This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.02 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 136,990 (6.2%) Reads that were too short: 9,548 (0.4%) Reads written (passing filters): 127,442 (5.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 35,370,079 bp (5.4%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 136990 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 887 535.2 0 887 7 3681 535.2 0 3681 8 48394 535.2 0 48394 9 56481 535.2 0 56481 10 105 535.2 0 105 11 29 535.2 0 29 12 43 535.2 0 43 13 32 535.2 0 32 14 55 535.2 0 55 15 38 535.2 0 38 16 34 535.2 0 34 17 34 535.2 0 34 18 35 535.2 0 35 19 44 535.2 0 44 20 43 535.2 0 43 21 46 535.2 0 46 22 29 535.2 0 29 23 41 535.2 0 41 24 34 535.2 0 34 25 34 535.2 0 34 26 29 535.2 0 29 27 39 535.2 0 39 28 42 535.2 0 42 29 30 535.2 0 30 30 35 535.2 0 35 31 42 535.2 0 42 32 42 535.2 0 42 33 29 535.2 0 29 34 31 535.2 0 31 35 28 535.2 0 28 36 34 535.2 0 34 37 40 535.2 0 40 38 40 535.2 0 40 39 43 535.2 0 43 40 39 535.2 0 39 41 40 535.2 0 40 42 41 535.2 0 41 43 32 535.2 0 32 44 43 535.2 0 43 45 29 535.2 0 29 46 36 535.2 0 36 47 37 535.2 0 37 48 35 535.2 0 35 49 28 535.2 0 28 50 35 535.2 0 35 51 26 535.2 0 26 52 43 535.2 0 43 53 37 535.2 0 37 54 371 535.2 0 371 55 535 535.2 0 535 56 55 535.2 0 55 57 33 535.2 0 33 58 46 535.2 0 46 59 44 535.2 0 44 60 35 535.2 0 35 61 49 535.2 0 49 62 41 535.2 0 41 63 26 535.2 0 26 64 47 535.2 0 47 65 37 535.2 0 37 66 40 535.2 0 40 67 54 535.2 0 54 68 62 535.2 0 62 69 67 535.2 0 67 70 47 535.2 0 47 71 83 535.2 0 83 72 420 535.2 0 420 73 1398 535.2 0 1398 74 1378 535.2 0 1378 75 1029 535.2 0 1029 76 42 535.2 0 42 77 43 535.2 0 43 78 37 535.2 0 37 79 56 535.2 0 56 80 34 535.2 0 34 81 18 535.2 0 18 82 29 535.2 0 29 83 27 535.2 0 27 84 37 535.2 0 37 85 49 535.2 0 49 86 66 535.2 0 66 87 59 535.2 0 59 88 75 535.2 0 75 89 62 535.2 0 62 90 60 535.2 0 60 91 46 535.2 0 46 92 44 535.2 0 44 93 45 535.2 0 45 94 43 535.2 0 43 95 43 535.2 0 43 96 33 535.2 0 33 97 32 535.2 0 32 98 35 535.2 0 35 99 47 535.2 0 47 100 52 535.2 0 52 101 40 535.2 0 40 102 28 535.2 0 28 103 29 535.2 0 29 104 34 535.2 0 34 105 79 535.2 0 79 106 65 535.2 0 65 107 29 535.2 0 29 108 57 535.2 0 57 109 64 535.2 0 64 110 47 535.2 0 47 111 35 535.2 0 35 112 35 535.2 0 35 113 33 535.2 0 33 114 27 535.2 0 27 115 46 535.2 0 46 116 51 535.2 0 51 117 36 535.2 0 36 118 40 535.2 0 40 119 48 535.2 0 48 120 32 535.2 0 32 121 43 535.2 0 43 122 39 535.2 0 39 123 32 535.2 0 32 124 25 535.2 0 25 125 38 535.2 0 38 126 32 535.2 0 32 127 44 535.2 0 44 128 49 535.2 0 49 129 62 535.2 0 62 130 45 535.2 0 45 131 38 535.2 0 38 132 28 535.2 0 28 133 57 535.2 0 57 134 44 535.2 0 44 135 48 535.2 0 48 136 127 535.2 0 127 137 46 535.2 0 46 138 57 535.2 0 57 139 30 535.2 0 30 140 41 535.2 0 41 141 43 535.2 0 43 142 45 535.2 0 45 143 38 535.2 0 38 144 64 535.2 0 64 145 79 535.2 0 79 146 93 535.2 0 93 147 127 535.2 0 127 148 252 535.2 0 252 149 68 535.2 0 68 150 176 535.2 0 176 151 41 535.2 0 41 152 46 535.2 0 46 153 46 535.2 0 46 154 44 535.2 0 44 155 79 535.2 0 79 156 70 535.2 0 70 157 87 535.2 0 87 158 312 535.2 0 312 159 217 535.2 0 217 160 166 535.2 0 166 161 161 535.2 0 161 162 55 535.2 0 55 163 33 535.2 0 33 164 45 535.2 0 45 165 45 535.2 0 45 166 64 535.2 0 64 167 49 535.2 0 49 168 51 535.2 0 51 169 117 535.2 0 117 170 99 535.2 0 99 171 172 535.2 0 172 172 87 535.2 0 87 173 97 535.2 0 97 174 965 535.2 0 965 175 574 535.2 0 574 176 678 535.2 0 678 177 56 535.2 0 56 178 49 535.2 0 49 179 38 535.2 0 38 180 218 535.2 0 218 181 67 535.2 0 67 182 61 535.2 0 61 183 39 535.2 0 39 184 63 535.2 0 63 185 56 535.2 0 56 186 144 535.2 0 144 187 411 535.2 0 411 188 201 535.2 0 201 189 177 535.2 0 177 190 53 535.2 0 53 191 59 535.2 0 59 192 35 535.2 0 35 193 51 535.2 0 51 194 70 535.2 0 70 195 34 535.2 0 34 196 45 535.2 0 45 197 42 535.2 0 42 198 34 535.2 0 34 199 48 535.2 0 48 200 50 535.2 0 50 201 41 535.2 0 41 202 49 535.2 0 49 203 50 535.2 0 50 204 86 535.2 0 86 205 74 535.2 0 74 206 62 535.2 0 62 207 50 535.2 0 50 208 30 535.2 0 30 209 36 535.2 0 36 210 29 535.2 0 29 211 39 535.2 0 39 212 28 535.2 0 28 213 34 535.2 0 34 214 48 535.2 0 48 215 53 535.2 0 53 216 47 535.2 0 47 217 43 535.2 0 43 218 43 535.2 0 43 219 50 535.2 0 50 220 33 535.2 0 33 221 44 535.2 0 44 222 45 535.2 0 45 223 45 535.2 0 45 224 41 535.2 0 41 225 32 535.2 0 32 226 41 535.2 0 41 227 146 535.2 0 146 228 54 535.2 0 54 229 84 535.2 0 84 230 75 535.2 0 75 231 60 535.2 0 60 232 70 535.2 0 70 233 68 535.2 0 68 234 49 535.2 0 49 235 49 535.2 0 49 236 57 535.2 0 57 237 61 535.2 0 61 238 92 535.2 0 92 239 84 535.2 0 84 240 43 535.2 0 43 241 53 535.2 0 53 242 41 535.2 0 41 243 59 535.2 0 59 244 59 535.2 0 59 245 47 535.2 0 47 246 67 535.2 0 67 247 73 535.2 0 73 248 134 535.2 0 134 249 90 535.2 0 90 250 114 535.2 0 114 251 915 535.2 0 915 252 768 535.2 0 768 253 982 535.2 0 982 254 45 535.2 0 45 255 73 535.2 0 73 256 44 535.2 0 44 257 37 535.2 0 37 258 58 535.2 0 58 259 69 535.2 0 69 260 103 535.2 0 103 261 119 535.2 0 119 262 140 535.2 0 140 263 105 535.2 0 105 264 62 535.2 0 62 265 40 535.2 0 40 266 38 535.2 0 38 267 42 535.2 0 42 268 59 535.2 0 59 269 62 535.2 0 62 270 64 535.2 0 64 271 56 535.2 0 56 272 69 535.2 0 69 273 64 535.2 0 64 274 87 535.2 0 87 275 90 535.2 0 90 276 67 535.2 0 67 277 79 535.2 0 79 278 114 535.2 0 114 279 139 535.2 0 139 280 106 535.2 0 106 281 84 535.2 0 84 282 100 535.2 0 100 283 85 535.2 0 85 284 51 535.2 0 51 285 106 535.2 0 106 286 79 535.2 0 79 287 67 535.2 0 67 288 74 535.2 0 74 289 60 535.2 0 60 290 69 535.2 0 69 291 81 535.2 0 81 292 89 535.2 0 89 293 56 535.2 0 56 294 88 535.2 0 88 295 170 535.2 0 170 296 86 535.2 0 86 297 115 535.2 0 115 298 108 535.2 0 108 299 111 535.2 0 111 300 146 535.2 0 146 301 166 535.2 0 166 Finished in 5.81 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 127,442 Reads with adapters: 49,150 (38.6%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 49,118 (38.5%) Total basepairs processed: 35,370,079 bp Total written (filtered): 13,557,164 bp (38.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49150 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 15 0.0 3 0 0 15 16 353 0.0 3 0 346 7 17 48568 0.0 3 47759 776 29 4 18 90 0.0 3 11 75 3 1 19 21 0.0 3 2 0 12 7 20 49 0.0 3 0 0 0 49 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 1 26 1 0.0 3 1 27 1 0.0 3 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 66 1 0.0 3 1 68 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 6 0.0 3 0 0 0 6 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 1 163 2 0.0 3 0 0 0 2 164 3 0.0 3 0 0 0 3 187 1 0.0 3 0 1 188 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 271 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.70 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 49,118 Reads with adapters: 345 (0.7%) Reads written (passing filters): 49,118 (100.0%) Total basepairs processed: 13,557,164 bp Total written (filtered): 13,556,045 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 345 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.8% C: 8.4% G: 21.7% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 268 767.5 0 268 4 76 191.9 0 76 11 1 0.0 1 0 1