This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.52 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 116,442 (5.3%) Reads that were too short: 33,915 (1.5%) Reads written (passing filters): 82,527 (3.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 16,228,134 bp (2.5%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 116442 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 653 535.2 0 653 7 12807 535.2 0 12807 8 320 535.2 0 320 9 12885 535.2 0 12885 10 38 535.2 0 38 11 15 535.2 0 15 12 24 535.2 0 24 13 18 535.2 0 18 14 14 535.2 0 14 15 21 535.2 0 21 16 15 535.2 0 15 17 20 535.2 0 20 18 15 535.2 0 15 19 16 535.2 0 16 20 27 535.2 0 27 21 14 535.2 0 14 22 12 535.2 0 12 23 22 535.2 0 22 24 23 535.2 0 23 25 21 535.2 0 21 26 18 535.2 0 18 27 17 535.2 0 17 28 17 535.2 0 17 29 18 535.2 0 18 30 21 535.2 0 21 31 18 535.2 0 18 32 24 535.2 0 24 33 16 535.2 0 16 34 16 535.2 0 16 35 17 535.2 0 17 36 11 535.2 0 11 37 11 535.2 0 11 38 8 535.2 0 8 39 17 535.2 0 17 40 16 535.2 0 16 41 23 535.2 0 23 42 43 535.2 0 43 43 38 535.2 0 38 44 18 535.2 0 18 45 20 535.2 0 20 46 13 535.2 0 13 47 16 535.2 0 16 48 20 535.2 0 20 49 20 535.2 0 20 50 24 535.2 0 24 51 26 535.2 0 26 52 22 535.2 0 22 53 25 535.2 0 25 54 45 535.2 0 45 55 40 535.2 0 40 56 43 535.2 0 43 57 12 535.2 0 12 58 10 535.2 0 10 59 19 535.2 0 19 60 16 535.2 0 16 61 24 535.2 0 24 62 23 535.2 0 23 63 29 535.2 0 29 64 93 535.2 0 93 65 142 535.2 0 142 66 118 535.2 0 118 67 35 535.2 0 35 68 34 535.2 0 34 69 75 535.2 0 75 70 71 535.2 0 71 71 163 535.2 0 163 72 307 535.2 0 307 73 230 535.2 0 230 74 217 535.2 0 217 75 170 535.2 0 170 76 157 535.2 0 157 77 88 535.2 0 88 78 128 535.2 0 128 79 112 535.2 0 112 80 177 535.2 0 177 81 78 535.2 0 78 82 125 535.2 0 125 83 927 535.2 0 927 84 720 535.2 0 720 85 270 535.2 0 270 86 50 535.2 0 50 87 90 535.2 0 90 88 68 535.2 0 68 89 50 535.2 0 50 90 46 535.2 0 46 91 35 535.2 0 35 92 46 535.2 0 46 93 37 535.2 0 37 94 26 535.2 0 26 95 49 535.2 0 49 96 46 535.2 0 46 97 33 535.2 0 33 98 36 535.2 0 36 99 42 535.2 0 42 100 52 535.2 0 52 101 55 535.2 0 55 102 88 535.2 0 88 103 127 535.2 0 127 104 134 535.2 0 134 105 169 535.2 0 169 106 156 535.2 0 156 107 913 535.2 0 913 108 1757 535.2 0 1757 109 562 535.2 0 562 110 98 535.2 0 98 111 119 535.2 0 119 112 110 535.2 0 110 113 125 535.2 0 125 114 85 535.2 0 85 115 108 535.2 0 108 116 146 535.2 0 146 117 183 535.2 0 183 118 110 535.2 0 110 119 199 535.2 0 199 120 410 535.2 0 410 121 792 535.2 0 792 122 1179 535.2 0 1179 123 284 535.2 0 284 124 521 535.2 0 521 125 424 535.2 0 424 126 387 535.2 0 387 127 281 535.2 0 281 128 356 535.2 0 356 129 88 535.2 0 88 130 116 535.2 0 116 131 111 535.2 0 111 132 101 535.2 0 101 133 87 535.2 0 87 134 57 535.2 0 57 135 84 535.2 0 84 136 110 535.2 0 110 137 143 535.2 0 143 138 151 535.2 0 151 139 185 535.2 0 185 140 103 535.2 0 103 141 148 535.2 0 148 142 142 535.2 0 142 143 219 535.2 0 219 144 121 535.2 0 121 145 179 535.2 0 179 146 246 535.2 0 246 147 287 535.2 0 287 148 163 535.2 0 163 149 189 535.2 0 189 150 98 535.2 0 98 151 154 535.2 0 154 152 192 535.2 0 192 153 121 535.2 0 121 154 74 535.2 0 74 155 104 535.2 0 104 156 129 535.2 0 129 157 102 535.2 0 102 158 87 535.2 0 87 159 152 535.2 0 152 160 134 535.2 0 134 161 205 535.2 0 205 162 194 535.2 0 194 163 253 535.2 0 253 164 390 535.2 0 390 165 466 535.2 0 466 166 4178 535.2 0 4178 167 2844 535.2 0 2844 168 7775 535.2 0 7775 169 239 535.2 0 239 170 902 535.2 0 902 171 1103 535.2 0 1103 172 1080 535.2 0 1080 173 166 535.2 0 166 174 125 535.2 0 125 175 113 535.2 0 113 176 394 535.2 0 394 177 212 535.2 0 212 178 690 535.2 0 690 179 189 535.2 0 189 180 330 535.2 0 330 181 4150 535.2 0 4150 182 2649 535.2 0 2649 183 3746 535.2 0 3746 184 394 535.2 0 394 185 196 535.2 0 196 186 105 535.2 0 105 187 131 535.2 0 131 188 131 535.2 0 131 189 130 535.2 0 130 190 91 535.2 0 91 191 165 535.2 0 165 192 118 535.2 0 118 193 94 535.2 0 94 194 101 535.2 0 101 195 105 535.2 0 105 196 90 535.2 0 90 197 89 535.2 0 89 198 175 535.2 0 175 199 288 535.2 0 288 200 341 535.2 0 341 201 198 535.2 0 198 202 155 535.2 0 155 203 280 535.2 0 280 204 519 535.2 0 519 205 984 535.2 0 984 206 811 535.2 0 811 207 330 535.2 0 330 208 112 535.2 0 112 209 90 535.2 0 90 210 100 535.2 0 100 211 116 535.2 0 116 212 187 535.2 0 187 213 912 535.2 0 912 214 1181 535.2 0 1181 215 854 535.2 0 854 216 305 535.2 0 305 217 105 535.2 0 105 218 186 535.2 0 186 219 179 535.2 0 179 220 180 535.2 0 180 221 109 535.2 0 109 222 97 535.2 0 97 223 100 535.2 0 100 224 166 535.2 0 166 225 422 535.2 0 422 226 333 535.2 0 333 227 392 535.2 0 392 228 396 535.2 0 396 229 379 535.2 0 379 230 204 535.2 0 204 231 97 535.2 0 97 232 78 535.2 0 78 233 94 535.2 0 94 234 76 535.2 0 76 235 90 535.2 0 90 236 82 535.2 0 82 237 198 535.2 0 198 238 157 535.2 0 157 239 283 535.2 0 283 240 116 535.2 0 116 241 113 535.2 0 113 242 219 535.2 0 219 243 349 535.2 0 349 244 340 535.2 0 340 245 225 535.2 0 225 246 341 535.2 0 341 247 319 535.2 0 319 248 176 535.2 0 176 249 146 535.2 0 146 250 143 535.2 0 143 251 153 535.2 0 153 252 113 535.2 0 113 253 138 535.2 0 138 254 252 535.2 0 252 255 297 535.2 0 297 256 330 535.2 0 330 257 264 535.2 0 264 258 154 535.2 0 154 259 125 535.2 0 125 260 249 535.2 0 249 261 298 535.2 0 298 262 384 535.2 0 384 263 319 535.2 0 319 264 331 535.2 0 331 265 259 535.2 0 259 266 313 535.2 0 313 267 2919 535.2 0 2919 268 3822 535.2 0 3822 269 2984 535.2 0 2984 270 343 535.2 0 343 271 88 535.2 0 88 272 106 535.2 0 106 273 117 535.2 0 117 274 151 535.2 0 151 275 176 535.2 0 176 276 157 535.2 0 157 277 169 535.2 0 169 278 192 535.2 0 192 279 247 535.2 0 247 280 547 535.2 0 547 281 728 535.2 0 728 282 598 535.2 0 598 283 158 535.2 0 158 284 87 535.2 0 87 285 87 535.2 0 87 286 191 535.2 0 191 287 159 535.2 0 159 288 160 535.2 0 160 289 144 535.2 0 144 290 83 535.2 0 83 291 128 535.2 0 128 292 115 535.2 0 115 293 120 535.2 0 120 294 155 535.2 0 155 295 128 535.2 0 128 296 116 535.2 0 116 297 114 535.2 0 114 298 116 535.2 0 116 299 208 535.2 0 208 300 250 535.2 0 250 301 247 535.2 0 247 Finished in 3.42 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 82,527 Reads with adapters: 13,330 (16.2%) Reads that were too short: 72 (0.1%) Reads written (passing filters): 13,258 (16.1%) Total basepairs processed: 16,228,134 bp Total written (filtered): 3,619,251 bp (22.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13330 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 7 0.0 3 0 0 4 2 1 19 213 0.0 3 0 181 14 11 7 20 12827 0.0 4 11821 777 167 54 8 21 38 0.0 4 7 28 2 0 1 22 4 0.0 4 1 0 0 0 3 23 40 0.0 4 0 0 0 26 14 24 4 0.0 4 2 0 1 0 1 27 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 61 4 0.0 4 0 1 0 0 3 68 2 0.0 4 0 0 0 0 2 70 5 0.0 4 0 0 0 0 5 71 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 116 2 0.0 4 0 0 0 0 2 121 2 0.0 4 0 0 0 1 1 123 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 5 0.0 4 0 0 0 0 5 126 3 0.0 4 0 0 0 0 3 127 106 0.0 4 0 0 0 0 106 128 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 8 0.0 4 0 0 0 0 8 153 8 0.0 4 0 0 0 0 8 156 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 205 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 1 234 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 13,258 Reads with adapters: 965 (7.3%) Reads written (passing filters): 13,258 (100.0%) Total basepairs processed: 3,619,251 bp Total written (filtered): 3,545,362 bp (98.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 965 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.6% C: 39.9% G: 46.0% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 98 207.2 0 98 4 27 51.8 0 27 5 19 12.9 0 19 9 4 0.1 0 0 4 10 5 0.0 1 0 5 11 240 0.0 1 5 235 12 249 0.0 1 4 245 13 5 0.0 1 0 5 15 2 0.0 1 0 2 24 12 0.0 1 7 5 25 1 0.0 1 1 105 2 0.0 1 1 1 152 6 0.0 1 4 2 153 1 0.0 1 1 158 1 0.0 1 1 191 1 0.0 1 1 210 3 0.0 1 3 211 1 0.0 1 0 1 223 1 0.0 1 1 225 280 0.0 1 245 35 226 7 0.0 1 6 1