This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.49 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 376,928 (12.2%) Reads that were too short: 175,063 (5.7%) Reads written (passing filters): 201,865 (6.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 44,969,475 bp (4.8%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 376928 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 903 755.5 0 903 7 4616 755.5 0 4616 8 85058 755.5 0 85058 9 213 755.5 0 213 10 158 755.5 0 158 11 6114 755.5 0 6114 12 27 755.5 0 27 13 17 755.5 0 17 14 15 755.5 0 15 15 29 755.5 0 29 16 11 755.5 0 11 17 15 755.5 0 15 18 22 755.5 0 22 19 27 755.5 0 27 20 16 755.5 0 16 21 15 755.5 0 15 22 19 755.5 0 19 23 27 755.5 0 27 24 22 755.5 0 22 25 15 755.5 0 15 26 11 755.5 0 11 27 17 755.5 0 17 28 21 755.5 0 21 29 13 755.5 0 13 30 20 755.5 0 20 31 22 755.5 0 22 32 26 755.5 0 26 33 9 755.5 0 9 34 25 755.5 0 25 35 23 755.5 0 23 36 21 755.5 0 21 37 17 755.5 0 17 38 14 755.5 0 14 39 20 755.5 0 20 40 16 755.5 0 16 41 16 755.5 0 16 42 23 755.5 0 23 43 21 755.5 0 21 44 24 755.5 0 24 45 12 755.5 0 12 46 10 755.5 0 10 47 18 755.5 0 18 48 16 755.5 0 16 49 16 755.5 0 16 50 14 755.5 0 14 51 16 755.5 0 16 52 23 755.5 0 23 53 18 755.5 0 18 54 37 755.5 0 37 55 20 755.5 0 20 56 20 755.5 0 20 57 16 755.5 0 16 58 28 755.5 0 28 59 30 755.5 0 30 60 21 755.5 0 21 61 11 755.5 0 11 62 17 755.5 0 17 63 22 755.5 0 22 64 54 755.5 0 54 65 31 755.5 0 31 66 22 755.5 0 22 67 28 755.5 0 28 68 77 755.5 0 77 69 37 755.5 0 37 70 108 755.5 0 108 71 109 755.5 0 109 72 782 755.5 0 782 73 446 755.5 0 446 74 699 755.5 0 699 75 744 755.5 0 744 76 277 755.5 0 277 77 513 755.5 0 513 78 848 755.5 0 848 79 464 755.5 0 464 80 613 755.5 0 613 81 413 755.5 0 413 82 340 755.5 0 340 83 266 755.5 0 266 84 546 755.5 0 546 85 219 755.5 0 219 86 406 755.5 0 406 87 638 755.5 0 638 88 2399 755.5 0 2399 89 155 755.5 0 155 90 260 755.5 0 260 91 1681 755.5 0 1681 92 431 755.5 0 431 93 1035 755.5 0 1035 94 454 755.5 0 454 95 220 755.5 0 220 96 109 755.5 0 109 97 272 755.5 0 272 98 425 755.5 0 425 99 300 755.5 0 300 100 262 755.5 0 262 101 699 755.5 0 699 102 337 755.5 0 337 103 632 755.5 0 632 104 1773 755.5 0 1773 105 625 755.5 0 625 106 190 755.5 0 190 107 567 755.5 0 567 108 658 755.5 0 658 109 385 755.5 0 385 110 372 755.5 0 372 111 463 755.5 0 463 112 271 755.5 0 271 113 501 755.5 0 501 114 297 755.5 0 297 115 329 755.5 0 329 116 553 755.5 0 553 117 1303 755.5 0 1303 118 422 755.5 0 422 119 819 755.5 0 819 120 994 755.5 0 994 121 783 755.5 0 783 122 722 755.5 0 722 123 1387 755.5 0 1387 124 1101 755.5 0 1101 125 1028 755.5 0 1028 126 1402 755.5 0 1402 127 352 755.5 0 352 128 416 755.5 0 416 129 600 755.5 0 600 130 936 755.5 0 936 131 705 755.5 0 705 132 1325 755.5 0 1325 133 796 755.5 0 796 134 782 755.5 0 782 135 729 755.5 0 729 136 734 755.5 0 734 137 678 755.5 0 678 138 595 755.5 0 595 139 707 755.5 0 707 140 350 755.5 0 350 141 554 755.5 0 554 142 799 755.5 0 799 143 1302 755.5 0 1302 144 534 755.5 0 534 145 746 755.5 0 746 146 708 755.5 0 708 147 792 755.5 0 792 148 745 755.5 0 745 149 874 755.5 0 874 150 1121 755.5 0 1121 151 982 755.5 0 982 152 1163 755.5 0 1163 153 684 755.5 0 684 154 795 755.5 0 795 155 1128 755.5 0 1128 156 916 755.5 0 916 157 957 755.5 0 957 158 927 755.5 0 927 159 751 755.5 0 751 160 888 755.5 0 888 161 719 755.5 0 719 162 871 755.5 0 871 163 1030 755.5 0 1030 164 873 755.5 0 873 165 674 755.5 0 674 166 780 755.5 0 780 167 877 755.5 0 877 168 853 755.5 0 853 169 856 755.5 0 856 170 1464 755.5 0 1464 171 2692 755.5 0 2692 172 1486 755.5 0 1486 173 635 755.5 0 635 174 808 755.5 0 808 175 697 755.5 0 697 176 937 755.5 0 937 177 894 755.5 0 894 178 1623 755.5 0 1623 179 1345 755.5 0 1345 180 1184 755.5 0 1184 181 721 755.5 0 721 182 952 755.5 0 952 183 1044 755.5 0 1044 184 785 755.5 0 785 185 603 755.5 0 603 186 507 755.5 0 507 187 916 755.5 0 916 188 1734 755.5 0 1734 189 1567 755.5 0 1567 190 1218 755.5 0 1218 191 909 755.5 0 909 192 840 755.5 0 840 193 662 755.5 0 662 194 699 755.5 0 699 195 2089 755.5 0 2089 196 1261 755.5 0 1261 197 445 755.5 0 445 198 539 755.5 0 539 199 567 755.5 0 567 200 919 755.5 0 919 201 1160 755.5 0 1160 202 875 755.5 0 875 203 681 755.5 0 681 204 732 755.5 0 732 205 587 755.5 0 587 206 340 755.5 0 340 207 765 755.5 0 765 208 1044 755.5 0 1044 209 587 755.5 0 587 210 459 755.5 0 459 211 763 755.5 0 763 212 552 755.5 0 552 213 671 755.5 0 671 214 1550 755.5 0 1550 215 2185 755.5 0 2185 216 1439 755.5 0 1439 217 1267 755.5 0 1267 218 847 755.5 0 847 219 458 755.5 0 458 220 542 755.5 0 542 221 1666 755.5 0 1666 222 1497 755.5 0 1497 223 974 755.5 0 974 224 1132 755.5 0 1132 225 1813 755.5 0 1813 226 4771 755.5 0 4771 227 9542 755.5 0 9542 228 1814 755.5 0 1814 229 3071 755.5 0 3071 230 1675 755.5 0 1675 231 3264 755.5 0 3264 232 2211 755.5 0 2211 233 2601 755.5 0 2601 234 637 755.5 0 637 235 465 755.5 0 465 236 798 755.5 0 798 237 899 755.5 0 899 238 936 755.5 0 936 239 945 755.5 0 945 240 888 755.5 0 888 241 958 755.5 0 958 242 1334 755.5 0 1334 243 1004 755.5 0 1004 244 822 755.5 0 822 245 612 755.5 0 612 246 480 755.5 0 480 247 588 755.5 0 588 248 1282 755.5 0 1282 249 1985 755.5 0 1985 250 2029 755.5 0 2029 251 1775 755.5 0 1775 252 2274 755.5 0 2274 253 2142 755.5 0 2142 254 1076 755.5 0 1076 255 889 755.5 0 889 256 620 755.5 0 620 257 948 755.5 0 948 258 725 755.5 0 725 259 901 755.5 0 901 260 855 755.5 0 855 261 1331 755.5 0 1331 262 1669 755.5 0 1669 263 2552 755.5 0 2552 264 8355 755.5 0 8355 265 3197 755.5 0 3197 266 4212 755.5 0 4212 267 882 755.5 0 882 268 1560 755.5 0 1560 269 1070 755.5 0 1070 270 898 755.5 0 898 271 1287 755.5 0 1287 272 1296 755.5 0 1296 273 1887 755.5 0 1887 274 3705 755.5 0 3705 275 2630 755.5 0 2630 276 2263 755.5 0 2263 277 1811 755.5 0 1811 278 1268 755.5 0 1268 279 797 755.5 0 797 280 1236 755.5 0 1236 281 2704 755.5 0 2704 282 1824 755.5 0 1824 283 1288 755.5 0 1288 284 1737 755.5 0 1737 285 1185 755.5 0 1185 286 909 755.5 0 909 287 980 755.5 0 980 288 777 755.5 0 777 289 1215 755.5 0 1215 290 1066 755.5 0 1066 291 3397 755.5 0 3397 292 5305 755.5 0 5305 293 23122 755.5 0 23122 294 651 755.5 0 651 295 524 755.5 0 524 296 595 755.5 0 595 297 653 755.5 0 653 298 415 755.5 0 415 299 467 755.5 0 467 300 577 755.5 0 577 301 524 755.5 0 524 Finished in 9.42 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 201,865 Reads with adapters: 87,863 (43.5%) Reads that were too short: 40 (0.0%) Reads written (passing filters): 87,823 (43.5%) Total basepairs processed: 44,969,475 bp Total written (filtered): 23,969,998 bp (53.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 87863 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 42 0.0 3 0 0 35 6 1 19 1080 0.0 3 0 977 50 38 15 20 86319 0.0 4 83820 2228 210 43 18 21 223 0.0 4 36 176 9 1 1 22 10 0.0 4 0 2 8 23 53 0.0 4 0 0 0 40 13 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 0 0 0 0 1 26 2 0.0 4 0 0 0 1 1 32 3 0.0 4 1 0 0 0 2 34 1 0.0 4 1 36 4 0.0 4 0 0 0 0 4 41 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 1 46 2 0.0 4 1 0 0 0 1 54 1 0.0 4 0 0 0 0 1 58 3 0.0 4 0 0 0 0 3 59 2 0.0 4 1 0 0 0 1 63 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 84 2 0.0 4 0 0 0 0 2 88 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 100 1 0.0 4 1 102 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 2 0.0 4 0 0 0 0 2 127 61 0.0 4 0 0 0 0 61 133 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 1 167 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 280 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.50 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 87,823 Reads with adapters: 2,742 (3.1%) Reads written (passing filters): 87,823 (100.0%) Total basepairs processed: 23,969,998 bp Total written (filtered): 23,938,609 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2742 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 2.3% G: 93.1% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 1372.2 0 7 4 2 343.1 0 2 5 6 85.8 0 6 9 4 0.3 0 0 4 10 270 0.1 1 8 262 11 2374 0.0 1 4 2370 12 61 0.0 1 5 56 13 1 0.0 1 0 1 14 1 0.0 1 0 1 15 9 0.0 1 0 9 151 1 0.0 1 1 209 1 0.0 1 1 211 2 0.0 1 2 263 1 0.0 1 1 268 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.