This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 97.71 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 536,850 (17.3%) Reads that were too short: 333,732 (10.8%) Reads written (passing filters): 203,118 (6.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 24,204,154 bp (2.6%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 536850 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 48 755.5 0 48 7 36 755.5 0 36 8 17 755.5 0 17 9 109 755.5 0 109 10 64 755.5 0 64 11 22 755.5 0 22 12 17 755.5 0 17 13 8 755.5 0 8 14 13 755.5 0 13 15 14 755.5 0 14 16 22 755.5 0 22 17 27 755.5 0 27 18 24 755.5 0 24 19 28 755.5 0 28 20 25 755.5 0 25 21 30 755.5 0 30 22 21 755.5 0 21 23 19 755.5 0 19 24 19 755.5 0 19 25 23 755.5 0 23 26 15 755.5 0 15 27 15 755.5 0 15 28 22 755.5 0 22 29 16 755.5 0 16 30 16 755.5 0 16 31 29 755.5 0 29 32 115 755.5 0 115 33 19 755.5 0 19 34 20 755.5 0 20 35 32 755.5 0 32 36 19 755.5 0 19 37 24 755.5 0 24 38 29 755.5 0 29 39 24 755.5 0 24 40 18 755.5 0 18 41 21 755.5 0 21 42 23 755.5 0 23 43 20 755.5 0 20 44 20 755.5 0 20 45 44 755.5 0 44 46 25 755.5 0 25 47 32 755.5 0 32 48 22 755.5 0 22 49 17 755.5 0 17 50 18 755.5 0 18 51 25 755.5 0 25 52 22 755.5 0 22 53 24 755.5 0 24 54 29 755.5 0 29 55 31 755.5 0 31 56 32 755.5 0 32 57 52 755.5 0 52 58 29 755.5 0 29 59 36 755.5 0 36 60 30 755.5 0 30 61 23 755.5 0 23 62 20 755.5 0 20 63 19 755.5 0 19 64 25 755.5 0 25 65 26 755.5 0 26 66 18 755.5 0 18 67 18 755.5 0 18 68 22 755.5 0 22 69 11 755.5 0 11 70 32 755.5 0 32 71 11 755.5 0 11 72 19 755.5 0 19 73 31 755.5 0 31 74 18 755.5 0 18 75 79 755.5 0 79 76 53 755.5 0 53 77 36 755.5 0 36 78 17 755.5 0 17 79 18 755.5 0 18 80 10 755.5 0 10 81 16 755.5 0 16 82 16 755.5 0 16 83 13 755.5 0 13 84 22 755.5 0 22 85 11 755.5 0 11 86 12 755.5 0 12 87 19 755.5 0 19 88 11 755.5 0 11 89 18 755.5 0 18 90 13 755.5 0 13 91 31 755.5 0 31 92 18 755.5 0 18 93 8 755.5 0 8 94 20 755.5 0 20 95 14 755.5 0 14 96 19 755.5 0 19 97 12 755.5 0 12 98 19 755.5 0 19 99 16 755.5 0 16 100 29 755.5 0 29 101 22 755.5 0 22 102 22 755.5 0 22 103 31 755.5 0 31 104 14 755.5 0 14 105 27 755.5 0 27 106 23 755.5 0 23 107 24 755.5 0 24 108 19 755.5 0 19 109 27 755.5 0 27 110 22 755.5 0 22 111 25 755.5 0 25 112 37 755.5 0 37 113 336 755.5 0 336 114 853 755.5 0 853 115 616 755.5 0 616 116 22 755.5 0 22 117 20 755.5 0 20 118 11 755.5 0 11 119 14 755.5 0 14 120 19 755.5 0 19 121 27 755.5 0 27 122 24 755.5 0 24 123 29 755.5 0 29 124 46 755.5 0 46 125 25 755.5 0 25 126 21 755.5 0 21 127 69 755.5 0 69 128 72 755.5 0 72 129 40 755.5 0 40 130 113 755.5 0 113 131 55 755.5 0 55 132 72 755.5 0 72 133 136 755.5 0 136 134 402 755.5 0 402 135 85 755.5 0 85 136 120 755.5 0 120 137 274 755.5 0 274 138 141 755.5 0 141 139 177 755.5 0 177 140 295 755.5 0 295 141 236 755.5 0 236 142 198 755.5 0 198 143 235 755.5 0 235 144 346 755.5 0 346 145 570 755.5 0 570 146 664 755.5 0 664 147 15996 755.5 0 15996 148 7249 755.5 0 7249 149 29578 755.5 0 29578 150 145 755.5 0 145 151 14 755.5 0 14 152 11 755.5 0 11 153 16 755.5 0 16 154 7 755.5 0 7 155 13 755.5 0 13 156 20 755.5 0 20 157 24 755.5 0 24 158 20 755.5 0 20 159 12 755.5 0 12 160 11 755.5 0 11 161 35 755.5 0 35 162 28 755.5 0 28 163 15 755.5 0 15 164 33 755.5 0 33 165 34 755.5 0 34 166 30 755.5 0 30 167 15 755.5 0 15 168 22 755.5 0 22 169 44 755.5 0 44 170 295 755.5 0 295 171 25 755.5 0 25 172 39 755.5 0 39 173 31 755.5 0 31 174 28 755.5 0 28 175 80 755.5 0 80 176 85 755.5 0 85 177 44 755.5 0 44 178 34 755.5 0 34 179 48 755.5 0 48 180 60 755.5 0 60 181 85 755.5 0 85 182 184 755.5 0 184 183 139 755.5 0 139 184 200 755.5 0 200 185 313 755.5 0 313 186 858 755.5 0 858 187 782 755.5 0 782 188 360 755.5 0 360 189 1371 755.5 0 1371 190 963 755.5 0 963 191 685 755.5 0 685 192 1126 755.5 0 1126 193 1425 755.5 0 1425 194 872 755.5 0 872 195 1047 755.5 0 1047 196 1576 755.5 0 1576 197 1942 755.5 0 1942 198 2349 755.5 0 2349 199 30256 755.5 0 30256 200 34422 755.5 0 34422 201 58972 755.5 0 58972 202 63191 755.5 0 63191 203 1419 755.5 0 1419 204 1465 755.5 0 1465 205 6820 755.5 0 6820 206 18081 755.5 0 18081 207 40058 755.5 0 40058 208 70551 755.5 0 70551 209 3597 755.5 0 3597 210 578 755.5 0 578 211 1211 755.5 0 1211 212 326 755.5 0 326 213 240 755.5 0 240 214 247 755.5 0 247 215 1620 755.5 0 1620 216 221 755.5 0 221 217 426 755.5 0 426 218 446 755.5 0 446 219 1502 755.5 0 1502 220 1006 755.5 0 1006 221 1073 755.5 0 1073 222 840 755.5 0 840 223 457 755.5 0 457 224 457 755.5 0 457 225 2763 755.5 0 2763 226 6654 755.5 0 6654 227 9604 755.5 0 9604 228 13764 755.5 0 13764 229 316 755.5 0 316 230 101 755.5 0 101 231 12 755.5 0 12 232 9 755.5 0 9 233 5 755.5 0 5 234 2 755.5 0 2 235 8 755.5 0 8 236 12 755.5 0 12 237 14 755.5 0 14 238 35 755.5 0 35 239 20 755.5 0 20 240 20 755.5 0 20 241 10 755.5 0 10 242 15 755.5 0 15 243 13 755.5 0 13 244 32 755.5 0 32 245 58 755.5 0 58 246 77 755.5 0 77 247 93 755.5 0 93 248 108 755.5 0 108 249 290 755.5 0 290 250 55 755.5 0 55 251 88 755.5 0 88 252 264 755.5 0 264 253 182 755.5 0 182 254 248 755.5 0 248 255 1523 755.5 0 1523 256 463 755.5 0 463 257 286 755.5 0 286 258 433 755.5 0 433 259 480 755.5 0 480 260 476 755.5 0 476 261 543 755.5 0 543 262 2242 755.5 0 2242 263 6658 755.5 0 6658 264 69259 755.5 0 69259 265 345 755.5 0 345 266 34 755.5 0 34 267 9 755.5 0 9 268 7 755.5 0 7 269 4 755.5 0 4 270 10 755.5 0 10 271 9 755.5 0 9 272 4 755.5 0 4 273 1 755.5 0 1 274 13 755.5 0 13 275 73 755.5 0 73 276 12 755.5 0 12 277 5 755.5 0 5 278 2 755.5 0 2 279 2 755.5 0 2 281 4 755.5 0 4 282 3 755.5 0 3 283 5 755.5 0 5 284 14 755.5 0 14 285 3 755.5 0 3 286 6 755.5 0 6 287 3 755.5 0 3 288 6 755.5 0 6 289 4 755.5 0 4 290 7 755.5 0 7 291 2 755.5 0 2 292 3 755.5 0 3 293 12 755.5 0 12 294 35 755.5 0 35 295 3 755.5 0 3 296 2 755.5 0 2 297 3 755.5 0 3 298 5 755.5 0 5 299 4 755.5 0 4 300 4 755.5 0 4 301 7 755.5 0 7 Finished in 6.44 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 203,118 Reads with adapters: 114 (0.1%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 97 (0.0%) Total basepairs processed: 24,204,154 bp Total written (filtered): 24,692 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 114 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 1 0.0 3 0 0 1 16 4 0.0 3 0 3 0 1 17 17 0.0 3 10 1 3 3 18 24 0.0 3 0 3 16 5 19 33 0.0 3 0 0 8 25 20 1 0.0 3 0 0 0 1 22 1 0.0 3 1 26 4 0.0 3 0 0 0 4 33 2 0.0 3 1 0 0 1 44 3 0.0 3 0 0 0 3 63 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 78 5 0.0 3 0 0 0 5 81 6 0.0 3 0 0 0 6 162 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 197 1 0.0 3 0 1 208 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (103 us/read; 0.58 M reads/minute). === Summary === Total reads processed: 97 Reads with adapters: 7 (7.2%) Reads written (passing filters): 97 (100.0%) Total basepairs processed: 24,692 bp Total written (filtered): 24,671 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 7 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.9% C: 28.6% G: 14.3% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 7 1.5 0 7