This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.21 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 286,634 (13.1%) Reads that were too short: 139,351 (6.4%) Reads written (passing filters): 147,283 (6.7%) Total basepairs processed: 659,880,795 bp Total written (filtered): 40,741,087 bp (6.2%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 286634 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1194 535.2 0 1194 7 58152 535.2 0 58152 8 2385 535.2 0 2385 9 62205 535.2 0 62205 10 160 535.2 0 160 11 105 535.2 0 105 12 78 535.2 0 78 13 104 535.2 0 104 14 116 535.2 0 116 15 119 535.2 0 119 16 114 535.2 0 114 17 96 535.2 0 96 18 98 535.2 0 98 19 98 535.2 0 98 20 92 535.2 0 92 21 95 535.2 0 95 22 78 535.2 0 78 23 90 535.2 0 90 24 82 535.2 0 82 25 117 535.2 0 117 26 111 535.2 0 111 27 122 535.2 0 122 28 132 535.2 0 132 29 90 535.2 0 90 30 139 535.2 0 139 31 114 535.2 0 114 32 91 535.2 0 91 33 86 535.2 0 86 34 79 535.2 0 79 35 122 535.2 0 122 36 223 535.2 0 223 37 196 535.2 0 196 38 215 535.2 0 215 39 80 535.2 0 80 40 92 535.2 0 92 41 76 535.2 0 76 42 71 535.2 0 71 43 93 535.2 0 93 44 81 535.2 0 81 45 115 535.2 0 115 46 81 535.2 0 81 47 69 535.2 0 69 48 78 535.2 0 78 49 76 535.2 0 76 50 91 535.2 0 91 51 80 535.2 0 80 52 61 535.2 0 61 53 69 535.2 0 69 54 72 535.2 0 72 55 54 535.2 0 54 56 120 535.2 0 120 57 235 535.2 0 235 58 85 535.2 0 85 59 73 535.2 0 73 60 82 535.2 0 82 61 77 535.2 0 77 62 69 535.2 0 69 63 50 535.2 0 50 64 76 535.2 0 76 65 62 535.2 0 62 66 85 535.2 0 85 67 86 535.2 0 86 68 96 535.2 0 96 69 121 535.2 0 121 70 118 535.2 0 118 71 205 535.2 0 205 72 110 535.2 0 110 73 137 535.2 0 137 74 91 535.2 0 91 75 106 535.2 0 106 76 120 535.2 0 120 77 98 535.2 0 98 78 114 535.2 0 114 79 115 535.2 0 115 80 113 535.2 0 113 81 111 535.2 0 111 82 122 535.2 0 122 83 145 535.2 0 145 84 94 535.2 0 94 85 110 535.2 0 110 86 119 535.2 0 119 87 100 535.2 0 100 88 77 535.2 0 77 89 95 535.2 0 95 90 63 535.2 0 63 91 86 535.2 0 86 92 95 535.2 0 95 93 69 535.2 0 69 94 88 535.2 0 88 95 72 535.2 0 72 96 122 535.2 0 122 97 148 535.2 0 148 98 102 535.2 0 102 99 87 535.2 0 87 100 102 535.2 0 102 101 101 535.2 0 101 102 93 535.2 0 93 103 153 535.2 0 153 104 125 535.2 0 125 105 92 535.2 0 92 106 147 535.2 0 147 107 108 535.2 0 108 108 148 535.2 0 148 109 121 535.2 0 121 110 140 535.2 0 140 111 138 535.2 0 138 112 158 535.2 0 158 113 129 535.2 0 129 114 149 535.2 0 149 115 171 535.2 0 171 116 90 535.2 0 90 117 71 535.2 0 71 118 96 535.2 0 96 119 177 535.2 0 177 120 176 535.2 0 176 121 137 535.2 0 137 122 189 535.2 0 189 123 153 535.2 0 153 124 245 535.2 0 245 125 173 535.2 0 173 126 96 535.2 0 96 127 104 535.2 0 104 128 94 535.2 0 94 129 110 535.2 0 110 130 125 535.2 0 125 131 105 535.2 0 105 132 97 535.2 0 97 133 100 535.2 0 100 134 111 535.2 0 111 135 133 535.2 0 133 136 98 535.2 0 98 137 114 535.2 0 114 138 142 535.2 0 142 139 307 535.2 0 307 140 163 535.2 0 163 141 159 535.2 0 159 142 131 535.2 0 131 143 71 535.2 0 71 144 76 535.2 0 76 145 126 535.2 0 126 146 151 535.2 0 151 147 154 535.2 0 154 148 139 535.2 0 139 149 91 535.2 0 91 150 74 535.2 0 74 151 59 535.2 0 59 152 79 535.2 0 79 153 92 535.2 0 92 154 104 535.2 0 104 155 157 535.2 0 157 156 113 535.2 0 113 157 116 535.2 0 116 158 139 535.2 0 139 159 713 535.2 0 713 160 1007 535.2 0 1007 161 1135 535.2 0 1135 162 99 535.2 0 99 163 78 535.2 0 78 164 83 535.2 0 83 165 78 535.2 0 78 166 90 535.2 0 90 167 97 535.2 0 97 168 84 535.2 0 84 169 91 535.2 0 91 170 89 535.2 0 89 171 63 535.2 0 63 172 109 535.2 0 109 173 122 535.2 0 122 174 98 535.2 0 98 175 116 535.2 0 116 176 133 535.2 0 133 177 72 535.2 0 72 178 67 535.2 0 67 179 74 535.2 0 74 180 135 535.2 0 135 181 105 535.2 0 105 182 99 535.2 0 99 183 68 535.2 0 68 184 90 535.2 0 90 185 107 535.2 0 107 186 94 535.2 0 94 187 105 535.2 0 105 188 92 535.2 0 92 189 98 535.2 0 98 190 83 535.2 0 83 191 69 535.2 0 69 192 81 535.2 0 81 193 104 535.2 0 104 194 108 535.2 0 108 195 80 535.2 0 80 196 78 535.2 0 78 197 90 535.2 0 90 198 98 535.2 0 98 199 107 535.2 0 107 200 105 535.2 0 105 201 91 535.2 0 91 202 103 535.2 0 103 203 114 535.2 0 114 204 88 535.2 0 88 205 122 535.2 0 122 206 254 535.2 0 254 207 235 535.2 0 235 208 236 535.2 0 236 209 301 535.2 0 301 210 272 535.2 0 272 211 160 535.2 0 160 212 334 535.2 0 334 213 474 535.2 0 474 214 372 535.2 0 372 215 107 535.2 0 107 216 95 535.2 0 95 217 101 535.2 0 101 218 237 535.2 0 237 219 167 535.2 0 167 220 119 535.2 0 119 221 133 535.2 0 133 222 109 535.2 0 109 223 144 535.2 0 144 224 161 535.2 0 161 225 176 535.2 0 176 226 120 535.2 0 120 227 131 535.2 0 131 228 395 535.2 0 395 229 136 535.2 0 136 230 112 535.2 0 112 231 110 535.2 0 110 232 301 535.2 0 301 233 413 535.2 0 413 234 305 535.2 0 305 235 177 535.2 0 177 236 133 535.2 0 133 237 274 535.2 0 274 238 509 535.2 0 509 239 145 535.2 0 145 240 210 535.2 0 210 241 137 535.2 0 137 242 192 535.2 0 192 243 349 535.2 0 349 244 205 535.2 0 205 245 194 535.2 0 194 246 116 535.2 0 116 247 174 535.2 0 174 248 357 535.2 0 357 249 394 535.2 0 394 250 610 535.2 0 610 251 768 535.2 0 768 252 1027 535.2 0 1027 253 2063 535.2 0 2063 254 7618 535.2 0 7618 255 34271 535.2 0 34271 256 36219 535.2 0 36219 257 41835 535.2 0 41835 258 1170 535.2 0 1170 259 96 535.2 0 96 260 108 535.2 0 108 261 148 535.2 0 148 262 165 535.2 0 165 263 128 535.2 0 128 264 84 535.2 0 84 265 88 535.2 0 88 266 151 535.2 0 151 267 194 535.2 0 194 268 101 535.2 0 101 269 49 535.2 0 49 270 60 535.2 0 60 271 49 535.2 0 49 272 55 535.2 0 55 273 83 535.2 0 83 274 74 535.2 0 74 275 74 535.2 0 74 276 68 535.2 0 68 277 66 535.2 0 66 278 83 535.2 0 83 279 74 535.2 0 74 280 75 535.2 0 75 281 62 535.2 0 62 282 65 535.2 0 65 283 95 535.2 0 95 284 78 535.2 0 78 285 70 535.2 0 70 286 55 535.2 0 55 287 55 535.2 0 55 288 70 535.2 0 70 289 54 535.2 0 54 290 51 535.2 0 51 291 58 535.2 0 58 292 60 535.2 0 60 293 60 535.2 0 60 294 81 535.2 0 81 295 84 535.2 0 84 296 71 535.2 0 71 297 54 535.2 0 54 298 62 535.2 0 62 299 108 535.2 0 108 300 92 535.2 0 92 301 109 535.2 0 109 Finished in 7.58 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 147,283 Reads with adapters: 58,708 (39.9%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 58,662 (39.8%) Total basepairs processed: 40,741,087 bp Total written (filtered): 16,026,475 bp (39.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 58708 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 6 3 18 23 0.0 3 0 0 19 2 2 19 623 0.0 3 0 464 84 47 28 20 57225 0.0 4 52954 3289 707 203 72 21 161 0.0 4 28 118 11 1 3 22 21 0.0 4 5 4 10 1 1 23 167 0.0 4 3 1 1 115 47 24 3 0.0 4 0 1 0 0 2 26 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 1 44 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 89 4 0.0 4 0 0 0 0 4 93 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 121 1 0.0 4 1 124 1 0.0 4 0 0 0 0 1 126 8 0.0 4 0 0 0 0 8 127 399 0.0 4 0 0 0 0 399 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 163 1 0.0 4 0 0 0 1 183 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 215 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 6 0.0 4 0 0 0 1 5 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 253 2 0.0 4 0 0 0 1 1 256 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.03 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 58,662 Reads with adapters: 3,611 (6.2%) Reads written (passing filters): 58,662 (100.0%) Total basepairs processed: 16,026,475 bp Total written (filtered): 15,870,634 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3611 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.6% C: 23.9% G: 59.8% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 351 916.6 0 351 4 63 229.1 0 63 5 6 57.3 0 6 6 1 14.3 0 1 8 2 0.9 0 2 9 23 0.2 0 3 20 10 43 0.1 1 2 41 11 1622 0.0 1 74 1548 12 866 0.0 1 20 846 13 22 0.0 1 1 21 14 3 0.0 1 0 3 15 8 0.0 1 0 8 16 1 0.0 1 0 1 21 2 0.0 1 2 132 1 0.0 1 1 145 2 0.0 1 2 149 1 0.0 1 1 152 79 0.0 1 61 18 153 2 0.0 1 1 1 160 2 0.0 1 1 1 178 1 0.0 1 1 179 7 0.0 1 4 3 193 1 0.0 1 0 1 210 181 0.0 1 160 21 211 3 0.0 1 3 223 1 0.0 1 1 224 2 0.0 1 0 2 225 302 0.0 1 270 32 226 6 0.0 1 4 2 227 2 0.0 1 1 1 229 1 0.0 1 1 230 2 0.0 1 2 257 1 0.0 1 0 1 258 1 0.0 1 1