This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 91.50 s (30 us/read; 2.03 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 106,524 (3.4%) Reads that were too short: 3,398 (0.1%) Reads written (passing filters): 103,126 (3.3%) Total basepairs processed: 931,490,553 bp Total written (filtered): 18,349,333 bp (2.0%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 106524 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2427 755.5 0 2427 7 11803 755.5 0 11803 8 140 755.5 0 140 9 3054 755.5 0 3054 10 21 755.5 0 21 11 28 755.5 0 28 12 9 755.5 0 9 13 20 755.5 0 20 14 13 755.5 0 13 15 27 755.5 0 27 16 24 755.5 0 24 17 30 755.5 0 30 18 47 755.5 0 47 19 49 755.5 0 49 20 99 755.5 0 99 21 41 755.5 0 41 22 15 755.5 0 15 23 6 755.5 0 6 24 17 755.5 0 17 25 12 755.5 0 12 26 23 755.5 0 23 27 47 755.5 0 47 28 59 755.5 0 59 29 17 755.5 0 17 30 69 755.5 0 69 31 16 755.5 0 16 32 23 755.5 0 23 33 12 755.5 0 12 34 15 755.5 0 15 35 19 755.5 0 19 36 18 755.5 0 18 37 14 755.5 0 14 38 12 755.5 0 12 39 25 755.5 0 25 40 304 755.5 0 304 41 18 755.5 0 18 42 26 755.5 0 26 43 11 755.5 0 11 44 21 755.5 0 21 45 12 755.5 0 12 46 18 755.5 0 18 47 24 755.5 0 24 48 9 755.5 0 9 49 90 755.5 0 90 50 20 755.5 0 20 51 19 755.5 0 19 52 8 755.5 0 8 53 11 755.5 0 11 54 15 755.5 0 15 55 44 755.5 0 44 56 24 755.5 0 24 57 23 755.5 0 23 58 17 755.5 0 17 59 18 755.5 0 18 60 15 755.5 0 15 61 20 755.5 0 20 62 18 755.5 0 18 63 11 755.5 0 11 64 23 755.5 0 23 65 12 755.5 0 12 66 15 755.5 0 15 67 11 755.5 0 11 68 35 755.5 0 35 69 17 755.5 0 17 70 7 755.5 0 7 71 22 755.5 0 22 72 5 755.5 0 5 73 9 755.5 0 9 74 7 755.5 0 7 75 17 755.5 0 17 76 18 755.5 0 18 77 17 755.5 0 17 78 12 755.5 0 12 79 11 755.5 0 11 80 10 755.5 0 10 81 14 755.5 0 14 82 14 755.5 0 14 83 14 755.5 0 14 84 40 755.5 0 40 85 21 755.5 0 21 86 25 755.5 0 25 87 20 755.5 0 20 88 20 755.5 0 20 89 20 755.5 0 20 90 20 755.5 0 20 91 25 755.5 0 25 92 21 755.5 0 21 93 20 755.5 0 20 94 14 755.5 0 14 95 29 755.5 0 29 96 13 755.5 0 13 97 22 755.5 0 22 98 19 755.5 0 19 99 10 755.5 0 10 100 18 755.5 0 18 101 19 755.5 0 19 102 14 755.5 0 14 103 22 755.5 0 22 104 16 755.5 0 16 105 33 755.5 0 33 106 27 755.5 0 27 107 39 755.5 0 39 108 12 755.5 0 12 109 6 755.5 0 6 110 15 755.5 0 15 111 24 755.5 0 24 112 15 755.5 0 15 113 16 755.5 0 16 114 37 755.5 0 37 115 44 755.5 0 44 116 512 755.5 0 512 117 29 755.5 0 29 118 16 755.5 0 16 119 15 755.5 0 15 120 31 755.5 0 31 121 26 755.5 0 26 122 24 755.5 0 24 123 34 755.5 0 34 124 24 755.5 0 24 125 20 755.5 0 20 126 59 755.5 0 59 127 34 755.5 0 34 128 74 755.5 0 74 129 126 755.5 0 126 130 80 755.5 0 80 131 69 755.5 0 69 132 116 755.5 0 116 133 194 755.5 0 194 134 570 755.5 0 570 135 150 755.5 0 150 136 190 755.5 0 190 137 365 755.5 0 365 138 177 755.5 0 177 139 178 755.5 0 178 140 442 755.5 0 442 141 295 755.5 0 295 142 277 755.5 0 277 143 458 755.5 0 458 144 677 755.5 0 677 145 861 755.5 0 861 146 859 755.5 0 859 147 14469 755.5 0 14469 148 12329 755.5 0 12329 149 44860 755.5 0 44860 150 304 755.5 0 304 151 17 755.5 0 17 152 19 755.5 0 19 153 13 755.5 0 13 154 121 755.5 0 121 155 44 755.5 0 44 156 31 755.5 0 31 157 11 755.5 0 11 158 8 755.5 0 8 159 25 755.5 0 25 160 19 755.5 0 19 161 18 755.5 0 18 162 12 755.5 0 12 163 25 755.5 0 25 164 24 755.5 0 24 165 29 755.5 0 29 166 11 755.5 0 11 167 19 755.5 0 19 168 28 755.5 0 28 169 21 755.5 0 21 170 47 755.5 0 47 171 34 755.5 0 34 172 18 755.5 0 18 173 175 755.5 0 175 174 32 755.5 0 32 175 26 755.5 0 26 176 9 755.5 0 9 177 12 755.5 0 12 178 12 755.5 0 12 179 17 755.5 0 17 180 17 755.5 0 17 181 13 755.5 0 13 182 19 755.5 0 19 183 26 755.5 0 26 184 42 755.5 0 42 185 18 755.5 0 18 186 33 755.5 0 33 187 77 755.5 0 77 188 1567 755.5 0 1567 189 55 755.5 0 55 190 42 755.5 0 42 191 13 755.5 0 13 192 70 755.5 0 70 193 18 755.5 0 18 194 22 755.5 0 22 195 20 755.5 0 20 196 29 755.5 0 29 197 30 755.5 0 30 198 24 755.5 0 24 199 79 755.5 0 79 200 172 755.5 0 172 201 886 755.5 0 886 202 765 755.5 0 765 203 477 755.5 0 477 204 95 755.5 0 95 205 32 755.5 0 32 206 20 755.5 0 20 207 11 755.5 0 11 208 27 755.5 0 27 209 10 755.5 0 10 210 7 755.5 0 7 211 11 755.5 0 11 212 9 755.5 0 9 213 9 755.5 0 9 214 7 755.5 0 7 215 21 755.5 0 21 216 11 755.5 0 11 217 13 755.5 0 13 218 12 755.5 0 12 219 10 755.5 0 10 220 10 755.5 0 10 221 6 755.5 0 6 222 11 755.5 0 11 223 27 755.5 0 27 224 28 755.5 0 28 225 46 755.5 0 46 226 55 755.5 0 55 227 12 755.5 0 12 228 22 755.5 0 22 229 18 755.5 0 18 230 24 755.5 0 24 231 13 755.5 0 13 232 7 755.5 0 7 233 8 755.5 0 8 234 7 755.5 0 7 235 8 755.5 0 8 236 20 755.5 0 20 237 10 755.5 0 10 238 17 755.5 0 17 239 7 755.5 0 7 240 8 755.5 0 8 241 11 755.5 0 11 242 32 755.5 0 32 243 18 755.5 0 18 244 15 755.5 0 15 245 11 755.5 0 11 246 15 755.5 0 15 247 9 755.5 0 9 248 5 755.5 0 5 249 22 755.5 0 22 250 25 755.5 0 25 251 10 755.5 0 10 252 42 755.5 0 42 253 54 755.5 0 54 254 63 755.5 0 63 255 24 755.5 0 24 256 22 755.5 0 22 257 8 755.5 0 8 258 10 755.5 0 10 259 39 755.5 0 39 260 11 755.5 0 11 261 11 755.5 0 11 262 7 755.5 0 7 263 8 755.5 0 8 264 8 755.5 0 8 265 8 755.5 0 8 266 5 755.5 0 5 267 13 755.5 0 13 268 15 755.5 0 15 269 55 755.5 0 55 270 34 755.5 0 34 271 34 755.5 0 34 272 8 755.5 0 8 273 36 755.5 0 36 274 8 755.5 0 8 275 21 755.5 0 21 276 19 755.5 0 19 277 38 755.5 0 38 278 10 755.5 0 10 279 13 755.5 0 13 280 9 755.5 0 9 281 19 755.5 0 19 282 104 755.5 0 104 283 13 755.5 0 13 284 21 755.5 0 21 285 89 755.5 0 89 286 9 755.5 0 9 287 6 755.5 0 6 288 5 755.5 0 5 289 10 755.5 0 10 290 10 755.5 0 10 291 23 755.5 0 23 292 15 755.5 0 15 293 74 755.5 0 74 294 105 755.5 0 105 295 35 755.5 0 35 296 9 755.5 0 9 297 8 755.5 0 8 299 3 755.5 0 3 300 118 755.5 0 118 301 5 755.5 0 5 Finished in 4.43 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 103,126 Reads with adapters: 3,272 (3.2%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 3,255 (3.2%) Total basepairs processed: 18,349,333 bp Total written (filtered): 880,943 bp (4.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3272 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 19 59 0.0 3 0 58 0 0 1 20 3133 0.0 4 3036 81 13 2 1 21 12 0.0 4 4 8 22 2 0.0 4 1 1 23 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 1 48 1 0.0 4 1 55 1 0.0 4 1 78 2 0.0 4 0 0 0 0 2 79 2 0.0 4 0 0 0 0 2 89 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 127 41 0.0 4 0 0 0 0 41 128 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 3,255 Reads with adapters: 326 (10.0%) Reads written (passing filters): 3,255 (100.0%) Total basepairs processed: 880,943 bp Total written (filtered): 872,592 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 326 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.7% C: 2.1% G: 89.0% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 47 0.0 0 0 47 10 42 0.0 1 1 41 11 213 0.0 1 0 213 12 2 0.0 1 0 2 208 1 0.0 1 1 228 16 0.0 1 14 2 231 1 0.0 1 1 257 1 0.0 1 0 1 263 1 0.0 1 1 267 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.