This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.39 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 108,052 (4.9%) Reads that were too short: 37,060 (1.7%) Reads written (passing filters): 70,992 (3.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 14,776,158 bp (2.2%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 108052 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 948 535.2 0 948 7 616 535.2 0 616 8 15639 535.2 0 15639 9 13088 535.2 0 13088 10 41 535.2 0 41 11 44 535.2 0 44 12 40 535.2 0 40 13 33 535.2 0 33 14 38 535.2 0 38 15 42 535.2 0 42 16 43 535.2 0 43 17 49 535.2 0 49 18 48 535.2 0 48 19 48 535.2 0 48 20 46 535.2 0 46 21 86 535.2 0 86 22 68 535.2 0 68 23 79 535.2 0 79 24 58 535.2 0 58 25 39 535.2 0 39 26 99 535.2 0 99 27 398 535.2 0 398 28 46 535.2 0 46 29 98 535.2 0 98 30 42 535.2 0 42 31 40 535.2 0 40 32 43 535.2 0 43 33 63 535.2 0 63 34 66 535.2 0 66 35 45 535.2 0 45 36 44 535.2 0 44 37 44 535.2 0 44 38 76 535.2 0 76 39 42 535.2 0 42 40 25 535.2 0 25 41 56 535.2 0 56 42 45 535.2 0 45 43 49 535.2 0 49 44 47 535.2 0 47 45 55 535.2 0 55 46 47 535.2 0 47 47 58 535.2 0 58 48 50 535.2 0 50 49 34 535.2 0 34 50 52 535.2 0 52 51 85 535.2 0 85 52 61 535.2 0 61 53 51 535.2 0 51 54 43 535.2 0 43 55 38 535.2 0 38 56 32 535.2 0 32 57 47 535.2 0 47 58 41 535.2 0 41 59 48 535.2 0 48 60 43 535.2 0 43 61 38 535.2 0 38 62 24 535.2 0 24 63 45 535.2 0 45 64 55 535.2 0 55 65 68 535.2 0 68 66 70 535.2 0 70 67 36 535.2 0 36 68 59 535.2 0 59 69 86 535.2 0 86 70 186 535.2 0 186 71 57 535.2 0 57 72 55 535.2 0 55 73 42 535.2 0 42 74 48 535.2 0 48 75 61 535.2 0 61 76 92 535.2 0 92 77 66 535.2 0 66 78 59 535.2 0 59 79 240 535.2 0 240 80 190 535.2 0 190 81 254 535.2 0 254 82 69 535.2 0 69 83 58 535.2 0 58 84 55 535.2 0 55 85 75 535.2 0 75 86 45 535.2 0 45 87 43 535.2 0 43 88 41 535.2 0 41 89 49 535.2 0 49 90 39 535.2 0 39 91 38 535.2 0 38 92 40 535.2 0 40 93 53 535.2 0 53 94 41 535.2 0 41 95 40 535.2 0 40 96 71 535.2 0 71 97 52 535.2 0 52 98 58 535.2 0 58 99 86 535.2 0 86 100 154 535.2 0 154 101 140 535.2 0 140 102 71 535.2 0 71 103 41 535.2 0 41 104 38 535.2 0 38 105 41 535.2 0 41 106 46 535.2 0 46 107 100 535.2 0 100 108 51 535.2 0 51 109 102 535.2 0 102 110 42 535.2 0 42 111 70 535.2 0 70 112 84 535.2 0 84 113 168 535.2 0 168 114 134 535.2 0 134 115 62 535.2 0 62 116 72 535.2 0 72 117 308 535.2 0 308 118 385 535.2 0 385 119 300 535.2 0 300 120 68 535.2 0 68 121 66 535.2 0 66 122 79 535.2 0 79 123 72 535.2 0 72 124 55 535.2 0 55 125 56 535.2 0 56 126 105 535.2 0 105 127 65 535.2 0 65 128 126 535.2 0 126 129 60 535.2 0 60 130 92 535.2 0 92 131 239 535.2 0 239 132 328 535.2 0 328 133 300 535.2 0 300 134 97 535.2 0 97 135 111 535.2 0 111 136 94 535.2 0 94 137 88 535.2 0 88 138 175 535.2 0 175 139 136 535.2 0 136 140 190 535.2 0 190 141 55 535.2 0 55 142 45 535.2 0 45 143 52 535.2 0 52 144 55 535.2 0 55 145 77 535.2 0 77 146 87 535.2 0 87 147 98 535.2 0 98 148 85 535.2 0 85 149 54 535.2 0 54 150 50 535.2 0 50 151 60 535.2 0 60 152 47 535.2 0 47 153 57 535.2 0 57 154 72 535.2 0 72 155 86 535.2 0 86 156 332 535.2 0 332 157 261 535.2 0 261 158 320 535.2 0 320 159 68 535.2 0 68 160 69 535.2 0 69 161 93 535.2 0 93 162 74 535.2 0 74 163 79 535.2 0 79 164 77 535.2 0 77 165 114 535.2 0 114 166 70 535.2 0 70 167 92 535.2 0 92 168 137 535.2 0 137 169 119 535.2 0 119 170 87 535.2 0 87 171 155 535.2 0 155 172 114 535.2 0 114 173 426 535.2 0 426 174 236 535.2 0 236 175 769 535.2 0 769 176 466 535.2 0 466 177 1074 535.2 0 1074 178 5103 535.2 0 5103 179 3003 535.2 0 3003 180 4873 535.2 0 4873 181 969 535.2 0 969 182 1876 535.2 0 1876 183 1369 535.2 0 1369 184 402 535.2 0 402 185 151 535.2 0 151 186 93 535.2 0 93 187 86 535.2 0 86 188 155 535.2 0 155 189 109 535.2 0 109 190 69 535.2 0 69 191 74 535.2 0 74 192 81 535.2 0 81 193 93 535.2 0 93 194 134 535.2 0 134 195 269 535.2 0 269 196 1123 535.2 0 1123 197 1356 535.2 0 1356 198 768 535.2 0 768 199 367 535.2 0 367 200 320 535.2 0 320 201 896 535.2 0 896 202 1987 535.2 0 1987 203 1683 535.2 0 1683 204 539 535.2 0 539 205 42 535.2 0 42 206 58 535.2 0 58 207 46 535.2 0 46 208 49 535.2 0 49 209 79 535.2 0 79 210 76 535.2 0 76 211 55 535.2 0 55 212 65 535.2 0 65 213 65 535.2 0 65 214 63 535.2 0 63 215 125 535.2 0 125 216 160 535.2 0 160 217 182 535.2 0 182 218 112 535.2 0 112 219 112 535.2 0 112 220 104 535.2 0 104 221 131 535.2 0 131 222 391 535.2 0 391 223 705 535.2 0 705 224 698 535.2 0 698 225 318 535.2 0 318 226 69 535.2 0 69 227 72 535.2 0 72 228 110 535.2 0 110 229 67 535.2 0 67 230 75 535.2 0 75 231 80 535.2 0 80 232 87 535.2 0 87 233 132 535.2 0 132 234 680 535.2 0 680 235 853 535.2 0 853 236 1341 535.2 0 1341 237 116 535.2 0 116 238 142 535.2 0 142 239 435 535.2 0 435 240 899 535.2 0 899 241 841 535.2 0 841 242 323 535.2 0 323 243 540 535.2 0 540 244 320 535.2 0 320 245 166 535.2 0 166 246 95 535.2 0 95 247 66 535.2 0 66 248 64 535.2 0 64 249 68 535.2 0 68 250 111 535.2 0 111 251 118 535.2 0 118 252 189 535.2 0 189 253 179 535.2 0 179 254 176 535.2 0 176 255 119 535.2 0 119 256 139 535.2 0 139 257 324 535.2 0 324 258 490 535.2 0 490 259 1304 535.2 0 1304 260 794 535.2 0 794 261 996 535.2 0 996 262 194 535.2 0 194 263 429 535.2 0 429 264 1635 535.2 0 1635 265 3614 535.2 0 3614 266 2538 535.2 0 2538 267 638 535.2 0 638 268 55 535.2 0 55 269 71 535.2 0 71 270 89 535.2 0 89 271 72 535.2 0 72 272 66 535.2 0 66 273 67 535.2 0 67 274 135 535.2 0 135 275 227 535.2 0 227 276 272 535.2 0 272 277 189 535.2 0 189 278 230 535.2 0 230 279 139 535.2 0 139 280 62 535.2 0 62 281 53 535.2 0 53 282 54 535.2 0 54 283 56 535.2 0 56 284 77 535.2 0 77 285 81 535.2 0 81 286 83 535.2 0 83 287 108 535.2 0 108 288 83 535.2 0 83 289 88 535.2 0 88 290 131 535.2 0 131 291 1107 535.2 0 1107 292 1310 535.2 0 1310 293 1378 535.2 0 1378 294 221 535.2 0 221 295 92 535.2 0 92 296 115 535.2 0 115 297 146 535.2 0 146 298 128 535.2 0 128 299 208 535.2 0 208 300 208 535.2 0 208 301 186 535.2 0 186 Finished in 3.21 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 70,992 Reads with adapters: 14,065 (19.8%) Reads that were too short: 42 (0.1%) Reads written (passing filters): 14,023 (19.8%) Total basepairs processed: 14,776,158 bp Total written (filtered): 3,803,469 bp (25.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14065 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 22 0.0 3 0 0 15 5 2 19 177 0.0 3 0 121 19 22 15 20 13562 0.0 4 12231 996 237 75 23 21 39 0.0 4 10 22 4 2 1 22 7 0.0 4 1 1 1 1 3 23 101 0.0 4 2 1 0 56 42 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 29 2 0.0 4 0 0 0 0 2 34 1 0.0 4 0 0 0 0 1 39 5 0.0 4 0 0 0 0 5 40 6 0.0 4 0 0 0 1 5 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 60 2 0.0 4 1 0 0 0 1 64 1 0.0 4 0 0 1 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 99 3 0.0 4 0 0 0 0 3 101 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 86 0.0 4 0 0 0 0 86 130 5 0.0 4 0 0 0 0 5 138 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 1 283 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.51 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 14,023 Reads with adapters: 3,211 (22.9%) Reads written (passing filters): 14,023 (100.0%) Total basepairs processed: 3,803,469 bp Total written (filtered): 3,770,384 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3211 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 10.4% G: 84.6% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 219.1 0 7 4 5 54.8 0 5 7 2 0.9 0 2 9 574 0.1 0 49 525 10 2125 0.0 1 189 1936 11 469 0.0 1 8 461 12 10 0.0 1 1 9 13 9 0.0 1 0 9 14 1 0.0 1 0 1 15 1 0.0 1 0 1 138 1 0.0 1 0 1 150 6 0.0 1 3 3 151 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.