This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.43 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 159,116 (7.3%) Reads that were too short: 22,188 (1.0%) Reads written (passing filters): 136,928 (6.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 35,697,537 bp (5.4%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 159116 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 913 535.2 0 913 7 5093 535.2 0 5093 8 52159 535.2 0 52159 9 44146 535.2 0 44146 10 394 535.2 0 394 11 48 535.2 0 48 12 56 535.2 0 56 13 41 535.2 0 41 14 64 535.2 0 64 15 42 535.2 0 42 16 41 535.2 0 41 17 38 535.2 0 38 18 42 535.2 0 42 19 58 535.2 0 58 20 56 535.2 0 56 21 41 535.2 0 41 22 37 535.2 0 37 23 53 535.2 0 53 24 51 535.2 0 51 25 28 535.2 0 28 26 27 535.2 0 27 27 48 535.2 0 48 28 41 535.2 0 41 29 73 535.2 0 73 30 38 535.2 0 38 31 44 535.2 0 44 32 28 535.2 0 28 33 38 535.2 0 38 34 42 535.2 0 42 35 49 535.2 0 49 36 73 535.2 0 73 37 66 535.2 0 66 38 49 535.2 0 49 39 66 535.2 0 66 40 74 535.2 0 74 41 66 535.2 0 66 42 44 535.2 0 44 43 45 535.2 0 45 44 54 535.2 0 54 45 42 535.2 0 42 46 60 535.2 0 60 47 30 535.2 0 30 48 51 535.2 0 51 49 61 535.2 0 61 50 53 535.2 0 53 51 63 535.2 0 63 52 54 535.2 0 54 53 109 535.2 0 109 54 73 535.2 0 73 55 93 535.2 0 93 56 63 535.2 0 63 57 47 535.2 0 47 58 69 535.2 0 69 59 94 535.2 0 94 60 71 535.2 0 71 61 72 535.2 0 72 62 59 535.2 0 59 63 172 535.2 0 172 64 167 535.2 0 167 65 126 535.2 0 126 66 47 535.2 0 47 67 106 535.2 0 106 68 135 535.2 0 135 69 118 535.2 0 118 70 77 535.2 0 77 71 107 535.2 0 107 72 316 535.2 0 316 73 1415 535.2 0 1415 74 1370 535.2 0 1370 75 1049 535.2 0 1049 76 54 535.2 0 54 77 48 535.2 0 48 78 54 535.2 0 54 79 59 535.2 0 59 80 42 535.2 0 42 81 50 535.2 0 50 82 45 535.2 0 45 83 39 535.2 0 39 84 61 535.2 0 61 85 104 535.2 0 104 86 113 535.2 0 113 87 143 535.2 0 143 88 297 535.2 0 297 89 114 535.2 0 114 90 102 535.2 0 102 91 90 535.2 0 90 92 73 535.2 0 73 93 59 535.2 0 59 94 63 535.2 0 63 95 72 535.2 0 72 96 70 535.2 0 70 97 48 535.2 0 48 98 41 535.2 0 41 99 59 535.2 0 59 100 82 535.2 0 82 101 68 535.2 0 68 102 66 535.2 0 66 103 45 535.2 0 45 104 58 535.2 0 58 105 118 535.2 0 118 106 72 535.2 0 72 107 72 535.2 0 72 108 88 535.2 0 88 109 117 535.2 0 117 110 111 535.2 0 111 111 74 535.2 0 74 112 38 535.2 0 38 113 48 535.2 0 48 114 62 535.2 0 62 115 77 535.2 0 77 116 74 535.2 0 74 117 45 535.2 0 45 118 60 535.2 0 60 119 49 535.2 0 49 120 42 535.2 0 42 121 65 535.2 0 65 122 74 535.2 0 74 123 57 535.2 0 57 124 50 535.2 0 50 125 43 535.2 0 43 126 65 535.2 0 65 127 87 535.2 0 87 128 98 535.2 0 98 129 114 535.2 0 114 130 153 535.2 0 153 131 95 535.2 0 95 132 86 535.2 0 86 133 87 535.2 0 87 134 60 535.2 0 60 135 127 535.2 0 127 136 166 535.2 0 166 137 135 535.2 0 135 138 78 535.2 0 78 139 57 535.2 0 57 140 82 535.2 0 82 141 104 535.2 0 104 142 86 535.2 0 86 143 101 535.2 0 101 144 191 535.2 0 191 145 158 535.2 0 158 146 251 535.2 0 251 147 334 535.2 0 334 148 424 535.2 0 424 149 132 535.2 0 132 150 177 535.2 0 177 151 90 535.2 0 90 152 112 535.2 0 112 153 147 535.2 0 147 154 115 535.2 0 115 155 225 535.2 0 225 156 289 535.2 0 289 157 387 535.2 0 387 158 950 535.2 0 950 159 738 535.2 0 738 160 589 535.2 0 589 161 352 535.2 0 352 162 268 535.2 0 268 163 81 535.2 0 81 164 76 535.2 0 76 165 133 535.2 0 133 166 232 535.2 0 232 167 322 535.2 0 322 168 166 535.2 0 166 169 218 535.2 0 218 170 217 535.2 0 217 171 275 535.2 0 275 172 198 535.2 0 198 173 229 535.2 0 229 174 2756 535.2 0 2756 175 2017 535.2 0 2017 176 2147 535.2 0 2147 177 197 535.2 0 197 178 96 535.2 0 96 179 84 535.2 0 84 180 313 535.2 0 313 181 165 535.2 0 165 182 208 535.2 0 208 183 107 535.2 0 107 184 116 535.2 0 116 185 211 535.2 0 211 186 437 535.2 0 437 187 1529 535.2 0 1529 188 717 535.2 0 717 189 511 535.2 0 511 190 83 535.2 0 83 191 80 535.2 0 80 192 102 535.2 0 102 193 86 535.2 0 86 194 142 535.2 0 142 195 90 535.2 0 90 196 72 535.2 0 72 197 64 535.2 0 64 198 75 535.2 0 75 199 68 535.2 0 68 200 110 535.2 0 110 201 98 535.2 0 98 202 94 535.2 0 94 203 133 535.2 0 133 204 241 535.2 0 241 205 183 535.2 0 183 206 186 535.2 0 186 207 95 535.2 0 95 208 68 535.2 0 68 209 139 535.2 0 139 210 78 535.2 0 78 211 73 535.2 0 73 212 61 535.2 0 61 213 79 535.2 0 79 214 138 535.2 0 138 215 164 535.2 0 164 216 140 535.2 0 140 217 123 535.2 0 123 218 116 535.2 0 116 219 104 535.2 0 104 220 125 535.2 0 125 221 99 535.2 0 99 222 114 535.2 0 114 223 124 535.2 0 124 224 112 535.2 0 112 225 78 535.2 0 78 226 98 535.2 0 98 227 280 535.2 0 280 228 179 535.2 0 179 229 162 535.2 0 162 230 92 535.2 0 92 231 131 535.2 0 131 232 173 535.2 0 173 233 169 535.2 0 169 234 135 535.2 0 135 235 123 535.2 0 123 236 179 535.2 0 179 237 210 535.2 0 210 238 229 535.2 0 229 239 258 535.2 0 258 240 113 535.2 0 113 241 104 535.2 0 104 242 101 535.2 0 101 243 137 535.2 0 137 244 152 535.2 0 152 245 135 535.2 0 135 246 179 535.2 0 179 247 220 535.2 0 220 248 458 535.2 0 458 249 386 535.2 0 386 250 269 535.2 0 269 251 1232 535.2 0 1232 252 973 535.2 0 973 253 1518 535.2 0 1518 254 183 535.2 0 183 255 202 535.2 0 202 256 100 535.2 0 100 257 125 535.2 0 125 258 108 535.2 0 108 259 146 535.2 0 146 260 379 535.2 0 379 261 523 535.2 0 523 262 683 535.2 0 683 263 370 535.2 0 370 264 179 535.2 0 179 265 85 535.2 0 85 266 77 535.2 0 77 267 111 535.2 0 111 268 162 535.2 0 162 269 201 535.2 0 201 270 186 535.2 0 186 271 144 535.2 0 144 272 269 535.2 0 269 273 188 535.2 0 188 274 169 535.2 0 169 275 131 535.2 0 131 276 115 535.2 0 115 277 164 535.2 0 164 278 203 535.2 0 203 279 273 535.2 0 273 280 253 535.2 0 253 281 281 535.2 0 281 282 304 535.2 0 304 283 234 535.2 0 234 284 120 535.2 0 120 285 155 535.2 0 155 286 230 535.2 0 230 287 153 535.2 0 153 288 174 535.2 0 174 289 125 535.2 0 125 290 188 535.2 0 188 291 354 535.2 0 354 292 324 535.2 0 324 293 216 535.2 0 216 294 208 535.2 0 208 295 237 535.2 0 237 296 264 535.2 0 264 297 270 535.2 0 270 298 329 535.2 0 329 299 354 535.2 0 354 300 303 535.2 0 303 301 374 535.2 0 374 Finished in 6.63 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 136,928 Reads with adapters: 48,321 (35.3%) Reads that were too short: 76 (0.1%) Reads written (passing filters): 48,245 (35.2%) Total basepairs processed: 35,697,537 bp Total written (filtered): 13,072,413 bp (36.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 48321 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 4 6 18 58 0.0 3 0 0 34 13 11 19 661 0.0 3 0 539 49 45 28 20 46524 0.0 4 42440 3145 662 203 74 21 142 0.0 4 25 93 17 4 3 22 18 0.0 4 2 1 12 1 2 23 320 0.0 4 42 3 1 171 103 24 2 0.0 4 0 0 0 0 2 29 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 1 38 1 0.0 4 0 1 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 82 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 10 0.0 4 0 0 0 0 10 127 483 0.0 4 0 0 0 0 483 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 155 19 0.0 4 0 0 0 0 19 157 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 1 214 4 0.0 4 0 0 0 0 4 215 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 221 3 0.0 4 0 0 0 0 3 222 2 0.0 4 0 0 0 0 2 223 2 0.0 4 0 0 0 0 2 224 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 0 9 229 4 0.0 4 0 0 0 0 4 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 1 283 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.77 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 48,245 Reads with adapters: 11,410 (23.7%) Reads written (passing filters): 48,245 (100.0%) Total basepairs processed: 13,072,413 bp Total written (filtered): 12,943,294 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11410 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 10.7% G: 84.2% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 32 753.8 0 32 4 14 188.5 0 14 6 3 11.8 0 3 7 4 2.9 0 4 8 3 0.7 0 3 9 2094 0.2 0 236 1858 10 6438 0.0 1 515 5923 11 2647 0.0 1 123 2524 12 52 0.0 1 0 52 13 22 0.0 1 0 22 14 5 0.0 1 0 5 18 1 0.0 1 0 1 22 4 0.0 1 2 2 24 1 0.0 1 1 62 1 0.0 1 1 147 2 0.0 1 1 1 149 1 0.0 1 0 1 150 49 0.0 1 28 21 151 2 0.0 1 2 177 1 0.0 1 1 189 1 0.0 1 0 1 208 28 0.0 1 19 9 209 2 0.0 1 0 2 210 1 0.0 1 0 1 211 1 0.0 1 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.