This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.67 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 242,246 (11.0%) Reads that were too short: 22,391 (1.0%) Reads written (passing filters): 219,855 (10.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 41,932,583 bp (6.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 242246 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 652 535.2 0 652 7 8931 535.2 0 8931 8 486 535.2 0 486 9 10059 535.2 0 10059 10 163 535.2 0 163 11 193 535.2 0 193 12 153 535.2 0 153 13 134 535.2 0 134 14 136 535.2 0 136 15 140 535.2 0 140 16 148 535.2 0 148 17 184 535.2 0 184 18 172 535.2 0 172 19 154 535.2 0 154 20 183 535.2 0 183 21 431 535.2 0 431 22 556 535.2 0 556 23 490 535.2 0 490 24 1238 535.2 0 1238 25 2250 535.2 0 2250 26 2596 535.2 0 2596 27 150 535.2 0 150 28 166 535.2 0 166 29 137 535.2 0 137 30 124 535.2 0 124 31 144 535.2 0 144 32 136 535.2 0 136 33 133 535.2 0 133 34 130 535.2 0 130 35 103 535.2 0 103 36 141 535.2 0 141 37 107 535.2 0 107 38 118 535.2 0 118 39 127 535.2 0 127 40 131 535.2 0 131 41 146 535.2 0 146 42 115 535.2 0 115 43 126 535.2 0 126 44 140 535.2 0 140 45 131 535.2 0 131 46 142 535.2 0 142 47 126 535.2 0 126 48 144 535.2 0 144 49 127 535.2 0 127 50 135 535.2 0 135 51 166 535.2 0 166 52 121 535.2 0 121 53 143 535.2 0 143 54 198 535.2 0 198 55 212 535.2 0 212 56 157 535.2 0 157 57 164 535.2 0 164 58 181 535.2 0 181 59 154 535.2 0 154 60 143 535.2 0 143 61 533 535.2 0 533 62 1095 535.2 0 1095 63 700 535.2 0 700 64 136 535.2 0 136 65 118 535.2 0 118 66 157 535.2 0 157 67 162 535.2 0 162 68 152 535.2 0 152 69 283 535.2 0 283 70 415 535.2 0 415 71 371 535.2 0 371 72 227 535.2 0 227 73 190 535.2 0 190 74 239 535.2 0 239 75 175 535.2 0 175 76 163 535.2 0 163 77 185 535.2 0 185 78 231 535.2 0 231 79 241 535.2 0 241 80 279 535.2 0 279 81 259 535.2 0 259 82 418 535.2 0 418 83 1936 535.2 0 1936 84 3582 535.2 0 3582 85 2605 535.2 0 2605 86 230 535.2 0 230 87 185 535.2 0 185 88 309 535.2 0 309 89 159 535.2 0 159 90 186 535.2 0 186 91 133 535.2 0 133 92 154 535.2 0 154 93 148 535.2 0 148 94 193 535.2 0 193 95 193 535.2 0 193 96 175 535.2 0 175 97 204 535.2 0 204 98 319 535.2 0 319 99 623 535.2 0 623 100 239 535.2 0 239 101 270 535.2 0 270 102 377 535.2 0 377 103 271 535.2 0 271 104 246 535.2 0 246 105 440 535.2 0 440 106 449 535.2 0 449 107 595 535.2 0 595 108 675 535.2 0 675 109 887 535.2 0 887 110 1011 535.2 0 1011 111 1312 535.2 0 1312 112 19222 535.2 0 19222 113 41113 535.2 0 41113 114 32051 535.2 0 32051 115 293 535.2 0 293 116 145 535.2 0 145 117 201 535.2 0 201 118 286 535.2 0 286 119 346 535.2 0 346 120 2094 535.2 0 2094 121 2200 535.2 0 2200 122 2477 535.2 0 2477 123 274 535.2 0 274 124 298 535.2 0 298 125 951 535.2 0 951 126 1067 535.2 0 1067 127 1109 535.2 0 1109 128 238 535.2 0 238 129 421 535.2 0 421 130 303 535.2 0 303 131 317 535.2 0 317 132 143 535.2 0 143 133 173 535.2 0 173 134 195 535.2 0 195 135 176 535.2 0 176 136 234 535.2 0 234 137 357 535.2 0 357 138 374 535.2 0 374 139 351 535.2 0 351 140 291 535.2 0 291 141 262 535.2 0 262 142 693 535.2 0 693 143 1164 535.2 0 1164 144 925 535.2 0 925 145 404 535.2 0 404 146 434 535.2 0 434 147 1627 535.2 0 1627 148 2916 535.2 0 2916 149 3005 535.2 0 3005 150 904 535.2 0 904 151 335 535.2 0 335 152 388 535.2 0 388 153 217 535.2 0 217 154 159 535.2 0 159 155 179 535.2 0 179 156 186 535.2 0 186 157 189 535.2 0 189 158 194 535.2 0 194 159 192 535.2 0 192 160 237 535.2 0 237 161 272 535.2 0 272 162 342 535.2 0 342 163 324 535.2 0 324 164 449 535.2 0 449 165 579 535.2 0 579 166 1042 535.2 0 1042 167 2023 535.2 0 2023 168 2318 535.2 0 2318 169 957 535.2 0 957 170 884 535.2 0 884 171 1999 535.2 0 1999 172 2053 535.2 0 2053 173 2192 535.2 0 2192 174 476 535.2 0 476 175 321 535.2 0 321 176 277 535.2 0 277 177 223 535.2 0 223 178 237 535.2 0 237 179 198 535.2 0 198 180 229 535.2 0 229 181 273 535.2 0 273 182 404 535.2 0 404 183 2162 535.2 0 2162 184 2371 535.2 0 2371 185 2256 535.2 0 2256 186 635 535.2 0 635 187 479 535.2 0 479 188 539 535.2 0 539 189 454 535.2 0 454 190 291 535.2 0 291 191 1341 535.2 0 1341 192 2620 535.2 0 2620 193 2766 535.2 0 2766 194 953 535.2 0 953 195 381 535.2 0 381 196 428 535.2 0 428 197 231 535.2 0 231 198 242 535.2 0 242 199 1467 535.2 0 1467 200 397 535.2 0 397 201 358 535.2 0 358 202 244 535.2 0 244 203 223 535.2 0 223 204 147 535.2 0 147 205 179 535.2 0 179 206 209 535.2 0 209 207 231 535.2 0 231 208 286 535.2 0 286 209 205 535.2 0 205 210 189 535.2 0 189 211 150 535.2 0 150 212 175 535.2 0 175 213 162 535.2 0 162 214 140 535.2 0 140 215 188 535.2 0 188 216 242 535.2 0 242 217 196 535.2 0 196 218 267 535.2 0 267 219 310 535.2 0 310 220 240 535.2 0 240 221 156 535.2 0 156 222 178 535.2 0 178 223 156 535.2 0 156 224 185 535.2 0 185 225 269 535.2 0 269 226 416 535.2 0 416 227 363 535.2 0 363 228 572 535.2 0 572 229 390 535.2 0 390 230 314 535.2 0 314 231 164 535.2 0 164 232 167 535.2 0 167 233 154 535.2 0 154 234 159 535.2 0 159 235 175 535.2 0 175 236 132 535.2 0 132 237 178 535.2 0 178 238 196 535.2 0 196 239 211 535.2 0 211 240 161 535.2 0 161 241 141 535.2 0 141 242 161 535.2 0 161 243 182 535.2 0 182 244 231 535.2 0 231 245 261 535.2 0 261 246 461 535.2 0 461 247 356 535.2 0 356 248 192 535.2 0 192 249 134 535.2 0 134 250 133 535.2 0 133 251 240 535.2 0 240 252 212 535.2 0 212 253 230 535.2 0 230 254 179 535.2 0 179 255 212 535.2 0 212 256 258 535.2 0 258 257 204 535.2 0 204 258 208 535.2 0 208 259 162 535.2 0 162 260 167 535.2 0 167 261 172 535.2 0 172 262 170 535.2 0 170 263 179 535.2 0 179 264 168 535.2 0 168 265 191 535.2 0 191 266 198 535.2 0 198 267 711 535.2 0 711 268 907 535.2 0 907 269 591 535.2 0 591 270 209 535.2 0 209 271 153 535.2 0 153 272 143 535.2 0 143 273 145 535.2 0 145 274 139 535.2 0 139 275 176 535.2 0 176 276 205 535.2 0 205 277 295 535.2 0 295 278 248 535.2 0 248 279 278 535.2 0 278 280 191 535.2 0 191 281 194 535.2 0 194 282 230 535.2 0 230 283 160 535.2 0 160 284 107 535.2 0 107 285 116 535.2 0 116 286 133 535.2 0 133 287 147 535.2 0 147 288 138 535.2 0 138 289 181 535.2 0 181 290 146 535.2 0 146 291 143 535.2 0 143 292 139 535.2 0 139 293 183 535.2 0 183 294 212 535.2 0 212 295 206 535.2 0 206 296 162 535.2 0 162 297 312 535.2 0 312 298 279 535.2 0 279 299 427 535.2 0 427 300 134 535.2 0 134 301 170 535.2 0 170 Finished in 8.80 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 219,855 Reads with adapters: 9,358 (4.3%) Reads that were too short: 195 (0.1%) Reads written (passing filters): 9,163 (4.2%) Total basepairs processed: 41,932,583 bp Total written (filtered): 2,484,546 bp (5.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9358 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 107 0.0 3 0 81 12 12 2 20 8693 0.0 4 7999 536 98 49 11 21 79 0.0 4 39 28 5 6 1 22 9 0.0 4 1 0 2 0 6 23 36 0.0 4 5 1 0 21 9 24 10 0.0 4 0 0 0 0 10 25 4 0.0 4 0 0 0 2 2 28 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 36 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 6 0.0 4 0 0 0 0 6 50 5 0.0 4 0 0 0 0 5 51 78 0.0 4 0 0 0 0 78 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 71 3 0.0 4 0 0 0 0 3 72 2 0.0 4 0 0 0 0 2 74 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 4 0.0 4 0 0 0 0 4 79 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 95 2 0.0 4 0 0 0 0 2 96 2 0.0 4 0 0 0 0 2 98 3 0.0 4 0 0 0 0 3 99 2 0.0 4 0 0 0 0 2 100 2 0.0 4 0 0 0 0 2 101 4 0.0 4 0 0 0 1 3 102 1 0.0 4 0 0 0 0 1 103 4 0.0 4 0 0 0 0 4 108 2 0.0 4 0 0 0 0 2 110 27 0.0 4 0 0 0 0 27 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 7 0.0 4 0 0 0 0 7 115 2 0.0 4 0 0 0 1 1 119 1 0.0 4 0 0 0 0 1 120 3 0.0 4 0 0 0 0 3 123 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 86 0.0 4 0 0 0 0 86 128 4 0.0 4 0 0 0 0 4 129 7 0.0 4 0 0 0 0 7 130 3 0.0 4 0 0 0 0 3 131 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 139 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 149 2 0.0 4 0 0 0 0 2 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 157 2 0.0 4 0 0 0 0 2 158 4 0.0 4 0 0 0 0 4 159 3 0.0 4 0 0 0 0 3 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 1 168 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 31 0.0 4 0 0 0 1 30 178 13 0.0 4 0 0 0 1 12 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 0 0 0 2 182 1 0.0 4 0 0 0 0 1 187 2 0.0 4 0 0 0 0 2 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 1 191 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 198 2 0.0 4 0 0 0 0 2 201 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 1 210 2 0.0 4 0 0 0 0 2 212 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 220 2 0.0 4 0 0 0 0 2 221 2 0.0 4 0 0 0 1 1 228 3 0.0 4 0 0 0 0 3 242 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 1 261 1 0.0 4 0 0 0 0 1 262 3 0.0 4 0 0 0 0 3 265 2 0.0 4 0 0 0 0 2 267 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 9,163 Reads with adapters: 901 (9.8%) Reads written (passing filters): 9,163 (100.0%) Total basepairs processed: 2,484,546 bp Total written (filtered): 2,427,231 bp (97.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 901 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.1% C: 10.5% G: 38.3% T: 45.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 58 143.2 0 58 4 20 35.8 0 20 6 1 2.2 0 1 9 4 0.0 0 0 4 10 13 0.0 1 1 12 11 279 0.0 1 10 269 12 113 0.0 1 4 109 13 3 0.0 1 0 3 14 1 0.0 1 0 1 15 1 0.0 1 0 1 127 381 0.0 1 322 59 128 16 0.0 1 12 4 129 1 0.0 1 1 152 4 0.0 1 3 1 172 1 0.0 1 1 210 4 0.0 1 3 1 212 1 0.0 1 1