This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.69 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 173,554 (7.9%) Reads that were too short: 42,480 (1.9%) Reads written (passing filters): 131,074 (6.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 35,522,091 bp (5.4%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 173554 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 573 535.2 0 573 7 1418 535.2 0 1418 8 59739 535.2 0 59739 9 42296 535.2 0 42296 10 173 535.2 0 173 11 101 535.2 0 101 12 111 535.2 0 111 13 97 535.2 0 97 14 133 535.2 0 133 15 128 535.2 0 128 16 123 535.2 0 123 17 142 535.2 0 142 18 121 535.2 0 121 19 108 535.2 0 108 20 121 535.2 0 121 21 108 535.2 0 108 22 128 535.2 0 128 23 109 535.2 0 109 24 103 535.2 0 103 25 95 535.2 0 95 26 137 535.2 0 137 27 126 535.2 0 126 28 114 535.2 0 114 29 128 535.2 0 128 30 118 535.2 0 118 31 101 535.2 0 101 32 105 535.2 0 105 33 122 535.2 0 122 34 120 535.2 0 120 35 108 535.2 0 108 36 106 535.2 0 106 37 123 535.2 0 123 38 100 535.2 0 100 39 95 535.2 0 95 40 88 535.2 0 88 41 98 535.2 0 98 42 103 535.2 0 103 43 112 535.2 0 112 44 159 535.2 0 159 45 111 535.2 0 111 46 116 535.2 0 116 47 107 535.2 0 107 48 116 535.2 0 116 49 107 535.2 0 107 50 111 535.2 0 111 51 109 535.2 0 109 52 129 535.2 0 129 53 136 535.2 0 136 54 129 535.2 0 129 55 101 535.2 0 101 56 107 535.2 0 107 57 101 535.2 0 101 58 108 535.2 0 108 59 139 535.2 0 139 60 188 535.2 0 188 61 171 535.2 0 171 62 108 535.2 0 108 63 105 535.2 0 105 64 97 535.2 0 97 65 92 535.2 0 92 66 112 535.2 0 112 67 125 535.2 0 125 68 86 535.2 0 86 69 102 535.2 0 102 70 97 535.2 0 97 71 134 535.2 0 134 72 170 535.2 0 170 73 182 535.2 0 182 74 108 535.2 0 108 75 119 535.2 0 119 76 186 535.2 0 186 77 258 535.2 0 258 78 308 535.2 0 308 79 105 535.2 0 105 80 143 535.2 0 143 81 138 535.2 0 138 82 131 535.2 0 131 83 128 535.2 0 128 84 132 535.2 0 132 85 162 535.2 0 162 86 95 535.2 0 95 87 133 535.2 0 133 88 115 535.2 0 115 89 119 535.2 0 119 90 123 535.2 0 123 91 107 535.2 0 107 92 149 535.2 0 149 93 163 535.2 0 163 94 125 535.2 0 125 95 164 535.2 0 164 96 137 535.2 0 137 97 92 535.2 0 92 98 128 535.2 0 128 99 91 535.2 0 91 100 131 535.2 0 131 101 114 535.2 0 114 102 106 535.2 0 106 103 103 535.2 0 103 104 113 535.2 0 113 105 131 535.2 0 131 106 112 535.2 0 112 107 118 535.2 0 118 108 112 535.2 0 112 109 134 535.2 0 134 110 175 535.2 0 175 111 204 535.2 0 204 112 269 535.2 0 269 113 159 535.2 0 159 114 125 535.2 0 125 115 119 535.2 0 119 116 108 535.2 0 108 117 141 535.2 0 141 118 150 535.2 0 150 119 135 535.2 0 135 120 127 535.2 0 127 121 122 535.2 0 122 122 124 535.2 0 124 123 101 535.2 0 101 124 128 535.2 0 128 125 136 535.2 0 136 126 130 535.2 0 130 127 137 535.2 0 137 128 144 535.2 0 144 129 133 535.2 0 133 130 202 535.2 0 202 131 179 535.2 0 179 132 210 535.2 0 210 133 152 535.2 0 152 134 158 535.2 0 158 135 133 535.2 0 133 136 165 535.2 0 165 137 127 535.2 0 127 138 248 535.2 0 248 139 238 535.2 0 238 140 176 535.2 0 176 141 140 535.2 0 140 142 292 535.2 0 292 143 188 535.2 0 188 144 215 535.2 0 215 145 183 535.2 0 183 146 229 535.2 0 229 147 153 535.2 0 153 148 132 535.2 0 132 149 155 535.2 0 155 150 119 535.2 0 119 151 127 535.2 0 127 152 140 535.2 0 140 153 144 535.2 0 144 154 137 535.2 0 137 155 152 535.2 0 152 156 136 535.2 0 136 157 120 535.2 0 120 158 145 535.2 0 145 159 142 535.2 0 142 160 116 535.2 0 116 161 124 535.2 0 124 162 134 535.2 0 134 163 132 535.2 0 132 164 129 535.2 0 129 165 122 535.2 0 122 166 111 535.2 0 111 167 117 535.2 0 117 168 142 535.2 0 142 169 109 535.2 0 109 170 112 535.2 0 112 171 130 535.2 0 130 172 106 535.2 0 106 173 124 535.2 0 124 174 123 535.2 0 123 175 121 535.2 0 121 176 128 535.2 0 128 177 115 535.2 0 115 178 118 535.2 0 118 179 124 535.2 0 124 180 196 535.2 0 196 181 126 535.2 0 126 182 114 535.2 0 114 183 120 535.2 0 120 184 126 535.2 0 126 185 116 535.2 0 116 186 120 535.2 0 120 187 118 535.2 0 118 188 116 535.2 0 116 189 113 535.2 0 113 190 144 535.2 0 144 191 163 535.2 0 163 192 264 535.2 0 264 193 217 535.2 0 217 194 194 535.2 0 194 195 122 535.2 0 122 196 147 535.2 0 147 197 474 535.2 0 474 198 438 535.2 0 438 199 642 535.2 0 642 200 134 535.2 0 134 201 110 535.2 0 110 202 84 535.2 0 84 203 92 535.2 0 92 204 100 535.2 0 100 205 136 535.2 0 136 206 130 535.2 0 130 207 105 535.2 0 105 208 79 535.2 0 79 209 114 535.2 0 114 210 119 535.2 0 119 211 110 535.2 0 110 212 110 535.2 0 110 213 110 535.2 0 110 214 111 535.2 0 111 215 101 535.2 0 101 216 110 535.2 0 110 217 126 535.2 0 126 218 130 535.2 0 130 219 94 535.2 0 94 220 96 535.2 0 96 221 101 535.2 0 101 222 143 535.2 0 143 223 174 535.2 0 174 224 255 535.2 0 255 225 435 535.2 0 435 226 175 535.2 0 175 227 147 535.2 0 147 228 106 535.2 0 106 229 105 535.2 0 105 230 115 535.2 0 115 231 133 535.2 0 133 232 116 535.2 0 116 233 161 535.2 0 161 234 157 535.2 0 157 235 156 535.2 0 156 236 157 535.2 0 157 237 126 535.2 0 126 238 234 535.2 0 234 239 149 535.2 0 149 240 225 535.2 0 225 241 331 535.2 0 331 242 263 535.2 0 263 243 157 535.2 0 157 244 138 535.2 0 138 245 170 535.2 0 170 246 200 535.2 0 200 247 202 535.2 0 202 248 131 535.2 0 131 249 125 535.2 0 125 250 100 535.2 0 100 251 121 535.2 0 121 252 128 535.2 0 128 253 107 535.2 0 107 254 159 535.2 0 159 255 190 535.2 0 190 256 226 535.2 0 226 257 176 535.2 0 176 258 173 535.2 0 173 259 133 535.2 0 133 260 198 535.2 0 198 261 193 535.2 0 193 262 241 535.2 0 241 263 195 535.2 0 195 264 177 535.2 0 177 265 569 535.2 0 569 266 897 535.2 0 897 267 1044 535.2 0 1044 268 287 535.2 0 287 269 93 535.2 0 93 270 119 535.2 0 119 271 130 535.2 0 130 272 192 535.2 0 192 273 173 535.2 0 173 274 217 535.2 0 217 275 182 535.2 0 182 276 132 535.2 0 132 277 143 535.2 0 143 278 125 535.2 0 125 279 163 535.2 0 163 280 133 535.2 0 133 281 165 535.2 0 165 282 138 535.2 0 138 283 172 535.2 0 172 284 173 535.2 0 173 285 254 535.2 0 254 286 231 535.2 0 231 287 180 535.2 0 180 288 279 535.2 0 279 289 189 535.2 0 189 290 228 535.2 0 228 291 274 535.2 0 274 292 377 535.2 0 377 293 334 535.2 0 334 294 361 535.2 0 361 295 390 535.2 0 390 296 448 535.2 0 448 297 537 535.2 0 537 298 2291 535.2 0 2291 299 4986 535.2 0 4986 300 11132 535.2 0 11132 301 5081 535.2 0 5081 Finished in 6.66 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 131,074 Reads with adapters: 45,323 (34.6%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 45,275 (34.5%) Total basepairs processed: 35,522,091 bp Total written (filtered): 12,308,235 bp (34.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 45323 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 6 1 18 52 0.0 3 0 0 28 13 11 19 748 0.0 3 0 633 60 42 13 20 43966 0.0 4 40169 2933 634 170 60 21 129 0.0 4 17 90 14 3 5 22 15 0.0 4 1 0 7 3 4 23 269 0.0 4 2 2 0 182 83 24 1 0.0 4 0 0 0 0 1 42 1 0.0 4 1 90 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 101 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 1 127 72 0.0 4 0 0 0 1 71 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 157 1 0.0 4 1 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 1 188 2 0.0 4 1 0 0 0 1 200 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 1 0.0 4 0 0 0 0 1 230 4 0.0 4 0 0 0 1 3 233 3 0.0 4 0 0 0 0 3 234 3 0.0 4 0 0 0 0 3 235 5 0.0 4 0 0 0 0 5 236 1 0.0 4 0 0 0 1 256 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 265 3 0.0 4 0 0 0 0 3 276 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.67 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 45,275 Reads with adapters: 11,024 (24.3%) Reads written (passing filters): 45,275 (100.0%) Total basepairs processed: 12,308,235 bp Total written (filtered): 12,179,850 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11024 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.5% C: 10.2% G: 83.8% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 39 707.4 0 39 4 11 176.9 0 11 5 2 44.2 0 2 7 3 2.8 0 3 8 3 0.7 0 3 9 2075 0.2 0 229 1846 10 6157 0.0 1 516 5641 11 2558 0.0 1 114 2444 12 39 0.0 1 1 38 13 24 0.0 1 0 24 14 4 0.0 1 0 4 40 1 0.0 1 1 62 1 0.0 1 1 70 1 0.0 1 0 1 103 1 0.0 1 0 1 104 1 0.0 1 1 105 2 0.0 1 0 2 106 4 0.0 1 1 3 107 3 0.0 1 2 1 139 1 0.0 1 1 147 2 0.0 1 2 150 29 0.0 1 25 4 151 3 0.0 1 3 152 2 0.0 1 1 1 208 48 0.0 1 39 9 209 8 0.0 1 7 1 210 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.