This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 97.95 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 42,413 (1.4%) Reads that were too short: 15,339 (0.5%) Reads written (passing filters): 27,074 (0.9%) Total basepairs processed: 931,490,553 bp Total written (filtered): 5,631,841 bp (0.6%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 42413 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 24 755.5 0 24 7 66 755.5 0 66 8 66 755.5 0 66 9 2913 755.5 0 2913 10 22 755.5 0 22 11 21 755.5 0 21 12 30 755.5 0 30 13 26 755.5 0 26 14 20 755.5 0 20 15 19 755.5 0 19 16 20 755.5 0 20 17 33 755.5 0 33 18 29 755.5 0 29 19 26 755.5 0 26 20 32 755.5 0 32 21 27 755.5 0 27 22 46 755.5 0 46 23 18 755.5 0 18 24 31 755.5 0 31 25 46 755.5 0 46 26 33 755.5 0 33 27 42 755.5 0 42 28 29 755.5 0 29 29 28 755.5 0 28 30 30 755.5 0 30 31 37 755.5 0 37 32 37 755.5 0 37 33 38 755.5 0 38 34 51 755.5 0 51 35 258 755.5 0 258 36 382 755.5 0 382 37 2340 755.5 0 2340 38 24 755.5 0 24 39 21 755.5 0 21 40 16 755.5 0 16 41 16 755.5 0 16 42 26 755.5 0 26 43 18 755.5 0 18 44 25 755.5 0 25 45 26 755.5 0 26 46 15 755.5 0 15 47 20 755.5 0 20 48 14 755.5 0 14 49 16 755.5 0 16 50 18 755.5 0 18 51 16 755.5 0 16 52 23 755.5 0 23 53 26 755.5 0 26 54 14 755.5 0 14 55 12 755.5 0 12 56 22 755.5 0 22 57 11 755.5 0 11 58 15 755.5 0 15 59 22 755.5 0 22 60 26 755.5 0 26 61 20 755.5 0 20 62 15 755.5 0 15 63 24 755.5 0 24 64 28 755.5 0 28 65 20 755.5 0 20 66 21 755.5 0 21 67 27 755.5 0 27 68 33 755.5 0 33 69 334 755.5 0 334 70 19 755.5 0 19 71 28 755.5 0 28 72 80 755.5 0 80 73 18 755.5 0 18 74 16 755.5 0 16 75 18 755.5 0 18 76 23 755.5 0 23 77 14 755.5 0 14 78 22 755.5 0 22 79 22 755.5 0 22 80 45 755.5 0 45 81 40 755.5 0 40 82 57 755.5 0 57 83 32 755.5 0 32 84 33 755.5 0 33 85 23 755.5 0 23 86 16 755.5 0 16 87 30 755.5 0 30 88 107 755.5 0 107 89 196 755.5 0 196 90 1574 755.5 0 1574 91 22 755.5 0 22 92 111 755.5 0 111 93 53 755.5 0 53 94 59 755.5 0 59 95 45 755.5 0 45 96 74 755.5 0 74 97 253 755.5 0 253 98 694 755.5 0 694 99 4680 755.5 0 4680 100 2095 755.5 0 2095 101 217 755.5 0 217 102 74 755.5 0 74 103 59 755.5 0 59 104 180 755.5 0 180 105 75 755.5 0 75 106 80 755.5 0 80 107 70 755.5 0 70 108 49 755.5 0 49 109 102 755.5 0 102 110 200 755.5 0 200 111 226 755.5 0 226 112 519 755.5 0 519 113 73 755.5 0 73 114 52 755.5 0 52 115 47 755.5 0 47 116 52 755.5 0 52 117 22 755.5 0 22 118 59 755.5 0 59 119 37 755.5 0 37 120 77 755.5 0 77 121 169 755.5 0 169 122 139 755.5 0 139 123 76 755.5 0 76 124 86 755.5 0 86 125 40 755.5 0 40 126 55 755.5 0 55 127 75 755.5 0 75 128 60 755.5 0 60 129 87 755.5 0 87 130 81 755.5 0 81 131 111 755.5 0 111 132 112 755.5 0 112 133 138 755.5 0 138 134 90 755.5 0 90 135 60 755.5 0 60 136 55 755.5 0 55 137 62 755.5 0 62 138 66 755.5 0 66 139 143 755.5 0 143 140 69 755.5 0 69 141 101 755.5 0 101 142 142 755.5 0 142 143 61 755.5 0 61 144 72 755.5 0 72 145 56 755.5 0 56 146 40 755.5 0 40 147 66 755.5 0 66 148 63 755.5 0 63 149 89 755.5 0 89 150 35 755.5 0 35 151 29 755.5 0 29 152 45 755.5 0 45 153 44 755.5 0 44 154 49 755.5 0 49 155 101 755.5 0 101 156 59 755.5 0 59 157 70 755.5 0 70 158 53 755.5 0 53 159 225 755.5 0 225 160 60 755.5 0 60 161 226 755.5 0 226 162 50 755.5 0 50 163 40 755.5 0 40 164 81 755.5 0 81 165 66 755.5 0 66 166 77 755.5 0 77 167 109 755.5 0 109 168 228 755.5 0 228 169 53 755.5 0 53 170 60 755.5 0 60 171 58 755.5 0 58 172 60 755.5 0 60 173 68 755.5 0 68 174 43 755.5 0 43 175 55 755.5 0 55 176 42 755.5 0 42 177 52 755.5 0 52 178 44 755.5 0 44 179 34 755.5 0 34 180 39 755.5 0 39 181 57 755.5 0 57 182 67 755.5 0 67 183 95 755.5 0 95 184 94 755.5 0 94 185 79 755.5 0 79 186 40 755.5 0 40 187 52 755.5 0 52 188 57 755.5 0 57 189 75 755.5 0 75 190 62 755.5 0 62 191 86 755.5 0 86 192 77 755.5 0 77 193 90 755.5 0 90 194 64 755.5 0 64 195 174 755.5 0 174 196 92 755.5 0 92 197 174 755.5 0 174 198 56 755.5 0 56 199 46 755.5 0 46 200 115 755.5 0 115 201 150 755.5 0 150 202 156 755.5 0 156 203 152 755.5 0 152 204 65 755.5 0 65 205 196 755.5 0 196 206 428 755.5 0 428 207 497 755.5 0 497 208 267 755.5 0 267 209 199 755.5 0 199 210 85 755.5 0 85 211 116 755.5 0 116 212 109 755.5 0 109 213 101 755.5 0 101 214 78 755.5 0 78 215 98 755.5 0 98 216 71 755.5 0 71 217 77 755.5 0 77 218 58 755.5 0 58 219 55 755.5 0 55 220 99 755.5 0 99 221 165 755.5 0 165 222 96 755.5 0 96 223 162 755.5 0 162 224 93 755.5 0 93 225 98 755.5 0 98 226 71 755.5 0 71 227 85 755.5 0 85 228 88 755.5 0 88 229 87 755.5 0 87 230 89 755.5 0 89 231 86 755.5 0 86 232 114 755.5 0 114 233 105 755.5 0 105 234 113 755.5 0 113 235 272 755.5 0 272 236 1049 755.5 0 1049 237 780 755.5 0 780 238 95 755.5 0 95 239 126 755.5 0 126 240 166 755.5 0 166 241 996 755.5 0 996 242 361 755.5 0 361 243 163 755.5 0 163 244 73 755.5 0 73 245 46 755.5 0 46 246 46 755.5 0 46 247 36 755.5 0 36 248 85 755.5 0 85 249 124 755.5 0 124 250 311 755.5 0 311 251 79 755.5 0 79 252 46 755.5 0 46 253 33 755.5 0 33 254 77 755.5 0 77 255 78 755.5 0 78 256 49 755.5 0 49 257 64 755.5 0 64 258 79 755.5 0 79 259 154 755.5 0 154 260 549 755.5 0 549 261 880 755.5 0 880 262 458 755.5 0 458 263 113 755.5 0 113 264 78 755.5 0 78 265 101 755.5 0 101 266 46 755.5 0 46 267 32 755.5 0 32 268 57 755.5 0 57 269 86 755.5 0 86 270 115 755.5 0 115 271 79 755.5 0 79 272 106 755.5 0 106 273 123 755.5 0 123 274 123 755.5 0 123 275 180 755.5 0 180 276 71 755.5 0 71 277 70 755.5 0 70 278 152 755.5 0 152 279 65 755.5 0 65 280 138 755.5 0 138 281 118 755.5 0 118 282 172 755.5 0 172 283 138 755.5 0 138 284 71 755.5 0 71 285 59 755.5 0 59 286 44 755.5 0 44 287 49 755.5 0 49 288 71 755.5 0 71 289 145 755.5 0 145 290 136 755.5 0 136 291 84 755.5 0 84 292 54 755.5 0 54 293 39 755.5 0 39 294 64 755.5 0 64 295 70 755.5 0 70 296 85 755.5 0 85 297 121 755.5 0 121 298 128 755.5 0 128 299 103 755.5 0 103 300 119 755.5 0 119 301 100 755.5 0 100 Finished in 1.22 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 27,074 Reads with adapters: 2,999 (11.1%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 2,990 (11.0%) Total basepairs processed: 5,631,841 bp Total written (filtered): 816,783 bp (14.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2999 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 0 2 16 16 0.0 3 0 14 1 1 17 2914 0.0 3 2860 52 0 2 18 11 0.0 3 3 6 2 19 4 0.0 3 0 0 1 3 20 8 0.0 3 0 0 0 8 31 1 0.0 3 1 35 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 5 0.0 3 0 0 0 5 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 1 106 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 4 0.0 3 0 0 0 4 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 2,990 Reads with adapters: 226 (7.6%) Reads written (passing filters): 2,990 (100.0%) Total basepairs processed: 816,783 bp Total written (filtered): 816,098 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 226 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.7% C: 15.0% G: 11.9% T: 9.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 219 46.7 0 219 4 7 11.7 0 7