This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.53 s (29 us/read; 2.07 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 34,149 (1.1%) Reads that were too short: 10,520 (0.3%) Reads written (passing filters): 23,629 (0.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 4,794,920 bp (0.5%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 34149 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 94 755.5 0 94 7 190 755.5 0 190 8 9280 755.5 0 9280 9 40 755.5 0 40 10 6 755.5 0 6 12 4 755.5 0 4 13 1 755.5 0 1 15 2 755.5 0 2 16 4 755.5 0 4 17 4 755.5 0 4 18 12 755.5 0 12 19 11 755.5 0 11 20 10 755.5 0 10 21 6 755.5 0 6 22 3 755.5 0 3 23 4 755.5 0 4 24 3 755.5 0 3 25 4 755.5 0 4 26 1 755.5 0 1 27 3 755.5 0 3 28 5 755.5 0 5 29 3 755.5 0 3 30 3 755.5 0 3 31 6 755.5 0 6 32 1 755.5 0 1 33 2 755.5 0 2 34 4 755.5 0 4 35 2 755.5 0 2 36 5 755.5 0 5 37 2 755.5 0 2 38 3 755.5 0 3 39 1 755.5 0 1 40 1 755.5 0 1 41 4 755.5 0 4 42 1 755.5 0 1 43 5 755.5 0 5 44 19 755.5 0 19 45 11 755.5 0 11 46 14 755.5 0 14 47 6 755.5 0 6 48 5 755.5 0 5 49 1 755.5 0 1 50 7 755.5 0 7 51 4 755.5 0 4 52 5 755.5 0 5 53 1 755.5 0 1 54 7 755.5 0 7 55 7 755.5 0 7 56 16 755.5 0 16 57 8 755.5 0 8 58 3 755.5 0 3 59 3 755.5 0 3 60 9 755.5 0 9 61 4 755.5 0 4 62 6 755.5 0 6 64 3 755.5 0 3 65 7 755.5 0 7 66 2 755.5 0 2 67 4 755.5 0 4 68 14 755.5 0 14 69 9 755.5 0 9 70 18 755.5 0 18 71 26 755.5 0 26 72 12 755.5 0 12 73 16 755.5 0 16 74 12 755.5 0 12 75 34 755.5 0 34 76 130 755.5 0 130 77 654 755.5 0 654 78 560 755.5 0 560 79 205 755.5 0 205 80 15 755.5 0 15 81 12 755.5 0 12 82 6 755.5 0 6 83 3 755.5 0 3 84 11 755.5 0 11 85 2 755.5 0 2 86 1 755.5 0 1 87 3 755.5 0 3 88 14 755.5 0 14 90 3 755.5 0 3 91 16 755.5 0 16 92 49 755.5 0 49 93 22 755.5 0 22 94 28 755.5 0 28 95 15 755.5 0 15 96 8 755.5 0 8 97 21 755.5 0 21 98 40 755.5 0 40 99 9 755.5 0 9 100 10 755.5 0 10 101 5 755.5 0 5 102 12 755.5 0 12 103 10 755.5 0 10 104 12 755.5 0 12 105 10 755.5 0 10 106 2 755.5 0 2 107 8 755.5 0 8 108 7 755.5 0 7 109 6 755.5 0 6 110 2 755.5 0 2 111 4 755.5 0 4 112 8 755.5 0 8 113 18 755.5 0 18 114 35 755.5 0 35 115 32 755.5 0 32 116 64 755.5 0 64 117 69 755.5 0 69 118 10 755.5 0 10 119 6 755.5 0 6 120 11 755.5 0 11 121 6 755.5 0 6 122 19 755.5 0 19 123 44 755.5 0 44 124 15 755.5 0 15 125 14 755.5 0 14 126 8 755.5 0 8 127 11 755.5 0 11 128 23 755.5 0 23 129 35 755.5 0 35 130 15 755.5 0 15 131 5 755.5 0 5 132 10 755.5 0 10 133 13 755.5 0 13 134 34 755.5 0 34 135 108 755.5 0 108 136 88 755.5 0 88 137 62 755.5 0 62 138 227 755.5 0 227 139 26 755.5 0 26 140 10 755.5 0 10 141 56 755.5 0 56 142 20 755.5 0 20 143 68 755.5 0 68 144 18 755.5 0 18 145 3 755.5 0 3 146 8 755.5 0 8 147 9 755.5 0 9 148 39 755.5 0 39 149 13 755.5 0 13 150 84 755.5 0 84 151 13 755.5 0 13 152 21 755.5 0 21 153 2 755.5 0 2 154 13 755.5 0 13 155 29 755.5 0 29 156 20 755.5 0 20 157 19 755.5 0 19 158 35 755.5 0 35 159 10 755.5 0 10 160 18 755.5 0 18 161 10 755.5 0 10 162 29 755.5 0 29 163 122 755.5 0 122 164 57 755.5 0 57 165 45 755.5 0 45 166 22 755.5 0 22 167 30 755.5 0 30 168 28 755.5 0 28 169 98 755.5 0 98 170 20 755.5 0 20 171 71 755.5 0 71 172 64 755.5 0 64 173 49 755.5 0 49 174 212 755.5 0 212 175 3918 755.5 0 3918 176 113 755.5 0 113 177 45 755.5 0 45 178 60 755.5 0 60 179 58 755.5 0 58 180 238 755.5 0 238 181 63 755.5 0 63 182 75 755.5 0 75 183 36 755.5 0 36 184 39 755.5 0 39 185 19 755.5 0 19 186 31 755.5 0 31 187 43 755.5 0 43 188 71 755.5 0 71 189 132 755.5 0 132 190 240 755.5 0 240 191 1495 755.5 0 1495 192 474 755.5 0 474 193 1809 755.5 0 1809 194 50 755.5 0 50 195 26 755.5 0 26 196 42 755.5 0 42 197 27 755.5 0 27 198 19 755.5 0 19 199 28 755.5 0 28 200 47 755.5 0 47 201 112 755.5 0 112 202 863 755.5 0 863 203 133 755.5 0 133 204 77 755.5 0 77 205 53 755.5 0 53 206 8 755.5 0 8 207 15 755.5 0 15 208 37 755.5 0 37 209 39 755.5 0 39 210 38 755.5 0 38 211 24 755.5 0 24 212 11 755.5 0 11 213 10 755.5 0 10 214 6 755.5 0 6 215 20 755.5 0 20 216 16 755.5 0 16 217 12 755.5 0 12 218 15 755.5 0 15 219 7 755.5 0 7 220 14 755.5 0 14 221 24 755.5 0 24 222 4 755.5 0 4 223 8 755.5 0 8 224 1 755.5 0 1 225 23 755.5 0 23 226 22 755.5 0 22 227 18 755.5 0 18 228 17 755.5 0 17 229 20 755.5 0 20 230 5 755.5 0 5 231 8 755.5 0 8 232 3 755.5 0 3 233 30 755.5 0 30 234 19 755.5 0 19 235 6 755.5 0 6 236 12 755.5 0 12 237 14 755.5 0 14 238 10 755.5 0 10 239 5 755.5 0 5 240 6 755.5 0 6 241 12 755.5 0 12 242 7 755.5 0 7 243 4 755.5 0 4 244 3 755.5 0 3 245 6 755.5 0 6 246 11 755.5 0 11 247 5 755.5 0 5 248 9 755.5 0 9 249 12 755.5 0 12 250 22 755.5 0 22 251 16 755.5 0 16 252 18 755.5 0 18 253 30 755.5 0 30 254 81 755.5 0 81 255 21 755.5 0 21 256 11 755.5 0 11 257 11 755.5 0 11 258 22 755.5 0 22 259 134 755.5 0 134 260 281 755.5 0 281 261 197 755.5 0 197 262 111 755.5 0 111 263 19 755.5 0 19 264 38 755.5 0 38 265 52 755.5 0 52 266 161 755.5 0 161 267 13 755.5 0 13 268 12 755.5 0 12 269 44 755.5 0 44 270 61 755.5 0 61 271 64 755.5 0 64 272 24 755.5 0 24 273 8 755.5 0 8 274 17 755.5 0 17 275 30 755.5 0 30 276 58 755.5 0 58 277 110 755.5 0 110 278 63 755.5 0 63 279 8 755.5 0 8 280 27 755.5 0 27 281 76 755.5 0 76 282 46 755.5 0 46 283 37 755.5 0 37 284 106 755.5 0 106 285 315 755.5 0 315 286 2101 755.5 0 2101 287 48 755.5 0 48 288 158 755.5 0 158 289 2030 755.5 0 2030 290 629 755.5 0 629 291 144 755.5 0 144 292 10 755.5 0 10 293 6 755.5 0 6 294 4 755.5 0 4 295 96 755.5 0 96 296 256 755.5 0 256 297 935 755.5 0 935 298 20 755.5 0 20 299 10 755.5 0 10 300 1 755.5 0 1 301 6 755.5 0 6 Finished in 0.94 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 23,629 Reads with adapters: 9,536 (40.4%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 9,535 (40.4%) Total basepairs processed: 4,794,920 bp Total written (filtered): 2,628,426 bp (54.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9536 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 6 1 16 81 0.0 3 0 74 5 2 17 9306 0.0 3 9158 142 5 1 18 53 0.0 3 4 26 15 8 19 60 0.0 3 2 0 32 26 20 12 0.0 3 1 0 0 11 21 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 81 9 0.0 3 8 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 9,535 Reads with adapters: 93 (1.0%) Reads written (passing filters): 9,535 (100.0%) Total basepairs processed: 2,628,426 bp Total written (filtered): 2,628,144 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 93 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.8% C: 51.6% G: 4.3% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 91 149.0 0 91 4 1 37.2 0 1 5 1 9.3 0 1