This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 96.05 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 401,769 (13.0%) Reads that were too short: 91,613 (3.0%) Reads written (passing filters): 310,156 (10.0%) Total basepairs processed: 931,490,553 bp Total written (filtered): 51,101,844 bp (5.5%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 401769 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 23 755.5 0 23 7 31 755.5 0 31 8 26 755.5 0 26 9 16 755.5 0 16 10 26 755.5 0 26 11 13 755.5 0 13 12 18 755.5 0 18 13 22 755.5 0 22 14 21 755.5 0 21 15 26 755.5 0 26 16 18 755.5 0 18 17 24 755.5 0 24 18 18 755.5 0 18 19 18 755.5 0 18 20 27 755.5 0 27 21 20 755.5 0 20 22 24 755.5 0 24 23 27 755.5 0 27 24 23 755.5 0 23 25 42 755.5 0 42 26 38 755.5 0 38 27 29 755.5 0 29 28 20 755.5 0 20 29 18 755.5 0 18 30 10 755.5 0 10 31 18 755.5 0 18 32 23 755.5 0 23 33 26 755.5 0 26 34 16 755.5 0 16 35 34 755.5 0 34 36 16 755.5 0 16 37 22 755.5 0 22 38 15 755.5 0 15 39 26 755.5 0 26 40 75 755.5 0 75 41 60 755.5 0 60 42 24 755.5 0 24 43 29 755.5 0 29 44 19 755.5 0 19 45 28 755.5 0 28 46 27 755.5 0 27 47 35 755.5 0 35 48 24 755.5 0 24 49 25 755.5 0 25 50 25 755.5 0 25 51 26 755.5 0 26 52 15 755.5 0 15 53 25 755.5 0 25 54 23 755.5 0 23 55 14 755.5 0 14 56 23 755.5 0 23 57 32 755.5 0 32 58 34 755.5 0 34 59 18 755.5 0 18 60 31 755.5 0 31 61 23 755.5 0 23 62 22 755.5 0 22 63 19 755.5 0 19 64 20 755.5 0 20 65 24 755.5 0 24 66 12 755.5 0 12 67 32 755.5 0 32 68 31 755.5 0 31 69 43 755.5 0 43 70 40 755.5 0 40 71 36 755.5 0 36 72 42 755.5 0 42 73 71 755.5 0 71 74 379 755.5 0 379 75 799 755.5 0 799 76 934 755.5 0 934 77 123 755.5 0 123 78 435 755.5 0 435 79 162 755.5 0 162 80 208 755.5 0 208 81 376 755.5 0 376 82 483 755.5 0 483 83 445 755.5 0 445 84 433 755.5 0 433 85 294 755.5 0 294 86 269 755.5 0 269 87 634 755.5 0 634 88 1044 755.5 0 1044 89 222 755.5 0 222 90 788 755.5 0 788 91 635 755.5 0 635 92 126 755.5 0 126 93 669 755.5 0 669 94 1231 755.5 0 1231 95 2199 755.5 0 2199 96 454 755.5 0 454 97 193 755.5 0 193 98 347 755.5 0 347 99 199 755.5 0 199 100 194 755.5 0 194 101 325 755.5 0 325 102 326 755.5 0 326 103 232 755.5 0 232 104 695 755.5 0 695 105 556 755.5 0 556 106 323 755.5 0 323 107 7811 755.5 0 7811 108 801 755.5 0 801 109 471 755.5 0 471 110 384 755.5 0 384 111 433 755.5 0 433 112 364 755.5 0 364 113 367 755.5 0 367 114 473 755.5 0 473 115 213 755.5 0 213 116 450 755.5 0 450 117 330 755.5 0 330 118 301 755.5 0 301 119 726 755.5 0 726 120 1698 755.5 0 1698 121 1364 755.5 0 1364 122 861 755.5 0 861 123 1086 755.5 0 1086 124 1033 755.5 0 1033 125 989 755.5 0 989 126 1370 755.5 0 1370 127 1540 755.5 0 1540 128 1309 755.5 0 1309 129 1298 755.5 0 1298 130 1365 755.5 0 1365 131 2020 755.5 0 2020 132 2444 755.5 0 2444 133 32625 755.5 0 32625 134 37241 755.5 0 37241 135 64432 755.5 0 64432 136 68599 755.5 0 68599 137 1011 755.5 0 1011 138 433 755.5 0 433 139 609 755.5 0 609 140 1100 755.5 0 1100 141 696 755.5 0 696 142 610 755.5 0 610 143 334 755.5 0 334 144 390 755.5 0 390 145 1268 755.5 0 1268 146 1019 755.5 0 1019 147 1767 755.5 0 1767 148 1014 755.5 0 1014 149 729 755.5 0 729 150 517 755.5 0 517 151 463 755.5 0 463 152 1411 755.5 0 1411 153 669 755.5 0 669 154 825 755.5 0 825 155 1329 755.5 0 1329 156 2134 755.5 0 2134 157 2868 755.5 0 2868 158 1697 755.5 0 1697 159 2351 755.5 0 2351 160 1255 755.5 0 1255 161 696 755.5 0 696 162 760 755.5 0 760 163 509 755.5 0 509 164 653 755.5 0 653 165 914 755.5 0 914 166 935 755.5 0 935 167 799 755.5 0 799 168 444 755.5 0 444 169 528 755.5 0 528 170 296 755.5 0 296 171 566 755.5 0 566 172 558 755.5 0 558 173 1563 755.5 0 1563 174 1864 755.5 0 1864 175 1179 755.5 0 1179 176 567 755.5 0 567 177 236 755.5 0 236 178 231 755.5 0 231 179 345 755.5 0 345 180 992 755.5 0 992 181 1198 755.5 0 1198 182 933 755.5 0 933 183 551 755.5 0 551 184 514 755.5 0 514 185 907 755.5 0 907 186 1642 755.5 0 1642 187 1076 755.5 0 1076 188 594 755.5 0 594 189 341 755.5 0 341 190 898 755.5 0 898 191 1380 755.5 0 1380 192 1144 755.5 0 1144 193 985 755.5 0 985 194 394 755.5 0 394 195 352 755.5 0 352 196 296 755.5 0 296 197 286 755.5 0 286 198 652 755.5 0 652 199 562 755.5 0 562 200 503 755.5 0 503 201 883 755.5 0 883 202 585 755.5 0 585 203 888 755.5 0 888 204 782 755.5 0 782 205 718 755.5 0 718 206 515 755.5 0 515 207 757 755.5 0 757 208 594 755.5 0 594 209 356 755.5 0 356 210 487 755.5 0 487 211 418 755.5 0 418 212 633 755.5 0 633 213 682 755.5 0 682 214 708 755.5 0 708 215 697 755.5 0 697 216 654 755.5 0 654 217 658 755.5 0 658 218 865 755.5 0 865 219 930 755.5 0 930 220 833 755.5 0 833 221 1081 755.5 0 1081 222 402 755.5 0 402 223 290 755.5 0 290 224 1215 755.5 0 1215 225 695 755.5 0 695 226 708 755.5 0 708 227 812 755.5 0 812 228 841 755.5 0 841 229 724 755.5 0 724 230 627 755.5 0 627 231 527 755.5 0 527 232 814 755.5 0 814 233 664 755.5 0 664 234 466 755.5 0 466 235 676 755.5 0 676 236 749 755.5 0 749 237 467 755.5 0 467 238 465 755.5 0 465 239 535 755.5 0 535 240 774 755.5 0 774 241 639 755.5 0 639 242 548 755.5 0 548 243 453 755.5 0 453 244 589 755.5 0 589 245 816 755.5 0 816 246 847 755.5 0 847 247 644 755.5 0 644 248 545 755.5 0 545 249 246 755.5 0 246 250 311 755.5 0 311 251 499 755.5 0 499 252 1420 755.5 0 1420 253 1260 755.5 0 1260 254 871 755.5 0 871 255 957 755.5 0 957 256 1359 755.5 0 1359 257 1091 755.5 0 1091 258 868 755.5 0 868 259 575 755.5 0 575 260 674 755.5 0 674 261 585 755.5 0 585 262 550 755.5 0 550 263 483 755.5 0 483 264 593 755.5 0 593 265 509 755.5 0 509 266 1047 755.5 0 1047 267 1007 755.5 0 1007 268 1370 755.5 0 1370 269 831 755.5 0 831 270 799 755.5 0 799 271 570 755.5 0 570 272 729 755.5 0 729 273 670 755.5 0 670 274 847 755.5 0 847 275 1611 755.5 0 1611 276 4232 755.5 0 4232 277 2968 755.5 0 2968 278 1376 755.5 0 1376 279 1596 755.5 0 1596 280 1218 755.5 0 1218 281 985 755.5 0 985 282 591 755.5 0 591 283 416 755.5 0 416 284 785 755.5 0 785 285 896 755.5 0 896 286 1199 755.5 0 1199 287 1032 755.5 0 1032 288 918 755.5 0 918 289 682 755.5 0 682 290 438 755.5 0 438 291 430 755.5 0 430 292 631 755.5 0 631 293 868 755.5 0 868 294 1499 755.5 0 1499 295 3879 755.5 0 3879 296 5333 755.5 0 5333 297 3724 755.5 0 3724 298 328 755.5 0 328 299 293 755.5 0 293 300 467 755.5 0 467 301 1124 755.5 0 1124 Finished in 10.79 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 310,156 Reads with adapters: 177 (0.1%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 148 (0.0%) Total basepairs processed: 51,101,844 bp Total written (filtered): 18,545 bp (0.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 177 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 2 17 2 0.0 3 2 18 2 0.0 3 0 0 1 1 20 1 0.0 3 1 32 49 0.0 3 0 0 0 49 35 10 0.0 3 0 0 1 9 40 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 42 0.0 3 0 0 0 42 59 10 0.0 3 0 0 0 10 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 92 38 0.0 3 0 0 0 38 93 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (68 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 148 Reads with adapters: 11 (7.4%) Reads written (passing filters): 148 (100.0%) Total basepairs processed: 18,545 bp Total written (filtered): 18,512 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 11 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.3% C: 9.1% G: 63.6% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 2.3 0 11