This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 79.98 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 73,512 (2.4%) Reads that were too short: 6,471 (0.2%) Reads written (passing filters): 67,041 (2.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 15,452,538 bp (1.7%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 73512 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 323 755.5 0 323 7 2898 755.5 0 2898 8 10223 755.5 0 10223 9 46 755.5 0 46 10 5 755.5 0 5 11 124 755.5 0 124 12 34 755.5 0 34 13 24 755.5 0 24 14 75 755.5 0 75 15 11 755.5 0 11 16 9 755.5 0 9 17 14 755.5 0 14 18 22 755.5 0 22 19 19 755.5 0 19 20 23 755.5 0 23 21 19 755.5 0 19 22 14 755.5 0 14 23 21 755.5 0 21 24 16 755.5 0 16 25 12 755.5 0 12 26 26 755.5 0 26 27 23 755.5 0 23 28 16 755.5 0 16 29 63 755.5 0 63 30 19 755.5 0 19 31 15 755.5 0 15 32 17 755.5 0 17 33 16 755.5 0 16 34 12 755.5 0 12 35 12 755.5 0 12 36 27 755.5 0 27 37 20 755.5 0 20 38 43 755.5 0 43 39 140 755.5 0 140 40 32 755.5 0 32 41 50 755.5 0 50 42 30 755.5 0 30 43 39 755.5 0 39 44 82 755.5 0 82 45 57 755.5 0 57 46 58 755.5 0 58 47 29 755.5 0 29 48 49 755.5 0 49 49 107 755.5 0 107 50 123 755.5 0 123 51 202 755.5 0 202 52 216 755.5 0 216 53 247 755.5 0 247 54 548 755.5 0 548 55 944 755.5 0 944 56 19026 755.5 0 19026 57 4978 755.5 0 4978 58 9789 755.5 0 9789 59 60 755.5 0 60 60 18 755.5 0 18 61 44 755.5 0 44 62 747 755.5 0 747 63 38 755.5 0 38 64 23 755.5 0 23 65 7 755.5 0 7 66 14 755.5 0 14 67 26 755.5 0 26 68 11 755.5 0 11 69 9 755.5 0 9 70 23 755.5 0 23 71 14 755.5 0 14 72 56 755.5 0 56 73 21 755.5 0 21 74 13 755.5 0 13 75 18 755.5 0 18 76 11 755.5 0 11 77 12 755.5 0 12 78 21 755.5 0 21 79 44 755.5 0 44 80 24 755.5 0 24 81 25 755.5 0 25 82 30 755.5 0 30 83 127 755.5 0 127 84 68 755.5 0 68 85 101 755.5 0 101 86 43 755.5 0 43 87 67 755.5 0 67 88 79 755.5 0 79 89 286 755.5 0 286 90 100 755.5 0 100 91 20 755.5 0 20 92 12 755.5 0 12 93 47 755.5 0 47 94 60 755.5 0 60 95 23 755.5 0 23 96 19 755.5 0 19 97 20 755.5 0 20 98 8 755.5 0 8 99 16 755.5 0 16 100 16 755.5 0 16 101 25 755.5 0 25 102 18 755.5 0 18 103 33 755.5 0 33 104 42 755.5 0 42 105 148 755.5 0 148 106 12 755.5 0 12 107 16 755.5 0 16 108 9 755.5 0 9 109 13 755.5 0 13 110 15 755.5 0 15 111 29 755.5 0 29 112 22 755.5 0 22 113 24 755.5 0 24 114 23 755.5 0 23 115 15 755.5 0 15 116 23 755.5 0 23 117 35 755.5 0 35 118 17 755.5 0 17 119 194 755.5 0 194 120 56 755.5 0 56 121 55 755.5 0 55 122 31 755.5 0 31 123 27 755.5 0 27 124 26 755.5 0 26 125 46 755.5 0 46 126 130 755.5 0 130 127 26 755.5 0 26 128 101 755.5 0 101 129 15 755.5 0 15 130 28 755.5 0 28 131 26 755.5 0 26 132 32 755.5 0 32 133 36 755.5 0 36 134 26 755.5 0 26 135 34 755.5 0 34 136 29 755.5 0 29 137 24 755.5 0 24 138 43 755.5 0 43 139 46 755.5 0 46 140 46 755.5 0 46 141 104 755.5 0 104 142 179 755.5 0 179 143 30 755.5 0 30 144 30 755.5 0 30 145 70 755.5 0 70 146 96 755.5 0 96 147 680 755.5 0 680 148 246 755.5 0 246 149 87 755.5 0 87 150 173 755.5 0 173 151 51 755.5 0 51 152 183 755.5 0 183 153 44 755.5 0 44 154 41 755.5 0 41 155 27 755.5 0 27 156 41 755.5 0 41 157 54 755.5 0 54 158 47 755.5 0 47 159 48 755.5 0 48 160 112 755.5 0 112 161 41 755.5 0 41 162 65 755.5 0 65 163 59 755.5 0 59 164 50 755.5 0 50 165 82 755.5 0 82 166 138 755.5 0 138 167 1015 755.5 0 1015 168 470 755.5 0 470 169 364 755.5 0 364 170 77 755.5 0 77 171 146 755.5 0 146 172 222 755.5 0 222 173 236 755.5 0 236 174 121 755.5 0 121 175 105 755.5 0 105 176 83 755.5 0 83 177 231 755.5 0 231 178 227 755.5 0 227 179 51 755.5 0 51 180 52 755.5 0 52 181 83 755.5 0 83 182 68 755.5 0 68 183 44 755.5 0 44 184 48 755.5 0 48 185 48 755.5 0 48 186 66 755.5 0 66 187 22 755.5 0 22 188 37 755.5 0 37 189 27 755.5 0 27 190 55 755.5 0 55 191 72 755.5 0 72 192 110 755.5 0 110 193 88 755.5 0 88 194 109 755.5 0 109 195 138 755.5 0 138 196 257 755.5 0 257 197 491 755.5 0 491 198 121 755.5 0 121 199 307 755.5 0 307 200 3230 755.5 0 3230 201 438 755.5 0 438 202 849 755.5 0 849 203 42 755.5 0 42 204 36 755.5 0 36 205 42 755.5 0 42 206 26 755.5 0 26 207 49 755.5 0 49 208 18 755.5 0 18 209 15 755.5 0 15 210 11 755.5 0 11 211 22 755.5 0 22 212 21 755.5 0 21 213 36 755.5 0 36 214 28 755.5 0 28 215 34 755.5 0 34 216 46 755.5 0 46 217 50 755.5 0 50 218 38 755.5 0 38 219 90 755.5 0 90 220 31 755.5 0 31 221 72 755.5 0 72 222 29 755.5 0 29 223 62 755.5 0 62 224 85 755.5 0 85 225 52 755.5 0 52 226 36 755.5 0 36 227 48 755.5 0 48 228 53 755.5 0 53 229 121 755.5 0 121 230 46 755.5 0 46 231 80 755.5 0 80 232 280 755.5 0 280 233 59 755.5 0 59 234 71 755.5 0 71 235 22 755.5 0 22 236 13 755.5 0 13 237 13 755.5 0 13 238 12 755.5 0 12 239 12 755.5 0 12 240 18 755.5 0 18 241 31 755.5 0 31 242 47 755.5 0 47 243 35 755.5 0 35 244 56 755.5 0 56 245 50 755.5 0 50 246 20 755.5 0 20 247 9 755.5 0 9 248 25 755.5 0 25 249 42 755.5 0 42 250 26 755.5 0 26 251 49 755.5 0 49 252 136 755.5 0 136 253 126 755.5 0 126 254 85 755.5 0 85 255 76 755.5 0 76 256 32 755.5 0 32 257 18 755.5 0 18 258 13 755.5 0 13 259 13 755.5 0 13 260 10 755.5 0 10 261 45 755.5 0 45 262 26 755.5 0 26 263 50 755.5 0 50 264 64 755.5 0 64 265 101 755.5 0 101 266 81 755.5 0 81 267 18 755.5 0 18 268 22 755.5 0 22 269 30 755.5 0 30 270 30 755.5 0 30 271 36 755.5 0 36 272 42 755.5 0 42 273 74 755.5 0 74 274 23 755.5 0 23 275 33 755.5 0 33 276 37 755.5 0 37 277 51 755.5 0 51 278 86 755.5 0 86 279 157 755.5 0 157 280 285 755.5 0 285 281 25 755.5 0 25 282 37 755.5 0 37 283 81 755.5 0 81 284 35 755.5 0 35 285 14 755.5 0 14 286 23 755.5 0 23 287 22 755.5 0 22 288 20 755.5 0 20 289 54 755.5 0 54 290 42 755.5 0 42 291 120 755.5 0 120 292 409 755.5 0 409 293 352 755.5 0 352 294 90 755.5 0 90 295 46 755.5 0 46 296 18 755.5 0 18 297 19 755.5 0 19 298 46 755.5 0 46 299 113 755.5 0 113 300 38 755.5 0 38 301 9 755.5 0 9 Finished in 2.88 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 67,041 Reads with adapters: 10,788 (16.1%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 10,775 (16.1%) Total basepairs processed: 15,452,538 bp Total written (filtered): 2,939,112 bp (19.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10788 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 1 0.0 3 0 0 1 19 116 0.0 3 0 92 8 12 4 20 10422 0.0 4 10110 283 22 5 2 21 28 0.0 4 1 24 1 0 2 22 10 0.0 4 2 2 6 23 173 0.0 4 153 8 0 12 47 1 0.0 4 0 0 0 0 1 56 3 0.0 4 0 0 0 0 3 57 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 21 0.0 4 0 0 0 0 21 134 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 10,775 Reads with adapters: 1,630 (15.1%) Reads written (passing filters): 10,775 (100.0%) Total basepairs processed: 2,939,112 bp Total written (filtered): 2,891,839 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1630 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 1.7% G: 88.3% T: 8.4% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 168.4 0 1 9 2 0.0 0 0 2 10 59 0.0 1 0 59 11 1450 0.0 1 4 1446 13 1 0.0 1 0 1 14 1 0.0 1 0 1 151 1 0.0 1 1 153 1 0.0 1 1 209 1 0.0 1 1 259 1 0.0 1 1 264 1 0.0 1 1 267 106 0.0 1 96 10 268 3 0.0 1 3 270 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.