This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 92.66 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 128,940 (4.2%) Reads that were too short: 22,788 (0.7%) Reads written (passing filters): 106,152 (3.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 27,137,167 bp (2.9%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 128940 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 962 755.5 0 962 7 565 755.5 0 565 8 617 755.5 0 617 9 47026 755.5 0 47026 10 55 755.5 0 55 11 5 755.5 0 5 12 15 755.5 0 15 13 9 755.5 0 9 14 13 755.5 0 13 15 9 755.5 0 9 16 16 755.5 0 16 17 6 755.5 0 6 18 15 755.5 0 15 19 8 755.5 0 8 20 10 755.5 0 10 21 11 755.5 0 11 22 13 755.5 0 13 23 14 755.5 0 14 24 16 755.5 0 16 25 20 755.5 0 20 26 21 755.5 0 21 27 30 755.5 0 30 28 54 755.5 0 54 29 137 755.5 0 137 30 41 755.5 0 41 31 52 755.5 0 52 32 76 755.5 0 76 33 41 755.5 0 41 34 57 755.5 0 57 35 81 755.5 0 81 36 69 755.5 0 69 37 73 755.5 0 73 38 120 755.5 0 120 39 92 755.5 0 92 40 97 755.5 0 97 41 132 755.5 0 132 42 1658 755.5 0 1658 43 3638 755.5 0 3638 44 7185 755.5 0 7185 45 43 755.5 0 43 46 16 755.5 0 16 47 39 755.5 0 39 48 77 755.5 0 77 49 35 755.5 0 35 50 22 755.5 0 22 51 24 755.5 0 24 52 27 755.5 0 27 53 55 755.5 0 55 54 54 755.5 0 54 55 45 755.5 0 45 56 30 755.5 0 30 57 36 755.5 0 36 58 76 755.5 0 76 59 88 755.5 0 88 60 140 755.5 0 140 61 216 755.5 0 216 62 206 755.5 0 206 63 431 755.5 0 431 64 685 755.5 0 685 65 13321 755.5 0 13321 66 5290 755.5 0 5290 67 9959 755.5 0 9959 68 58 755.5 0 58 69 10 755.5 0 10 70 11 755.5 0 11 71 31 755.5 0 31 72 9 755.5 0 9 73 11 755.5 0 11 74 11 755.5 0 11 75 42 755.5 0 42 76 35 755.5 0 35 77 21 755.5 0 21 78 29 755.5 0 29 79 37 755.5 0 37 80 55 755.5 0 55 81 96 755.5 0 96 82 52 755.5 0 52 83 21 755.5 0 21 84 67 755.5 0 67 85 74 755.5 0 74 86 63 755.5 0 63 87 249 755.5 0 249 88 90 755.5 0 90 89 40 755.5 0 40 90 118 755.5 0 118 91 74 755.5 0 74 92 42 755.5 0 42 93 34 755.5 0 34 94 74 755.5 0 74 95 56 755.5 0 56 96 166 755.5 0 166 97 196 755.5 0 196 98 50 755.5 0 50 99 26 755.5 0 26 100 41 755.5 0 41 101 81 755.5 0 81 102 133 755.5 0 133 103 84 755.5 0 84 104 46 755.5 0 46 105 15 755.5 0 15 106 21 755.5 0 21 107 44 755.5 0 44 108 64 755.5 0 64 109 9 755.5 0 9 110 18 755.5 0 18 111 32 755.5 0 32 112 116 755.5 0 116 113 60 755.5 0 60 114 51 755.5 0 51 115 65 755.5 0 65 116 53 755.5 0 53 117 44 755.5 0 44 118 50 755.5 0 50 119 118 755.5 0 118 120 104 755.5 0 104 121 71 755.5 0 71 122 96 755.5 0 96 123 48 755.5 0 48 124 66 755.5 0 66 125 79 755.5 0 79 126 66 755.5 0 66 127 80 755.5 0 80 128 125 755.5 0 125 129 80 755.5 0 80 130 44 755.5 0 44 131 51 755.5 0 51 132 71 755.5 0 71 133 229 755.5 0 229 134 246 755.5 0 246 135 111 755.5 0 111 136 128 755.5 0 128 137 172 755.5 0 172 138 349 755.5 0 349 139 172 755.5 0 172 140 100 755.5 0 100 141 133 755.5 0 133 142 99 755.5 0 99 143 82 755.5 0 82 144 58 755.5 0 58 145 53 755.5 0 53 146 63 755.5 0 63 147 91 755.5 0 91 148 87 755.5 0 87 149 119 755.5 0 119 150 103 755.5 0 103 151 70 755.5 0 70 152 34 755.5 0 34 153 52 755.5 0 52 154 78 755.5 0 78 155 108 755.5 0 108 156 80 755.5 0 80 157 62 755.5 0 62 158 97 755.5 0 97 159 250 755.5 0 250 160 164 755.5 0 164 161 101 755.5 0 101 162 85 755.5 0 85 163 115 755.5 0 115 164 139 755.5 0 139 165 122 755.5 0 122 166 106 755.5 0 106 167 90 755.5 0 90 168 99 755.5 0 99 169 69 755.5 0 69 170 146 755.5 0 146 171 159 755.5 0 159 172 113 755.5 0 113 173 70 755.5 0 70 174 96 755.5 0 96 175 118 755.5 0 118 176 119 755.5 0 119 177 67 755.5 0 67 178 55 755.5 0 55 179 52 755.5 0 52 180 73 755.5 0 73 181 164 755.5 0 164 182 156 755.5 0 156 183 126 755.5 0 126 184 155 755.5 0 155 185 156 755.5 0 156 186 201 755.5 0 201 187 180 755.5 0 180 188 159 755.5 0 159 189 104 755.5 0 104 190 65 755.5 0 65 191 58 755.5 0 58 192 71 755.5 0 71 193 64 755.5 0 64 194 140 755.5 0 140 195 160 755.5 0 160 196 91 755.5 0 91 197 45 755.5 0 45 198 44 755.5 0 44 199 102 755.5 0 102 200 314 755.5 0 314 201 165 755.5 0 165 202 48 755.5 0 48 203 53 755.5 0 53 204 88 755.5 0 88 205 87 755.5 0 87 206 195 755.5 0 195 207 273 755.5 0 273 208 186 755.5 0 186 209 103 755.5 0 103 210 100 755.5 0 100 211 136 755.5 0 136 212 96 755.5 0 96 213 116 755.5 0 116 214 115 755.5 0 115 215 148 755.5 0 148 216 183 755.5 0 183 217 210 755.5 0 210 218 175 755.5 0 175 219 246 755.5 0 246 220 278 755.5 0 278 221 146 755.5 0 146 222 55 755.5 0 55 223 73 755.5 0 73 224 327 755.5 0 327 225 251 755.5 0 251 226 140 755.5 0 140 227 141 755.5 0 141 228 199 755.5 0 199 229 153 755.5 0 153 230 222 755.5 0 222 231 255 755.5 0 255 232 99 755.5 0 99 233 49 755.5 0 49 234 38 755.5 0 38 235 184 755.5 0 184 236 242 755.5 0 242 237 479 755.5 0 479 238 124 755.5 0 124 239 95 755.5 0 95 240 126 755.5 0 126 241 107 755.5 0 107 242 66 755.5 0 66 243 89 755.5 0 89 244 202 755.5 0 202 245 208 755.5 0 208 246 214 755.5 0 214 247 156 755.5 0 156 248 184 755.5 0 184 249 225 755.5 0 225 250 132 755.5 0 132 251 72 755.5 0 72 252 51 755.5 0 51 253 74 755.5 0 74 254 298 755.5 0 298 255 2579 755.5 0 2579 256 1549 755.5 0 1549 257 674 755.5 0 674 258 432 755.5 0 432 259 234 755.5 0 234 260 156 755.5 0 156 261 92 755.5 0 92 262 111 755.5 0 111 263 56 755.5 0 56 264 92 755.5 0 92 265 75 755.5 0 75 266 104 755.5 0 104 267 217 755.5 0 217 268 358 755.5 0 358 269 268 755.5 0 268 270 161 755.5 0 161 271 161 755.5 0 161 272 143 755.5 0 143 273 93 755.5 0 93 274 947 755.5 0 947 275 733 755.5 0 733 276 313 755.5 0 313 277 50 755.5 0 50 278 68 755.5 0 68 279 88 755.5 0 88 280 173 755.5 0 173 281 202 755.5 0 202 282 140 755.5 0 140 283 86 755.5 0 86 284 73 755.5 0 73 285 123 755.5 0 123 286 136 755.5 0 136 287 156 755.5 0 156 288 130 755.5 0 130 289 193 755.5 0 193 290 237 755.5 0 237 291 265 755.5 0 265 292 109 755.5 0 109 293 271 755.5 0 271 294 410 755.5 0 410 295 283 755.5 0 283 296 167 755.5 0 167 297 206 755.5 0 206 298 203 755.5 0 203 299 166 755.5 0 166 300 192 755.5 0 192 301 801 755.5 0 801 Finished in 5.83 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 106,152 Reads with adapters: 1,245 (1.2%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 1,216 (1.1%) Total basepairs processed: 27,137,167 bp Total written (filtered): 286,290 bp (1.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1245 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 6 0.0 4 5 0 0 1 22 9 0.0 4 2 7 23 798 0.0 4 775 19 3 1 24 2 0.0 4 0 1 0 0 1 34 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 76 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 103 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 0 2 113 4 0.0 4 0 0 0 0 4 114 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 269 0.0 4 0 0 0 0 269 134 2 0.0 4 0 0 0 0 2 135 108 0.0 4 0 0 0 0 108 136 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 155 1 0.0 4 0 0 0 0 1 193 9 0.0 4 0 0 0 0 9 194 3 0.0 4 0 0 0 0 3 198 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 1,216 Reads with adapters: 56 (4.6%) Reads written (passing filters): 1,216 (100.0%) Total basepairs processed: 286,290 bp Total written (filtered): 284,960 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 56 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.9% C: 3.6% G: 83.9% T: 3.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 19.0 0 2 9 13 0.0 0 0 13 10 11 0.0 1 0 11 11 27 0.0 1 0 27 261 1 0.0 1 1 269 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.