This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.50 s (30 us/read; 2.01 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 56,649 (1.8%) Reads that were too short: 9,071 (0.3%) Reads written (passing filters): 47,578 (1.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 10,183,735 bp (1.1%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 56649 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2511 755.5 0 2511 7 10591 755.5 0 10591 8 41 755.5 0 41 9 5 755.5 0 5 10 4 755.5 0 4 11 8 755.5 0 8 12 10 755.5 0 10 13 5 755.5 0 5 14 7 755.5 0 7 15 10 755.5 0 10 16 9 755.5 0 9 17 11 755.5 0 11 18 18 755.5 0 18 19 20 755.5 0 20 20 31 755.5 0 31 21 8 755.5 0 8 22 11 755.5 0 11 23 9 755.5 0 9 24 10 755.5 0 10 25 2 755.5 0 2 26 7 755.5 0 7 27 6 755.5 0 6 28 11 755.5 0 11 29 12 755.5 0 12 30 18 755.5 0 18 31 6 755.5 0 6 32 14 755.5 0 14 33 12 755.5 0 12 34 10 755.5 0 10 35 9 755.5 0 9 36 14 755.5 0 14 37 9 755.5 0 9 38 10 755.5 0 10 39 11 755.5 0 11 40 11 755.5 0 11 41 10 755.5 0 10 42 8 755.5 0 8 43 15 755.5 0 15 44 28 755.5 0 28 45 15 755.5 0 15 46 16 755.5 0 16 47 17 755.5 0 17 48 139 755.5 0 139 49 11 755.5 0 11 50 13 755.5 0 13 51 25 755.5 0 25 52 21 755.5 0 21 53 30 755.5 0 30 54 26 755.5 0 26 55 12 755.5 0 12 56 14 755.5 0 14 57 11 755.5 0 11 58 14 755.5 0 14 59 13 755.5 0 13 60 18 755.5 0 18 61 12 755.5 0 12 62 18 755.5 0 18 63 16 755.5 0 16 64 25 755.5 0 25 65 27 755.5 0 27 66 44 755.5 0 44 67 146 755.5 0 146 68 84 755.5 0 84 69 168 755.5 0 168 70 871 755.5 0 871 71 308 755.5 0 308 72 51 755.5 0 51 73 59 755.5 0 59 74 73 755.5 0 73 75 116 755.5 0 116 76 89 755.5 0 89 77 105 755.5 0 105 78 132 755.5 0 132 79 278 755.5 0 278 80 2633 755.5 0 2633 81 5775 755.5 0 5775 82 8866 755.5 0 8866 83 58 755.5 0 58 84 25 755.5 0 25 85 9 755.5 0 9 86 9 755.5 0 9 87 16 755.5 0 16 88 9 755.5 0 9 89 37 755.5 0 37 90 16 755.5 0 16 91 29 755.5 0 29 92 35 755.5 0 35 93 24 755.5 0 24 94 38 755.5 0 38 95 47 755.5 0 47 96 20 755.5 0 20 97 180 755.5 0 180 98 28 755.5 0 28 99 11 755.5 0 11 100 11 755.5 0 11 101 26 755.5 0 26 102 11 755.5 0 11 103 12 755.5 0 12 104 16 755.5 0 16 105 9 755.5 0 9 106 11 755.5 0 11 107 13 755.5 0 13 108 5 755.5 0 5 109 4 755.5 0 4 110 7 755.5 0 7 111 27 755.5 0 27 112 12 755.5 0 12 113 12 755.5 0 12 114 5 755.5 0 5 115 9 755.5 0 9 116 85 755.5 0 85 117 58 755.5 0 58 118 64 755.5 0 64 119 209 755.5 0 209 120 60 755.5 0 60 121 59 755.5 0 59 122 47 755.5 0 47 123 16 755.5 0 16 124 19 755.5 0 19 125 18 755.5 0 18 126 34 755.5 0 34 127 175 755.5 0 175 128 22 755.5 0 22 129 28 755.5 0 28 130 18 755.5 0 18 131 22 755.5 0 22 132 30 755.5 0 30 133 23 755.5 0 23 134 25 755.5 0 25 135 22 755.5 0 22 136 21 755.5 0 21 137 29 755.5 0 29 138 26 755.5 0 26 139 32 755.5 0 32 140 17 755.5 0 17 141 14 755.5 0 14 142 139 755.5 0 139 143 37 755.5 0 37 144 15 755.5 0 15 145 124 755.5 0 124 146 26 755.5 0 26 147 16 755.5 0 16 148 20 755.5 0 20 149 21 755.5 0 21 150 54 755.5 0 54 151 8 755.5 0 8 152 24 755.5 0 24 153 18 755.5 0 18 154 15 755.5 0 15 155 22 755.5 0 22 156 23 755.5 0 23 157 28 755.5 0 28 158 26 755.5 0 26 159 20 755.5 0 20 160 21 755.5 0 21 161 22 755.5 0 22 162 37 755.5 0 37 163 29 755.5 0 29 164 26 755.5 0 26 165 24 755.5 0 24 166 70 755.5 0 70 167 88 755.5 0 88 168 59 755.5 0 59 169 27 755.5 0 27 170 25 755.5 0 25 171 44 755.5 0 44 172 11 755.5 0 11 173 29 755.5 0 29 174 40 755.5 0 40 175 75 755.5 0 75 176 24 755.5 0 24 177 45 755.5 0 45 178 74 755.5 0 74 179 35 755.5 0 35 180 127 755.5 0 127 181 66 755.5 0 66 182 62 755.5 0 62 183 79 755.5 0 79 184 64 755.5 0 64 185 54 755.5 0 54 186 59 755.5 0 59 187 119 755.5 0 119 188 1220 755.5 0 1220 189 1374 755.5 0 1374 190 4098 755.5 0 4098 191 421 755.5 0 421 192 294 755.5 0 294 193 630 755.5 0 630 194 616 755.5 0 616 195 679 755.5 0 679 196 222 755.5 0 222 197 25 755.5 0 25 198 17 755.5 0 17 199 22 755.5 0 22 200 35 755.5 0 35 201 67 755.5 0 67 202 21 755.5 0 21 203 28 755.5 0 28 204 48 755.5 0 48 205 61 755.5 0 61 206 93 755.5 0 93 207 198 755.5 0 198 208 422 755.5 0 422 209 299 755.5 0 299 210 31 755.5 0 31 211 99 755.5 0 99 212 237 755.5 0 237 213 250 755.5 0 250 214 92 755.5 0 92 215 34 755.5 0 34 216 38 755.5 0 38 217 28 755.5 0 28 218 24 755.5 0 24 219 19 755.5 0 19 220 22 755.5 0 22 221 56 755.5 0 56 222 33 755.5 0 33 223 24 755.5 0 24 224 49 755.5 0 49 225 44 755.5 0 44 226 39 755.5 0 39 227 32 755.5 0 32 228 35 755.5 0 35 229 34 755.5 0 34 230 29 755.5 0 29 231 33 755.5 0 33 232 49 755.5 0 49 233 27 755.5 0 27 234 21 755.5 0 21 235 35 755.5 0 35 236 43 755.5 0 43 237 52 755.5 0 52 238 25 755.5 0 25 239 25 755.5 0 25 240 36 755.5 0 36 241 44 755.5 0 44 242 74 755.5 0 74 243 13 755.5 0 13 244 27 755.5 0 27 245 27 755.5 0 27 246 16 755.5 0 16 247 12 755.5 0 12 248 31 755.5 0 31 249 15 755.5 0 15 250 37 755.5 0 37 251 27 755.5 0 27 252 21 755.5 0 21 253 43 755.5 0 43 254 20 755.5 0 20 255 43 755.5 0 43 256 49 755.5 0 49 257 47 755.5 0 47 258 38 755.5 0 38 259 24 755.5 0 24 260 44 755.5 0 44 261 21 755.5 0 21 262 33 755.5 0 33 263 40 755.5 0 40 264 40 755.5 0 40 265 32 755.5 0 32 266 28 755.5 0 28 267 20 755.5 0 20 268 45 755.5 0 45 269 74 755.5 0 74 270 101 755.5 0 101 271 94 755.5 0 94 272 766 755.5 0 766 273 799 755.5 0 799 274 242 755.5 0 242 275 16 755.5 0 16 276 37 755.5 0 37 277 19 755.5 0 19 278 17 755.5 0 17 279 16 755.5 0 16 280 28 755.5 0 28 281 60 755.5 0 60 282 66 755.5 0 66 283 93 755.5 0 93 284 111 755.5 0 111 285 18 755.5 0 18 286 27 755.5 0 27 287 21 755.5 0 21 288 34 755.5 0 34 289 33 755.5 0 33 290 72 755.5 0 72 291 618 755.5 0 618 292 929 755.5 0 929 293 253 755.5 0 253 294 283 755.5 0 283 295 90 755.5 0 90 296 28 755.5 0 28 297 60 755.5 0 60 298 90 755.5 0 90 299 85 755.5 0 85 300 197 755.5 0 197 301 18 755.5 0 18 Finished in 1.98 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 47,578 Reads with adapters: 13,092 (27.5%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 13,079 (27.5%) Total basepairs processed: 10,183,735 bp Total written (filtered): 3,613,906 bp (35.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13092 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 90 0.0 3 0 86 1 3 17 10574 0.0 3 10399 161 13 1 18 39 0.0 3 6 26 5 2 19 33 0.0 3 4 16 8 5 20 2302 0.0 3 2258 39 1 4 21 7 0.0 3 2 5 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 1 44 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 52 8 0.0 3 8 53 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 7 0.0 3 0 0 0 7 111 6 0.0 3 0 0 0 6 114 5 0.0 3 0 0 0 5 206 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 13,079 Reads with adapters: 1,202 (9.2%) Reads written (passing filters): 13,079 (100.0%) Total basepairs processed: 3,613,906 bp Total written (filtered): 3,610,294 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1202 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.6% C: 63.3% G: 18.1% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1200 204.4 0 1200 4 1 51.1 0 1 8 1 0.2 0 1