This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.49 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 35,085 (1.1%) Reads that were too short: 21,512 (0.7%) Reads written (passing filters): 13,573 (0.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 2,801,147 bp (0.3%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 35085 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 97 755.5 0 97 7 3257 755.5 0 3257 8 10 755.5 0 10 9 73 755.5 0 73 10 21 755.5 0 21 11 16 755.5 0 16 12 17 755.5 0 17 13 14 755.5 0 14 14 10 755.5 0 10 15 26 755.5 0 26 16 8 755.5 0 8 17 5 755.5 0 5 18 18 755.5 0 18 19 13 755.5 0 13 20 13 755.5 0 13 21 6 755.5 0 6 22 10 755.5 0 10 23 10 755.5 0 10 24 9 755.5 0 9 25 12 755.5 0 12 26 15 755.5 0 15 27 14 755.5 0 14 28 15 755.5 0 15 29 18 755.5 0 18 30 24 755.5 0 24 31 12 755.5 0 12 32 18 755.5 0 18 33 13 755.5 0 13 34 17 755.5 0 17 35 8 755.5 0 8 36 12 755.5 0 12 37 16 755.5 0 16 38 21 755.5 0 21 39 14 755.5 0 14 40 27 755.5 0 27 41 21 755.5 0 21 42 15 755.5 0 15 43 14 755.5 0 14 44 17 755.5 0 17 45 11 755.5 0 11 46 18 755.5 0 18 47 10 755.5 0 10 48 4 755.5 0 4 49 29 755.5 0 29 50 11 755.5 0 11 51 25 755.5 0 25 52 36 755.5 0 36 53 9 755.5 0 9 54 21 755.5 0 21 55 47 755.5 0 47 56 73 755.5 0 73 57 38 755.5 0 38 58 33 755.5 0 33 59 20 755.5 0 20 60 14 755.5 0 14 61 14 755.5 0 14 62 42 755.5 0 42 63 18 755.5 0 18 64 12 755.5 0 12 65 8 755.5 0 8 66 7 755.5 0 7 67 19 755.5 0 19 68 9 755.5 0 9 69 9 755.5 0 9 70 22 755.5 0 22 71 18 755.5 0 18 72 20 755.5 0 20 73 17 755.5 0 17 74 9 755.5 0 9 75 15 755.5 0 15 76 7 755.5 0 7 77 13 755.5 0 13 78 12 755.5 0 12 79 58 755.5 0 58 80 40 755.5 0 40 81 48 755.5 0 48 82 33 755.5 0 33 83 230 755.5 0 230 84 12 755.5 0 12 85 17 755.5 0 17 86 16 755.5 0 16 87 11 755.5 0 11 88 5 755.5 0 5 89 12 755.5 0 12 90 10 755.5 0 10 91 12 755.5 0 12 92 12 755.5 0 12 93 11 755.5 0 11 94 11 755.5 0 11 95 18 755.5 0 18 96 10 755.5 0 10 97 18 755.5 0 18 98 39 755.5 0 39 99 67 755.5 0 67 100 40 755.5 0 40 101 31 755.5 0 31 102 13 755.5 0 13 103 17 755.5 0 17 104 10 755.5 0 10 105 28 755.5 0 28 106 24 755.5 0 24 107 33 755.5 0 33 108 30 755.5 0 30 109 26 755.5 0 26 110 30 755.5 0 30 111 41 755.5 0 41 112 38 755.5 0 38 113 91 755.5 0 91 114 1111 755.5 0 1111 115 2534 755.5 0 2534 116 26 755.5 0 26 117 14 755.5 0 14 118 7 755.5 0 7 119 15 755.5 0 15 120 13 755.5 0 13 121 10 755.5 0 10 122 15 755.5 0 15 123 16 755.5 0 16 124 18 755.5 0 18 125 17 755.5 0 17 126 21 755.5 0 21 127 7 755.5 0 7 128 16 755.5 0 16 129 32 755.5 0 32 130 43 755.5 0 43 131 17 755.5 0 17 132 27 755.5 0 27 133 23 755.5 0 23 134 18 755.5 0 18 135 21 755.5 0 21 136 13 755.5 0 13 137 16 755.5 0 16 138 10 755.5 0 10 139 21 755.5 0 21 140 26 755.5 0 26 141 21 755.5 0 21 142 24 755.5 0 24 143 47 755.5 0 47 144 65 755.5 0 65 145 31 755.5 0 31 146 17 755.5 0 17 147 12 755.5 0 12 148 11 755.5 0 11 149 9 755.5 0 9 150 71 755.5 0 71 151 8 755.5 0 8 152 13 755.5 0 13 153 12 755.5 0 12 154 28 755.5 0 28 155 98 755.5 0 98 156 200 755.5 0 200 157 108 755.5 0 108 158 94 755.5 0 94 159 10 755.5 0 10 160 22 755.5 0 22 161 6 755.5 0 6 162 18 755.5 0 18 163 21 755.5 0 21 164 22 755.5 0 22 165 38 755.5 0 38 166 65 755.5 0 65 167 857 755.5 0 857 168 82 755.5 0 82 169 1048 755.5 0 1048 170 14 755.5 0 14 171 13 755.5 0 13 172 8 755.5 0 8 173 14 755.5 0 14 174 9 755.5 0 9 175 5 755.5 0 5 176 10 755.5 0 10 177 11 755.5 0 11 178 10 755.5 0 10 179 18 755.5 0 18 180 15 755.5 0 15 181 10 755.5 0 10 182 10 755.5 0 10 183 16 755.5 0 16 184 39 755.5 0 39 185 18 755.5 0 18 186 15 755.5 0 15 187 15 755.5 0 15 188 154 755.5 0 154 189 17 755.5 0 17 190 13 755.5 0 13 191 16 755.5 0 16 192 10 755.5 0 10 193 18 755.5 0 18 194 8 755.5 0 8 195 8 755.5 0 8 196 9 755.5 0 9 197 6 755.5 0 6 198 16 755.5 0 16 199 14 755.5 0 14 200 78 755.5 0 78 201 23 755.5 0 23 202 35 755.5 0 35 203 22 755.5 0 22 204 29 755.5 0 29 205 14 755.5 0 14 206 10 755.5 0 10 207 20 755.5 0 20 208 15 755.5 0 15 209 7 755.5 0 7 210 15 755.5 0 15 211 21 755.5 0 21 212 34 755.5 0 34 213 80 755.5 0 80 214 20 755.5 0 20 215 10 755.5 0 10 216 19 755.5 0 19 217 74 755.5 0 74 218 21 755.5 0 21 219 10 755.5 0 10 220 4 755.5 0 4 221 17 755.5 0 17 222 10 755.5 0 10 223 12 755.5 0 12 224 21 755.5 0 21 225 17 755.5 0 17 226 20 755.5 0 20 227 18 755.5 0 18 228 9 755.5 0 9 229 16 755.5 0 16 230 42 755.5 0 42 231 16 755.5 0 16 232 13 755.5 0 13 233 25 755.5 0 25 234 10 755.5 0 10 235 10 755.5 0 10 236 18 755.5 0 18 237 18 755.5 0 18 238 11 755.5 0 11 239 3 755.5 0 3 240 10 755.5 0 10 241 14 755.5 0 14 242 8 755.5 0 8 243 25 755.5 0 25 244 3 755.5 0 3 245 23 755.5 0 23 246 10 755.5 0 10 247 9 755.5 0 9 248 9 755.5 0 9 249 4 755.5 0 4 250 16 755.5 0 16 251 13 755.5 0 13 252 20 755.5 0 20 253 23 755.5 0 23 254 13 755.5 0 13 255 18 755.5 0 18 256 69 755.5 0 69 257 28 755.5 0 28 258 15 755.5 0 15 259 29 755.5 0 29 260 12 755.5 0 12 261 12 755.5 0 12 262 22 755.5 0 22 263 23 755.5 0 23 264 21 755.5 0 21 265 11 755.5 0 11 266 6 755.5 0 6 267 9 755.5 0 9 268 13 755.5 0 13 269 14 755.5 0 14 270 19 755.5 0 19 271 21 755.5 0 21 272 27 755.5 0 27 273 16 755.5 0 16 274 13 755.5 0 13 275 9 755.5 0 9 276 27 755.5 0 27 277 13 755.5 0 13 278 29 755.5 0 29 279 44 755.5 0 44 280 28 755.5 0 28 281 26 755.5 0 26 282 13 755.5 0 13 283 30 755.5 0 30 284 65 755.5 0 65 285 83 755.5 0 83 286 35 755.5 0 35 287 27 755.5 0 27 288 69 755.5 0 69 289 25 755.5 0 25 290 41 755.5 0 41 291 123 755.5 0 123 292 264 755.5 0 264 293 244 755.5 0 244 294 203 755.5 0 203 295 359 755.5 0 359 296 1020 755.5 0 1020 297 6214 755.5 0 6214 298 5746 755.5 0 5746 299 2040 755.5 0 2040 300 3331 755.5 0 3331 301 40 755.5 0 40 Finished in 0.62 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 13,573 Reads with adapters: 3,284 (24.2%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 3,282 (24.2%) Total basepairs processed: 2,801,147 bp Total written (filtered): 908,619 bp (32.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3284 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 25 0.0 3 0 24 1 17 3219 0.0 3 3134 85 18 12 0.0 3 0 8 4 19 17 0.0 3 5 0 6 6 20 4 0.0 3 0 0 0 4 52 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 1 85 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,282 Reads with adapters: 252 (7.7%) Reads written (passing filters): 3,282 (100.0%) Total basepairs processed: 908,619 bp Total written (filtered): 907,860 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 252 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.3% C: 77.4% G: 9.5% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 249 51.3 0 249 4 3 12.8 0 3