This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 101.86 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 626,511 (20.2%) Reads that were too short: 6,622 (0.2%) Reads written (passing filters): 619,889 (20.0%) Total basepairs processed: 931,490,553 bp Total written (filtered): 180,198,459 bp (19.3%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 626511 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3547 755.5 0 3547 7 4136 755.5 0 4136 8 9743 755.5 0 9743 9 593649 755.5 0 593649 10 593 755.5 0 593 11 20 755.5 0 20 12 16 755.5 0 16 13 11 755.5 0 11 14 8 755.5 0 8 15 10 755.5 0 10 16 30 755.5 0 30 17 244 755.5 0 244 18 145 755.5 0 145 19 14 755.5 0 14 20 10 755.5 0 10 21 17 755.5 0 17 22 80 755.5 0 80 23 97 755.5 0 97 24 4 755.5 0 4 25 13 755.5 0 13 26 74 755.5 0 74 27 26 755.5 0 26 28 117 755.5 0 117 29 54 755.5 0 54 30 250 755.5 0 250 31 164 755.5 0 164 32 7 755.5 0 7 33 8 755.5 0 8 34 14 755.5 0 14 35 17 755.5 0 17 36 4 755.5 0 4 37 4 755.5 0 4 38 9 755.5 0 9 39 7 755.5 0 7 40 6 755.5 0 6 41 4 755.5 0 4 42 11 755.5 0 11 43 5 755.5 0 5 44 8 755.5 0 8 45 17 755.5 0 17 46 7 755.5 0 7 47 6 755.5 0 6 48 5 755.5 0 5 49 8 755.5 0 8 50 7 755.5 0 7 51 22 755.5 0 22 52 13 755.5 0 13 53 6 755.5 0 6 54 9 755.5 0 9 55 6 755.5 0 6 56 8 755.5 0 8 57 9 755.5 0 9 58 4 755.5 0 4 59 7 755.5 0 7 60 6 755.5 0 6 61 4 755.5 0 4 62 7 755.5 0 7 63 8 755.5 0 8 64 17 755.5 0 17 65 10 755.5 0 10 66 15 755.5 0 15 67 18 755.5 0 18 68 26 755.5 0 26 69 498 755.5 0 498 70 22 755.5 0 22 71 155 755.5 0 155 72 17 755.5 0 17 73 11 755.5 0 11 74 6 755.5 0 6 75 21 755.5 0 21 76 23 755.5 0 23 77 290 755.5 0 290 78 41 755.5 0 41 79 92 755.5 0 92 80 13 755.5 0 13 81 51 755.5 0 51 82 48 755.5 0 48 83 21 755.5 0 21 84 34 755.5 0 34 85 49 755.5 0 49 86 26 755.5 0 26 87 21 755.5 0 21 88 41 755.5 0 41 89 11 755.5 0 11 90 19 755.5 0 19 91 35 755.5 0 35 92 30 755.5 0 30 93 23 755.5 0 23 94 33 755.5 0 33 95 5 755.5 0 5 96 7 755.5 0 7 97 34 755.5 0 34 98 38 755.5 0 38 99 40 755.5 0 40 100 6 755.5 0 6 101 12 755.5 0 12 102 7 755.5 0 7 103 2 755.5 0 2 104 7 755.5 0 7 105 10 755.5 0 10 106 5 755.5 0 5 107 10 755.5 0 10 108 8 755.5 0 8 109 13 755.5 0 13 110 4 755.5 0 4 111 18 755.5 0 18 112 21 755.5 0 21 113 22 755.5 0 22 114 54 755.5 0 54 115 52 755.5 0 52 116 64 755.5 0 64 117 14 755.5 0 14 118 16 755.5 0 16 119 16 755.5 0 16 120 15 755.5 0 15 121 18 755.5 0 18 122 20 755.5 0 20 123 27 755.5 0 27 124 15 755.5 0 15 125 25 755.5 0 25 126 11 755.5 0 11 127 18 755.5 0 18 128 14 755.5 0 14 129 31 755.5 0 31 130 27 755.5 0 27 131 19 755.5 0 19 132 35 755.5 0 35 133 253 755.5 0 253 134 42 755.5 0 42 135 44 755.5 0 44 136 39 755.5 0 39 137 84 755.5 0 84 138 71 755.5 0 71 139 162 755.5 0 162 140 69 755.5 0 69 141 21 755.5 0 21 142 26 755.5 0 26 143 43 755.5 0 43 144 28 755.5 0 28 145 45 755.5 0 45 146 37 755.5 0 37 147 41 755.5 0 41 148 181 755.5 0 181 149 403 755.5 0 403 150 287 755.5 0 287 151 374 755.5 0 374 152 135 755.5 0 135 153 50 755.5 0 50 154 19 755.5 0 19 155 34 755.5 0 34 156 31 755.5 0 31 157 23 755.5 0 23 158 18 755.5 0 18 159 43 755.5 0 43 160 26 755.5 0 26 161 31 755.5 0 31 162 35 755.5 0 35 163 32 755.5 0 32 164 37 755.5 0 37 165 32 755.5 0 32 166 30 755.5 0 30 167 32 755.5 0 32 168 52 755.5 0 52 169 21 755.5 0 21 170 20 755.5 0 20 171 21 755.5 0 21 172 44 755.5 0 44 173 13 755.5 0 13 174 17 755.5 0 17 175 20 755.5 0 20 176 24 755.5 0 24 177 19 755.5 0 19 178 21 755.5 0 21 179 25 755.5 0 25 180 24 755.5 0 24 181 30 755.5 0 30 182 46 755.5 0 46 183 62 755.5 0 62 184 98 755.5 0 98 185 57 755.5 0 57 186 66 755.5 0 66 187 12 755.5 0 12 188 20 755.5 0 20 189 59 755.5 0 59 190 68 755.5 0 68 191 78 755.5 0 78 192 53 755.5 0 53 193 17 755.5 0 17 194 16 755.5 0 16 195 18 755.5 0 18 196 75 755.5 0 75 197 33 755.5 0 33 198 7 755.5 0 7 199 7 755.5 0 7 200 26 755.5 0 26 201 50 755.5 0 50 202 43 755.5 0 43 203 27 755.5 0 27 204 36 755.5 0 36 205 40 755.5 0 40 206 26 755.5 0 26 207 24 755.5 0 24 208 48 755.5 0 48 209 77 755.5 0 77 210 51 755.5 0 51 211 85 755.5 0 85 212 45 755.5 0 45 213 59 755.5 0 59 214 62 755.5 0 62 215 74 755.5 0 74 216 61 755.5 0 61 217 48 755.5 0 48 218 42 755.5 0 42 219 25 755.5 0 25 220 29 755.5 0 29 221 49 755.5 0 49 222 71 755.5 0 71 223 26 755.5 0 26 224 28 755.5 0 28 225 46 755.5 0 46 226 59 755.5 0 59 227 42 755.5 0 42 228 37 755.5 0 37 229 34 755.5 0 34 230 26 755.5 0 26 231 30 755.5 0 30 232 36 755.5 0 36 233 69 755.5 0 69 234 55 755.5 0 55 235 43 755.5 0 43 236 50 755.5 0 50 237 49 755.5 0 49 238 49 755.5 0 49 239 63 755.5 0 63 240 78 755.5 0 78 241 53 755.5 0 53 242 39 755.5 0 39 243 43 755.5 0 43 244 41 755.5 0 41 245 31 755.5 0 31 246 45 755.5 0 45 247 67 755.5 0 67 248 58 755.5 0 58 249 138 755.5 0 138 250 200 755.5 0 200 251 121 755.5 0 121 252 50 755.5 0 50 253 60 755.5 0 60 254 82 755.5 0 82 255 102 755.5 0 102 256 28 755.5 0 28 257 19 755.5 0 19 258 39 755.5 0 39 259 71 755.5 0 71 260 28 755.5 0 28 261 29 755.5 0 29 262 27 755.5 0 27 263 35 755.5 0 35 264 31 755.5 0 31 265 42 755.5 0 42 266 39 755.5 0 39 267 20 755.5 0 20 268 37 755.5 0 37 269 287 755.5 0 287 270 196 755.5 0 196 271 50 755.5 0 50 272 35 755.5 0 35 273 62 755.5 0 62 274 72 755.5 0 72 275 130 755.5 0 130 276 49 755.5 0 49 277 48 755.5 0 48 278 44 755.5 0 44 279 30 755.5 0 30 280 36 755.5 0 36 281 40 755.5 0 40 282 66 755.5 0 66 283 67 755.5 0 67 284 58 755.5 0 58 285 37 755.5 0 37 286 42 755.5 0 42 287 46 755.5 0 46 288 98 755.5 0 98 289 251 755.5 0 251 290 81 755.5 0 81 291 108 755.5 0 108 292 153 755.5 0 153 293 62 755.5 0 62 294 69 755.5 0 69 295 162 755.5 0 162 296 114 755.5 0 114 297 53 755.5 0 53 298 144 755.5 0 144 299 213 755.5 0 213 300 193 755.5 0 193 301 109 755.5 0 109 Finished in 39.39 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 619,889 Reads with adapters: 2,786 (0.4%) Reads that were too short: 107 (0.0%) Reads written (passing filters): 2,679 (0.4%) Total basepairs processed: 180,198,459 bp Total written (filtered): 440,153 bp (0.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2786 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 1 0.0 3 0 1 20 29 0.0 4 28 0 0 1 21 2 0.0 4 2 22 6 0.0 4 6 28 3 0.0 4 0 0 0 0 3 78 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 125 7 0.0 4 0 0 0 0 7 126 29 0.0 4 0 0 0 0 29 127 2330 0.0 4 1 0 0 0 2329 128 10 0.0 4 0 0 0 0 10 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 169 0.0 4 0 0 0 1 168 152 3 0.0 4 0 0 0 0 3 153 74 0.0 4 0 0 0 1 73 169 1 0.0 4 0 0 0 1 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 1 186 1 0.0 4 0 0 0 0 1 188 3 0.0 4 0 0 0 0 3 191 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 5 0.0 4 0 0 0 0 5 209 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 217 2 0.0 4 0 0 0 0 2 218 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 4 0.0 4 0 0 0 0 4 232 2 0.0 4 0 0 0 0 2 233 8 0.0 4 0 0 0 0 8 234 5 0.0 4 0 0 0 1 4 235 2 0.0 4 0 0 0 0 2 236 2 0.0 4 0 0 0 0 2 254 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 256 5 0.0 4 0 0 0 0 5 257 2 0.0 4 0 0 0 0 2 258 10 0.0 4 0 0 0 0 10 260 7 0.0 4 0 0 0 0 7 261 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 4 0.0 4 0 0 0 0 4 264 6 0.0 4 0 0 0 0 6 274 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 283 4 0.0 4 0 0 0 0 4 284 4 0.0 4 0 0 0 1 3 286 1 0.0 4 0 0 0 0 1 292 4 0.0 4 0 0 0 0 4 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (30 us/read; 2.01 M reads/minute). === Summary === Total reads processed: 2,679 Reads with adapters: 3 (0.1%) Reads written (passing filters): 2,679 (100.0%) Total basepairs processed: 440,153 bp Total written (filtered): 439,869 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 66.7% C: 0.0% G: 33.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 11 1 0.0 1 0 1 12 1 0.0 1 0 1 261 1 0.0 1 1