This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.79 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 130,479 (6.0%) Reads that were too short: 30,942 (1.4%) Reads written (passing filters): 99,537 (4.5%) Total basepairs processed: 659,880,795 bp Total written (filtered): 22,869,374 bp (3.5%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 130479 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1963 535.2 0 1963 7 22119 535.2 0 22119 8 962 535.2 0 962 9 28828 535.2 0 28828 10 74 535.2 0 74 11 29 535.2 0 29 12 33 535.2 0 33 13 64 535.2 0 64 14 29 535.2 0 29 15 28 535.2 0 28 16 24 535.2 0 24 17 29 535.2 0 29 18 33 535.2 0 33 19 38 535.2 0 38 20 82 535.2 0 82 21 23 535.2 0 23 22 24 535.2 0 24 23 37 535.2 0 37 24 26 535.2 0 26 25 22 535.2 0 22 26 30 535.2 0 30 27 31 535.2 0 31 28 43 535.2 0 43 29 33 535.2 0 33 30 34 535.2 0 34 31 43 535.2 0 43 32 33 535.2 0 33 33 29 535.2 0 29 34 36 535.2 0 36 35 59 535.2 0 59 36 46 535.2 0 46 37 40 535.2 0 40 38 133 535.2 0 133 39 73 535.2 0 73 40 104 535.2 0 104 41 39 535.2 0 39 42 35 535.2 0 35 43 39 535.2 0 39 44 20 535.2 0 20 45 41 535.2 0 41 46 35 535.2 0 35 47 39 535.2 0 39 48 22 535.2 0 22 49 35 535.2 0 35 50 42 535.2 0 42 51 27 535.2 0 27 52 22 535.2 0 22 53 311 535.2 0 311 54 152 535.2 0 152 55 161 535.2 0 161 56 60 535.2 0 60 57 76 535.2 0 76 58 67 535.2 0 67 59 34 535.2 0 34 60 62 535.2 0 62 61 38 535.2 0 38 62 36 535.2 0 36 63 63 535.2 0 63 64 59 535.2 0 59 65 62 535.2 0 62 66 624 535.2 0 624 67 192 535.2 0 192 68 466 535.2 0 466 69 24 535.2 0 24 70 70 535.2 0 70 71 75 535.2 0 75 72 90 535.2 0 90 73 58 535.2 0 58 74 55 535.2 0 55 75 30 535.2 0 30 76 34 535.2 0 34 77 26 535.2 0 26 78 32 535.2 0 32 79 28 535.2 0 28 80 22 535.2 0 22 81 32 535.2 0 32 82 43 535.2 0 43 83 51 535.2 0 51 84 36 535.2 0 36 85 42 535.2 0 42 86 60 535.2 0 60 87 46 535.2 0 46 88 40 535.2 0 40 89 68 535.2 0 68 90 71 535.2 0 71 91 88 535.2 0 88 92 48 535.2 0 48 93 69 535.2 0 69 94 69 535.2 0 69 95 85 535.2 0 85 96 40 535.2 0 40 97 36 535.2 0 36 98 50 535.2 0 50 99 30 535.2 0 30 100 29 535.2 0 29 101 73 535.2 0 73 102 72 535.2 0 72 103 169 535.2 0 169 104 151 535.2 0 151 105 273 535.2 0 273 106 119 535.2 0 119 107 111 535.2 0 111 108 78 535.2 0 78 109 90 535.2 0 90 110 55 535.2 0 55 111 155 535.2 0 155 112 149 535.2 0 149 113 239 535.2 0 239 114 81 535.2 0 81 115 105 535.2 0 105 116 50 535.2 0 50 117 60 535.2 0 60 118 56 535.2 0 56 119 48 535.2 0 48 120 86 535.2 0 86 121 70 535.2 0 70 122 82 535.2 0 82 123 114 535.2 0 114 124 113 535.2 0 113 125 1144 535.2 0 1144 126 777 535.2 0 777 127 1248 535.2 0 1248 128 74 535.2 0 74 129 112 535.2 0 112 130 89 535.2 0 89 131 57 535.2 0 57 132 94 535.2 0 94 133 194 535.2 0 194 134 115 535.2 0 115 135 123 535.2 0 123 136 62 535.2 0 62 137 89 535.2 0 89 138 98 535.2 0 98 139 114 535.2 0 114 140 136 535.2 0 136 141 90 535.2 0 90 142 113 535.2 0 113 143 118 535.2 0 118 144 263 535.2 0 263 145 514 535.2 0 514 146 407 535.2 0 407 147 293 535.2 0 293 148 159 535.2 0 159 149 202 535.2 0 202 150 837 535.2 0 837 151 528 535.2 0 528 152 964 535.2 0 964 153 173 535.2 0 173 154 363 535.2 0 363 155 582 535.2 0 582 156 1421 535.2 0 1421 157 1040 535.2 0 1040 158 578 535.2 0 578 159 810 535.2 0 810 160 4084 535.2 0 4084 161 3031 535.2 0 3031 162 3064 535.2 0 3064 163 184 535.2 0 184 164 194 535.2 0 194 165 136 535.2 0 136 166 201 535.2 0 201 167 123 535.2 0 123 168 117 535.2 0 117 169 128 535.2 0 128 170 138 535.2 0 138 171 172 535.2 0 172 172 87 535.2 0 87 173 164 535.2 0 164 174 166 535.2 0 166 175 577 535.2 0 577 176 379 535.2 0 379 177 435 535.2 0 435 178 144 535.2 0 144 179 338 535.2 0 338 180 1401 535.2 0 1401 181 3085 535.2 0 3085 182 2354 535.2 0 2354 183 744 535.2 0 744 184 108 535.2 0 108 185 169 535.2 0 169 186 168 535.2 0 168 187 129 535.2 0 129 188 129 535.2 0 129 189 76 535.2 0 76 190 82 535.2 0 82 191 90 535.2 0 90 192 65 535.2 0 65 193 61 535.2 0 61 194 83 535.2 0 83 195 92 535.2 0 92 196 77 535.2 0 77 197 80 535.2 0 80 198 86 535.2 0 86 199 152 535.2 0 152 200 117 535.2 0 117 201 77 535.2 0 77 202 75 535.2 0 75 203 82 535.2 0 82 204 98 535.2 0 98 205 81 535.2 0 81 206 72 535.2 0 72 207 87 535.2 0 87 208 125 535.2 0 125 209 167 535.2 0 167 210 167 535.2 0 167 211 418 535.2 0 418 212 1601 535.2 0 1601 213 1284 535.2 0 1284 214 1368 535.2 0 1368 215 142 535.2 0 142 216 109 535.2 0 109 217 139 535.2 0 139 218 110 535.2 0 110 219 172 535.2 0 172 220 128 535.2 0 128 221 137 535.2 0 137 222 141 535.2 0 141 223 165 535.2 0 165 224 279 535.2 0 279 225 281 535.2 0 281 226 182 535.2 0 182 227 111 535.2 0 111 228 122 535.2 0 122 229 83 535.2 0 83 230 124 535.2 0 124 231 91 535.2 0 91 232 97 535.2 0 97 233 126 535.2 0 126 234 207 535.2 0 207 235 159 535.2 0 159 236 125 535.2 0 125 237 154 535.2 0 154 238 284 535.2 0 284 239 254 535.2 0 254 240 268 535.2 0 268 241 230 535.2 0 230 242 167 535.2 0 167 243 285 535.2 0 285 244 196 535.2 0 196 245 219 535.2 0 219 246 260 535.2 0 260 247 309 535.2 0 309 248 336 535.2 0 336 249 165 535.2 0 165 250 134 535.2 0 134 251 110 535.2 0 110 252 206 535.2 0 206 253 107 535.2 0 107 254 191 535.2 0 191 255 297 535.2 0 297 256 1266 535.2 0 1266 257 1011 535.2 0 1011 258 1168 535.2 0 1168 259 347 535.2 0 347 260 202 535.2 0 202 261 174 535.2 0 174 262 193 535.2 0 193 263 189 535.2 0 189 264 138 535.2 0 138 265 129 535.2 0 129 266 481 535.2 0 481 267 617 535.2 0 617 268 1102 535.2 0 1102 269 381 535.2 0 381 270 208 535.2 0 208 271 265 535.2 0 265 272 630 535.2 0 630 273 971 535.2 0 971 274 1119 535.2 0 1119 275 785 535.2 0 785 276 189 535.2 0 189 277 178 535.2 0 178 278 224 535.2 0 224 279 292 535.2 0 292 280 483 535.2 0 483 281 415 535.2 0 415 282 363 535.2 0 363 283 205 535.2 0 205 284 226 535.2 0 226 285 202 535.2 0 202 286 202 535.2 0 202 287 403 535.2 0 403 288 401 535.2 0 401 289 486 535.2 0 486 290 140 535.2 0 140 291 175 535.2 0 175 292 191 535.2 0 191 293 311 535.2 0 311 294 195 535.2 0 195 295 207 535.2 0 207 296 125 535.2 0 125 297 140 535.2 0 140 298 122 535.2 0 122 299 148 535.2 0 148 300 161 535.2 0 161 301 355 535.2 0 355 Finished in 4.55 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 99,537 Reads with adapters: 24,012 (24.1%) Reads that were too short: 60 (0.1%) Reads written (passing filters): 23,952 (24.1%) Total basepairs processed: 22,869,374 bp Total written (filtered): 6,519,872 bp (28.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24012 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 3 0.0 3 0 0 0 1 2 18 27 0.0 3 0 0 17 3 7 19 445 0.0 3 0 369 46 22 8 20 22731 0.0 4 20929 1400 281 90 31 21 268 0.0 4 202 50 7 6 3 22 7 0.0 4 1 1 4 0 1 23 63 0.0 4 0 0 0 42 21 24 2 0.0 4 1 0 0 0 1 28 2 0.0 4 0 0 0 0 2 29 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 60 4 0.0 4 0 0 0 0 4 61 7 0.0 4 0 0 0 3 4 62 4 0.0 4 0 0 0 0 4 66 1 0.0 4 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 1 0 0 1 109 5 0.0 4 0 0 0 0 5 110 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 3 0.0 4 0 0 0 0 3 127 383 0.0 4 0 0 0 0 383 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 5 0.0 4 0 0 0 0 5 230 1 0.0 4 0 0 0 0 1 232 2 0.0 4 0 0 0 0 2 234 4 0.0 4 0 0 0 0 4 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 257 3 0.0 4 0 0 0 0 3 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 284 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.81 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 23,952 Reads with adapters: 1,102 (4.6%) Reads written (passing filters): 23,952 (100.0%) Total basepairs processed: 6,519,872 bp Total written (filtered): 6,504,660 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1102 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.5% C: 19.2% G: 67.6% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 154 374.2 0 154 4 31 93.6 0 31 5 1 23.4 0 1 6 1 5.8 0 1 9 7 0.1 0 3 4 10 40 0.0 1 1 39 11 442 0.0 1 29 413 12 371 0.0 1 8 363 13 23 0.0 1 0 23 14 1 0.0 1 0 1 15 4 0.0 1 0 4 20 1 0.0 1 0 1 152 16 0.0 1 10 6 154 1 0.0 1 1 171 1 0.0 1 1 180 1 0.0 1 1 195 1 0.0 1 1 209 1 0.0 1 1 210 3 0.0 1 3 212 1 0.0 1 1 262 1 0.0 1 1