This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.01 s (23 us/read; 2.58 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 158,978 (5.1%) Reads that were too short: 29,300 (0.9%) Reads written (passing filters): 129,678 (4.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 26,913,837 bp (2.9%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 158978 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 16 755.5 0 16 7 16 755.5 0 16 8 172 755.5 0 172 9 11 755.5 0 11 10 3 755.5 0 3 11 32 755.5 0 32 12 19 755.5 0 19 13 3 755.5 0 3 14 1 755.5 0 1 15 4 755.5 0 4 16 4 755.5 0 4 17 2 755.5 0 2 18 6 755.5 0 6 19 3 755.5 0 3 20 8 755.5 0 8 21 427 755.5 0 427 22 39 755.5 0 39 23 17 755.5 0 17 24 9 755.5 0 9 25 12 755.5 0 12 26 4 755.5 0 4 27 5 755.5 0 5 28 6 755.5 0 6 29 5 755.5 0 5 30 2 755.5 0 2 31 3 755.5 0 3 32 5 755.5 0 5 33 17 755.5 0 17 34 6 755.5 0 6 35 1 755.5 0 1 36 3 755.5 0 3 37 3 755.5 0 3 38 20 755.5 0 20 39 34 755.5 0 34 40 7 755.5 0 7 41 6 755.5 0 6 42 7 755.5 0 7 43 8 755.5 0 8 44 4 755.5 0 4 45 12 755.5 0 12 46 12 755.5 0 12 47 6 755.5 0 6 48 8 755.5 0 8 49 11 755.5 0 11 50 11 755.5 0 11 51 10 755.5 0 10 52 7 755.5 0 7 53 9 755.5 0 9 54 20 755.5 0 20 55 22 755.5 0 22 56 2313 755.5 0 2313 57 228 755.5 0 228 58 82 755.5 0 82 59 49 755.5 0 49 60 31 755.5 0 31 61 186 755.5 0 186 62 19 755.5 0 19 63 46 755.5 0 46 64 18 755.5 0 18 65 38 755.5 0 38 66 21 755.5 0 21 67 19 755.5 0 19 68 10 755.5 0 10 69 43 755.5 0 43 70 17 755.5 0 17 71 45 755.5 0 45 72 38 755.5 0 38 73 63 755.5 0 63 74 107 755.5 0 107 75 191 755.5 0 191 76 440 755.5 0 440 77 74 755.5 0 74 78 120 755.5 0 120 79 337 755.5 0 337 80 239 755.5 0 239 81 213 755.5 0 213 82 361 755.5 0 361 83 773 755.5 0 773 84 382 755.5 0 382 85 689 755.5 0 689 86 639 755.5 0 639 87 675 755.5 0 675 88 810 755.5 0 810 89 1709 755.5 0 1709 90 9406 755.5 0 9406 91 99080 755.5 0 99080 92 425 755.5 0 425 93 9 755.5 0 9 94 15 755.5 0 15 95 26 755.5 0 26 96 44 755.5 0 44 97 20 755.5 0 20 98 13 755.5 0 13 99 26 755.5 0 26 100 29 755.5 0 29 101 43 755.5 0 43 102 195 755.5 0 195 103 10 755.5 0 10 104 10 755.5 0 10 105 26 755.5 0 26 106 15 755.5 0 15 107 9 755.5 0 9 108 18 755.5 0 18 109 4 755.5 0 4 110 20 755.5 0 20 111 31 755.5 0 31 112 178 755.5 0 178 113 6 755.5 0 6 114 25 755.5 0 25 115 13 755.5 0 13 116 29 755.5 0 29 117 8 755.5 0 8 118 4 755.5 0 4 119 7 755.5 0 7 120 72 755.5 0 72 121 32 755.5 0 32 122 23 755.5 0 23 123 9 755.5 0 9 124 37 755.5 0 37 125 20 755.5 0 20 126 26 755.5 0 26 127 27 755.5 0 27 128 9 755.5 0 9 129 11 755.5 0 11 130 7 755.5 0 7 131 12 755.5 0 12 132 347 755.5 0 347 133 40 755.5 0 40 134 18 755.5 0 18 135 24 755.5 0 24 136 11 755.5 0 11 137 13 755.5 0 13 138 16 755.5 0 16 139 28 755.5 0 28 140 72 755.5 0 72 141 29 755.5 0 29 142 16 755.5 0 16 143 8 755.5 0 8 144 10 755.5 0 10 145 22 755.5 0 22 146 25 755.5 0 25 147 43 755.5 0 43 148 196 755.5 0 196 149 633 755.5 0 633 150 1558 755.5 0 1558 151 7 755.5 0 7 152 9 755.5 0 9 153 13 755.5 0 13 154 8 755.5 0 8 155 15 755.5 0 15 156 15 755.5 0 15 157 10 755.5 0 10 158 17 755.5 0 17 159 30 755.5 0 30 160 39 755.5 0 39 161 21 755.5 0 21 162 16 755.5 0 16 163 11 755.5 0 11 164 12 755.5 0 12 165 36 755.5 0 36 166 63 755.5 0 63 167 77 755.5 0 77 168 793 755.5 0 793 169 946 755.5 0 946 170 119 755.5 0 119 171 10 755.5 0 10 172 16 755.5 0 16 173 24 755.5 0 24 174 39 755.5 0 39 175 77 755.5 0 77 176 755 755.5 0 755 177 165 755.5 0 165 178 582 755.5 0 582 179 30 755.5 0 30 180 21 755.5 0 21 181 23 755.5 0 23 182 16 755.5 0 16 183 21 755.5 0 21 184 41 755.5 0 41 185 51 755.5 0 51 186 53 755.5 0 53 187 10 755.5 0 10 188 4 755.5 0 4 189 5 755.5 0 5 190 8 755.5 0 8 191 18 755.5 0 18 192 15 755.5 0 15 193 46 755.5 0 46 194 36 755.5 0 36 195 14 755.5 0 14 196 16 755.5 0 16 197 10 755.5 0 10 198 21 755.5 0 21 199 21 755.5 0 21 200 14 755.5 0 14 201 18 755.5 0 18 202 20 755.5 0 20 203 32 755.5 0 32 204 25 755.5 0 25 205 57 755.5 0 57 206 69 755.5 0 69 207 239 755.5 0 239 208 130 755.5 0 130 209 100 755.5 0 100 210 47 755.5 0 47 211 30 755.5 0 30 212 37 755.5 0 37 213 74 755.5 0 74 214 59 755.5 0 59 215 106 755.5 0 106 216 137 755.5 0 137 217 69 755.5 0 69 218 105 755.5 0 105 219 276 755.5 0 276 220 1815 755.5 0 1815 221 1011 755.5 0 1011 222 2216 755.5 0 2216 223 30 755.5 0 30 224 27 755.5 0 27 225 65 755.5 0 65 226 59 755.5 0 59 227 44 755.5 0 44 228 59 755.5 0 59 229 51 755.5 0 51 230 67 755.5 0 67 231 47 755.5 0 47 232 42 755.5 0 42 233 23 755.5 0 23 234 18 755.5 0 18 235 38 755.5 0 38 236 38 755.5 0 38 237 72 755.5 0 72 238 48 755.5 0 48 239 69 755.5 0 69 240 64 755.5 0 64 241 100 755.5 0 100 242 34 755.5 0 34 243 24 755.5 0 24 244 27 755.5 0 27 245 57 755.5 0 57 246 355 755.5 0 355 247 49 755.5 0 49 248 27 755.5 0 27 249 7 755.5 0 7 250 6 755.5 0 6 251 20 755.5 0 20 252 15 755.5 0 15 253 24 755.5 0 24 254 57 755.5 0 57 255 162 755.5 0 162 256 140 755.5 0 140 257 55 755.5 0 55 258 64 755.5 0 64 259 158 755.5 0 158 260 22 755.5 0 22 261 30 755.5 0 30 262 30 755.5 0 30 263 42 755.5 0 42 264 61 755.5 0 61 265 123 755.5 0 123 266 117 755.5 0 117 267 88 755.5 0 88 268 83 755.5 0 83 269 125 755.5 0 125 270 277 755.5 0 277 271 333 755.5 0 333 272 80 755.5 0 80 273 53 755.5 0 53 274 578 755.5 0 578 275 193 755.5 0 193 276 1309 755.5 0 1309 277 38 755.5 0 38 278 54 755.5 0 54 279 100 755.5 0 100 280 109 755.5 0 109 281 374 755.5 0 374 282 699 755.5 0 699 283 8763 755.5 0 8763 284 2306 755.5 0 2306 285 3194 755.5 0 3194 286 454 755.5 0 454 287 53 755.5 0 53 288 33 755.5 0 33 289 37 755.5 0 37 290 40 755.5 0 40 291 20 755.5 0 20 292 24 755.5 0 24 293 90 755.5 0 90 294 208 755.5 0 208 295 70 755.5 0 70 296 94 755.5 0 94 297 21 755.5 0 21 298 8 755.5 0 8 299 17 755.5 0 17 300 14 755.5 0 14 301 40 755.5 0 40 Finished in 4.14 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 129,678 Reads with adapters: 406 (0.3%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 399 (0.3%) Total basepairs processed: 26,913,837 bp Total written (filtered): 74,964 bp (0.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 406 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 2 17 167 0.0 3 162 2 0 3 18 10 0.0 3 0 2 7 1 19 4 0.0 3 1 0 0 3 20 5 0.0 3 2 1 1 1 23 1 0.0 3 0 1 43 15 0.0 3 0 0 0 15 46 9 0.0 3 0 0 0 9 56 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 1 98 1 0.0 3 0 0 0 1 101 12 0.0 3 0 0 0 12 102 126 0.0 3 0 0 0 126 103 16 0.0 3 0 0 0 16 105 26 0.0 3 0 0 0 26 135 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 203 6 0.0 3 0 0 0 6 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 399 Reads with adapters: 9 (2.3%) Reads written (passing filters): 399 (100.0%) Total basepairs processed: 74,964 bp Total written (filtered): 74,937 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 9 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.2% C: 22.2% G: 11.1% T: 44.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 6.2 0 9