This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 99.67 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 89,679 (2.9%) Reads that were too short: 21,573 (0.7%) Reads written (passing filters): 68,106 (2.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 17,574,031 bp (1.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 89679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 470 755.5 0 470 7 647 755.5 0 647 8 14710 755.5 0 14710 9 34120 755.5 0 34120 10 93 755.5 0 93 11 3 755.5 0 3 12 4 755.5 0 4 13 4 755.5 0 4 15 2 755.5 0 2 16 2 755.5 0 2 17 3 755.5 0 3 18 4 755.5 0 4 19 3 755.5 0 3 20 5 755.5 0 5 21 3 755.5 0 3 22 2 755.5 0 2 23 2 755.5 0 2 24 3 755.5 0 3 25 1 755.5 0 1 26 2 755.5 0 2 27 9 755.5 0 9 28 21 755.5 0 21 29 5 755.5 0 5 30 1 755.5 0 1 31 1 755.5 0 1 32 6 755.5 0 6 33 5 755.5 0 5 34 13 755.5 0 13 35 4 755.5 0 4 36 4 755.5 0 4 37 3 755.5 0 3 38 6 755.5 0 6 39 12 755.5 0 12 40 6 755.5 0 6 41 6 755.5 0 6 42 10 755.5 0 10 43 8 755.5 0 8 44 5 755.5 0 5 45 8 755.5 0 8 46 1 755.5 0 1 47 2 755.5 0 2 48 10 755.5 0 10 49 15 755.5 0 15 50 9 755.5 0 9 51 6 755.5 0 6 52 11 755.5 0 11 53 4 755.5 0 4 54 14 755.5 0 14 55 16 755.5 0 16 56 4 755.5 0 4 57 13 755.5 0 13 58 34 755.5 0 34 59 91 755.5 0 91 60 1148 755.5 0 1148 61 113 755.5 0 113 62 298 755.5 0 298 63 15 755.5 0 15 64 126 755.5 0 126 65 42 755.5 0 42 66 718 755.5 0 718 67 309 755.5 0 309 68 49 755.5 0 49 69 2 755.5 0 2 70 4 755.5 0 4 71 9 755.5 0 9 72 8 755.5 0 8 73 8 755.5 0 8 74 7 755.5 0 7 75 7 755.5 0 7 76 15 755.5 0 15 77 71 755.5 0 71 78 15 755.5 0 15 79 99 755.5 0 99 80 51 755.5 0 51 81 109 755.5 0 109 82 196 755.5 0 196 83 69 755.5 0 69 84 64 755.5 0 64 85 50 755.5 0 50 86 64 755.5 0 64 87 23 755.5 0 23 88 19 755.5 0 19 89 52 755.5 0 52 90 23 755.5 0 23 91 82 755.5 0 82 92 127 755.5 0 127 93 39 755.5 0 39 94 65 755.5 0 65 95 59 755.5 0 59 96 28 755.5 0 28 97 184 755.5 0 184 98 35 755.5 0 35 99 41 755.5 0 41 100 8 755.5 0 8 101 16 755.5 0 16 102 20 755.5 0 20 103 39 755.5 0 39 104 18 755.5 0 18 105 14 755.5 0 14 106 14 755.5 0 14 107 12 755.5 0 12 108 48 755.5 0 48 109 43 755.5 0 43 110 13 755.5 0 13 111 37 755.5 0 37 112 83 755.5 0 83 113 127 755.5 0 127 114 125 755.5 0 125 115 754 755.5 0 754 116 606 755.5 0 606 117 160 755.5 0 160 118 36 755.5 0 36 119 12 755.5 0 12 120 47 755.5 0 47 121 60 755.5 0 60 122 59 755.5 0 59 123 87 755.5 0 87 124 66 755.5 0 66 125 65 755.5 0 65 126 45 755.5 0 45 127 78 755.5 0 78 128 57 755.5 0 57 129 55 755.5 0 55 130 68 755.5 0 68 131 41 755.5 0 41 132 47 755.5 0 47 133 60 755.5 0 60 134 62 755.5 0 62 135 52 755.5 0 52 136 75 755.5 0 75 137 56 755.5 0 56 138 87 755.5 0 87 139 89 755.5 0 89 140 53 755.5 0 53 141 54 755.5 0 54 142 66 755.5 0 66 143 106 755.5 0 106 144 83 755.5 0 83 145 64 755.5 0 64 146 101 755.5 0 101 147 42 755.5 0 42 148 72 755.5 0 72 149 50 755.5 0 50 150 79 755.5 0 79 151 93 755.5 0 93 152 62 755.5 0 62 153 40 755.5 0 40 154 64 755.5 0 64 155 68 755.5 0 68 156 98 755.5 0 98 157 64 755.5 0 64 158 86 755.5 0 86 159 80 755.5 0 80 160 95 755.5 0 95 161 102 755.5 0 102 162 79 755.5 0 79 163 94 755.5 0 94 164 136 755.5 0 136 165 134 755.5 0 134 166 391 755.5 0 391 167 435 755.5 0 435 168 106 755.5 0 106 169 76 755.5 0 76 170 114 755.5 0 114 171 76 755.5 0 76 172 121 755.5 0 121 173 65 755.5 0 65 174 59 755.5 0 59 175 62 755.5 0 62 176 140 755.5 0 140 177 99 755.5 0 99 178 85 755.5 0 85 179 115 755.5 0 115 180 86 755.5 0 86 181 174 755.5 0 174 182 105 755.5 0 105 183 107 755.5 0 107 184 102 755.5 0 102 185 127 755.5 0 127 186 236 755.5 0 236 187 158 755.5 0 158 188 163 755.5 0 163 189 2128 755.5 0 2128 190 551 755.5 0 551 191 769 755.5 0 769 192 113 755.5 0 113 193 95 755.5 0 95 194 156 755.5 0 156 195 99 755.5 0 99 196 117 755.5 0 117 197 122 755.5 0 122 198 70 755.5 0 70 199 63 755.5 0 63 200 112 755.5 0 112 201 114 755.5 0 114 202 120 755.5 0 120 203 113 755.5 0 113 204 69 755.5 0 69 205 82 755.5 0 82 206 107 755.5 0 107 207 103 755.5 0 103 208 117 755.5 0 117 209 121 755.5 0 121 210 67 755.5 0 67 211 144 755.5 0 144 212 143 755.5 0 143 213 119 755.5 0 119 214 105 755.5 0 105 215 88 755.5 0 88 216 137 755.5 0 137 217 161 755.5 0 161 218 97 755.5 0 97 219 136 755.5 0 136 220 216 755.5 0 216 221 126 755.5 0 126 222 83 755.5 0 83 223 36 755.5 0 36 224 55 755.5 0 55 225 133 755.5 0 133 226 145 755.5 0 145 227 115 755.5 0 115 228 130 755.5 0 130 229 110 755.5 0 110 230 143 755.5 0 143 231 143 755.5 0 143 232 157 755.5 0 157 233 302 755.5 0 302 234 184 755.5 0 184 235 97 755.5 0 97 236 687 755.5 0 687 237 391 755.5 0 391 238 217 755.5 0 217 239 137 755.5 0 137 240 116 755.5 0 116 241 110 755.5 0 110 242 83 755.5 0 83 243 85 755.5 0 85 244 106 755.5 0 106 245 992 755.5 0 992 246 2397 755.5 0 2397 247 2281 755.5 0 2281 248 161 755.5 0 161 249 147 755.5 0 147 250 108 755.5 0 108 251 40 755.5 0 40 252 32 755.5 0 32 253 37 755.5 0 37 254 77 755.5 0 77 255 166 755.5 0 166 256 365 755.5 0 365 257 193 755.5 0 193 258 134 755.5 0 134 259 259 755.5 0 259 260 612 755.5 0 612 261 248 755.5 0 248 262 221 755.5 0 221 263 72 755.5 0 72 264 79 755.5 0 79 265 108 755.5 0 108 266 146 755.5 0 146 267 483 755.5 0 483 268 387 755.5 0 387 269 249 755.5 0 249 270 256 755.5 0 256 271 194 755.5 0 194 272 400 755.5 0 400 273 88 755.5 0 88 274 102 755.5 0 102 275 143 755.5 0 143 276 143 755.5 0 143 277 124 755.5 0 124 278 172 755.5 0 172 279 239 755.5 0 239 280 157 755.5 0 157 281 161 755.5 0 161 282 146 755.5 0 146 283 150 755.5 0 150 284 371 755.5 0 371 285 125 755.5 0 125 286 117 755.5 0 117 287 149 755.5 0 149 288 173 755.5 0 173 289 101 755.5 0 101 290 145 755.5 0 145 291 99 755.5 0 99 292 89 755.5 0 89 293 124 755.5 0 124 294 226 755.5 0 226 295 645 755.5 0 645 296 270 755.5 0 270 297 87 755.5 0 87 298 60 755.5 0 60 299 78 755.5 0 78 300 93 755.5 0 93 301 16 755.5 0 16 Finished in 3.56 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 68,106 Reads with adapters: 35,613 (52.3%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 35,602 (52.3%) Total basepairs processed: 17,574,031 bp Total written (filtered): 9,685,472 bp (55.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35613 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 10 0.0 3 0 0 9 1 19 453 0.0 3 0 431 15 2 5 20 34877 0.0 4 33757 899 144 52 25 21 113 0.0 4 27 83 2 0 1 22 123 0.0 4 111 1 9 1 1 23 15 0.0 4 1 0 1 9 4 24 1 0.0 4 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 38 1 0.0 4 1 42 1 0.0 4 1 43 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 222 2 0.0 4 0 0 0 0 2 228 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.55 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 35,602 Reads with adapters: 7,325 (20.6%) Reads written (passing filters): 35,602 (100.0%) Total basepairs processed: 9,685,472 bp Total written (filtered): 9,582,480 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7325 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 1.2% G: 93.4% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 23 556.3 0 23 4 11 139.1 0 11 6 60 8.7 0 60 7 1 2.2 0 1 8 3 0.5 0 3 9 369 0.1 0 30 339 10 121 0.0 1 5 116 11 6635 0.0 1 15 6620 12 4 0.0 1 0 4 14 2 0.0 1 0 2 259 88 0.0 1 86 2 260 3 0.0 1 3 263 3 0.0 1 3 267 1 0.0 1 1 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.