This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 97.81 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 62,201 (2.0%) Reads that were too short: 3,094 (0.1%) Reads written (passing filters): 59,107 (1.9%) Total basepairs processed: 931,490,553 bp Total written (filtered): 14,382,261 bp (1.5%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 62201 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 460 755.5 0 460 7 911 755.5 0 911 8 34856 755.5 0 34856 9 113 755.5 0 113 10 11 755.5 0 11 11 4 755.5 0 4 12 10 755.5 0 10 13 3 755.5 0 3 14 6 755.5 0 6 15 5 755.5 0 5 16 8 755.5 0 8 17 7 755.5 0 7 18 5 755.5 0 5 19 3 755.5 0 3 20 7 755.5 0 7 21 7 755.5 0 7 22 6 755.5 0 6 23 6 755.5 0 6 24 4 755.5 0 4 25 10 755.5 0 10 26 8 755.5 0 8 27 9 755.5 0 9 28 8 755.5 0 8 29 10 755.5 0 10 30 6 755.5 0 6 31 5 755.5 0 5 32 12 755.5 0 12 33 2 755.5 0 2 34 16 755.5 0 16 35 19 755.5 0 19 36 12 755.5 0 12 37 151 755.5 0 151 38 24 755.5 0 24 39 34 755.5 0 34 40 33 755.5 0 33 41 7 755.5 0 7 42 96 755.5 0 96 43 4 755.5 0 4 44 3 755.5 0 3 45 10 755.5 0 10 46 12 755.5 0 12 47 6 755.5 0 6 48 6 755.5 0 6 49 5 755.5 0 5 50 8 755.5 0 8 51 8 755.5 0 8 52 243 755.5 0 243 53 29 755.5 0 29 54 62 755.5 0 62 55 2 755.5 0 2 56 11 755.5 0 11 57 15 755.5 0 15 58 10 755.5 0 10 59 167 755.5 0 167 60 504 755.5 0 504 61 75 755.5 0 75 62 50 755.5 0 50 63 3 755.5 0 3 64 4 755.5 0 4 65 9 755.5 0 9 66 15 755.5 0 15 67 8 755.5 0 8 68 5 755.5 0 5 70 2 755.5 0 2 71 17 755.5 0 17 72 35 755.5 0 35 73 201 755.5 0 201 74 3 755.5 0 3 75 6 755.5 0 6 76 4 755.5 0 4 77 4 755.5 0 4 78 4 755.5 0 4 79 3 755.5 0 3 80 7 755.5 0 7 81 9 755.5 0 9 82 5 755.5 0 5 83 11 755.5 0 11 84 25 755.5 0 25 85 12 755.5 0 12 86 15 755.5 0 15 87 6 755.5 0 6 88 2 755.5 0 2 89 7 755.5 0 7 90 14 755.5 0 14 91 8 755.5 0 8 92 6 755.5 0 6 93 12 755.5 0 12 94 10 755.5 0 10 95 26 755.5 0 26 96 7 755.5 0 7 97 6 755.5 0 6 98 3 755.5 0 3 99 3 755.5 0 3 100 1 755.5 0 1 101 4 755.5 0 4 102 4 755.5 0 4 103 8 755.5 0 8 104 11 755.5 0 11 105 18 755.5 0 18 106 8 755.5 0 8 107 13 755.5 0 13 108 25 755.5 0 25 109 10 755.5 0 10 110 19 755.5 0 19 111 18 755.5 0 18 112 73 755.5 0 73 113 10 755.5 0 10 114 8 755.5 0 8 115 12 755.5 0 12 116 13 755.5 0 13 117 39 755.5 0 39 118 19 755.5 0 19 119 17 755.5 0 17 120 22 755.5 0 22 121 23 755.5 0 23 122 9 755.5 0 9 123 9 755.5 0 9 124 33 755.5 0 33 125 15 755.5 0 15 126 31 755.5 0 31 127 262 755.5 0 262 128 44 755.5 0 44 129 46 755.5 0 46 130 12 755.5 0 12 131 25 755.5 0 25 132 312 755.5 0 312 133 27 755.5 0 27 134 25 755.5 0 25 135 19 755.5 0 19 136 9 755.5 0 9 137 34 755.5 0 34 138 30 755.5 0 30 139 59 755.5 0 59 140 76 755.5 0 76 141 77 755.5 0 77 142 236 755.5 0 236 143 652 755.5 0 652 144 11884 755.5 0 11884 145 1622 755.5 0 1622 146 2549 755.5 0 2549 147 21 755.5 0 21 148 15 755.5 0 15 149 245 755.5 0 245 150 7 755.5 0 7 151 82 755.5 0 82 152 23 755.5 0 23 153 10 755.5 0 10 154 17 755.5 0 17 155 27 755.5 0 27 156 56 755.5 0 56 157 28 755.5 0 28 158 28 755.5 0 28 159 33 755.5 0 33 160 4 755.5 0 4 161 13 755.5 0 13 162 6 755.5 0 6 163 4 755.5 0 4 164 7 755.5 0 7 165 15 755.5 0 15 166 21 755.5 0 21 167 9 755.5 0 9 168 6 755.5 0 6 169 7 755.5 0 7 170 18 755.5 0 18 171 39 755.5 0 39 172 78 755.5 0 78 173 60 755.5 0 60 174 8 755.5 0 8 175 5 755.5 0 5 176 10 755.5 0 10 177 24 755.5 0 24 178 35 755.5 0 35 179 79 755.5 0 79 180 23 755.5 0 23 181 24 755.5 0 24 182 14 755.5 0 14 183 8 755.5 0 8 184 14 755.5 0 14 185 17 755.5 0 17 186 15 755.5 0 15 187 6 755.5 0 6 188 9 755.5 0 9 189 24 755.5 0 24 190 214 755.5 0 214 191 29 755.5 0 29 192 208 755.5 0 208 193 15 755.5 0 15 194 15 755.5 0 15 195 8 755.5 0 8 196 26 755.5 0 26 197 59 755.5 0 59 198 156 755.5 0 156 199 55 755.5 0 55 200 8 755.5 0 8 201 7 755.5 0 7 202 6 755.5 0 6 204 15 755.5 0 15 205 14 755.5 0 14 206 16 755.5 0 16 207 13 755.5 0 13 208 17 755.5 0 17 209 3 755.5 0 3 210 1 755.5 0 1 211 10 755.5 0 10 212 14 755.5 0 14 213 11 755.5 0 11 214 12 755.5 0 12 215 5 755.5 0 5 216 13 755.5 0 13 217 17 755.5 0 17 218 11 755.5 0 11 219 3 755.5 0 3 220 14 755.5 0 14 222 4 755.5 0 4 223 5 755.5 0 5 224 11 755.5 0 11 225 17 755.5 0 17 226 23 755.5 0 23 227 19 755.5 0 19 228 38 755.5 0 38 229 74 755.5 0 74 230 43 755.5 0 43 231 14 755.5 0 14 232 11 755.5 0 11 233 11 755.5 0 11 234 9 755.5 0 9 235 23 755.5 0 23 236 112 755.5 0 112 237 43 755.5 0 43 238 42 755.5 0 42 239 9 755.5 0 9 240 33 755.5 0 33 241 12 755.5 0 12 242 25 755.5 0 25 243 5 755.5 0 5 244 7 755.5 0 7 245 10 755.5 0 10 246 15 755.5 0 15 247 23 755.5 0 23 248 7 755.5 0 7 249 9 755.5 0 9 250 2 755.5 0 2 251 12 755.5 0 12 252 21 755.5 0 21 253 10 755.5 0 10 254 26 755.5 0 26 255 20 755.5 0 20 256 46 755.5 0 46 257 12 755.5 0 12 258 13 755.5 0 13 259 11 755.5 0 11 260 119 755.5 0 119 261 196 755.5 0 196 262 426 755.5 0 426 263 43 755.5 0 43 264 25 755.5 0 25 265 30 755.5 0 30 266 13 755.5 0 13 267 35 755.5 0 35 268 12 755.5 0 12 269 9 755.5 0 9 270 5 755.5 0 5 271 10 755.5 0 10 272 13 755.5 0 13 273 4 755.5 0 4 274 5 755.5 0 5 275 7 755.5 0 7 276 7 755.5 0 7 277 15 755.5 0 15 278 9 755.5 0 9 279 10 755.5 0 10 280 10 755.5 0 10 281 6 755.5 0 6 282 16 755.5 0 16 283 191 755.5 0 191 284 343 755.5 0 343 285 212 755.5 0 212 286 50 755.5 0 50 287 19 755.5 0 19 288 27 755.5 0 27 289 14 755.5 0 14 290 15 755.5 0 15 291 43 755.5 0 43 292 28 755.5 0 28 293 51 755.5 0 51 294 22 755.5 0 22 295 14 755.5 0 14 296 3 755.5 0 3 297 2 755.5 0 2 298 1 755.5 0 1 299 3 755.5 0 3 300 1 755.5 0 1 301 8 755.5 0 8 Finished in 2.68 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 59,107 Reads with adapters: 36,058 (61.0%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 36,054 (61.0%) Total basepairs processed: 14,382,261 bp Total written (filtered): 9,951,013 bp (69.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36058 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 7 1 16 264 0.0 3 0 260 4 17 35586 0.0 3 35063 509 13 1 18 79 0.0 3 2 73 3 1 19 88 0.0 3 0 0 85 3 20 16 0.0 3 0 0 0 16 27 1 0.0 3 1 38 1 0.0 3 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 1 95 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 154 2 0.0 3 1 0 0 1 182 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.76 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 36,054 Reads with adapters: 10,713 (29.7%) Reads written (passing filters): 36,054 (100.0%) Total basepairs processed: 9,951,013 bp Total written (filtered): 9,918,414 bp (99.7%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 10713 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 2.9% C: 0.2% G: 96.4% T: 0.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10253 563.3 0 10253 4 460 140.8 0 460 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.