This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.00 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 621,017 (20.1%) Reads that were too short: 257,562 (8.3%) Reads written (passing filters): 363,455 (11.7%) Total basepairs processed: 931,490,553 bp Total written (filtered): 61,377,826 bp (6.6%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 621017 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3295 755.5 0 3295 7 3018 755.5 0 3018 8 129 755.5 0 129 9 6116 755.5 0 6116 10 23 755.5 0 23 11 11 755.5 0 11 12 1 755.5 0 1 13 7 755.5 0 7 14 8 755.5 0 8 15 11 755.5 0 11 16 6 755.5 0 6 17 6 755.5 0 6 18 1 755.5 0 1 19 6 755.5 0 6 20 4 755.5 0 4 21 7 755.5 0 7 22 12 755.5 0 12 23 6 755.5 0 6 24 4 755.5 0 4 25 11 755.5 0 11 26 6 755.5 0 6 27 11 755.5 0 11 28 18 755.5 0 18 29 20 755.5 0 20 30 35 755.5 0 35 31 18 755.5 0 18 32 49 755.5 0 49 33 21 755.5 0 21 34 13 755.5 0 13 35 15 755.5 0 15 36 12 755.5 0 12 37 8 755.5 0 8 38 5 755.5 0 5 39 14 755.5 0 14 40 11 755.5 0 11 41 52 755.5 0 52 42 51 755.5 0 51 43 281 755.5 0 281 44 17 755.5 0 17 45 4 755.5 0 4 46 4 755.5 0 4 47 4 755.5 0 4 48 4 755.5 0 4 49 15 755.5 0 15 50 5 755.5 0 5 51 9 755.5 0 9 52 11 755.5 0 11 53 5 755.5 0 5 54 5 755.5 0 5 55 3 755.5 0 3 56 9 755.5 0 9 57 18 755.5 0 18 58 9 755.5 0 9 59 43 755.5 0 43 60 9 755.5 0 9 61 6 755.5 0 6 62 46 755.5 0 46 63 19 755.5 0 19 64 23 755.5 0 23 65 25 755.5 0 25 66 56 755.5 0 56 67 21 755.5 0 21 68 58 755.5 0 58 69 296 755.5 0 296 70 68 755.5 0 68 71 356 755.5 0 356 72 1539 755.5 0 1539 73 5988 755.5 0 5988 74 1831 755.5 0 1831 75 2696 755.5 0 2696 76 1420 755.5 0 1420 77 1587 755.5 0 1587 78 2628 755.5 0 2628 79 1914 755.5 0 1914 80 2635 755.5 0 2635 81 1601 755.5 0 1601 82 1543 755.5 0 1543 83 1051 755.5 0 1051 84 1065 755.5 0 1065 85 1618 755.5 0 1618 86 1927 755.5 0 1927 87 819 755.5 0 819 88 1117 755.5 0 1117 89 3394 755.5 0 3394 90 1757 755.5 0 1757 91 5528 755.5 0 5528 92 1635 755.5 0 1635 93 2173 755.5 0 2173 94 2090 755.5 0 2090 95 5182 755.5 0 5182 96 2274 755.5 0 2274 97 1422 755.5 0 1422 98 735 755.5 0 735 99 1235 755.5 0 1235 100 1210 755.5 0 1210 101 2804 755.5 0 2804 102 2828 755.5 0 2828 103 3454 755.5 0 3454 104 2298 755.5 0 2298 105 7859 755.5 0 7859 106 7105 755.5 0 7105 107 4947 755.5 0 4947 108 2388 755.5 0 2388 109 1957 755.5 0 1957 110 1612 755.5 0 1612 111 3518 755.5 0 3518 112 2932 755.5 0 2932 113 3628 755.5 0 3628 114 3455 755.5 0 3455 115 3405 755.5 0 3405 116 1414 755.5 0 1414 117 1943 755.5 0 1943 118 2090 755.5 0 2090 119 2910 755.5 0 2910 120 2754 755.5 0 2754 121 4272 755.5 0 4272 122 2193 755.5 0 2193 123 3647 755.5 0 3647 124 3191 755.5 0 3191 125 3318 755.5 0 3318 126 4118 755.5 0 4118 127 3588 755.5 0 3588 128 2716 755.5 0 2716 129 1829 755.5 0 1829 130 2483 755.5 0 2483 131 1661 755.5 0 1661 132 2989 755.5 0 2989 133 5232 755.5 0 5232 134 3861 755.5 0 3861 135 3226 755.5 0 3226 136 2451 755.5 0 2451 137 2078 755.5 0 2078 138 1828 755.5 0 1828 139 1905 755.5 0 1905 140 2488 755.5 0 2488 141 2891 755.5 0 2891 142 1939 755.5 0 1939 143 2355 755.5 0 2355 144 1988 755.5 0 1988 145 3167 755.5 0 3167 146 3261 755.5 0 3261 147 2553 755.5 0 2553 148 3765 755.5 0 3765 149 3015 755.5 0 3015 150 3687 755.5 0 3687 151 2020 755.5 0 2020 152 2872 755.5 0 2872 153 3678 755.5 0 3678 154 3321 755.5 0 3321 155 2794 755.5 0 2794 156 2259 755.5 0 2259 157 2045 755.5 0 2045 158 2459 755.5 0 2459 159 2313 755.5 0 2313 160 2415 755.5 0 2415 161 3066 755.5 0 3066 162 4069 755.5 0 4069 163 3561 755.5 0 3561 164 3583 755.5 0 3583 165 2845 755.5 0 2845 166 2426 755.5 0 2426 167 1957 755.5 0 1957 168 1922 755.5 0 1922 169 2516 755.5 0 2516 170 2558 755.5 0 2558 171 1940 755.5 0 1940 172 3125 755.5 0 3125 173 2701 755.5 0 2701 174 5327 755.5 0 5327 175 2717 755.5 0 2717 176 2286 755.5 0 2286 177 1356 755.5 0 1356 178 2367 755.5 0 2367 179 2324 755.5 0 2324 180 2873 755.5 0 2873 181 2511 755.5 0 2511 182 1893 755.5 0 1893 183 2120 755.5 0 2120 184 2292 755.5 0 2292 185 2777 755.5 0 2777 186 3425 755.5 0 3425 187 2783 755.5 0 2783 188 2602 755.5 0 2602 189 2065 755.5 0 2065 190 2681 755.5 0 2681 191 3154 755.5 0 3154 192 3230 755.5 0 3230 193 3071 755.5 0 3071 194 2667 755.5 0 2667 195 1258 755.5 0 1258 196 1503 755.5 0 1503 197 1524 755.5 0 1524 198 2866 755.5 0 2866 199 4407 755.5 0 4407 200 2326 755.5 0 2326 201 1525 755.5 0 1525 202 1659 755.5 0 1659 203 1683 755.5 0 1683 204 1808 755.5 0 1808 205 2045 755.5 0 2045 206 2404 755.5 0 2404 207 1845 755.5 0 1845 208 1926 755.5 0 1926 209 2348 755.5 0 2348 210 1742 755.5 0 1742 211 3012 755.5 0 3012 212 2246 755.5 0 2246 213 14943 755.5 0 14943 214 6281 755.5 0 6281 215 5530 755.5 0 5530 216 1973 755.5 0 1973 217 1986 755.5 0 1986 218 2624 755.5 0 2624 219 2907 755.5 0 2907 220 2493 755.5 0 2493 221 1688 755.5 0 1688 222 1813 755.5 0 1813 223 1854 755.5 0 1854 224 2568 755.5 0 2568 225 3145 755.5 0 3145 226 2412 755.5 0 2412 227 3411 755.5 0 3411 228 2352 755.5 0 2352 229 2670 755.5 0 2670 230 2881 755.5 0 2881 231 2419 755.5 0 2419 232 1525 755.5 0 1525 233 1540 755.5 0 1540 234 2398 755.5 0 2398 235 2772 755.5 0 2772 236 3114 755.5 0 3114 237 2771 755.5 0 2771 238 2511 755.5 0 2511 239 2777 755.5 0 2777 240 2126 755.5 0 2126 241 1512 755.5 0 1512 242 1604 755.5 0 1604 243 1534 755.5 0 1534 244 1735 755.5 0 1735 245 2000 755.5 0 2000 246 2487 755.5 0 2487 247 3475 755.5 0 3475 248 3389 755.5 0 3389 249 3161 755.5 0 3161 250 3551 755.5 0 3551 251 1823 755.5 0 1823 252 1546 755.5 0 1546 253 1952 755.5 0 1952 254 2117 755.5 0 2117 255 2869 755.5 0 2869 256 2985 755.5 0 2985 257 3441 755.5 0 3441 258 2438 755.5 0 2438 259 2602 755.5 0 2602 260 1983 755.5 0 1983 261 2106 755.5 0 2106 262 1799 755.5 0 1799 263 2734 755.5 0 2734 264 2492 755.5 0 2492 265 2575 755.5 0 2575 266 2322 755.5 0 2322 267 2375 755.5 0 2375 268 2161 755.5 0 2161 269 2167 755.5 0 2167 270 2012 755.5 0 2012 271 2188 755.5 0 2188 272 2766 755.5 0 2766 273 3431 755.5 0 3431 274 2732 755.5 0 2732 275 2363 755.5 0 2363 276 2266 755.5 0 2266 277 1736 755.5 0 1736 278 1815 755.5 0 1815 279 2558 755.5 0 2558 280 2896 755.5 0 2896 281 2154 755.5 0 2154 282 2841 755.5 0 2841 283 1906 755.5 0 1906 284 3975 755.5 0 3975 285 2897 755.5 0 2897 286 2785 755.5 0 2785 287 2289 755.5 0 2289 288 1606 755.5 0 1606 289 1744 755.5 0 1744 290 2067 755.5 0 2067 291 6264 755.5 0 6264 292 2509 755.5 0 2509 293 2292 755.5 0 2292 294 2255 755.5 0 2255 295 2189 755.5 0 2189 296 1782 755.5 0 1782 297 1322 755.5 0 1322 298 1118 755.5 0 1118 299 858 755.5 0 858 300 1797 755.5 0 1797 301 5012 755.5 0 5012 Finished in 15.57 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 363,455 Reads with adapters: 6,364 (1.8%) Reads that were too short: 113 (0.0%) Reads written (passing filters): 6,251 (1.7%) Total basepairs processed: 61,377,826 bp Total written (filtered): 1,701,256 bp (2.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6364 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 0 1 19 49 0.0 3 0 47 1 1 20 2938 0.0 4 2896 38 2 2 21 67 0.0 4 1 61 4 0 1 22 3119 0.0 4 3045 66 5 1 2 23 14 0.0 4 2 7 0 2 3 25 4 0.0 4 0 0 0 4 28 2 0.0 4 0 0 0 0 2 29 3 0.0 4 0 0 0 0 3 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 34 4 0.0 4 0 0 0 0 4 35 2 0.0 4 0 0 0 0 2 36 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 0 2 40 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 45 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 47 2 0.0 4 0 0 0 0 2 50 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 0 2 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 56 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 58 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 63 3 0.0 4 0 0 0 0 3 65 1 0.0 4 0 0 0 0 1 67 2 0.0 4 1 0 0 0 1 68 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 76 2 0.0 4 0 0 0 0 2 78 3 0.0 4 0 0 0 0 3 79 1 0.0 4 0 0 0 0 1 80 2 0.0 4 0 0 0 0 2 81 2 0.0 4 0 0 0 1 1 82 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 0 2 86 2 0.0 4 0 0 0 0 2 87 2 0.0 4 0 0 0 1 1 89 1 0.0 4 0 0 0 0 1 91 4 0.0 4 0 0 0 1 3 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 3 0.0 4 0 0 0 0 3 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 110 3 0.0 4 0 0 0 0 3 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 2 0.0 4 0 0 0 0 2 114 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 127 4 0.0 4 0 0 0 0 4 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 1 1 131 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 139 2 0.0 4 0 0 0 0 2 141 2 0.0 4 0 0 0 0 2 142 2 0.0 4 0 0 0 0 2 143 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 158 2 0.0 4 0 0 0 0 2 159 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 163 3 0.0 4 0 0 0 1 2 167 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 173 2 0.0 4 0 0 0 0 2 174 1 0.0 4 0 0 0 0 1 175 2 0.0 4 0 0 0 0 2 176 1 0.0 4 0 0 0 0 1 178 2 0.0 4 0 0 0 0 2 181 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 188 2 0.0 4 0 0 0 0 2 191 2 0.0 4 0 0 0 0 2 197 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.26 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 6,251 Reads with adapters: 2,110 (33.8%) Reads written (passing filters): 6,251 (100.0%) Total basepairs processed: 1,701,256 bp Total written (filtered): 1,185,067 bp (69.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2110 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 93.5% C: 0.2% G: 6.1% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 97.7 0 1 10 12 0.0 1 0 12 11 123 0.0 1 1 122 12 2 0.0 1 0 2 234 4 0.0 1 4 260 7 0.0 1 7 261 1881 0.0 1 1743 138 262 74 0.0 1 74 263 1 0.0 1 0 1 264 1 0.0 1 1 265 1 0.0 1 1 269 2 0.0 1 2 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.