This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 55.76 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 82,903 (3.8%) Reads that were too short: 22,898 (1.0%) Reads written (passing filters): 60,005 (2.7%) Total basepairs processed: 659,880,795 bp Total written (filtered): 13,345,039 bp (2.0%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 82903 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 896 535.2 0 896 7 461 535.2 0 461 8 14794 535.2 0 14794 9 15824 535.2 0 15824 10 47 535.2 0 47 11 28 535.2 0 28 12 12 535.2 0 12 13 40 535.2 0 40 14 20 535.2 0 20 15 24 535.2 0 24 16 19 535.2 0 19 17 24 535.2 0 24 18 36 535.2 0 36 19 21 535.2 0 21 20 25 535.2 0 25 21 45 535.2 0 45 22 52 535.2 0 52 23 36 535.2 0 36 24 22 535.2 0 22 25 23 535.2 0 23 26 42 535.2 0 42 27 37 535.2 0 37 28 32 535.2 0 32 29 51 535.2 0 51 30 25 535.2 0 25 31 22 535.2 0 22 32 19 535.2 0 19 33 50 535.2 0 50 34 42 535.2 0 42 35 46 535.2 0 46 36 28 535.2 0 28 37 32 535.2 0 32 38 39 535.2 0 39 39 45 535.2 0 45 40 38 535.2 0 38 41 32 535.2 0 32 42 44 535.2 0 44 43 32 535.2 0 32 44 36 535.2 0 36 45 28 535.2 0 28 46 23 535.2 0 23 47 43 535.2 0 43 48 45 535.2 0 45 49 36 535.2 0 36 50 42 535.2 0 42 51 27 535.2 0 27 52 39 535.2 0 39 53 35 535.2 0 35 54 40 535.2 0 40 55 29 535.2 0 29 56 33 535.2 0 33 57 44 535.2 0 44 58 39 535.2 0 39 59 38 535.2 0 38 60 38 535.2 0 38 61 28 535.2 0 28 62 35 535.2 0 35 63 23 535.2 0 23 64 32 535.2 0 32 65 38 535.2 0 38 66 34 535.2 0 34 67 29 535.2 0 29 68 32 535.2 0 32 69 76 535.2 0 76 70 184 535.2 0 184 71 48 535.2 0 48 72 26 535.2 0 26 73 24 535.2 0 24 74 34 535.2 0 34 75 23 535.2 0 23 76 40 535.2 0 40 77 43 535.2 0 43 78 51 535.2 0 51 79 254 535.2 0 254 80 119 535.2 0 119 81 174 535.2 0 174 82 20 535.2 0 20 83 35 535.2 0 35 84 27 535.2 0 27 85 59 535.2 0 59 86 29 535.2 0 29 87 24 535.2 0 24 88 27 535.2 0 27 89 24 535.2 0 24 90 26 535.2 0 26 91 21 535.2 0 21 92 18 535.2 0 18 93 35 535.2 0 35 94 25 535.2 0 25 95 33 535.2 0 33 96 44 535.2 0 44 97 31 535.2 0 31 98 38 535.2 0 38 99 31 535.2 0 31 100 59 535.2 0 59 101 47 535.2 0 47 102 24 535.2 0 24 103 28 535.2 0 28 104 30 535.2 0 30 105 26 535.2 0 26 106 34 535.2 0 34 107 70 535.2 0 70 108 33 535.2 0 33 109 101 535.2 0 101 110 40 535.2 0 40 111 40 535.2 0 40 112 40 535.2 0 40 113 59 535.2 0 59 114 33 535.2 0 33 115 26 535.2 0 26 116 42 535.2 0 42 117 109 535.2 0 109 118 89 535.2 0 89 119 87 535.2 0 87 120 40 535.2 0 40 121 26 535.2 0 26 122 30 535.2 0 30 123 32 535.2 0 32 124 40 535.2 0 40 125 35 535.2 0 35 126 71 535.2 0 71 127 33 535.2 0 33 128 73 535.2 0 73 129 36 535.2 0 36 130 25 535.2 0 25 131 74 535.2 0 74 132 94 535.2 0 94 133 60 535.2 0 60 134 48 535.2 0 48 135 38 535.2 0 38 136 40 535.2 0 40 137 60 535.2 0 60 138 76 535.2 0 76 139 49 535.2 0 49 140 155 535.2 0 155 141 29 535.2 0 29 142 24 535.2 0 24 143 39 535.2 0 39 144 34 535.2 0 34 145 33 535.2 0 33 146 52 535.2 0 52 147 46 535.2 0 46 148 40 535.2 0 40 149 38 535.2 0 38 150 34 535.2 0 34 151 24 535.2 0 24 152 33 535.2 0 33 153 47 535.2 0 47 154 53 535.2 0 53 155 62 535.2 0 62 156 317 535.2 0 317 157 265 535.2 0 265 158 291 535.2 0 291 159 38 535.2 0 38 160 43 535.2 0 43 161 49 535.2 0 49 162 59 535.2 0 59 163 45 535.2 0 45 164 61 535.2 0 61 165 97 535.2 0 97 166 68 535.2 0 68 167 55 535.2 0 55 168 89 535.2 0 89 169 71 535.2 0 71 170 73 535.2 0 73 171 132 535.2 0 132 172 91 535.2 0 91 173 397 535.2 0 397 174 170 535.2 0 170 175 749 535.2 0 749 176 280 535.2 0 280 177 642 535.2 0 642 178 4870 535.2 0 4870 179 2794 535.2 0 2794 180 5082 535.2 0 5082 181 563 535.2 0 563 182 1223 535.2 0 1223 183 317 535.2 0 317 184 226 535.2 0 226 185 85 535.2 0 85 186 56 535.2 0 56 187 52 535.2 0 52 188 119 535.2 0 119 189 41 535.2 0 41 190 38 535.2 0 38 191 33 535.2 0 33 192 43 535.2 0 43 193 49 535.2 0 49 194 73 535.2 0 73 195 106 535.2 0 106 196 431 535.2 0 431 197 474 535.2 0 474 198 318 535.2 0 318 199 114 535.2 0 114 200 224 535.2 0 224 201 238 535.2 0 238 202 571 535.2 0 571 203 496 535.2 0 496 204 167 535.2 0 167 205 32 535.2 0 32 206 38 535.2 0 38 207 30 535.2 0 30 208 41 535.2 0 41 209 38 535.2 0 38 210 49 535.2 0 49 211 42 535.2 0 42 212 42 535.2 0 42 213 36 535.2 0 36 214 31 535.2 0 31 215 76 535.2 0 76 216 74 535.2 0 74 217 92 535.2 0 92 218 46 535.2 0 46 219 49 535.2 0 49 220 55 535.2 0 55 221 83 535.2 0 83 222 149 535.2 0 149 223 217 535.2 0 217 224 263 535.2 0 263 225 153 535.2 0 153 226 45 535.2 0 45 227 48 535.2 0 48 228 51 535.2 0 51 229 56 535.2 0 56 230 52 535.2 0 52 231 57 535.2 0 57 232 70 535.2 0 70 233 109 535.2 0 109 234 876 535.2 0 876 235 1065 535.2 0 1065 236 1568 535.2 0 1568 237 78 535.2 0 78 238 96 535.2 0 96 239 257 535.2 0 257 240 672 535.2 0 672 241 521 535.2 0 521 242 124 535.2 0 124 243 147 535.2 0 147 244 124 535.2 0 124 245 72 535.2 0 72 246 50 535.2 0 50 247 46 535.2 0 46 248 29 535.2 0 29 249 61 535.2 0 61 250 68 535.2 0 68 251 74 535.2 0 74 252 97 535.2 0 97 253 84 535.2 0 84 254 78 535.2 0 78 255 71 535.2 0 71 256 73 535.2 0 73 257 111 535.2 0 111 258 193 535.2 0 193 259 1074 535.2 0 1074 260 631 535.2 0 631 261 931 535.2 0 931 262 129 535.2 0 129 263 420 535.2 0 420 264 1103 535.2 0 1103 265 2477 535.2 0 2477 266 1371 535.2 0 1371 267 402 535.2 0 402 268 35 535.2 0 35 269 31 535.2 0 31 270 28 535.2 0 28 271 34 535.2 0 34 272 34 535.2 0 34 273 57 535.2 0 57 274 81 535.2 0 81 275 62 535.2 0 62 276 79 535.2 0 79 277 52 535.2 0 52 278 83 535.2 0 83 279 65 535.2 0 65 280 33 535.2 0 33 281 45 535.2 0 45 282 51 535.2 0 51 283 50 535.2 0 50 284 50 535.2 0 50 285 50 535.2 0 50 286 52 535.2 0 52 287 61 535.2 0 61 288 45 535.2 0 45 289 56 535.2 0 56 290 79 535.2 0 79 291 690 535.2 0 690 292 858 535.2 0 858 293 731 535.2 0 731 294 124 535.2 0 124 295 79 535.2 0 79 296 75 535.2 0 75 297 128 535.2 0 128 298 92 535.2 0 92 299 122 535.2 0 122 300 144 535.2 0 144 301 111 535.2 0 111 Finished in 2.49 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 60,005 Reads with adapters: 15,229 (25.4%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 15,181 (25.3%) Total basepairs processed: 13,345,039 bp Total written (filtered): 4,184,811 bp (31.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15229 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 8 16 119 0.0 3 0 111 2 6 17 14331 0.0 3 14061 259 9 2 18 42 0.0 3 2 37 3 19 21 0.0 3 4 6 10 1 20 629 0.0 3 606 20 0 3 21 1 0.0 3 0 1 23 1 0.0 3 0 0 1 24 1 0.0 3 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 74 6 0.0 3 0 0 0 6 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 93 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 102 35 0.0 3 0 0 0 35 105 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 1 153 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 15,181 Reads with adapters: 191 (1.3%) Reads written (passing filters): 15,181 (100.0%) Total basepairs processed: 4,184,811 bp Total written (filtered): 4,184,207 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 191 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.3% C: 8.4% G: 16.2% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 161 237.2 0 161 4 29 59.3 0 29 5 1 14.8 0 1