This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.80 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 28,800 (0.9%) Reads that were too short: 15,574 (0.5%) Reads written (passing filters): 13,226 (0.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 2,515,997 bp (0.3%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 28800 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 38 755.5 0 38 7 2666 755.5 0 2666 8 20 755.5 0 20 9 4 755.5 0 4 10 8 755.5 0 8 11 4 755.5 0 4 12 3 755.5 0 3 13 4 755.5 0 4 14 5 755.5 0 5 15 7 755.5 0 7 16 1 755.5 0 1 17 5 755.5 0 5 18 29 755.5 0 29 19 8 755.5 0 8 20 10 755.5 0 10 21 7 755.5 0 7 22 2 755.5 0 2 23 2 755.5 0 2 24 5 755.5 0 5 25 4 755.5 0 4 26 8 755.5 0 8 27 4 755.5 0 4 28 6 755.5 0 6 29 5 755.5 0 5 30 4 755.5 0 4 31 5 755.5 0 5 32 6 755.5 0 6 33 8 755.5 0 8 34 3 755.5 0 3 35 8 755.5 0 8 36 44 755.5 0 44 37 31 755.5 0 31 38 215 755.5 0 215 39 4 755.5 0 4 40 2 755.5 0 2 41 8 755.5 0 8 42 21 755.5 0 21 43 27 755.5 0 27 44 87 755.5 0 87 45 145 755.5 0 145 46 4 755.5 0 4 47 4 755.5 0 4 48 4 755.5 0 4 49 5 755.5 0 5 50 5 755.5 0 5 51 5 755.5 0 5 52 5 755.5 0 5 53 5 755.5 0 5 54 5 755.5 0 5 55 4 755.5 0 4 56 2 755.5 0 2 57 5 755.5 0 5 58 3 755.5 0 3 59 2 755.5 0 2 60 5 755.5 0 5 61 3 755.5 0 3 62 4 755.5 0 4 64 2 755.5 0 2 65 4 755.5 0 4 66 5 755.5 0 5 67 3 755.5 0 3 68 6 755.5 0 6 69 2 755.5 0 2 70 5 755.5 0 5 71 2 755.5 0 2 72 9 755.5 0 9 73 1 755.5 0 1 74 2 755.5 0 2 75 7 755.5 0 7 76 3 755.5 0 3 77 6 755.5 0 6 78 16 755.5 0 16 79 34 755.5 0 34 80 14 755.5 0 14 81 12 755.5 0 12 82 13 755.5 0 13 83 17 755.5 0 17 84 12 755.5 0 12 85 5 755.5 0 5 86 6 755.5 0 6 87 14 755.5 0 14 88 14 755.5 0 14 89 6 755.5 0 6 90 5 755.5 0 5 91 34 755.5 0 34 92 22 755.5 0 22 93 79 755.5 0 79 94 278 755.5 0 278 95 55 755.5 0 55 96 35 755.5 0 35 97 72 755.5 0 72 98 49 755.5 0 49 99 145 755.5 0 145 100 27 755.5 0 27 101 20 755.5 0 20 102 6 755.5 0 6 103 10 755.5 0 10 104 27 755.5 0 27 105 13 755.5 0 13 106 10 755.5 0 10 107 18 755.5 0 18 108 24 755.5 0 24 109 25 755.5 0 25 110 20 755.5 0 20 111 16 755.5 0 16 112 16 755.5 0 16 113 32 755.5 0 32 114 19 755.5 0 19 115 30 755.5 0 30 116 43 755.5 0 43 117 70 755.5 0 70 118 19 755.5 0 19 119 39 755.5 0 39 120 114 755.5 0 114 121 78 755.5 0 78 122 70 755.5 0 70 123 59 755.5 0 59 124 47 755.5 0 47 125 51 755.5 0 51 126 47 755.5 0 47 127 31 755.5 0 31 128 124 755.5 0 124 129 109 755.5 0 109 130 122 755.5 0 122 131 65 755.5 0 65 132 74 755.5 0 74 133 85 755.5 0 85 134 287 755.5 0 287 135 1181 755.5 0 1181 136 530 755.5 0 530 137 622 755.5 0 622 138 82 755.5 0 82 139 64 755.5 0 64 140 77 755.5 0 77 141 53 755.5 0 53 142 65 755.5 0 65 143 159 755.5 0 159 144 146 755.5 0 146 145 78 755.5 0 78 146 53 755.5 0 53 147 33 755.5 0 33 148 36 755.5 0 36 149 32 755.5 0 32 150 64 755.5 0 64 151 50 755.5 0 50 152 19 755.5 0 19 153 30 755.5 0 30 154 31 755.5 0 31 155 64 755.5 0 64 156 75 755.5 0 75 157 55 755.5 0 55 158 55 755.5 0 55 159 36 755.5 0 36 160 35 755.5 0 35 161 43 755.5 0 43 162 38 755.5 0 38 163 76 755.5 0 76 164 59 755.5 0 59 165 94 755.5 0 94 166 94 755.5 0 94 167 102 755.5 0 102 168 107 755.5 0 107 169 32 755.5 0 32 170 30 755.5 0 30 171 32 755.5 0 32 172 55 755.5 0 55 173 52 755.5 0 52 174 47 755.5 0 47 175 24 755.5 0 24 176 19 755.5 0 19 177 18 755.5 0 18 178 47 755.5 0 47 179 65 755.5 0 65 180 109 755.5 0 109 181 101 755.5 0 101 182 88 755.5 0 88 183 73 755.5 0 73 184 89 755.5 0 89 185 65 755.5 0 65 186 53 755.5 0 53 187 16 755.5 0 16 188 30 755.5 0 30 189 66 755.5 0 66 190 180 755.5 0 180 191 133 755.5 0 133 192 101 755.5 0 101 193 66 755.5 0 66 194 56 755.5 0 56 195 67 755.5 0 67 196 365 755.5 0 365 197 170 755.5 0 170 198 60 755.5 0 60 199 22 755.5 0 22 200 20 755.5 0 20 201 153 755.5 0 153 202 482 755.5 0 482 203 547 755.5 0 547 204 242 755.5 0 242 205 70 755.5 0 70 206 43 755.5 0 43 207 156 755.5 0 156 208 205 755.5 0 205 209 445 755.5 0 445 210 164 755.5 0 164 211 185 755.5 0 185 212 185 755.5 0 185 213 171 755.5 0 171 214 97 755.5 0 97 215 149 755.5 0 149 216 143 755.5 0 143 217 95 755.5 0 95 218 67 755.5 0 67 219 58 755.5 0 58 220 87 755.5 0 87 221 90 755.5 0 90 222 264 755.5 0 264 223 102 755.5 0 102 224 149 755.5 0 149 225 140 755.5 0 140 226 130 755.5 0 130 227 103 755.5 0 103 228 78 755.5 0 78 229 52 755.5 0 52 230 38 755.5 0 38 231 23 755.5 0 23 232 43 755.5 0 43 233 79 755.5 0 79 234 93 755.5 0 93 235 180 755.5 0 180 236 161 755.5 0 161 237 60 755.5 0 60 238 34 755.5 0 34 239 24 755.5 0 24 240 19 755.5 0 19 241 55 755.5 0 55 242 107 755.5 0 107 243 167 755.5 0 167 244 180 755.5 0 180 245 92 755.5 0 92 246 38 755.5 0 38 247 21 755.5 0 21 248 21 755.5 0 21 249 32 755.5 0 32 250 43 755.5 0 43 251 166 755.5 0 166 252 88 755.5 0 88 253 115 755.5 0 115 254 79 755.5 0 79 255 89 755.5 0 89 256 92 755.5 0 92 257 56 755.5 0 56 258 33 755.5 0 33 259 49 755.5 0 49 260 77 755.5 0 77 261 148 755.5 0 148 262 121 755.5 0 121 263 96 755.5 0 96 264 121 755.5 0 121 265 121 755.5 0 121 266 107 755.5 0 107 267 49 755.5 0 49 268 22 755.5 0 22 269 129 755.5 0 129 270 160 755.5 0 160 271 225 755.5 0 225 272 167 755.5 0 167 273 125 755.5 0 125 274 89 755.5 0 89 275 81 755.5 0 81 276 74 755.5 0 74 277 92 755.5 0 92 278 120 755.5 0 120 279 885 755.5 0 885 280 252 755.5 0 252 281 223 755.5 0 223 282 145 755.5 0 145 283 103 755.5 0 103 284 167 755.5 0 167 285 104 755.5 0 104 286 74 755.5 0 74 287 49 755.5 0 49 288 22 755.5 0 22 289 74 755.5 0 74 290 91 755.5 0 91 291 166 755.5 0 166 292 187 755.5 0 187 293 65 755.5 0 65 294 107 755.5 0 107 295 342 755.5 0 342 296 1453 755.5 0 1453 297 260 755.5 0 260 298 843 755.5 0 843 299 373 755.5 0 373 300 304 755.5 0 304 301 185 755.5 0 185 Finished in 0.54 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 13,226 Reads with adapters: 2,695 (20.4%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 2,690 (20.3%) Total basepairs processed: 2,515,997 bp Total written (filtered): 744,304 bp (29.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2695 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 2 0.0 3 0 0 1 1 16 25 0.0 3 0 25 17 2640 0.0 3 2587 50 3 18 10 0.0 3 1 8 0 1 19 5 0.0 3 1 0 1 3 46 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 110 2 0.0 3 1 0 0 1 130 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 2,690 Reads with adapters: 173 (6.4%) Reads written (passing filters): 2,690 (100.0%) Total basepairs processed: 744,304 bp Total written (filtered): 743,785 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 173 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.4% C: 73.4% G: 19.1% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 173 42.0 0 173