This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.80 s (23 us/read; 2.66 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 42,896 (1.4%) Reads that were too short: 11,297 (0.4%) Reads written (passing filters): 31,599 (1.0%) Total basepairs processed: 931,490,553 bp Total written (filtered): 8,131,699 bp (0.9%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 42896 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 228 755.5 0 228 7 398 755.5 0 398 8 21552 755.5 0 21552 9 197 755.5 0 197 10 11 755.5 0 11 11 8 755.5 0 8 12 13 755.5 0 13 13 15 755.5 0 15 14 10 755.5 0 10 15 10 755.5 0 10 16 10 755.5 0 10 17 12 755.5 0 12 18 20 755.5 0 20 19 15 755.5 0 15 20 8 755.5 0 8 21 14 755.5 0 14 22 11 755.5 0 11 23 10 755.5 0 10 24 11 755.5 0 11 25 12 755.5 0 12 26 9 755.5 0 9 27 13 755.5 0 13 28 9 755.5 0 9 29 7 755.5 0 7 30 10 755.5 0 10 31 10 755.5 0 10 32 18 755.5 0 18 33 11 755.5 0 11 34 13 755.5 0 13 35 4 755.5 0 4 36 7 755.5 0 7 37 20 755.5 0 20 38 44 755.5 0 44 39 11 755.5 0 11 40 10 755.5 0 10 41 14 755.5 0 14 42 18 755.5 0 18 43 8 755.5 0 8 44 14 755.5 0 14 45 8 755.5 0 8 46 7 755.5 0 7 47 7 755.5 0 7 48 6 755.5 0 6 49 20 755.5 0 20 50 9 755.5 0 9 51 47 755.5 0 47 52 23 755.5 0 23 53 13 755.5 0 13 54 60 755.5 0 60 55 11 755.5 0 11 56 11 755.5 0 11 57 46 755.5 0 46 58 11 755.5 0 11 59 9 755.5 0 9 60 11 755.5 0 11 61 9 755.5 0 9 62 11 755.5 0 11 63 10 755.5 0 10 64 6 755.5 0 6 65 8 755.5 0 8 66 31 755.5 0 31 67 51 755.5 0 51 68 15 755.5 0 15 69 8 755.5 0 8 70 14 755.5 0 14 71 11 755.5 0 11 72 12 755.5 0 12 73 8 755.5 0 8 74 34 755.5 0 34 75 28 755.5 0 28 76 66 755.5 0 66 77 8 755.5 0 8 78 30 755.5 0 30 79 44 755.5 0 44 80 56 755.5 0 56 81 16 755.5 0 16 82 35 755.5 0 35 83 28 755.5 0 28 84 23 755.5 0 23 85 56 755.5 0 56 86 27 755.5 0 27 87 24 755.5 0 24 88 20 755.5 0 20 89 38 755.5 0 38 90 21 755.5 0 21 91 58 755.5 0 58 92 32 755.5 0 32 93 37 755.5 0 37 94 88 755.5 0 88 95 315 755.5 0 315 96 70 755.5 0 70 97 299 755.5 0 299 98 233 755.5 0 233 99 272 755.5 0 272 100 285 755.5 0 285 101 18 755.5 0 18 102 36 755.5 0 36 103 17 755.5 0 17 104 11 755.5 0 11 105 14 755.5 0 14 106 22 755.5 0 22 107 22 755.5 0 22 108 33 755.5 0 33 109 50 755.5 0 50 110 18 755.5 0 18 111 67 755.5 0 67 112 70 755.5 0 70 113 428 755.5 0 428 114 30 755.5 0 30 115 35 755.5 0 35 116 30 755.5 0 30 117 48 755.5 0 48 118 22 755.5 0 22 119 20 755.5 0 20 120 41 755.5 0 41 121 34 755.5 0 34 122 19 755.5 0 19 123 29 755.5 0 29 124 16 755.5 0 16 125 32 755.5 0 32 126 41 755.5 0 41 127 32 755.5 0 32 128 41 755.5 0 41 129 99 755.5 0 99 130 397 755.5 0 397 131 38 755.5 0 38 132 69 755.5 0 69 133 56 755.5 0 56 134 137 755.5 0 137 135 68 755.5 0 68 136 69 755.5 0 69 137 36 755.5 0 36 138 37 755.5 0 37 139 28 755.5 0 28 140 60 755.5 0 60 141 27 755.5 0 27 142 33 755.5 0 33 143 21 755.5 0 21 144 26 755.5 0 26 145 32 755.5 0 32 146 69 755.5 0 69 147 48 755.5 0 48 148 40 755.5 0 40 149 31 755.5 0 31 150 24 755.5 0 24 151 30 755.5 0 30 152 18 755.5 0 18 153 15 755.5 0 15 154 29 755.5 0 29 155 45 755.5 0 45 156 116 755.5 0 116 157 35 755.5 0 35 158 28 755.5 0 28 159 30 755.5 0 30 160 25 755.5 0 25 161 27 755.5 0 27 162 35 755.5 0 35 163 24 755.5 0 24 164 36 755.5 0 36 165 37 755.5 0 37 166 96 755.5 0 96 167 67 755.5 0 67 168 53 755.5 0 53 169 39 755.5 0 39 170 25 755.5 0 25 171 22 755.5 0 22 172 22 755.5 0 22 173 60 755.5 0 60 174 54 755.5 0 54 175 67 755.5 0 67 176 55 755.5 0 55 177 15 755.5 0 15 178 29 755.5 0 29 179 30 755.5 0 30 180 35 755.5 0 35 181 98 755.5 0 98 182 65 755.5 0 65 183 47 755.5 0 47 184 46 755.5 0 46 185 72 755.5 0 72 186 110 755.5 0 110 187 53 755.5 0 53 188 21 755.5 0 21 189 20 755.5 0 20 190 18 755.5 0 18 191 31 755.5 0 31 192 67 755.5 0 67 193 500 755.5 0 500 194 269 755.5 0 269 195 105 755.5 0 105 196 148 755.5 0 148 197 57 755.5 0 57 198 43 755.5 0 43 199 101 755.5 0 101 200 104 755.5 0 104 201 153 755.5 0 153 202 184 755.5 0 184 203 40 755.5 0 40 204 32 755.5 0 32 205 71 755.5 0 71 206 55 755.5 0 55 207 75 755.5 0 75 208 45 755.5 0 45 209 34 755.5 0 34 210 48 755.5 0 48 211 74 755.5 0 74 212 85 755.5 0 85 213 52 755.5 0 52 214 44 755.5 0 44 215 55 755.5 0 55 216 68 755.5 0 68 217 86 755.5 0 86 218 82 755.5 0 82 219 78 755.5 0 78 220 131 755.5 0 131 221 183 755.5 0 183 222 526 755.5 0 526 223 107 755.5 0 107 224 19 755.5 0 19 225 60 755.5 0 60 226 49 755.5 0 49 227 89 755.5 0 89 228 75 755.5 0 75 229 59 755.5 0 59 230 31 755.5 0 31 231 28 755.5 0 28 232 26 755.5 0 26 233 57 755.5 0 57 234 39 755.5 0 39 235 40 755.5 0 40 236 65 755.5 0 65 237 77 755.5 0 77 238 101 755.5 0 101 239 67 755.5 0 67 240 25 755.5 0 25 241 38 755.5 0 38 242 48 755.5 0 48 243 167 755.5 0 167 244 89 755.5 0 89 245 91 755.5 0 91 246 103 755.5 0 103 247 77 755.5 0 77 248 85 755.5 0 85 249 46 755.5 0 46 250 53 755.5 0 53 251 72 755.5 0 72 252 76 755.5 0 76 253 110 755.5 0 110 254 209 755.5 0 209 255 457 755.5 0 457 256 3559 755.5 0 3559 257 108 755.5 0 108 258 80 755.5 0 80 259 83 755.5 0 83 260 27 755.5 0 27 261 27 755.5 0 27 262 107 755.5 0 107 263 507 755.5 0 507 264 388 755.5 0 388 265 83 755.5 0 83 266 107 755.5 0 107 267 71 755.5 0 71 268 51 755.5 0 51 269 67 755.5 0 67 270 31 755.5 0 31 271 33 755.5 0 33 272 38 755.5 0 38 273 50 755.5 0 50 274 30 755.5 0 30 275 73 755.5 0 73 276 65 755.5 0 65 277 19 755.5 0 19 278 14 755.5 0 14 279 43 755.5 0 43 280 73 755.5 0 73 281 62 755.5 0 62 282 37 755.5 0 37 283 23 755.5 0 23 284 21 755.5 0 21 285 15 755.5 0 15 286 42 755.5 0 42 287 73 755.5 0 73 288 27 755.5 0 27 289 19 755.5 0 19 290 15 755.5 0 15 291 46 755.5 0 46 292 75 755.5 0 75 293 47 755.5 0 47 294 39 755.5 0 39 295 30 755.5 0 30 296 30 755.5 0 30 297 21 755.5 0 21 298 32 755.5 0 32 299 59 755.5 0 59 300 42 755.5 0 42 301 25 755.5 0 25 Finished in 1.37 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 31,599 Reads with adapters: 205 (0.6%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 192 (0.6%) Total basepairs processed: 8,131,699 bp Total written (filtered): 48,153 bp (0.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 205 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 4 0.0 3 0 4 20 146 0.0 4 142 3 1 22 1 0.0 4 1 23 4 0.0 4 3 0 0 1 36 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 30 0.0 4 0 0 0 0 30 134 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 188 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 3 0.0 4 0 0 0 0 3 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 192 Reads with adapters: 32 (16.7%) Reads written (passing filters): 192 (100.0%) Total basepairs processed: 48,153 bp Total written (filtered): 43,380 bp (90.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 32 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.4% C: 53.1% G: 37.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 1 0.0 0 0 1 10 3 0.0 1 0 3 11 7 0.0 1 0 7 12 1 0.0 1 0 1 223 15 0.0 1 15 257 1 0.0 1 1 258 1 0.0 1 1 259 1 0.0 1 1 263 2 0.0 1 2