This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.30 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 85,881 (3.9%) Reads that were too short: 38,103 (1.7%) Reads written (passing filters): 47,778 (2.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 10,791,177 bp (1.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 85881 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 652 535.2 0 652 7 9663 535.2 0 9663 8 7341 535.2 0 7341 9 358 535.2 0 358 10 102 535.2 0 102 11 96 535.2 0 96 12 91 535.2 0 91 13 83 535.2 0 83 14 77 535.2 0 77 15 79 535.2 0 79 16 81 535.2 0 81 17 68 535.2 0 68 18 89 535.2 0 89 19 78 535.2 0 78 20 67 535.2 0 67 21 58 535.2 0 58 22 80 535.2 0 80 23 85 535.2 0 85 24 86 535.2 0 86 25 103 535.2 0 103 26 61 535.2 0 61 27 64 535.2 0 64 28 98 535.2 0 98 29 80 535.2 0 80 30 88 535.2 0 88 31 65 535.2 0 65 32 71 535.2 0 71 33 69 535.2 0 69 34 81 535.2 0 81 35 79 535.2 0 79 36 94 535.2 0 94 37 75 535.2 0 75 38 77 535.2 0 77 39 86 535.2 0 86 40 88 535.2 0 88 41 93 535.2 0 93 42 109 535.2 0 109 43 102 535.2 0 102 44 74 535.2 0 74 45 80 535.2 0 80 46 67 535.2 0 67 47 97 535.2 0 97 48 83 535.2 0 83 49 92 535.2 0 92 50 60 535.2 0 60 51 107 535.2 0 107 52 84 535.2 0 84 53 153 535.2 0 153 54 163 535.2 0 163 55 127 535.2 0 127 56 99 535.2 0 99 57 87 535.2 0 87 58 116 535.2 0 116 59 119 535.2 0 119 60 92 535.2 0 92 61 78 535.2 0 78 62 164 535.2 0 164 63 180 535.2 0 180 64 2485 535.2 0 2485 65 621 535.2 0 621 66 919 535.2 0 919 67 70 535.2 0 70 68 127 535.2 0 127 69 162 535.2 0 162 70 193 535.2 0 193 71 79 535.2 0 79 72 68 535.2 0 68 73 59 535.2 0 59 74 90 535.2 0 90 75 100 535.2 0 100 76 93 535.2 0 93 77 77 535.2 0 77 78 61 535.2 0 61 79 86 535.2 0 86 80 90 535.2 0 90 81 83 535.2 0 83 82 73 535.2 0 73 83 81 535.2 0 81 84 91 535.2 0 91 85 73 535.2 0 73 86 84 535.2 0 84 87 56 535.2 0 56 88 87 535.2 0 87 89 141 535.2 0 141 90 126 535.2 0 126 91 135 535.2 0 135 92 340 535.2 0 340 93 235 535.2 0 235 94 230 535.2 0 230 95 74 535.2 0 74 96 67 535.2 0 67 97 60 535.2 0 60 98 64 535.2 0 64 99 98 535.2 0 98 100 130 535.2 0 130 101 146 535.2 0 146 102 73 535.2 0 73 103 71 535.2 0 71 104 108 535.2 0 108 105 109 535.2 0 109 106 79 535.2 0 79 107 63 535.2 0 63 108 107 535.2 0 107 109 75 535.2 0 75 110 78 535.2 0 78 111 119 535.2 0 119 112 92 535.2 0 92 113 60 535.2 0 60 114 74 535.2 0 74 115 94 535.2 0 94 116 93 535.2 0 93 117 123 535.2 0 123 118 106 535.2 0 106 119 81 535.2 0 81 120 90 535.2 0 90 121 81 535.2 0 81 122 98 535.2 0 98 123 135 535.2 0 135 124 110 535.2 0 110 125 104 535.2 0 104 126 101 535.2 0 101 127 95 535.2 0 95 128 123 535.2 0 123 129 162 535.2 0 162 130 235 535.2 0 235 131 400 535.2 0 400 132 236 535.2 0 236 133 197 535.2 0 197 134 120 535.2 0 120 135 141 535.2 0 141 136 183 535.2 0 183 137 196 535.2 0 196 138 119 535.2 0 119 139 106 535.2 0 106 140 106 535.2 0 106 141 98 535.2 0 98 142 125 535.2 0 125 143 110 535.2 0 110 144 86 535.2 0 86 145 124 535.2 0 124 146 105 535.2 0 105 147 222 535.2 0 222 148 172 535.2 0 172 149 189 535.2 0 189 150 144 535.2 0 144 151 92 535.2 0 92 152 164 535.2 0 164 153 165 535.2 0 165 154 147 535.2 0 147 155 291 535.2 0 291 156 625 535.2 0 625 157 546 535.2 0 546 158 352 535.2 0 352 159 128 535.2 0 128 160 168 535.2 0 168 161 126 535.2 0 126 162 198 535.2 0 198 163 211 535.2 0 211 164 118 535.2 0 118 165 86 535.2 0 86 166 155 535.2 0 155 167 257 535.2 0 257 168 341 535.2 0 341 169 214 535.2 0 214 170 95 535.2 0 95 171 166 535.2 0 166 172 144 535.2 0 144 173 147 535.2 0 147 174 114 535.2 0 114 175 599 535.2 0 599 176 637 535.2 0 637 177 674 535.2 0 674 178 229 535.2 0 229 179 140 535.2 0 140 180 117 535.2 0 117 181 130 535.2 0 130 182 146 535.2 0 146 183 172 535.2 0 172 184 133 535.2 0 133 185 130 535.2 0 130 186 177 535.2 0 177 187 202 535.2 0 202 188 320 535.2 0 320 189 196 535.2 0 196 190 208 535.2 0 208 191 110 535.2 0 110 192 107 535.2 0 107 193 115 535.2 0 115 194 106 535.2 0 106 195 165 535.2 0 165 196 185 535.2 0 185 197 192 535.2 0 192 198 163 535.2 0 163 199 106 535.2 0 106 200 146 535.2 0 146 201 212 535.2 0 212 202 236 535.2 0 236 203 230 535.2 0 230 204 169 535.2 0 169 205 144 535.2 0 144 206 93 535.2 0 93 207 127 535.2 0 127 208 165 535.2 0 165 209 151 535.2 0 151 210 243 535.2 0 243 211 176 535.2 0 176 212 160 535.2 0 160 213 158 535.2 0 158 214 143 535.2 0 143 215 254 535.2 0 254 216 343 535.2 0 343 217 477 535.2 0 477 218 494 535.2 0 494 219 224 535.2 0 224 220 289 535.2 0 289 221 583 535.2 0 583 222 1087 535.2 0 1087 223 1234 535.2 0 1234 224 557 535.2 0 557 225 245 535.2 0 245 226 246 535.2 0 246 227 201 535.2 0 201 228 231 535.2 0 231 229 182 535.2 0 182 230 135 535.2 0 135 231 120 535.2 0 120 232 188 535.2 0 188 233 170 535.2 0 170 234 187 535.2 0 187 235 159 535.2 0 159 236 183 535.2 0 183 237 228 535.2 0 228 238 216 535.2 0 216 239 261 535.2 0 261 240 205 535.2 0 205 241 711 535.2 0 711 242 1037 535.2 0 1037 243 979 535.2 0 979 244 486 535.2 0 486 245 350 535.2 0 350 246 268 535.2 0 268 247 208 535.2 0 208 248 191 535.2 0 191 249 277 535.2 0 277 250 309 535.2 0 309 251 230 535.2 0 230 252 398 535.2 0 398 253 268 535.2 0 268 254 589 535.2 0 589 255 315 535.2 0 315 256 363 535.2 0 363 257 282 535.2 0 282 258 160 535.2 0 160 259 122 535.2 0 122 260 149 535.2 0 149 261 205 535.2 0 205 262 227 535.2 0 227 263 362 535.2 0 362 264 410 535.2 0 410 265 315 535.2 0 315 266 259 535.2 0 259 267 250 535.2 0 250 268 1133 535.2 0 1133 269 2800 535.2 0 2800 270 2892 535.2 0 2892 271 769 535.2 0 769 272 727 535.2 0 727 273 414 535.2 0 414 274 277 535.2 0 277 275 282 535.2 0 282 276 220 535.2 0 220 277 219 535.2 0 219 278 177 535.2 0 177 279 161 535.2 0 161 280 182 535.2 0 182 281 197 535.2 0 197 282 282 535.2 0 282 283 308 535.2 0 308 284 355 535.2 0 355 285 265 535.2 0 265 286 236 535.2 0 236 287 275 535.2 0 275 288 138 535.2 0 138 289 218 535.2 0 218 290 374 535.2 0 374 291 716 535.2 0 716 292 683 535.2 0 683 293 391 535.2 0 391 294 236 535.2 0 236 295 149 535.2 0 149 296 194 535.2 0 194 297 235 535.2 0 235 298 367 535.2 0 367 299 585 535.2 0 585 300 499 535.2 0 499 301 533 535.2 0 533 Finished in 2.26 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 47,778 Reads with adapters: 7,610 (15.9%) Reads that were too short: 24 (0.1%) Reads written (passing filters): 7,586 (15.9%) Total basepairs processed: 10,791,177 bp Total written (filtered): 2,067,843 bp (19.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7610 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 3 19 107 0.0 3 0 76 16 9 6 20 7234 0.0 4 6606 472 104 43 9 21 22 0.0 4 7 12 1 0 2 22 8 0.0 4 0 1 2 2 3 23 184 0.0 4 143 11 2 12 16 26 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 1 77 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 3 0.0 4 0 0 0 0 3 129 16 0.0 4 0 0 0 0 16 147 1 0.0 4 0 0 0 1 168 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 239 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 7,586 Reads with adapters: 1,719 (22.7%) Reads written (passing filters): 7,586 (100.0%) Total basepairs processed: 2,067,843 bp Total written (filtered): 2,048,182 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1719 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 9.7% G: 86.2% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 22 118.5 0 22 4 4 29.6 0 4 5 1 7.4 0 1 6 5 1.9 0 5 8 1 0.1 0 1 9 16 0.0 0 3 13 10 161 0.0 1 10 151 11 1489 0.0 1 64 1425 12 8 0.0 1 1 7 13 1 0.0 1 0 1 14 2 0.0 1 0 2 19 1 0.0 1 0 1 73 1 0.0 1 1 151 4 0.0 1 2 2 152 1 0.0 1 1 209 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.