This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.21 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 207,304 (9.5%) Reads that were too short: 141,345 (6.4%) Reads written (passing filters): 65,959 (3.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 17,407,474 bp (2.6%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 207304 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1162 535.2 0 1162 7 20590 535.2 0 20590 8 20562 535.2 0 20562 9 33 535.2 0 33 10 19 535.2 0 19 11 22 535.2 0 22 12 17 535.2 0 17 13 29 535.2 0 29 14 24 535.2 0 24 15 23 535.2 0 23 16 21 535.2 0 21 17 29 535.2 0 29 18 25 535.2 0 25 19 22 535.2 0 22 20 20 535.2 0 20 21 28 535.2 0 28 22 20 535.2 0 20 23 23 535.2 0 23 24 23 535.2 0 23 25 26 535.2 0 26 26 17 535.2 0 17 27 23 535.2 0 23 28 25 535.2 0 25 29 31 535.2 0 31 30 14 535.2 0 14 31 18 535.2 0 18 32 15 535.2 0 15 33 28 535.2 0 28 34 13 535.2 0 13 35 23 535.2 0 23 36 19 535.2 0 19 37 24 535.2 0 24 38 13 535.2 0 13 39 17 535.2 0 17 40 26 535.2 0 26 41 25 535.2 0 25 42 35 535.2 0 35 43 19 535.2 0 19 44 40 535.2 0 40 45 19 535.2 0 19 46 23 535.2 0 23 47 18 535.2 0 18 48 28 535.2 0 28 49 19 535.2 0 19 50 27 535.2 0 27 51 43 535.2 0 43 52 36 535.2 0 36 53 35 535.2 0 35 54 82 535.2 0 82 55 65 535.2 0 65 56 70 535.2 0 70 57 44 535.2 0 44 58 49 535.2 0 49 59 43 535.2 0 43 60 57 535.2 0 57 61 112 535.2 0 112 62 155 535.2 0 155 63 64 535.2 0 64 64 68 535.2 0 68 65 104 535.2 0 104 66 48 535.2 0 48 67 44 535.2 0 44 68 93 535.2 0 93 69 121 535.2 0 121 70 101 535.2 0 101 71 81 535.2 0 81 72 130 535.2 0 130 73 151 535.2 0 151 74 220 535.2 0 220 75 3702 535.2 0 3702 76 5802 535.2 0 5802 77 6084 535.2 0 6084 78 64 535.2 0 64 79 33 535.2 0 33 80 27 535.2 0 27 81 26 535.2 0 26 82 30 535.2 0 30 83 22 535.2 0 22 84 21 535.2 0 21 85 25 535.2 0 25 86 18 535.2 0 18 87 30 535.2 0 30 88 30 535.2 0 30 89 18 535.2 0 18 90 23 535.2 0 23 91 47 535.2 0 47 92 17 535.2 0 17 93 10 535.2 0 10 94 30 535.2 0 30 95 62 535.2 0 62 96 22 535.2 0 22 97 11 535.2 0 11 98 41 535.2 0 41 99 33 535.2 0 33 100 19 535.2 0 19 101 28 535.2 0 28 102 27 535.2 0 27 103 28 535.2 0 28 104 35 535.2 0 35 105 35 535.2 0 35 106 26 535.2 0 26 107 25 535.2 0 25 108 20 535.2 0 20 109 18 535.2 0 18 110 18 535.2 0 18 111 16 535.2 0 16 112 13 535.2 0 13 113 20 535.2 0 20 114 20 535.2 0 20 115 31 535.2 0 31 116 16 535.2 0 16 117 22 535.2 0 22 118 29 535.2 0 29 119 34 535.2 0 34 120 26 535.2 0 26 121 27 535.2 0 27 122 24 535.2 0 24 123 23 535.2 0 23 124 25 535.2 0 25 125 24 535.2 0 24 126 40 535.2 0 40 127 93 535.2 0 93 128 33 535.2 0 33 129 31 535.2 0 31 130 22 535.2 0 22 131 25 535.2 0 25 132 30 535.2 0 30 133 25 535.2 0 25 134 32 535.2 0 32 135 32 535.2 0 32 136 37 535.2 0 37 137 64 535.2 0 64 138 33 535.2 0 33 139 38 535.2 0 38 140 28 535.2 0 28 141 18 535.2 0 18 142 55 535.2 0 55 143 33 535.2 0 33 144 40 535.2 0 40 145 14 535.2 0 14 146 33 535.2 0 33 147 33 535.2 0 33 148 22 535.2 0 22 149 18 535.2 0 18 150 35 535.2 0 35 151 24 535.2 0 24 152 12 535.2 0 12 153 16 535.2 0 16 154 33 535.2 0 33 155 28 535.2 0 28 156 63 535.2 0 63 157 28 535.2 0 28 158 40 535.2 0 40 159 29 535.2 0 29 160 23 535.2 0 23 161 39 535.2 0 39 162 50 535.2 0 50 163 63 535.2 0 63 164 52 535.2 0 52 165 54 535.2 0 54 166 50 535.2 0 50 167 44 535.2 0 44 168 55 535.2 0 55 169 41 535.2 0 41 170 31 535.2 0 31 171 25 535.2 0 25 172 37 535.2 0 37 173 35 535.2 0 35 174 20 535.2 0 20 175 28 535.2 0 28 176 35 535.2 0 35 177 42 535.2 0 42 178 28 535.2 0 28 179 43 535.2 0 43 180 54 535.2 0 54 181 51 535.2 0 51 182 76 535.2 0 76 183 43 535.2 0 43 184 44 535.2 0 44 185 42 535.2 0 42 186 45 535.2 0 45 187 73 535.2 0 73 188 80 535.2 0 80 189 55 535.2 0 55 190 57 535.2 0 57 191 50 535.2 0 50 192 49 535.2 0 49 193 96 535.2 0 96 194 122 535.2 0 122 195 108 535.2 0 108 196 82 535.2 0 82 197 209 535.2 0 209 198 209 535.2 0 209 199 185 535.2 0 185 200 57 535.2 0 57 201 90 535.2 0 90 202 56 535.2 0 56 203 91 535.2 0 91 204 83 535.2 0 83 205 80 535.2 0 80 206 45 535.2 0 45 207 47 535.2 0 47 208 46 535.2 0 46 209 29 535.2 0 29 210 53 535.2 0 53 211 123 535.2 0 123 212 58 535.2 0 58 213 220 535.2 0 220 214 95 535.2 0 95 215 66 535.2 0 66 216 55 535.2 0 55 217 132 535.2 0 132 218 347 535.2 0 347 219 215 535.2 0 215 220 136 535.2 0 136 221 166 535.2 0 166 222 95 535.2 0 95 223 331 535.2 0 331 224 287 535.2 0 287 225 383 535.2 0 383 226 135 535.2 0 135 227 142 535.2 0 142 228 174 535.2 0 174 229 193 535.2 0 193 230 135 535.2 0 135 231 170 535.2 0 170 232 200 535.2 0 200 233 319 535.2 0 319 234 434 535.2 0 434 235 732 535.2 0 732 236 862 535.2 0 862 237 1370 535.2 0 1370 238 17629 535.2 0 17629 239 9429 535.2 0 9429 240 18508 535.2 0 18508 241 404 535.2 0 404 242 344 535.2 0 344 243 399 535.2 0 399 244 397 535.2 0 397 245 545 535.2 0 545 246 590 535.2 0 590 247 899 535.2 0 899 248 13174 535.2 0 13174 249 32519 535.2 0 32519 250 26005 535.2 0 26005 251 6060 535.2 0 6060 252 47 535.2 0 47 253 36 535.2 0 36 254 26 535.2 0 26 255 32 535.2 0 32 256 20 535.2 0 20 257 24 535.2 0 24 258 29 535.2 0 29 259 22 535.2 0 22 260 83 535.2 0 83 261 31 535.2 0 31 262 19 535.2 0 19 263 39 535.2 0 39 264 23 535.2 0 23 265 25 535.2 0 25 266 28 535.2 0 28 267 31 535.2 0 31 268 31 535.2 0 31 269 52 535.2 0 52 270 39 535.2 0 39 271 65 535.2 0 65 272 59 535.2 0 59 273 26 535.2 0 26 274 25 535.2 0 25 275 30 535.2 0 30 276 33 535.2 0 33 277 30 535.2 0 30 278 53 535.2 0 53 279 101 535.2 0 101 280 185 535.2 0 185 281 152 535.2 0 152 282 439 535.2 0 439 283 950 535.2 0 950 284 1017 535.2 0 1017 285 296 535.2 0 296 286 78 535.2 0 78 287 82 535.2 0 82 288 56 535.2 0 56 289 45 535.2 0 45 290 54 535.2 0 54 291 112 535.2 0 112 292 56 535.2 0 56 293 71 535.2 0 71 294 190 535.2 0 190 295 500 535.2 0 500 296 255 535.2 0 255 297 254 535.2 0 254 298 84 535.2 0 84 299 121 535.2 0 121 300 110 535.2 0 110 301 172 535.2 0 172 Finished in 3.00 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 65,959 Reads with adapters: 19,591 (29.7%) Reads that were too short: 68 (0.1%) Reads written (passing filters): 19,523 (29.6%) Total basepairs processed: 17,407,474 bp Total written (filtered): 5,376,316 bp (30.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19591 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 8 16 141 0.0 3 0 136 5 17 19094 0.0 3 18659 421 11 3 18 52 0.0 3 3 47 2 19 7 0.0 3 0 0 5 2 20 12 0.0 3 1 0 0 11 27 2 0.0 3 0 0 0 2 38 1 0.0 3 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 61 2 0.0 3 1 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 13 0.0 3 0 0 0 13 92 6 0.0 3 0 0 0 6 94 1 0.0 3 0 0 0 1 96 14 0.0 3 0 0 0 14 98 61 0.0 3 0 0 0 61 99 32 0.0 3 0 0 0 32 100 1 0.0 3 0 0 0 1 101 6 0.0 3 0 0 0 6 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 36 0.0 3 0 0 0 36 117 8 0.0 3 0 0 0 8 118 3 0.0 3 0 0 0 3 119 6 0.0 3 0 0 0 6 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 176 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 185 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 203 33 0.0 3 0 0 0 33 206 4 0.0 3 0 0 0 4 207 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 258 1 0.0 3 1 281 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 19,523 Reads with adapters: 28 (0.1%) Reads written (passing filters): 19,523 (100.0%) Total basepairs processed: 5,376,316 bp Total written (filtered): 5,376,231 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 28 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.7% C: 50.0% G: 3.6% T: 10.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 27 305.0 0 27 4 1 76.3 0 1