This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.78 s (23 us/read; 2.62 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 74,865 (2.4%) Reads that were too short: 29,738 (1.0%) Reads written (passing filters): 45,127 (1.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 8,120,755 bp (0.9%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 74865 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 58 755.5 0 58 7 62 755.5 0 62 8 54 755.5 0 54 9 336 755.5 0 336 10 123 755.5 0 123 11 6123 755.5 0 6123 12 17 755.5 0 17 13 5 755.5 0 5 14 4 755.5 0 4 15 7 755.5 0 7 16 1 755.5 0 1 17 6 755.5 0 6 18 4 755.5 0 4 19 3 755.5 0 3 20 6 755.5 0 6 21 8 755.5 0 8 22 5 755.5 0 5 23 4 755.5 0 4 24 2 755.5 0 2 25 3 755.5 0 3 26 4 755.5 0 4 27 3 755.5 0 3 28 2 755.5 0 2 29 18 755.5 0 18 30 2 755.5 0 2 31 5 755.5 0 5 32 5 755.5 0 5 33 5 755.5 0 5 34 9 755.5 0 9 35 5 755.5 0 5 36 7 755.5 0 7 37 7 755.5 0 7 38 1 755.5 0 1 39 9 755.5 0 9 40 4 755.5 0 4 41 7 755.5 0 7 42 8 755.5 0 8 43 9 755.5 0 9 44 7 755.5 0 7 45 15 755.5 0 15 46 5 755.5 0 5 47 6 755.5 0 6 48 12 755.5 0 12 49 7 755.5 0 7 50 944 755.5 0 944 51 10 755.5 0 10 52 11 755.5 0 11 53 5 755.5 0 5 54 11 755.5 0 11 55 10 755.5 0 10 56 7 755.5 0 7 57 13 755.5 0 13 58 7 755.5 0 7 59 2 755.5 0 2 60 6 755.5 0 6 61 8 755.5 0 8 62 5 755.5 0 5 63 7 755.5 0 7 64 5 755.5 0 5 65 12 755.5 0 12 66 8 755.5 0 8 67 5 755.5 0 5 68 10 755.5 0 10 69 54 755.5 0 54 70 106 755.5 0 106 71 1757 755.5 0 1757 72 57 755.5 0 57 73 95 755.5 0 95 74 63 755.5 0 63 75 20 755.5 0 20 76 3 755.5 0 3 77 5 755.5 0 5 78 3 755.5 0 3 79 19 755.5 0 19 80 80 755.5 0 80 81 74 755.5 0 74 82 90 755.5 0 90 83 74 755.5 0 74 84 147 755.5 0 147 85 41 755.5 0 41 86 40 755.5 0 40 87 39 755.5 0 39 88 33 755.5 0 33 89 15 755.5 0 15 90 102 755.5 0 102 91 55 755.5 0 55 92 384 755.5 0 384 93 64 755.5 0 64 94 39 755.5 0 39 95 20 755.5 0 20 96 53 755.5 0 53 97 109 755.5 0 109 98 145 755.5 0 145 99 116 755.5 0 116 100 235 755.5 0 235 101 666 755.5 0 666 102 559 755.5 0 559 103 964 755.5 0 964 104 418 755.5 0 418 105 63 755.5 0 63 106 110 755.5 0 110 107 57 755.5 0 57 108 67 755.5 0 67 109 121 755.5 0 121 110 140 755.5 0 140 111 164 755.5 0 164 112 135 755.5 0 135 113 201 755.5 0 201 114 291 755.5 0 291 115 183 755.5 0 183 116 339 755.5 0 339 117 194 755.5 0 194 118 207 755.5 0 207 119 126 755.5 0 126 120 99 755.5 0 99 121 144 755.5 0 144 122 149 755.5 0 149 123 481 755.5 0 481 124 434 755.5 0 434 125 359 755.5 0 359 126 132 755.5 0 132 127 59 755.5 0 59 128 53 755.5 0 53 129 91 755.5 0 91 130 609 755.5 0 609 131 513 755.5 0 513 132 677 755.5 0 677 133 438 755.5 0 438 134 216 755.5 0 216 135 160 755.5 0 160 136 231 755.5 0 231 137 413 755.5 0 413 138 437 755.5 0 437 139 308 755.5 0 308 140 67 755.5 0 67 141 176 755.5 0 176 142 511 755.5 0 511 143 531 755.5 0 531 144 987 755.5 0 987 145 375 755.5 0 375 146 227 755.5 0 227 147 118 755.5 0 118 148 204 755.5 0 204 149 801 755.5 0 801 150 269 755.5 0 269 151 489 755.5 0 489 152 216 755.5 0 216 153 184 755.5 0 184 154 458 755.5 0 458 155 381 755.5 0 381 156 537 755.5 0 537 157 930 755.5 0 930 158 824 755.5 0 824 159 301 755.5 0 301 160 229 755.5 0 229 161 141 755.5 0 141 162 243 755.5 0 243 163 200 755.5 0 200 164 357 755.5 0 357 165 193 755.5 0 193 166 235 755.5 0 235 167 219 755.5 0 219 168 259 755.5 0 259 169 727 755.5 0 727 170 1027 755.5 0 1027 171 1349 755.5 0 1349 172 308 755.5 0 308 173 141 755.5 0 141 174 44 755.5 0 44 175 90 755.5 0 90 176 294 755.5 0 294 177 972 755.5 0 972 178 706 755.5 0 706 179 548 755.5 0 548 180 279 755.5 0 279 181 109 755.5 0 109 182 192 755.5 0 192 183 174 755.5 0 174 184 262 755.5 0 262 185 135 755.5 0 135 186 102 755.5 0 102 187 233 755.5 0 233 188 625 755.5 0 625 189 578 755.5 0 578 190 148 755.5 0 148 191 115 755.5 0 115 192 71 755.5 0 71 193 61 755.5 0 61 194 74 755.5 0 74 195 323 755.5 0 323 196 193 755.5 0 193 197 360 755.5 0 360 198 173 755.5 0 173 199 232 755.5 0 232 200 291 755.5 0 291 201 305 755.5 0 305 202 168 755.5 0 168 203 136 755.5 0 136 204 172 755.5 0 172 205 215 755.5 0 215 206 206 755.5 0 206 207 192 755.5 0 192 208 130 755.5 0 130 209 445 755.5 0 445 210 651 755.5 0 651 211 559 755.5 0 559 212 403 755.5 0 403 213 213 755.5 0 213 214 268 755.5 0 268 215 312 755.5 0 312 216 348 755.5 0 348 217 283 755.5 0 283 218 236 755.5 0 236 219 123 755.5 0 123 220 100 755.5 0 100 221 273 755.5 0 273 222 270 755.5 0 270 223 513 755.5 0 513 224 304 755.5 0 304 225 411 755.5 0 411 226 399 755.5 0 399 227 285 755.5 0 285 228 199 755.5 0 199 229 193 755.5 0 193 230 113 755.5 0 113 231 118 755.5 0 118 232 203 755.5 0 203 233 175 755.5 0 175 234 159 755.5 0 159 235 99 755.5 0 99 236 96 755.5 0 96 237 156 755.5 0 156 238 169 755.5 0 169 239 183 755.5 0 183 240 150 755.5 0 150 241 220 755.5 0 220 242 278 755.5 0 278 243 330 755.5 0 330 244 363 755.5 0 363 245 442 755.5 0 442 246 260 755.5 0 260 247 270 755.5 0 270 248 304 755.5 0 304 249 340 755.5 0 340 250 395 755.5 0 395 251 215 755.5 0 215 252 367 755.5 0 367 253 268 755.5 0 268 254 238 755.5 0 238 255 189 755.5 0 189 256 157 755.5 0 157 257 167 755.5 0 167 258 203 755.5 0 203 259 272 755.5 0 272 260 256 755.5 0 256 261 270 755.5 0 270 262 224 755.5 0 224 263 419 755.5 0 419 264 1091 755.5 0 1091 265 624 755.5 0 624 266 293 755.5 0 293 267 80 755.5 0 80 268 135 755.5 0 135 269 132 755.5 0 132 270 419 755.5 0 419 271 457 755.5 0 457 272 704 755.5 0 704 273 417 755.5 0 417 274 308 755.5 0 308 275 229 755.5 0 229 276 347 755.5 0 347 277 419 755.5 0 419 278 266 755.5 0 266 279 114 755.5 0 114 280 159 755.5 0 159 281 354 755.5 0 354 282 401 755.5 0 401 283 894 755.5 0 894 284 1000 755.5 0 1000 285 807 755.5 0 807 286 382 755.5 0 382 287 129 755.5 0 129 288 97 755.5 0 97 289 143 755.5 0 143 290 253 755.5 0 253 291 277 755.5 0 277 292 172 755.5 0 172 293 170 755.5 0 170 294 83 755.5 0 83 295 85 755.5 0 85 296 188 755.5 0 188 297 254 755.5 0 254 298 389 755.5 0 389 299 611 755.5 0 611 300 544 755.5 0 544 301 236 755.5 0 236 Finished in 1.34 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 45,127 Reads with adapters: 331 (0.7%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 319 (0.7%) Total basepairs processed: 8,120,755 bp Total written (filtered): 83,718 bp (1.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 331 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 2 17 284 0.0 3 280 4 18 3 0.0 3 0 1 2 19 2 0.0 3 1 0 1 20 1 0.0 3 1 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 91 2 0.0 3 0 0 0 2 113 3 0.0 3 0 1 0 2 119 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 181 4 0.0 3 0 0 0 4 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 274 3 0.0 3 0 0 0 3 275 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 319 Reads with adapters: 7 (2.2%) Reads written (passing filters): 319 (100.0%) Total basepairs processed: 83,718 bp Total written (filtered): 83,697 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 7 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 71.4% C: 14.3% G: 14.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 7 5.0 0 7