This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.19 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 108,796 (5.0%) Reads that were too short: 29,958 (1.4%) Reads written (passing filters): 78,838 (3.6%) Total basepairs processed: 659,880,795 bp Total written (filtered): 15,879,930 bp (2.4%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 108796 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 332 535.2 0 332 7 15121 535.2 0 15121 8 200 535.2 0 200 9 12458 535.2 0 12458 10 29 535.2 0 29 11 30 535.2 0 30 12 33 535.2 0 33 13 29 535.2 0 29 14 19 535.2 0 19 15 17 535.2 0 17 16 29 535.2 0 29 17 29 535.2 0 29 18 27 535.2 0 27 19 21 535.2 0 21 20 42 535.2 0 42 21 19 535.2 0 19 22 15 535.2 0 15 23 33 535.2 0 33 24 27 535.2 0 27 25 21 535.2 0 21 26 17 535.2 0 17 27 14 535.2 0 14 28 24 535.2 0 24 29 44 535.2 0 44 30 25 535.2 0 25 31 15 535.2 0 15 32 21 535.2 0 21 33 33 535.2 0 33 34 23 535.2 0 23 35 24 535.2 0 24 36 35 535.2 0 35 37 21 535.2 0 21 38 24 535.2 0 24 39 27 535.2 0 27 40 23 535.2 0 23 41 33 535.2 0 33 42 37 535.2 0 37 43 47 535.2 0 47 44 28 535.2 0 28 45 20 535.2 0 20 46 26 535.2 0 26 47 17 535.2 0 17 48 30 535.2 0 30 49 21 535.2 0 21 50 39 535.2 0 39 51 22 535.2 0 22 52 25 535.2 0 25 53 33 535.2 0 33 54 24 535.2 0 24 55 24 535.2 0 24 56 26 535.2 0 26 57 31 535.2 0 31 58 22 535.2 0 22 59 34 535.2 0 34 60 27 535.2 0 27 61 37 535.2 0 37 62 40 535.2 0 40 63 23 535.2 0 23 64 33 535.2 0 33 65 45 535.2 0 45 66 32 535.2 0 32 67 18 535.2 0 18 68 27 535.2 0 27 69 21 535.2 0 21 70 40 535.2 0 40 71 62 535.2 0 62 72 193 535.2 0 193 73 108 535.2 0 108 74 100 535.2 0 100 75 67 535.2 0 67 76 68 535.2 0 68 77 78 535.2 0 78 78 174 535.2 0 174 79 124 535.2 0 124 80 217 535.2 0 217 81 162 535.2 0 162 82 165 535.2 0 165 83 1029 535.2 0 1029 84 964 535.2 0 964 85 339 535.2 0 339 86 91 535.2 0 91 87 179 535.2 0 179 88 146 535.2 0 146 89 81 535.2 0 81 90 50 535.2 0 50 91 74 535.2 0 74 92 48 535.2 0 48 93 56 535.2 0 56 94 51 535.2 0 51 95 58 535.2 0 58 96 57 535.2 0 57 97 77 535.2 0 77 98 46 535.2 0 46 99 56 535.2 0 56 100 61 535.2 0 61 101 64 535.2 0 64 102 80 535.2 0 80 103 97 535.2 0 97 104 88 535.2 0 88 105 127 535.2 0 127 106 167 535.2 0 167 107 976 535.2 0 976 108 1730 535.2 0 1730 109 553 535.2 0 553 110 94 535.2 0 94 111 90 535.2 0 90 112 90 535.2 0 90 113 81 535.2 0 81 114 67 535.2 0 67 115 92 535.2 0 92 116 104 535.2 0 104 117 115 535.2 0 115 118 114 535.2 0 114 119 121 535.2 0 121 120 163 535.2 0 163 121 227 535.2 0 227 122 479 535.2 0 479 123 134 535.2 0 134 124 346 535.2 0 346 125 321 535.2 0 321 126 313 535.2 0 313 127 209 535.2 0 209 128 324 535.2 0 324 129 89 535.2 0 89 130 89 535.2 0 89 131 89 535.2 0 89 132 67 535.2 0 67 133 64 535.2 0 64 134 54 535.2 0 54 135 65 535.2 0 65 136 66 535.2 0 66 137 95 535.2 0 95 138 103 535.2 0 103 139 131 535.2 0 131 140 74 535.2 0 74 141 89 535.2 0 89 142 96 535.2 0 96 143 97 535.2 0 97 144 52 535.2 0 52 145 79 535.2 0 79 146 129 535.2 0 129 147 142 535.2 0 142 148 105 535.2 0 105 149 121 535.2 0 121 150 75 535.2 0 75 151 93 535.2 0 93 152 109 535.2 0 109 153 100 535.2 0 100 154 67 535.2 0 67 155 86 535.2 0 86 156 128 535.2 0 128 157 92 535.2 0 92 158 80 535.2 0 80 159 134 535.2 0 134 160 126 535.2 0 126 161 169 535.2 0 169 162 170 535.2 0 170 163 217 535.2 0 217 164 320 535.2 0 320 165 480 535.2 0 480 166 4540 535.2 0 4540 167 2861 535.2 0 2861 168 8616 535.2 0 8616 169 158 535.2 0 158 170 479 535.2 0 479 171 487 535.2 0 487 172 468 535.2 0 468 173 97 535.2 0 97 174 145 535.2 0 145 175 104 535.2 0 104 176 400 535.2 0 400 177 191 535.2 0 191 178 707 535.2 0 707 179 134 535.2 0 134 180 259 535.2 0 259 181 4382 535.2 0 4382 182 2508 535.2 0 2508 183 3725 535.2 0 3725 184 180 535.2 0 180 185 203 535.2 0 203 186 69 535.2 0 69 187 132 535.2 0 132 188 100 535.2 0 100 189 115 535.2 0 115 190 70 535.2 0 70 191 168 535.2 0 168 192 80 535.2 0 80 193 58 535.2 0 58 194 70 535.2 0 70 195 73 535.2 0 73 196 86 535.2 0 86 197 75 535.2 0 75 198 149 535.2 0 149 199 173 535.2 0 173 200 175 535.2 0 175 201 129 535.2 0 129 202 86 535.2 0 86 203 227 535.2 0 227 204 269 535.2 0 269 205 348 535.2 0 348 206 292 535.2 0 292 207 121 535.2 0 121 208 89 535.2 0 89 209 77 535.2 0 77 210 89 535.2 0 89 211 129 535.2 0 129 212 178 535.2 0 178 213 1208 535.2 0 1208 214 1397 535.2 0 1397 215 1118 535.2 0 1118 216 364 535.2 0 364 217 87 535.2 0 87 218 113 535.2 0 113 219 82 535.2 0 82 220 122 535.2 0 122 221 73 535.2 0 73 222 61 535.2 0 61 223 80 535.2 0 80 224 103 535.2 0 103 225 215 535.2 0 215 226 132 535.2 0 132 227 160 535.2 0 160 228 265 535.2 0 265 229 197 535.2 0 197 230 151 535.2 0 151 231 71 535.2 0 71 232 59 535.2 0 59 233 61 535.2 0 61 234 55 535.2 0 55 235 61 535.2 0 61 236 64 535.2 0 64 237 200 535.2 0 200 238 90 535.2 0 90 239 273 535.2 0 273 240 72 535.2 0 72 241 69 535.2 0 69 242 114 535.2 0 114 243 187 535.2 0 187 244 178 535.2 0 178 245 98 535.2 0 98 246 120 535.2 0 120 247 122 535.2 0 122 248 139 535.2 0 139 249 114 535.2 0 114 250 101 535.2 0 101 251 89 535.2 0 89 252 87 535.2 0 87 253 133 535.2 0 133 254 175 535.2 0 175 255 168 535.2 0 168 256 160 535.2 0 160 257 147 535.2 0 147 258 144 535.2 0 144 259 99 535.2 0 99 260 142 535.2 0 142 261 199 535.2 0 199 262 248 535.2 0 248 263 227 535.2 0 227 264 250 535.2 0 250 265 193 535.2 0 193 266 326 535.2 0 326 267 4107 535.2 0 4107 268 5484 535.2 0 5484 269 4196 535.2 0 4196 270 185 535.2 0 185 271 67 535.2 0 67 272 76 535.2 0 76 273 94 535.2 0 94 274 121 535.2 0 121 275 82 535.2 0 82 276 124 535.2 0 124 277 83 535.2 0 83 278 70 535.2 0 70 279 107 535.2 0 107 280 213 535.2 0 213 281 249 535.2 0 249 282 258 535.2 0 258 283 76 535.2 0 76 284 67 535.2 0 67 285 77 535.2 0 77 286 95 535.2 0 95 287 111 535.2 0 111 288 86 535.2 0 86 289 94 535.2 0 94 290 75 535.2 0 75 291 48 535.2 0 48 292 58 535.2 0 58 293 99 535.2 0 99 294 65 535.2 0 65 295 64 535.2 0 64 296 84 535.2 0 84 297 49 535.2 0 49 298 85 535.2 0 85 299 95 535.2 0 95 300 123 535.2 0 123 301 123 535.2 0 123 Finished in 3.00 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 78,838 Reads with adapters: 13,383 (17.0%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 13,353 (16.9%) Total basepairs processed: 15,879,930 bp Total written (filtered): 3,562,566 bp (22.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13383 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 7 0.0 3 0 0 7 16 76 0.0 3 0 74 2 17 12549 0.0 3 12314 230 5 18 36 0.0 3 1 31 4 19 13 0.0 3 0 0 11 2 20 4 0.0 3 0 0 0 4 21 15 0.0 3 0 0 1 14 23 1 0.0 3 0 0 0 1 24 9 0.0 3 0 0 2 7 25 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 1 40 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 2 0.0 3 1 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 1 66 602 0.0 3 0 0 5 597 67 1 0.0 3 0 0 1 70 1 0.0 3 0 0 0 1 71 4 0.0 3 0 0 0 4 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 3 0.0 3 0 0 0 3 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 239 1 0.0 3 1 243 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.46 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 13,353 Reads with adapters: 156 (1.2%) Reads written (passing filters): 13,353 (100.0%) Total basepairs processed: 3,562,566 bp Total written (filtered): 3,562,086 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 156 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.0% C: 9.0% G: 13.5% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 146 208.6 0 146 4 9 52.2 0 9 6 1 3.3 0 1