95 535.2 0 95 254 153 535.2 0 153 255 80 535.2 0 80 256 144 535.2 0 144 257 107 535.2 0 107 258 85 535.2 0 85 259 108 535.2 0 108 260 149 535.2 0 149 261 173 535.2 0 173 262 100 535.2 0 100 263 243 535.2 0 243 264 279 535.2 0 279 265 300 535.2 0 300 266 90 535.2 0 90 267 131 535.2 0 131 268 79 535.2 0 79 269 109 535.2 0 109 270 108 535.2 0 108 271 218 535.2 0 218 272 1619 535.2 0 1619 273 932 535.2 0 932 274 1312 535.2 0 1312 275 128 535.2 0 128 276 101 535.2 0 101 277 181 535.2 0 181 278 310 535.2 0 310 279 148 535.2 0 148 280 165 535.2 0 165 281 85 535.2 0 85 282 99 535.2 0 99 283 152 535.2 0 152 284 242 535.2 0 242 285 333 535.2 0 333 286 206 535.2 0 206 287 82 535.2 0 82 288 117 535.2 0 117 289 103 535.2 0 103 290 88 535.2 0 88 291 60 535.2 0 60 292 88 535.2 0 88 293 68 535.2 0 68 294 88 535.2 0 88 295 102 535.2 0 102 296 65 535.2 0 65 297 135 535.2 0 135 298 151 535.2 0 151 299 291 535.2 0 291 300 190 535.2 0 190 301 111 535.2 0 111 Finished in 5.42 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 117,052 Reads with adapters: 42,460 (36.3%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 42,431 (36.2%) Total basepairs processed: 32,222,570 bp Total written (filtered): 11,747,133 bp (36.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 42460 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 10 16 192 0.0 3 0 184 5 3 17 42016 0.0 3 41052 937 27 18 97 0.0 3 10 86 1 19 17 0.0 3 1 0 14 2 20 52 0.0 3 1 0 0 51 35 1 0.0 3 1 37 1 0.0 3 1 45 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 80 4 0.0 3 0 0 0 4 81 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 8 0.0 3 0 0 0 8 133 3 0.0 3 1 0 0 2 134 1 0.0 3 0 0 0 1 135 5 0.0 3 0 0 0 5 137 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 3 0.0 3 0 0 0 3 156 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 205 3 0.0 3 0 0 0 3 212 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 4 0.0 3 0 0 0 4 279 2 0.0 3 0 0 0 2 281 4 0.0 3 0 0 1 3 282 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.48 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 42,431 Reads with adapters: 118 (0.3%) Reads written (passing filters): 42,431 (100.0%) Total basepairs processed: 11,747,133 bp Total written (filtered): 11,746,762 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 118 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.5% C: 23.7% G: 13.6% T: 43.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 107 663.0 0 107 4 6 165.7 0 6 5 4 41.4 0 4 6 1 10.4 0 1