This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.53 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 76,557 (3.5%) Reads that were too short: 8,819 (0.4%) Reads written (passing filters): 67,738 (3.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 16,368,444 bp (2.5%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 76557 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 765 535.2 0 765 7 20181 535.2 0 20181 8 23370 535.2 0 23370 9 30 535.2 0 30 10 25 535.2 0 25 11 61 535.2 0 61 12 32 535.2 0 32 13 74 535.2 0 74 14 75 535.2 0 75 15 90 535.2 0 90 16 32 535.2 0 32 17 19 535.2 0 19 18 11 535.2 0 11 19 20 535.2 0 20 20 11 535.2 0 11 21 23 535.2 0 23 22 8 535.2 0 8 23 20 535.2 0 20 24 11 535.2 0 11 25 136 535.2 0 136 26 204 535.2 0 204 27 15 535.2 0 15 28 22 535.2 0 22 29 11 535.2 0 11 30 13 535.2 0 13 31 18 535.2 0 18 32 22 535.2 0 22 33 20 535.2 0 20 34 13 535.2 0 13 35 9 535.2 0 9 36 10 535.2 0 10 37 12 535.2 0 12 38 8 535.2 0 8 39 9 535.2 0 9 40 10 535.2 0 10 41 20 535.2 0 20 42 25 535.2 0 25 43 17 535.2 0 17 44 26 535.2 0 26 45 15 535.2 0 15 46 10 535.2 0 10 47 28 535.2 0 28 48 18 535.2 0 18 49 18 535.2 0 18 50 19 535.2 0 19 51 17 535.2 0 17 52 12 535.2 0 12 53 24 535.2 0 24 54 19 535.2 0 19 55 16 535.2 0 16 56 23 535.2 0 23 57 26 535.2 0 26 58 21 535.2 0 21 59 18 535.2 0 18 60 53 535.2 0 53 61 40 535.2 0 40 62 41 535.2 0 41 63 18 535.2 0 18 64 19 535.2 0 19 65 25 535.2 0 25 66 18 535.2 0 18 67 12 535.2 0 12 68 15 535.2 0 15 69 21 535.2 0 21 70 21 535.2 0 21 71 19 535.2 0 19 72 15 535.2 0 15 73 18 535.2 0 18 74 25 535.2 0 25 75 25 535.2 0 25 76 19 535.2 0 19 77 32 535.2 0 32 78 20 535.2 0 20 79 33 535.2 0 33 80 43 535.2 0 43 81 52 535.2 0 52 82 229 535.2 0 229 83 203 535.2 0 203 84 877 535.2 0 877 85 20 535.2 0 20 86 40 535.2 0 40 87 47 535.2 0 47 88 33 535.2 0 33 89 50 535.2 0 50 90 36 535.2 0 36 91 19 535.2 0 19 92 14 535.2 0 14 93 22 535.2 0 22 94 24 535.2 0 24 95 43 535.2 0 43 96 33 535.2 0 33 97 24 535.2 0 24 98 40 535.2 0 40 99 23 535.2 0 23 100 37 535.2 0 37 101 39 535.2 0 39 102 42 535.2 0 42 103 36 535.2 0 36 104 66 535.2 0 66 105 37 535.2 0 37 106 38 535.2 0 38 107 77 535.2 0 77 108 76 535.2 0 76 109 41 535.2 0 41 110 36 535.2 0 36 111 65 535.2 0 65 112 97 535.2 0 97 113 48 535.2 0 48 114 35 535.2 0 35 115 28 535.2 0 28 116 33 535.2 0 33 117 35 535.2 0 35 118 39 535.2 0 39 119 48 535.2 0 48 120 73 535.2 0 73 121 40 535.2 0 40 122 64 535.2 0 64 123 83 535.2 0 83 124 67 535.2 0 67 125 105 535.2 0 105 126 31 535.2 0 31 127 37 535.2 0 37 128 45 535.2 0 45 129 48 535.2 0 48 130 38 535.2 0 38 131 42 535.2 0 42 132 117 535.2 0 117 133 114 535.2 0 114 134 184 535.2 0 184 135 38 535.2 0 38 136 37 535.2 0 37 137 60 535.2 0 60 138 64 535.2 0 64 139 50 535.2 0 50 140 80 535.2 0 80 141 50 535.2 0 50 142 59 535.2 0 59 143 39 535.2 0 39 144 50 535.2 0 50 145 100 535.2 0 100 146 39 535.2 0 39 147 209 535.2 0 209 148 40 535.2 0 40 149 35 535.2 0 35 150 32 535.2 0 32 151 31 535.2 0 31 152 37 535.2 0 37 153 32 535.2 0 32 154 35 535.2 0 35 155 44 535.2 0 44 156 52 535.2 0 52 157 62 535.2 0 62 158 50 535.2 0 50 159 64 535.2 0 64 160 277 535.2 0 277 161 219 535.2 0 219 162 324 535.2 0 324 163 68 535.2 0 68 164 40 535.2 0 40 165 46 535.2 0 46 166 27 535.2 0 27 167 50 535.2 0 50 168 99 535.2 0 99 169 114 535.2 0 114 170 150 535.2 0 150 171 30 535.2 0 30 172 47 535.2 0 47 173 62 535.2 0 62 174 53 535.2 0 53 175 66 535.2 0 66 176 49 535.2 0 49 177 60 535.2 0 60 178 38 535.2 0 38 179 64 535.2 0 64 180 115 535.2 0 115 181 169 535.2 0 169 182 229 535.2 0 229 183 163 535.2 0 163 184 137 535.2 0 137 185 185 535.2 0 185 186 188 535.2 0 188 187 2427 535.2 0 2427 188 2762 535.2 0 2762 189 7411 535.2 0 7411 190 58 535.2 0 58 191 41 535.2 0 41 192 43 535.2 0 43 193 40 535.2 0 40 194 40 535.2 0 40 195 26 535.2 0 26 196 39 535.2 0 39 197 43 535.2 0 43 198 51 535.2 0 51 199 40 535.2 0 40 200 49 535.2 0 49 201 46 535.2 0 46 202 46 535.2 0 46 203 52 535.2 0 52 204 72 535.2 0 72 205 70 535.2 0 70 206 134 535.2 0 134 207 94 535.2 0 94 208 78 535.2 0 78 209 44 535.2 0 44 210 249 535.2 0 249 211 192 535.2 0 192 212 243 535.2 0 243 213 89 535.2 0 89 214 56 535.2 0 56 215 43 535.2 0 43 216 39 535.2 0 39 217 54 535.2 0 54 218 44 535.2 0 44 219 80 535.2 0 80 220 206 535.2 0 206 221 104 535.2 0 104 222 52 535.2 0 52 223 40 535.2 0 40 224 34 535.2 0 34 225 59 535.2 0 59 226 49 535.2 0 49 227 52 535.2 0 52 228 59 535.2 0 59 229 73 535.2 0 73 230 288 535.2 0 288 231 516 535.2 0 516 232 368 535.2 0 368 233 53 535.2 0 53 234 28 535.2 0 28 235 32 535.2 0 32 236 32 535.2 0 32 237 33 535.2 0 33 238 24 535.2 0 24 239 57 535.2 0 57 240 56 535.2 0 56 241 56 535.2 0 56 242 43 535.2 0 43 243 36 535.2 0 36 244 37 535.2 0 37 245 34 535.2 0 34 246 38 535.2 0 38 247 23 535.2 0 23 248 31 535.2 0 31 249 34 535.2 0 34 250 34 535.2 0 34 251 35 535.2 0 35 252 50 535.2 0 50 253 56 535.2 0 56 254 78 535.2 0 78 255 77 535.2 0 77 256 45 535.2 0 45 257 48 535.2 0 48 258 50 535.2 0 50 259 34 535.2 0 34 260 46 535.2 0 46 261 67 535.2 0 67 262 34 535.2 0 34 263 97 535.2 0 97 264 66 535.2 0 66 265 53 535.2 0 53 266 50 535.2 0 50 267 39 535.2 0 39 268 74 535.2 0 74 269 73 535.2 0 73 270 44 535.2 0 44 271 39 535.2 0 39 272 58 535.2 0 58 273 79 535.2 0 79 274 80 535.2 0 80 275 192 535.2 0 192 276 202 535.2 0 202 277 243 535.2 0 243 278 111 535.2 0 111 279 154 535.2 0 154 280 147 535.2 0 147 281 83 535.2 0 83 282 55 535.2 0 55 283 76 535.2 0 76 284 67 535.2 0 67 285 80 535.2 0 80 286 40 535.2 0 40 287 63 535.2 0 63 288 165 535.2 0 165 289 274 535.2 0 274 290 157 535.2 0 157 291 178 535.2 0 178 292 36 535.2 0 36 293 57 535.2 0 57 294 78 535.2 0 78 295 124 535.2 0 124 296 78 535.2 0 78 297 106 535.2 0 106 298 40 535.2 0 40 299 63 535.2 0 63 300 171 535.2 0 171 301 147 535.2 0 147 Finished in 2.90 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 67,738 Reads with adapters: 20,098 (29.7%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 20,069 (29.6%) Total basepairs processed: 16,368,444 bp Total written (filtered): 5,551,560 bp (33.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20098 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 9 0.0 3 0 0 9 16 158 0.0 3 0 150 8 17 19762 0.0 3 19393 356 12 1 18 61 0.0 3 3 56 2 19 12 0.0 3 4 0 7 1 20 16 0.0 3 0 0 0 16 22 1 0.0 3 0 0 0 1 27 1 0.0 3 1 33 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 1 1 37 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 73 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 141 29 0.0 3 0 0 0 29 148 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 1 201 1 0.0 3 0 0 0 1 210 1 0.0 3 1 218 6 0.0 3 0 0 0 6 266 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 1 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.70 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 20,069 Reads with adapters: 58 (0.3%) Reads written (passing filters): 20,069 (100.0%) Total basepairs processed: 5,551,560 bp Total written (filtered): 5,551,373 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 58 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.9% C: 20.7% G: 24.1% T: 29.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 50 313.6 0 50 4 6 78.4 0 6 6 1 4.9 0 1 7 1 1.2 0 1