This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 93.05 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 145,805 (4.7%) Reads that were too short: 97,323 (3.1%) Reads written (passing filters): 48,482 (1.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 9,130,852 bp (1.0%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 145805 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 579 755.5 0 579 7 3913 755.5 0 3913 8 85 755.5 0 85 9 5350 755.5 0 5350 10 13 755.5 0 13 11 11 755.5 0 11 12 11 755.5 0 11 13 9 755.5 0 9 14 103 755.5 0 103 15 8 755.5 0 8 16 7 755.5 0 7 17 32 755.5 0 32 18 12 755.5 0 12 19 4 755.5 0 4 20 5 755.5 0 5 21 8 755.5 0 8 22 4 755.5 0 4 23 5 755.5 0 5 24 11 755.5 0 11 25 11 755.5 0 11 26 9 755.5 0 9 27 8 755.5 0 8 28 8 755.5 0 8 29 16 755.5 0 16 30 7 755.5 0 7 31 17 755.5 0 17 32 8 755.5 0 8 33 6 755.5 0 6 34 8 755.5 0 8 35 11 755.5 0 11 36 23 755.5 0 23 37 16 755.5 0 16 38 30 755.5 0 30 39 8 755.5 0 8 40 8 755.5 0 8 41 8 755.5 0 8 42 8 755.5 0 8 43 3 755.5 0 3 44 15 755.5 0 15 45 6 755.5 0 6 46 8 755.5 0 8 47 9 755.5 0 9 48 10 755.5 0 10 49 11 755.5 0 11 50 20 755.5 0 20 51 9 755.5 0 9 52 21 755.5 0 21 53 11 755.5 0 11 54 14 755.5 0 14 55 6 755.5 0 6 56 106 755.5 0 106 57 122 755.5 0 122 58 80 755.5 0 80 59 7 755.5 0 7 60 8 755.5 0 8 61 18 755.5 0 18 62 26 755.5 0 26 63 13 755.5 0 13 64 11 755.5 0 11 65 4 755.5 0 4 66 12 755.5 0 12 67 13 755.5 0 13 68 6 755.5 0 6 69 7 755.5 0 7 70 12 755.5 0 12 71 58 755.5 0 58 72 24 755.5 0 24 73 29 755.5 0 29 74 20 755.5 0 20 75 15 755.5 0 15 76 65 755.5 0 65 77 35 755.5 0 35 78 51 755.5 0 51 79 126 755.5 0 126 80 76 755.5 0 76 81 89 755.5 0 89 82 135 755.5 0 135 83 241 755.5 0 241 84 90 755.5 0 90 85 160 755.5 0 160 86 135 755.5 0 135 87 695 755.5 0 695 88 200 755.5 0 200 89 756 755.5 0 756 90 233 755.5 0 233 91 196 755.5 0 196 92 127 755.5 0 127 93 127 755.5 0 127 94 115 755.5 0 115 95 46 755.5 0 46 96 26 755.5 0 26 97 69 755.5 0 69 98 68 755.5 0 68 99 121 755.5 0 121 100 359 755.5 0 359 101 110 755.5 0 110 102 112 755.5 0 112 103 250 755.5 0 250 104 263 755.5 0 263 105 300 755.5 0 300 106 132 755.5 0 132 107 50 755.5 0 50 108 93 755.5 0 93 109 214 755.5 0 214 110 38 755.5 0 38 111 57 755.5 0 57 112 116 755.5 0 116 113 127 755.5 0 127 114 78 755.5 0 78 115 82 755.5 0 82 116 110 755.5 0 110 117 186 755.5 0 186 118 115 755.5 0 115 119 144 755.5 0 144 120 225 755.5 0 225 121 226 755.5 0 226 122 271 755.5 0 271 123 86 755.5 0 86 124 70 755.5 0 70 125 105 755.5 0 105 126 129 755.5 0 129 127 64 755.5 0 64 128 76 755.5 0 76 129 134 755.5 0 134 130 141 755.5 0 141 131 165 755.5 0 165 132 91 755.5 0 91 133 172 755.5 0 172 134 287 755.5 0 287 135 373 755.5 0 373 136 109 755.5 0 109 137 156 755.5 0 156 138 208 755.5 0 208 139 213 755.5 0 213 140 1501 755.5 0 1501 141 598 755.5 0 598 142 574 755.5 0 574 143 143 755.5 0 143 144 312 755.5 0 312 145 360 755.5 0 360 146 5305 755.5 0 5305 147 1888 755.5 0 1888 148 1282 755.5 0 1282 149 203 755.5 0 203 150 2065 755.5 0 2065 151 979 755.5 0 979 152 1960 755.5 0 1960 153 142 755.5 0 142 154 144 755.5 0 144 155 129 755.5 0 129 156 1554 755.5 0 1554 157 434 755.5 0 434 158 525 755.5 0 525 159 182 755.5 0 182 160 354 755.5 0 354 161 299 755.5 0 299 162 256 755.5 0 256 163 138 755.5 0 138 164 188 755.5 0 188 165 179 755.5 0 179 166 250 755.5 0 250 167 179 755.5 0 179 168 225 755.5 0 225 169 144 755.5 0 144 170 104 755.5 0 104 171 48 755.5 0 48 172 62 755.5 0 62 173 125 755.5 0 125 174 45 755.5 0 45 175 65 755.5 0 65 176 69 755.5 0 69 177 91 755.5 0 91 178 120 755.5 0 120 179 186 755.5 0 186 180 205 755.5 0 205 181 300 755.5 0 300 182 460 755.5 0 460 183 323 755.5 0 323 184 281 755.5 0 281 185 298 755.5 0 298 186 323 755.5 0 323 187 75 755.5 0 75 188 109 755.5 0 109 189 119 755.5 0 119 190 132 755.5 0 132 191 221 755.5 0 221 192 73 755.5 0 73 193 76 755.5 0 76 194 72 755.5 0 72 195 68 755.5 0 68 196 79 755.5 0 79 197 199 755.5 0 199 198 116 755.5 0 116 199 45 755.5 0 45 200 39 755.5 0 39 201 129 755.5 0 129 202 437 755.5 0 437 203 367 755.5 0 367 204 257 755.5 0 257 205 269 755.5 0 269 206 197 755.5 0 197 207 480 755.5 0 480 208 661 755.5 0 661 209 350 755.5 0 350 210 379 755.5 0 379 211 165 755.5 0 165 212 447 755.5 0 447 213 348 755.5 0 348 214 222 755.5 0 222 215 220 755.5 0 220 216 271 755.5 0 271 217 250 755.5 0 250 218 127 755.5 0 127 219 123 755.5 0 123 220 348 755.5 0 348 221 614 755.5 0 614 222 818 755.5 0 818 223 420 755.5 0 420 224 318 755.5 0 318 225 362 755.5 0 362 226 234 755.5 0 234 227 176 755.5 0 176 228 204 755.5 0 204 229 102 755.5 0 102 230 76 755.5 0 76 231 126 755.5 0 126 232 215 755.5 0 215 233 187 755.5 0 187 234 234 755.5 0 234 235 235 755.5 0 235 236 117 755.5 0 117 237 93 755.5 0 93 238 81 755.5 0 81 239 55 755.5 0 55 240 53 755.5 0 53 241 177 755.5 0 177 242 229 755.5 0 229 243 513 755.5 0 513 244 224 755.5 0 224 245 211 755.5 0 211 246 113 755.5 0 113 247 136 755.5 0 136 248 74 755.5 0 74 249 51 755.5 0 51 250 108 755.5 0 108 251 323 755.5 0 323 252 517 755.5 0 517 253 312 755.5 0 312 254 1534 755.5 0 1534 255 11958 755.5 0 11958 256 54563 755.5 0 54563 257 5328 755.5 0 5328 258 1560 755.5 0 1560 259 218 755.5 0 218 260 104 755.5 0 104 261 144 755.5 0 144 262 165 755.5 0 165 263 280 755.5 0 280 264 328 755.5 0 328 265 269 755.5 0 269 266 485 755.5 0 485 267 229 755.5 0 229 268 189 755.5 0 189 269 72 755.5 0 72 270 163 755.5 0 163 271 185 755.5 0 185 272 130 755.5 0 130 273 106 755.5 0 106 274 85 755.5 0 85 275 89 755.5 0 89 276 103 755.5 0 103 277 122 755.5 0 122 278 167 755.5 0 167 279 242 755.5 0 242 280 319 755.5 0 319 281 188 755.5 0 188 282 208 755.5 0 208 283 317 755.5 0 317 284 249 755.5 0 249 285 117 755.5 0 117 286 130 755.5 0 130 287 593 755.5 0 593 288 146 755.5 0 146 289 75 755.5 0 75 290 181 755.5 0 181 291 216 755.5 0 216 292 329 755.5 0 329 293 257 755.5 0 257 294 114 755.5 0 114 295 75 755.5 0 75 296 327 755.5 0 327 297 127 755.5 0 127 298 315 755.5 0 315 299 447 755.5 0 447 300 128 755.5 0 128 301 21 755.5 0 21 Finished in 2.13 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 48,482 Reads with adapters: 4,394 (9.1%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 4,384 (9.0%) Total basepairs processed: 9,130,852 bp Total written (filtered): 1,196,950 bp (13.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4394 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 0 1 19 46 0.0 3 0 43 1 1 1 20 4138 0.0 4 4047 81 6 3 1 21 18 0.0 4 7 9 0 1 1 22 3 0.0 4 0 3 23 139 0.0 4 130 4 1 4 28 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 1 1 100 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 127 35 0.0 4 0 0 0 0 35 144 1 0.0 4 0 0 0 0 1 168 2 0.0 4 0 0 0 0 2 175 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (36 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 4,384 Reads with adapters: 598 (13.6%) Reads written (passing filters): 4,384 (100.0%) Total basepairs processed: 1,196,950 bp Total written (filtered): 1,073,844 bp (89.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 598 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 1.0% G: 23.4% T: 2.0% none/other: 72.1% Overview of removed sequences length count expect max.err error counts 3 1 68.5 0 1 4 3 17.1 0 3 9 1 0.0 0 0 1 10 3 0.0 1 0 3 11 137 0.0 1 0 137 12 8 0.0 1 0 8 13 1 0.0 1 0 1 64 1 0.0 1 1 259 1 0.0 1 1 267 1 0.0 1 0 1 271 2 0.0 1 2 273 8 0.0 1 8 274 405 0.0 1 392 13 275 26 0.0 1 26