This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.00 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 99,491 (4.5%) Reads that were too short: 16,214 (0.7%) Reads written (passing filters): 83,277 (3.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 20,934,404 bp (3.2%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 99491 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1666 535.2 0 1666 7 30494 535.2 0 30494 8 733 535.2 0 733 9 24328 535.2 0 24328 10 44 535.2 0 44 11 20 535.2 0 20 12 34 535.2 0 34 13 36 535.2 0 36 14 27 535.2 0 27 15 28 535.2 0 28 16 26 535.2 0 26 17 20 535.2 0 20 18 19 535.2 0 19 19 29 535.2 0 29 20 40 535.2 0 40 21 30 535.2 0 30 22 26 535.2 0 26 23 21 535.2 0 21 24 18 535.2 0 18 25 20 535.2 0 20 26 15 535.2 0 15 27 20 535.2 0 20 28 38 535.2 0 38 29 24 535.2 0 24 30 28 535.2 0 28 31 18 535.2 0 18 32 15 535.2 0 15 33 16 535.2 0 16 34 35 535.2 0 35 35 550 535.2 0 550 36 32 535.2 0 32 37 29 535.2 0 29 38 98 535.2 0 98 39 42 535.2 0 42 40 84 535.2 0 84 41 35 535.2 0 35 42 32 535.2 0 32 43 67 535.2 0 67 44 73 535.2 0 73 45 26 535.2 0 26 46 16 535.2 0 16 47 15 535.2 0 15 48 12 535.2 0 12 49 17 535.2 0 17 50 39 535.2 0 39 51 15 535.2 0 15 52 17 535.2 0 17 53 119 535.2 0 119 54 94 535.2 0 94 55 83 535.2 0 83 56 22 535.2 0 22 57 29 535.2 0 29 58 22 535.2 0 22 59 24 535.2 0 24 60 31 535.2 0 31 61 26 535.2 0 26 62 14 535.2 0 14 63 26 535.2 0 26 64 26 535.2 0 26 65 24 535.2 0 24 66 120 535.2 0 120 67 35 535.2 0 35 68 46 535.2 0 46 69 15 535.2 0 15 70 57 535.2 0 57 71 63 535.2 0 63 72 28 535.2 0 28 73 16 535.2 0 16 74 22 535.2 0 22 75 26 535.2 0 26 76 12 535.2 0 12 77 22 535.2 0 22 78 20 535.2 0 20 79 13 535.2 0 13 80 20 535.2 0 20 81 29 535.2 0 29 82 31 535.2 0 31 83 53 535.2 0 53 84 28 535.2 0 28 85 34 535.2 0 34 86 31 535.2 0 31 87 46 535.2 0 46 88 42 535.2 0 42 89 30 535.2 0 30 90 28 535.2 0 28 91 65 535.2 0 65 92 29 535.2 0 29 93 33 535.2 0 33 94 35 535.2 0 35 95 55 535.2 0 55 96 32 535.2 0 32 97 31 535.2 0 31 98 18 535.2 0 18 99 38 535.2 0 38 100 26 535.2 0 26 101 46 535.2 0 46 102 45 535.2 0 45 103 58 535.2 0 58 104 72 535.2 0 72 105 78 535.2 0 78 106 57 535.2 0 57 107 63 535.2 0 63 108 36 535.2 0 36 109 49 535.2 0 49 110 39 535.2 0 39 111 54 535.2 0 54 112 64 535.2 0 64 113 84 535.2 0 84 114 35 535.2 0 35 115 45 535.2 0 45 116 40 535.2 0 40 117 43 535.2 0 43 118 34 535.2 0 34 119 39 535.2 0 39 120 49 535.2 0 49 121 54 535.2 0 54 122 53 535.2 0 53 123 95 535.2 0 95 124 103 535.2 0 103 125 1067 535.2 0 1067 126 661 535.2 0 661 127 1258 535.2 0 1258 128 45 535.2 0 45 129 60 535.2 0 60 130 61 535.2 0 61 131 24 535.2 0 24 132 54 535.2 0 54 133 81 535.2 0 81 134 46 535.2 0 46 135 69 535.2 0 69 136 36 535.2 0 36 137 47 535.2 0 47 138 57 535.2 0 57 139 31 535.2 0 31 140 66 535.2 0 66 141 42 535.2 0 42 142 42 535.2 0 42 143 69 535.2 0 69 144 136 535.2 0 136 145 228 535.2 0 228 146 167 535.2 0 167 147 128 535.2 0 128 148 83 535.2 0 83 149 105 535.2 0 105 150 441 535.2 0 441 151 221 535.2 0 221 152 477 535.2 0 477 153 81 535.2 0 81 154 149 535.2 0 149 155 184 535.2 0 184 156 361 535.2 0 361 157 359 535.2 0 359 158 250 535.2 0 250 159 360 535.2 0 360 160 3695 535.2 0 3695 161 2757 535.2 0 2757 162 2115 535.2 0 2115 163 62 535.2 0 62 164 85 535.2 0 85 165 64 535.2 0 64 166 85 535.2 0 85 167 28 535.2 0 28 168 48 535.2 0 48 169 58 535.2 0 58 170 53 535.2 0 53 171 59 535.2 0 59 172 39 535.2 0 39 173 70 535.2 0 70 174 70 535.2 0 70 175 137 535.2 0 137 176 109 535.2 0 109 177 137 535.2 0 137 178 64 535.2 0 64 179 107 535.2 0 107 180 543 535.2 0 543 181 1054 535.2 0 1054 182 783 535.2 0 783 183 220 535.2 0 220 184 71 535.2 0 71 185 104 535.2 0 104 186 68 535.2 0 68 187 61 535.2 0 61 188 72 535.2 0 72 189 57 535.2 0 57 190 58 535.2 0 58 191 67 535.2 0 67 192 54 535.2 0 54 193 37 535.2 0 37 194 40 535.2 0 40 195 52 535.2 0 52 196 44 535.2 0 44 197 60 535.2 0 60 198 56 535.2 0 56 199 85 535.2 0 85 200 72 535.2 0 72 201 60 535.2 0 60 202 47 535.2 0 47 203 48 535.2 0 48 204 40 535.2 0 40 205 42 535.2 0 42 206 52 535.2 0 52 207 53 535.2 0 53 208 89 535.2 0 89 209 72 535.2 0 72 210 109 535.2 0 109 211 405 535.2 0 405 212 1782 535.2 0 1782 213 1407 535.2 0 1407 214 1462 535.2 0 1462 215 56 535.2 0 56 216 47 535.2 0 47 217 92 535.2 0 92 218 75 535.2 0 75 219 71 535.2 0 71 220 56 535.2 0 56 221 47 535.2 0 47 222 49 535.2 0 49 223 39 535.2 0 39 224 71 535.2 0 71 225 78 535.2 0 78 226 103 535.2 0 103 227 86 535.2 0 86 228 52 535.2 0 52 229 62 535.2 0 62 230 62 535.2 0 62 231 55 535.2 0 55 232 73 535.2 0 73 233 66 535.2 0 66 234 72 535.2 0 72 235 71 535.2 0 71 236 54 535.2 0 54 237 79 535.2 0 79 238 168 535.2 0 168 239 174 535.2 0 174 240 115 535.2 0 115 241 109 535.2 0 109 242 75 535.2 0 75 243 95 535.2 0 95 244 95 535.2 0 95 245 106 535.2 0 106 246 94 535.2 0 94 247 121 535.2 0 121 248 120 535.2 0 120 249 86 535.2 0 86 250 85 535.2 0 85 251 60 535.2 0 60 252 89 535.2 0 89 253 72 535.2 0 72 254 74 535.2 0 74 255 91 535.2 0 91 256 230 535.2 0 230 257 239 535.2 0 239 258 276 535.2 0 276 259 206 535.2 0 206 260 87 535.2 0 87 261 77 535.2 0 77 262 85 535.2 0 85 263 82 535.2 0 82 264 80 535.2 0 80 265 61 535.2 0 61 266 179 535.2 0 179 267 218 535.2 0 218 268 364 535.2 0 364 269 168 535.2 0 168 270 122 535.2 0 122 271 137 535.2 0 137 272 218 535.2 0 218 273 386 535.2 0 386 274 466 535.2 0 466 275 434 535.2 0 434 276 138 535.2 0 138 277 117 535.2 0 117 278 183 535.2 0 183 279 119 535.2 0 119 280 175 535.2 0 175 281 218 535.2 0 218 282 148 535.2 0 148 283 86 535.2 0 86 284 99 535.2 0 99 285 118 535.2 0 118 286 103 535.2 0 103 287 155 535.2 0 155 288 174 535.2 0 174 289 170 535.2 0 170 290 73 535.2 0 73 291 64 535.2 0 64 292 118 535.2 0 118 293 145 535.2 0 145 294 88 535.2 0 88 295 92 535.2 0 92 296 58 535.2 0 58 297 78 535.2 0 78 298 83 535.2 0 83 299 78 535.2 0 78 300 110 535.2 0 110 301 156 535.2 0 156 Finished in 3.61 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 83,277 Reads with adapters: 25,290 (30.4%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 25,252 (30.3%) Total basepairs processed: 20,934,404 bp Total written (filtered): 6,942,280 bp (33.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25290 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 132 0.0 3 0 131 1 17 24658 0.0 3 24207 433 16 2 18 412 0.0 3 357 52 2 1 19 13 0.0 3 1 0 10 2 20 14 0.0 3 0 0 1 13 33 3 0.0 3 0 0 0 3 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 1 1 62 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 1 101 1 0.0 3 0 1 107 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 218 3 0.0 3 0 0 0 3 238 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 25,252 Reads with adapters: 759 (3.0%) Reads written (passing filters): 25,252 (100.0%) Total basepairs processed: 6,942,280 bp Total written (filtered): 6,939,930 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 759 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.1% C: 4.1% G: 22.1% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 731 394.6 0 731 4 19 98.6 0 19 9 9 0.1 0 0 9