This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.83 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 14,797 (0.5%) Reads that were too short: 2,674 (0.1%) Reads written (passing filters): 12,123 (0.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 2,652,080 bp (0.3%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 14797 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 88 755.5 0 88 7 1663 755.5 0 1663 8 2601 755.5 0 2601 9 46 755.5 0 46 10 26 755.5 0 26 11 15 755.5 0 15 12 41 755.5 0 41 13 6 755.5 0 6 14 11 755.5 0 11 15 10 755.5 0 10 16 10 755.5 0 10 17 10 755.5 0 10 18 4 755.5 0 4 19 8 755.5 0 8 20 17 755.5 0 17 21 8 755.5 0 8 22 25 755.5 0 25 23 11 755.5 0 11 24 7 755.5 0 7 25 6 755.5 0 6 26 9 755.5 0 9 27 10 755.5 0 10 28 5 755.5 0 5 29 4 755.5 0 4 30 9 755.5 0 9 31 3 755.5 0 3 32 10 755.5 0 10 33 10 755.5 0 10 34 12 755.5 0 12 35 6 755.5 0 6 36 12 755.5 0 12 37 13 755.5 0 13 38 22 755.5 0 22 39 4 755.5 0 4 40 22 755.5 0 22 41 9 755.5 0 9 42 6 755.5 0 6 43 12 755.5 0 12 44 9 755.5 0 9 45 13 755.5 0 13 46 14 755.5 0 14 47 70 755.5 0 70 48 684 755.5 0 684 49 42 755.5 0 42 50 10 755.5 0 10 51 9 755.5 0 9 52 12 755.5 0 12 53 8 755.5 0 8 54 23 755.5 0 23 55 8 755.5 0 8 56 7 755.5 0 7 57 9 755.5 0 9 58 7 755.5 0 7 59 14 755.5 0 14 60 30 755.5 0 30 61 13 755.5 0 13 62 17 755.5 0 17 63 7 755.5 0 7 64 9 755.5 0 9 65 20 755.5 0 20 66 7 755.5 0 7 67 11 755.5 0 11 68 16 755.5 0 16 69 90 755.5 0 90 70 36 755.5 0 36 71 97 755.5 0 97 72 123 755.5 0 123 73 77 755.5 0 77 74 56 755.5 0 56 75 16 755.5 0 16 76 6 755.5 0 6 77 3 755.5 0 3 78 6 755.5 0 6 79 11 755.5 0 11 80 24 755.5 0 24 81 59 755.5 0 59 82 28 755.5 0 28 83 50 755.5 0 50 84 421 755.5 0 421 85 122 755.5 0 122 86 101 755.5 0 101 87 15 755.5 0 15 88 15 755.5 0 15 89 6 755.5 0 6 90 5 755.5 0 5 91 9 755.5 0 9 92 7 755.5 0 7 93 8 755.5 0 8 94 7 755.5 0 7 95 8 755.5 0 8 96 6 755.5 0 6 97 10 755.5 0 10 98 10 755.5 0 10 99 12 755.5 0 12 100 11 755.5 0 11 101 7 755.5 0 7 102 12 755.5 0 12 103 8 755.5 0 8 104 5 755.5 0 5 105 10 755.5 0 10 106 3 755.5 0 3 107 10 755.5 0 10 108 22 755.5 0 22 109 25 755.5 0 25 110 111 755.5 0 111 111 230 755.5 0 230 112 105 755.5 0 105 113 6 755.5 0 6 114 4 755.5 0 4 115 6 755.5 0 6 116 9 755.5 0 9 117 15 755.5 0 15 118 5 755.5 0 5 119 9 755.5 0 9 120 10 755.5 0 10 121 9 755.5 0 9 122 8 755.5 0 8 123 10 755.5 0 10 124 17 755.5 0 17 125 14 755.5 0 14 126 5 755.5 0 5 127 9 755.5 0 9 128 7 755.5 0 7 129 10 755.5 0 10 130 23 755.5 0 23 131 51 755.5 0 51 132 287 755.5 0 287 133 14 755.5 0 14 134 8 755.5 0 8 135 11 755.5 0 11 136 14 755.5 0 14 137 19 755.5 0 19 138 16 755.5 0 16 139 10 755.5 0 10 140 12 755.5 0 12 141 10 755.5 0 10 142 8 755.5 0 8 143 7 755.5 0 7 144 8 755.5 0 8 145 10 755.5 0 10 146 10 755.5 0 10 147 12 755.5 0 12 148 69 755.5 0 69 149 18 755.5 0 18 150 16 755.5 0 16 151 9 755.5 0 9 152 10 755.5 0 10 153 5 755.5 0 5 154 4 755.5 0 4 155 10 755.5 0 10 156 28 755.5 0 28 157 27 755.5 0 27 158 27 755.5 0 27 159 13 755.5 0 13 160 17 755.5 0 17 161 12 755.5 0 12 162 16 755.5 0 16 163 25 755.5 0 25 164 22 755.5 0 22 165 25 755.5 0 25 166 44 755.5 0 44 167 35 755.5 0 35 168 70 755.5 0 70 169 99 755.5 0 99 170 1443 755.5 0 1443 171 16 755.5 0 16 172 10 755.5 0 10 173 10 755.5 0 10 174 9 755.5 0 9 175 9 755.5 0 9 176 71 755.5 0 71 177 21 755.5 0 21 178 30 755.5 0 30 179 12 755.5 0 12 180 9 755.5 0 9 181 18 755.5 0 18 182 389 755.5 0 389 183 16 755.5 0 16 184 28 755.5 0 28 185 7 755.5 0 7 186 13 755.5 0 13 187 17 755.5 0 17 188 23 755.5 0 23 189 23 755.5 0 23 190 8 755.5 0 8 191 8 755.5 0 8 192 15 755.5 0 15 193 13 755.5 0 13 194 26 755.5 0 26 195 34 755.5 0 34 196 105 755.5 0 105 197 79 755.5 0 79 198 218 755.5 0 218 199 266 755.5 0 266 200 17 755.5 0 17 201 13 755.5 0 13 202 14 755.5 0 14 203 60 755.5 0 60 204 122 755.5 0 122 205 160 755.5 0 160 206 30 755.5 0 30 207 14 755.5 0 14 208 15 755.5 0 15 209 7 755.5 0 7 210 10 755.5 0 10 211 18 755.5 0 18 212 16 755.5 0 16 213 49 755.5 0 49 214 181 755.5 0 181 215 231 755.5 0 231 216 37 755.5 0 37 217 15 755.5 0 15 218 14 755.5 0 14 219 17 755.5 0 17 220 5 755.5 0 5 221 14 755.5 0 14 222 43 755.5 0 43 223 49 755.5 0 49 224 92 755.5 0 92 225 117 755.5 0 117 226 11 755.5 0 11 227 10 755.5 0 10 228 9 755.5 0 9 229 15 755.5 0 15 230 2 755.5 0 2 231 9 755.5 0 9 232 3 755.5 0 3 233 8 755.5 0 8 234 6 755.5 0 6 235 12 755.5 0 12 236 44 755.5 0 44 237 7 755.5 0 7 238 7 755.5 0 7 239 7 755.5 0 7 240 6 755.5 0 6 241 5 755.5 0 5 242 21 755.5 0 21 243 62 755.5 0 62 244 19 755.5 0 19 245 15 755.5 0 15 246 26 755.5 0 26 247 13 755.5 0 13 248 7 755.5 0 7 249 10 755.5 0 10 250 6 755.5 0 6 251 12 755.5 0 12 252 47 755.5 0 47 253 60 755.5 0 60 254 23 755.5 0 23 255 27 755.5 0 27 256 8 755.5 0 8 257 6 755.5 0 6 258 21 755.5 0 21 259 39 755.5 0 39 260 166 755.5 0 166 261 13 755.5 0 13 262 15 755.5 0 15 263 13 755.5 0 13 264 8 755.5 0 8 265 11 755.5 0 11 266 3 755.5 0 3 267 7 755.5 0 7 268 13 755.5 0 13 269 7 755.5 0 7 270 17 755.5 0 17 271 22 755.5 0 22 272 10 755.5 0 10 273 10 755.5 0 10 274 5 755.5 0 5 275 4 755.5 0 4 276 8 755.5 0 8 277 9 755.5 0 9 278 17 755.5 0 17 279 8 755.5 0 8 280 12 755.5 0 12 281 19 755.5 0 19 282 33 755.5 0 33 283 36 755.5 0 36 284 45 755.5 0 45 285 18 755.5 0 18 286 3 755.5 0 3 287 1 755.5 0 1 288 7 755.5 0 7 289 16 755.5 0 16 290 10 755.5 0 10 291 8 755.5 0 8 292 6 755.5 0 6 293 5 755.5 0 5 294 21 755.5 0 21 295 10 755.5 0 10 296 31 755.5 0 31 297 39 755.5 0 39 298 23 755.5 0 23 299 17 755.5 0 17 300 16 755.5 0 16 301 19 755.5 0 19 Finished in 0.61 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 12,123 Reads with adapters: 2,756 (22.7%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 2,752 (22.7%) Total basepairs processed: 2,652,080 bp Total written (filtered): 746,631 bp (28.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2756 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 2 19 34 0.0 3 0 30 3 0 1 20 2661 0.0 4 2558 90 10 2 1 21 4 0.0 4 1 3 22 2 0.0 4 0 0 1 0 1 23 3 0.0 4 0 0 0 3 98 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 44 0.0 4 0 0 0 0 44 149 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 2,752 Reads with adapters: 378 (13.7%) Reads written (passing filters): 2,752 (100.0%) Total basepairs processed: 746,631 bp Total written (filtered): 716,435 bp (96.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 378 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 28.8% C: 1.3% G: 68.5% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 4 10.8 0 4 10 5 0.0 1 1 4 11 263 0.0 1 0 263 12 1 0.0 1 0 1 257 1 0.0 1 1 258 1 0.0 1 1 259 90 0.0 1 82 8 260 6 0.0 1 6 261 4 0.0 1 4 264 2 0.0 1 2 268 1 0.0 1 1