This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.51 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 151,599 (4.9%) Reads that were too short: 7,754 (0.3%) Reads written (passing filters): 143,845 (4.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 24,044,746 bp (2.6%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 151599 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 60 755.5 0 60 7 21 755.5 0 21 8 373 755.5 0 373 9 25 755.5 0 25 10 9 755.5 0 9 11 11 755.5 0 11 12 9 755.5 0 9 13 11 755.5 0 11 14 12 755.5 0 12 15 9 755.5 0 9 16 9 755.5 0 9 17 20 755.5 0 20 18 10 755.5 0 10 19 11 755.5 0 11 20 9 755.5 0 9 21 9 755.5 0 9 22 16 755.5 0 16 23 14 755.5 0 14 24 11 755.5 0 11 25 8 755.5 0 8 26 11 755.5 0 11 27 10 755.5 0 10 28 8 755.5 0 8 29 8 755.5 0 8 30 7 755.5 0 7 31 17 755.5 0 17 32 18 755.5 0 18 33 12 755.5 0 12 34 13 755.5 0 13 35 21 755.5 0 21 36 43 755.5 0 43 37 15 755.5 0 15 38 23 755.5 0 23 39 13 755.5 0 13 40 17 755.5 0 17 41 19 755.5 0 19 42 17 755.5 0 17 43 11 755.5 0 11 44 11 755.5 0 11 45 7 755.5 0 7 46 22 755.5 0 22 47 10 755.5 0 10 48 8 755.5 0 8 49 22 755.5 0 22 50 16 755.5 0 16 51 12 755.5 0 12 52 13 755.5 0 13 53 25 755.5 0 25 54 43 755.5 0 43 55 22 755.5 0 22 56 14 755.5 0 14 57 15 755.5 0 15 58 17 755.5 0 17 59 28 755.5 0 28 60 20 755.5 0 20 61 28 755.5 0 28 62 17 755.5 0 17 63 21 755.5 0 21 64 46 755.5 0 46 65 24 755.5 0 24 66 16 755.5 0 16 67 15 755.5 0 15 68 18 755.5 0 18 69 20 755.5 0 20 70 14 755.5 0 14 71 15 755.5 0 15 72 16 755.5 0 16 73 18 755.5 0 18 74 20 755.5 0 20 75 22 755.5 0 22 76 51 755.5 0 51 77 46 755.5 0 46 78 71 755.5 0 71 79 143 755.5 0 143 80 358 755.5 0 358 81 5271 755.5 0 5271 82 1809 755.5 0 1809 83 1326 755.5 0 1326 84 18 755.5 0 18 85 28 755.5 0 28 86 26 755.5 0 26 87 40 755.5 0 40 88 16 755.5 0 16 89 20 755.5 0 20 90 8 755.5 0 8 91 16 755.5 0 16 92 24 755.5 0 24 93 33 755.5 0 33 94 16 755.5 0 16 95 16 755.5 0 16 96 21 755.5 0 21 97 29 755.5 0 29 98 51 755.5 0 51 99 68 755.5 0 68 100 39 755.5 0 39 101 19 755.5 0 19 102 38 755.5 0 38 103 40 755.5 0 40 104 70 755.5 0 70 105 67 755.5 0 67 106 98 755.5 0 98 107 113 755.5 0 113 108 209 755.5 0 209 109 514 755.5 0 514 110 103 755.5 0 103 111 157 755.5 0 157 112 384 755.5 0 384 113 208 755.5 0 208 114 199 755.5 0 199 115 311 755.5 0 311 116 465 755.5 0 465 117 361 755.5 0 361 118 517 755.5 0 517 119 538 755.5 0 538 120 716 755.5 0 716 121 728 755.5 0 728 122 6935 755.5 0 6935 123 13431 755.5 0 13431 124 69463 755.5 0 69463 125 318 755.5 0 318 126 54 755.5 0 54 127 23 755.5 0 23 128 29 755.5 0 29 129 32 755.5 0 32 130 43 755.5 0 43 131 32 755.5 0 32 132 21 755.5 0 21 133 31 755.5 0 31 134 34 755.5 0 34 135 39 755.5 0 39 136 32 755.5 0 32 137 40 755.5 0 40 138 35 755.5 0 35 139 50 755.5 0 50 140 19 755.5 0 19 141 18 755.5 0 18 142 26 755.5 0 26 143 61 755.5 0 61 144 99 755.5 0 99 145 224 755.5 0 224 146 991 755.5 0 991 147 44 755.5 0 44 148 29 755.5 0 29 149 20 755.5 0 20 150 12 755.5 0 12 151 58 755.5 0 58 152 55 755.5 0 55 153 81 755.5 0 81 154 29 755.5 0 29 155 127 755.5 0 127 156 25 755.5 0 25 157 26 755.5 0 26 158 37 755.5 0 37 159 41 755.5 0 41 160 14 755.5 0 14 161 32 755.5 0 32 162 50 755.5 0 50 163 40 755.5 0 40 164 111 755.5 0 111 165 306 755.5 0 306 166 64 755.5 0 64 167 94 755.5 0 94 168 57 755.5 0 57 169 49 755.5 0 49 170 271 755.5 0 271 171 53 755.5 0 53 172 123 755.5 0 123 173 100 755.5 0 100 174 45 755.5 0 45 175 82 755.5 0 82 176 81 755.5 0 81 177 151 755.5 0 151 178 169 755.5 0 169 179 208 755.5 0 208 180 507 755.5 0 507 181 1458 755.5 0 1458 182 17949 755.5 0 17949 183 7557 755.5 0 7557 184 3853 755.5 0 3853 185 75 755.5 0 75 186 27 755.5 0 27 187 11 755.5 0 11 188 28 755.5 0 28 189 99 755.5 0 99 190 185 755.5 0 185 191 37 755.5 0 37 192 14 755.5 0 14 193 20 755.5 0 20 194 22 755.5 0 22 195 42 755.5 0 42 196 55 755.5 0 55 197 87 755.5 0 87 198 31 755.5 0 31 199 19 755.5 0 19 200 17 755.5 0 17 201 34 755.5 0 34 202 34 755.5 0 34 203 19 755.5 0 19 204 19 755.5 0 19 205 24 755.5 0 24 206 33 755.5 0 33 207 24 755.5 0 24 208 24 755.5 0 24 209 32 755.5 0 32 210 37 755.5 0 37 211 45 755.5 0 45 212 29 755.5 0 29 213 18 755.5 0 18 214 33 755.5 0 33 215 44 755.5 0 44 216 38 755.5 0 38 217 51 755.5 0 51 218 23 755.5 0 23 219 23 755.5 0 23 220 46 755.5 0 46 221 63 755.5 0 63 222 77 755.5 0 77 223 44 755.5 0 44 224 19 755.5 0 19 225 44 755.5 0 44 226 37 755.5 0 37 227 34 755.5 0 34 228 30 755.5 0 30 229 17 755.5 0 17 230 13 755.5 0 13 231 19 755.5 0 19 232 24 755.5 0 24 233 25 755.5 0 25 234 54 755.5 0 54 235 14 755.5 0 14 236 21 755.5 0 21 237 21 755.5 0 21 238 33 755.5 0 33 239 21 755.5 0 21 240 215 755.5 0 215 241 67 755.5 0 67 242 94 755.5 0 94 243 42 755.5 0 42 244 35 755.5 0 35 245 42 755.5 0 42 246 27 755.5 0 27 247 26 755.5 0 26 248 8 755.5 0 8 249 45 755.5 0 45 250 26 755.5 0 26 251 53 755.5 0 53 252 37 755.5 0 37 253 28 755.5 0 28 254 40 755.5 0 40 255 39 755.5 0 39 256 20 755.5 0 20 257 17 755.5 0 17 258 20 755.5 0 20 259 26 755.5 0 26 260 61 755.5 0 61 261 307 755.5 0 307 262 264 755.5 0 264 263 439 755.5 0 439 264 46 755.5 0 46 265 54 755.5 0 54 266 36 755.5 0 36 267 22 755.5 0 22 268 46 755.5 0 46 269 53 755.5 0 53 270 40 755.5 0 40 271 38 755.5 0 38 272 42 755.5 0 42 273 34 755.5 0 34 274 26 755.5 0 26 275 53 755.5 0 53 276 64 755.5 0 64 277 80 755.5 0 80 278 52 755.5 0 52 279 17 755.5 0 17 280 29 755.5 0 29 281 32 755.5 0 32 282 63 755.5 0 63 283 109 755.5 0 109 284 52 755.5 0 52 285 64 755.5 0 64 286 25 755.5 0 25 287 70 755.5 0 70 288 26 755.5 0 26 289 58 755.5 0 58 290 655 755.5 0 655 291 1612 755.5 0 1612 292 261 755.5 0 261 293 69 755.5 0 69 294 50 755.5 0 50 295 81 755.5 0 81 296 216 755.5 0 216 297 153 755.5 0 153 298 87 755.5 0 87 299 77 755.5 0 77 300 55 755.5 0 55 301 23 755.5 0 23 Finished in 5.57 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 143,845 Reads with adapters: 909 (0.6%) Reads that were too short: 380 (0.3%) Reads written (passing filters): 529 (0.4%) Total basepairs processed: 24,044,746 bp Total written (filtered): 121,132 bp (0.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 909 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 2 0.0 3 0 2 17 337 0.0 3 329 6 2 18 10 0.0 3 0 1 7 2 19 10 0.0 3 0 0 3 7 20 1 0.0 3 0 0 0 1 22 2 0.0 3 0 0 0 2 24 2 0.0 3 0 0 0 2 25 2 0.0 3 0 0 1 1 34 13 0.0 3 0 0 0 13 47 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 3 0.0 3 0 0 0 3 50 106 0.0 3 0 0 0 106 51 11 0.0 3 0 0 0 11 52 4 0.0 3 0 0 0 4 53 19 0.0 3 0 0 0 19 54 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 161 8 0.0 3 0 0 0 8 162 43 0.0 3 0 0 0 43 163 9 0.0 3 0 0 0 9 164 37 0.0 3 0 0 0 37 165 266 0.0 3 0 0 1 265 166 12 0.0 3 0 0 0 12 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 529 Reads with adapters: 98 (18.5%) Reads written (passing filters): 529 (100.0%) Total basepairs processed: 121,132 bp Total written (filtered): 120,836 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 98 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.6% C: 9.2% G: 9.2% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 96 8.3 0 96 4 2 2.1 0 2