This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.17 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 44,202 (2.0%) Reads that were too short: 13,100 (0.6%) Reads written (passing filters): 31,102 (1.4%) Total basepairs processed: 659,880,795 bp Total written (filtered): 7,802,059 bp (1.2%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 44202 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 531 535.2 0 531 7 8993 535.2 0 8993 8 8812 535.2 0 8812 9 124 535.2 0 124 10 51 535.2 0 51 11 42 535.2 0 42 12 48 535.2 0 48 13 52 535.2 0 52 14 46 535.2 0 46 15 43 535.2 0 43 16 45 535.2 0 45 17 49 535.2 0 49 18 50 535.2 0 50 19 40 535.2 0 40 20 48 535.2 0 48 21 43 535.2 0 43 22 39 535.2 0 39 23 41 535.2 0 41 24 38 535.2 0 38 25 43 535.2 0 43 26 39 535.2 0 39 27 41 535.2 0 41 28 66 535.2 0 66 29 57 535.2 0 57 30 55 535.2 0 55 31 49 535.2 0 49 32 134 535.2 0 134 33 63 535.2 0 63 34 39 535.2 0 39 35 50 535.2 0 50 36 38 535.2 0 38 37 52 535.2 0 52 38 56 535.2 0 56 39 50 535.2 0 50 40 50 535.2 0 50 41 48 535.2 0 48 42 47 535.2 0 47 43 53 535.2 0 53 44 62 535.2 0 62 45 39 535.2 0 39 46 48 535.2 0 48 47 74 535.2 0 74 48 43 535.2 0 43 49 54 535.2 0 54 50 39 535.2 0 39 51 39 535.2 0 39 52 41 535.2 0 41 53 53 535.2 0 53 54 72 535.2 0 72 55 53 535.2 0 53 56 28 535.2 0 28 57 44 535.2 0 44 58 40 535.2 0 40 59 48 535.2 0 48 60 35 535.2 0 35 61 30 535.2 0 30 62 50 535.2 0 50 63 76 535.2 0 76 64 570 535.2 0 570 65 132 535.2 0 132 66 218 535.2 0 218 67 28 535.2 0 28 68 83 535.2 0 83 69 87 535.2 0 87 70 49 535.2 0 49 71 31 535.2 0 31 72 38 535.2 0 38 73 34 535.2 0 34 74 40 535.2 0 40 75 36 535.2 0 36 76 43 535.2 0 43 77 33 535.2 0 33 78 32 535.2 0 32 79 43 535.2 0 43 80 55 535.2 0 55 81 64 535.2 0 64 82 30 535.2 0 30 83 52 535.2 0 52 84 49 535.2 0 49 85 49 535.2 0 49 86 50 535.2 0 50 87 37 535.2 0 37 88 37 535.2 0 37 89 70 535.2 0 70 90 73 535.2 0 73 91 74 535.2 0 74 92 238 535.2 0 238 93 155 535.2 0 155 94 117 535.2 0 117 95 46 535.2 0 46 96 38 535.2 0 38 97 35 535.2 0 35 98 33 535.2 0 33 99 41 535.2 0 41 100 44 535.2 0 44 101 43 535.2 0 43 102 49 535.2 0 49 103 50 535.2 0 50 104 57 535.2 0 57 105 56 535.2 0 56 106 57 535.2 0 57 107 39 535.2 0 39 108 67 535.2 0 67 109 48 535.2 0 48 110 46 535.2 0 46 111 64 535.2 0 64 112 49 535.2 0 49 113 40 535.2 0 40 114 45 535.2 0 45 115 56 535.2 0 56 116 58 535.2 0 58 117 61 535.2 0 61 118 71 535.2 0 71 119 41 535.2 0 41 120 70 535.2 0 70 121 60 535.2 0 60 122 65 535.2 0 65 123 61 535.2 0 61 124 49 535.2 0 49 125 52 535.2 0 52 126 61 535.2 0 61 127 48 535.2 0 48 128 47 535.2 0 47 129 64 535.2 0 64 130 72 535.2 0 72 131 88 535.2 0 88 132 85 535.2 0 85 133 64 535.2 0 64 134 39 535.2 0 39 135 53 535.2 0 53 136 58 535.2 0 58 137 70 535.2 0 70 138 57 535.2 0 57 139 67 535.2 0 67 140 73 535.2 0 73 141 86 535.2 0 86 142 86 535.2 0 86 143 50 535.2 0 50 144 42 535.2 0 42 145 45 535.2 0 45 146 46 535.2 0 46 147 76 535.2 0 76 148 55 535.2 0 55 149 89 535.2 0 89 150 96 535.2 0 96 151 58 535.2 0 58 152 66 535.2 0 66 153 103 535.2 0 103 154 62 535.2 0 62 155 107 535.2 0 107 156 160 535.2 0 160 157 133 535.2 0 133 158 108 535.2 0 108 159 52 535.2 0 52 160 65 535.2 0 65 161 62 535.2 0 62 162 65 535.2 0 65 163 65 535.2 0 65 164 48 535.2 0 48 165 44 535.2 0 44 166 66 535.2 0 66 167 79 535.2 0 79 168 85 535.2 0 85 169 74 535.2 0 74 170 48 535.2 0 48 171 74 535.2 0 74 172 73 535.2 0 73 173 77 535.2 0 77 174 54 535.2 0 54 175 220 535.2 0 220 176 194 535.2 0 194 177 191 535.2 0 191 178 77 535.2 0 77 179 62 535.2 0 62 180 71 535.2 0 71 181 62 535.2 0 62 182 48 535.2 0 48 183 73 535.2 0 73 184 74 535.2 0 74 185 52 535.2 0 52 186 97 535.2 0 97 187 63 535.2 0 63 188 100 535.2 0 100 189 87 535.2 0 87 190 97 535.2 0 97 191 70 535.2 0 70 192 70 535.2 0 70 193 68 535.2 0 68 194 52 535.2 0 52 195 50 535.2 0 50 196 72 535.2 0 72 197 65 535.2 0 65 198 63 535.2 0 63 199 78 535.2 0 78 200 75 535.2 0 75 201 55 535.2 0 55 202 64 535.2 0 64 203 80 535.2 0 80 204 74 535.2 0 74 205 81 535.2 0 81 206 78 535.2 0 78 207 71 535.2 0 71 208 80 535.2 0 80 209 65 535.2 0 65 210 72 535.2 0 72 211 77 535.2 0 77 212 82 535.2 0 82 213 88 535.2 0 88 214 73 535.2 0 73 215 92 535.2 0 92 216 93 535.2 0 93 217 119 535.2 0 119 218 110 535.2 0 110 219 94 535.2 0 94 220 85 535.2 0 85 221 138 535.2 0 138 222 193 535.2 0 193 223 191 535.2 0 191 224 156 535.2 0 156 225 108 535.2 0 108 226 132 535.2 0 132 227 97 535.2 0 97 228 105 535.2 0 105 229 86 535.2 0 86 230 77 535.2 0 77 231 55 535.2 0 55 232 65 535.2 0 65 233 65 535.2 0 65 234 117 535.2 0 117 235 79 535.2 0 79 236 73 535.2 0 73 237 102 535.2 0 102 238 72 535.2 0 72 239 90 535.2 0 90 240 82 535.2 0 82 241 222 535.2 0 222 242 219 535.2 0 219 243 241 535.2 0 241 244 143 535.2 0 143 245 90 535.2 0 90 246 98 535.2 0 98 247 100 535.2 0 100 248 95 535.2 0 95 249 90 535.2 0 90 250 96 535.2 0 96 251 96 535.2 0 96 252 131 535.2 0 131 253 131 535.2 0 131 254 169 535.2 0 169 255 98 535.2 0 98 256 94 535.2 0 94 257 90 535.2 0 90 258 73 535.2 0 73 259 68 535.2 0 68 260 66 535.2 0 66 261 60 535.2 0 60 262 99 535.2 0 99 263 106 535.2 0 106 264 100 535.2 0 100 265 96 535.2 0 96 266 107 535.2 0 107 267 100 535.2 0 100 268 469 535.2 0 469 269 1049 535.2 0 1049 270 1004 535.2 0 1004 271 167 535.2 0 167 272 148 535.2 0 148 273 154 535.2 0 154 274 95 535.2 0 95 275 114 535.2 0 114 276 111 535.2 0 111 277 98 535.2 0 98 278 104 535.2 0 104 279 77 535.2 0 77 280 83 535.2 0 83 281 75 535.2 0 75 282 83 535.2 0 83 283 107 535.2 0 107 284 125 535.2 0 125 285 92 535.2 0 92 286 83 535.2 0 83 287 105 535.2 0 105 288 96 535.2 0 96 289 78 535.2 0 78 290 119 535.2 0 119 291 159 535.2 0 159 292 193 535.2 0 193 293 127 535.2 0 127 294 87 535.2 0 87 295 95 535.2 0 95 296 95 535.2 0 95 297 123 535.2 0 123 298 184 535.2 0 184 299 224 535.2 0 224 300 216 535.2 0 216 301 222 535.2 0 222 Finished in 1.43 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 31,102 Reads with adapters: 9,005 (29.0%) Reads that were too short: 22 (0.1%) Reads written (passing filters): 8,983 (28.9%) Total basepairs processed: 7,802,059 bp Total written (filtered): 2,483,782 bp (31.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9005 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 5 0.0 3 0 0 5 16 63 0.0 3 0 63 17 7380 0.0 3 7227 151 1 1 18 34 0.0 3 5 27 1 1 19 1459 0.0 3 1434 22 3 20 26 0.0 3 23 1 0 2 21 1 0.0 3 1 31 1 0.0 3 0 0 0 1 36 1 0.0 3 1 48 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 128 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 1 141 1 0.0 3 0 0 0 1 152 1 0.0 3 0 1 153 1 0.0 3 1 157 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 8,983 Reads with adapters: 86 (1.0%) Reads written (passing filters): 8,983 (100.0%) Total basepairs processed: 2,483,782 bp Total written (filtered): 2,483,521 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 86 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.8% C: 3.5% G: 18.6% T: 29.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 83 140.4 0 83 4 3 35.1 0 3