This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.94 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 28,853 (0.9%) Reads that were too short: 9,424 (0.3%) Reads written (passing filters): 19,429 (0.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 3,274,454 bp (0.4%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 28853 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 100 755.5 0 100 7 86 755.5 0 86 8 108 755.5 0 108 9 4552 755.5 0 4552 10 21 755.5 0 21 11 13 755.5 0 13 12 18 755.5 0 18 13 13 755.5 0 13 14 13 755.5 0 13 15 15 755.5 0 15 16 16 755.5 0 16 17 21 755.5 0 21 18 29 755.5 0 29 19 21 755.5 0 21 20 21 755.5 0 21 21 15 755.5 0 15 22 23 755.5 0 23 23 22 755.5 0 22 24 15 755.5 0 15 25 14 755.5 0 14 26 23 755.5 0 23 27 19 755.5 0 19 28 18 755.5 0 18 29 17 755.5 0 17 30 19 755.5 0 19 31 26 755.5 0 26 32 16 755.5 0 16 33 15 755.5 0 15 34 20 755.5 0 20 35 20 755.5 0 20 36 14 755.5 0 14 37 14 755.5 0 14 38 24 755.5 0 24 39 19 755.5 0 19 40 20 755.5 0 20 41 17 755.5 0 17 42 16 755.5 0 16 43 24 755.5 0 24 44 160 755.5 0 160 45 15 755.5 0 15 46 17 755.5 0 17 47 22 755.5 0 22 48 19 755.5 0 19 49 15 755.5 0 15 50 16 755.5 0 16 51 17 755.5 0 17 52 41 755.5 0 41 53 18 755.5 0 18 54 12 755.5 0 12 55 13 755.5 0 13 56 13 755.5 0 13 57 4 755.5 0 4 58 15 755.5 0 15 59 15 755.5 0 15 60 4 755.5 0 4 61 12 755.5 0 12 62 44 755.5 0 44 63 5 755.5 0 5 64 8 755.5 0 8 65 7 755.5 0 7 66 17 755.5 0 17 67 14 755.5 0 14 68 4 755.5 0 4 69 9 755.5 0 9 70 14 755.5 0 14 71 9 755.5 0 9 72 9 755.5 0 9 73 8 755.5 0 8 74 7 755.5 0 7 75 52 755.5 0 52 76 24 755.5 0 24 77 69 755.5 0 69 78 9 755.5 0 9 79 8 755.5 0 8 80 10 755.5 0 10 81 24 755.5 0 24 82 5 755.5 0 5 83 7 755.5 0 7 84 6 755.5 0 6 85 6 755.5 0 6 86 7 755.5 0 7 87 5 755.5 0 5 88 10 755.5 0 10 89 10 755.5 0 10 90 10 755.5 0 10 91 14 755.5 0 14 92 101 755.5 0 101 93 8 755.5 0 8 94 33 755.5 0 33 95 7 755.5 0 7 96 21 755.5 0 21 97 7 755.5 0 7 98 6 755.5 0 6 99 3 755.5 0 3 100 3 755.5 0 3 101 10 755.5 0 10 102 6 755.5 0 6 103 10 755.5 0 10 104 3 755.5 0 3 105 6 755.5 0 6 106 5 755.5 0 5 107 12 755.5 0 12 108 3 755.5 0 3 109 14 755.5 0 14 110 9 755.5 0 9 111 10 755.5 0 10 112 15 755.5 0 15 113 25 755.5 0 25 114 52 755.5 0 52 115 7 755.5 0 7 116 12 755.5 0 12 117 13 755.5 0 13 118 16 755.5 0 16 119 19 755.5 0 19 120 11 755.5 0 11 121 21 755.5 0 21 122 17 755.5 0 17 123 191 755.5 0 191 124 50 755.5 0 50 125 168 755.5 0 168 126 10 755.5 0 10 127 12 755.5 0 12 128 9 755.5 0 9 129 16 755.5 0 16 130 12 755.5 0 12 131 27 755.5 0 27 132 53 755.5 0 53 133 11 755.5 0 11 134 7 755.5 0 7 135 4 755.5 0 4 136 19 755.5 0 19 137 18 755.5 0 18 138 6 755.5 0 6 139 9 755.5 0 9 140 14 755.5 0 14 141 8 755.5 0 8 142 6 755.5 0 6 143 5 755.5 0 5 144 8 755.5 0 8 145 6 755.5 0 6 146 26 755.5 0 26 147 18 755.5 0 18 148 38 755.5 0 38 149 9 755.5 0 9 150 8 755.5 0 8 151 8 755.5 0 8 152 6 755.5 0 6 153 2 755.5 0 2 154 10 755.5 0 10 155 7 755.5 0 7 156 8 755.5 0 8 157 7 755.5 0 7 158 32 755.5 0 32 159 12 755.5 0 12 160 14 755.5 0 14 161 13 755.5 0 13 162 8 755.5 0 8 163 12 755.5 0 12 164 6 755.5 0 6 165 24 755.5 0 24 166 14 755.5 0 14 167 21 755.5 0 21 168 8 755.5 0 8 169 12 755.5 0 12 170 43 755.5 0 43 171 19 755.5 0 19 172 50 755.5 0 50 173 50 755.5 0 50 174 637 755.5 0 637 175 497 755.5 0 497 176 74 755.5 0 74 177 38 755.5 0 38 178 25 755.5 0 25 179 46 755.5 0 46 180 135 755.5 0 135 181 158 755.5 0 158 182 27 755.5 0 27 183 13 755.5 0 13 184 18 755.5 0 18 185 17 755.5 0 17 186 22 755.5 0 22 187 36 755.5 0 36 188 56 755.5 0 56 189 12 755.5 0 12 190 7 755.5 0 7 191 16 755.5 0 16 192 31 755.5 0 31 193 41 755.5 0 41 194 170 755.5 0 170 195 58 755.5 0 58 196 21 755.5 0 21 197 17 755.5 0 17 198 114 755.5 0 114 199 1841 755.5 0 1841 200 7150 755.5 0 7150 201 177 755.5 0 177 202 13 755.5 0 13 203 5 755.5 0 5 204 12 755.5 0 12 205 18 755.5 0 18 206 26 755.5 0 26 207 16 755.5 0 16 208 20 755.5 0 20 209 10 755.5 0 10 210 3 755.5 0 3 211 16 755.5 0 16 212 12 755.5 0 12 213 16 755.5 0 16 214 47 755.5 0 47 215 25 755.5 0 25 216 16 755.5 0 16 217 10 755.5 0 10 218 14 755.5 0 14 219 33 755.5 0 33 220 108 755.5 0 108 221 43 755.5 0 43 222 50 755.5 0 50 223 45 755.5 0 45 224 21 755.5 0 21 225 24 755.5 0 24 226 18 755.5 0 18 227 17 755.5 0 17 228 28 755.5 0 28 229 22 755.5 0 22 230 12 755.5 0 12 231 14 755.5 0 14 232 9 755.5 0 9 233 9 755.5 0 9 234 11 755.5 0 11 235 15 755.5 0 15 236 28 755.5 0 28 237 44 755.5 0 44 238 222 755.5 0 222 239 503 755.5 0 503 240 6625 755.5 0 6625 241 156 755.5 0 156 242 102 755.5 0 102 243 5 755.5 0 5 244 11 755.5 0 11 245 13 755.5 0 13 246 20 755.5 0 20 247 29 755.5 0 29 248 42 755.5 0 42 249 34 755.5 0 34 250 18 755.5 0 18 251 10 755.5 0 10 252 6 755.5 0 6 253 13 755.5 0 13 254 11 755.5 0 11 255 10 755.5 0 10 256 29 755.5 0 29 257 73 755.5 0 73 258 102 755.5 0 102 259 7 755.5 0 7 260 6 755.5 0 6 261 4 755.5 0 4 262 5 755.5 0 5 263 9 755.5 0 9 264 10 755.5 0 10 265 21 755.5 0 21 266 13 755.5 0 13 267 28 755.5 0 28 268 19 755.5 0 19 269 14 755.5 0 14 270 4 755.5 0 4 272 4 755.5 0 4 273 6 755.5 0 6 274 10 755.5 0 10 275 11 755.5 0 11 276 8 755.5 0 8 277 6 755.5 0 6 278 10 755.5 0 10 279 7 755.5 0 7 280 19 755.5 0 19 281 10 755.5 0 10 282 4 755.5 0 4 283 8 755.5 0 8 284 9 755.5 0 9 285 14 755.5 0 14 286 27 755.5 0 27 287 38 755.5 0 38 288 69 755.5 0 69 289 33 755.5 0 33 290 25 755.5 0 25 291 31 755.5 0 31 292 7 755.5 0 7 293 12 755.5 0 12 294 14 755.5 0 14 295 6 755.5 0 6 296 6 755.5 0 6 297 2 755.5 0 2 298 5 755.5 0 5 299 4 755.5 0 4 300 6 755.5 0 6 301 9 755.5 0 9 Finished in 0.73 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 19,429 Reads with adapters: 4,750 (24.4%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 4,745 (24.4%) Total basepairs processed: 3,274,454 bp Total written (filtered): 1,304,580 bp (39.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4750 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 37 0.0 3 0 35 1 1 17 4596 0.0 3 4506 85 5 18 21 0.0 3 0 9 11 1 19 18 0.0 3 0 1 2 15 20 67 0.0 3 63 0 0 4 24 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 4,745 Reads with adapters: 594 (12.5%) Reads written (passing filters): 4,745 (100.0%) Total basepairs processed: 1,304,580 bp Total written (filtered): 1,302,797 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 594 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.8% C: 2.7% G: 45.3% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 593 74.1 0 593 4 1 18.5 0 1