This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 100.17 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 117,684 (3.8%) Reads that were too short: 5,950 (0.2%) Reads written (passing filters): 111,734 (3.6%) Total basepairs processed: 931,490,553 bp Total written (filtered): 30,989,766 bp (3.3%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 117684 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1112 755.5 0 1112 7 89450 755.5 0 89450 8 96 755.5 0 96 9 10 755.5 0 10 10 5 755.5 0 5 11 11 755.5 0 11 12 10 755.5 0 10 13 3 755.5 0 3 14 10 755.5 0 10 15 6 755.5 0 6 16 6 755.5 0 6 17 18 755.5 0 18 18 34 755.5 0 34 19 21 755.5 0 21 20 29 755.5 0 29 21 8 755.5 0 8 22 9 755.5 0 9 23 15 755.5 0 15 24 7 755.5 0 7 25 12 755.5 0 12 26 8 755.5 0 8 27 7 755.5 0 7 28 2 755.5 0 2 29 8 755.5 0 8 30 16 755.5 0 16 31 33 755.5 0 33 32 13 755.5 0 13 33 9 755.5 0 9 34 19 755.5 0 19 35 12 755.5 0 12 36 11 755.5 0 11 37 19 755.5 0 19 38 12 755.5 0 12 39 6 755.5 0 6 40 13 755.5 0 13 41 13 755.5 0 13 42 6 755.5 0 6 43 10 755.5 0 10 44 39 755.5 0 39 45 15 755.5 0 15 46 14 755.5 0 14 47 14 755.5 0 14 48 56 755.5 0 56 49 10 755.5 0 10 50 75 755.5 0 75 51 13 755.5 0 13 52 11 755.5 0 11 53 29 755.5 0 29 54 60 755.5 0 60 55 17 755.5 0 17 56 20 755.5 0 20 57 38 755.5 0 38 58 20 755.5 0 20 59 46 755.5 0 46 60 45 755.5 0 45 61 42 755.5 0 42 62 10 755.5 0 10 63 21 755.5 0 21 64 37 755.5 0 37 65 32 755.5 0 32 66 53 755.5 0 53 67 83 755.5 0 83 68 92 755.5 0 92 69 137 755.5 0 137 70 160 755.5 0 160 71 1734 755.5 0 1734 72 7539 755.5 0 7539 73 1195 755.5 0 1195 74 13 755.5 0 13 75 3 755.5 0 3 76 6 755.5 0 6 77 32 755.5 0 32 78 38 755.5 0 38 79 64 755.5 0 64 80 14 755.5 0 14 81 16 755.5 0 16 82 10 755.5 0 10 83 14 755.5 0 14 84 12 755.5 0 12 85 9 755.5 0 9 86 6 755.5 0 6 87 16 755.5 0 16 88 16 755.5 0 16 89 29 755.5 0 29 90 7 755.5 0 7 91 53 755.5 0 53 92 69 755.5 0 69 93 288 755.5 0 288 94 92 755.5 0 92 95 156 755.5 0 156 96 857 755.5 0 857 97 576 755.5 0 576 98 1555 755.5 0 1555 99 81 755.5 0 81 100 26 755.5 0 26 101 17 755.5 0 17 102 5 755.5 0 5 103 7 755.5 0 7 104 18 755.5 0 18 105 15 755.5 0 15 106 9 755.5 0 9 107 10 755.5 0 10 108 16 755.5 0 16 109 15 755.5 0 15 110 10 755.5 0 10 111 5 755.5 0 5 112 16 755.5 0 16 113 5 755.5 0 5 114 23 755.5 0 23 115 37 755.5 0 37 116 33 755.5 0 33 117 51 755.5 0 51 118 29 755.5 0 29 119 33 755.5 0 33 120 16 755.5 0 16 121 28 755.5 0 28 122 20 755.5 0 20 123 33 755.5 0 33 124 45 755.5 0 45 125 74 755.5 0 74 126 520 755.5 0 520 127 29 755.5 0 29 128 23 755.5 0 23 129 50 755.5 0 50 130 68 755.5 0 68 131 33 755.5 0 33 132 133 755.5 0 133 133 36 755.5 0 36 134 33 755.5 0 33 135 75 755.5 0 75 136 62 755.5 0 62 137 41 755.5 0 41 138 40 755.5 0 40 139 20 755.5 0 20 140 25 755.5 0 25 141 25 755.5 0 25 142 22 755.5 0 22 143 29 755.5 0 29 144 48 755.5 0 48 145 84 755.5 0 84 146 32 755.5 0 32 147 6 755.5 0 6 148 9 755.5 0 9 149 7 755.5 0 7 150 21 755.5 0 21 151 17 755.5 0 17 152 11 755.5 0 11 153 19 755.5 0 19 154 41 755.5 0 41 155 74 755.5 0 74 156 74 755.5 0 74 157 46 755.5 0 46 158 41 755.5 0 41 159 29 755.5 0 29 160 24 755.5 0 24 161 20 755.5 0 20 162 28 755.5 0 28 163 49 755.5 0 49 164 42 755.5 0 42 165 62 755.5 0 62 166 41 755.5 0 41 167 40 755.5 0 40 168 26 755.5 0 26 169 25 755.5 0 25 170 22 755.5 0 22 171 39 755.5 0 39 172 74 755.5 0 74 173 84 755.5 0 84 174 64 755.5 0 64 175 29 755.5 0 29 176 12 755.5 0 12 177 23 755.5 0 23 178 31 755.5 0 31 179 47 755.5 0 47 180 59 755.5 0 59 181 35 755.5 0 35 182 39 755.5 0 39 183 38 755.5 0 38 184 52 755.5 0 52 185 42 755.5 0 42 186 26 755.5 0 26 187 18 755.5 0 18 188 17 755.5 0 17 189 35 755.5 0 35 190 132 755.5 0 132 191 191 755.5 0 191 192 666 755.5 0 666 193 97 755.5 0 97 194 135 755.5 0 135 195 29 755.5 0 29 196 32 755.5 0 32 197 38 755.5 0 38 198 19 755.5 0 19 199 16 755.5 0 16 200 20 755.5 0 20 201 19 755.5 0 19 202 44 755.5 0 44 203 109 755.5 0 109 204 149 755.5 0 149 205 50 755.5 0 50 206 32 755.5 0 32 207 15 755.5 0 15 208 23 755.5 0 23 209 35 755.5 0 35 210 89 755.5 0 89 211 139 755.5 0 139 212 87 755.5 0 87 213 42 755.5 0 42 214 23 755.5 0 23 215 50 755.5 0 50 216 129 755.5 0 129 217 70 755.5 0 70 218 40 755.5 0 40 219 32 755.5 0 32 220 37 755.5 0 37 221 71 755.5 0 71 222 129 755.5 0 129 223 47 755.5 0 47 224 53 755.5 0 53 225 74 755.5 0 74 226 247 755.5 0 247 227 88 755.5 0 88 228 35 755.5 0 35 229 26 755.5 0 26 230 16 755.5 0 16 231 19 755.5 0 19 232 28 755.5 0 28 233 38 755.5 0 38 234 35 755.5 0 35 235 48 755.5 0 48 236 57 755.5 0 57 237 46 755.5 0 46 238 27 755.5 0 27 239 18 755.5 0 18 240 18 755.5 0 18 241 18 755.5 0 18 242 48 755.5 0 48 243 54 755.5 0 54 244 46 755.5 0 46 245 53 755.5 0 53 246 32 755.5 0 32 247 42 755.5 0 42 248 8 755.5 0 8 249 17 755.5 0 17 250 24 755.5 0 24 251 94 755.5 0 94 252 44 755.5 0 44 253 52 755.5 0 52 254 52 755.5 0 52 255 71 755.5 0 71 256 44 755.5 0 44 257 38 755.5 0 38 258 35 755.5 0 35 259 26 755.5 0 26 260 28 755.5 0 28 261 78 755.5 0 78 262 54 755.5 0 54 263 58 755.5 0 58 264 72 755.5 0 72 265 75 755.5 0 75 266 95 755.5 0 95 267 20 755.5 0 20 268 23 755.5 0 23 269 22 755.5 0 22 270 61 755.5 0 61 271 306 755.5 0 306 272 110 755.5 0 110 273 59 755.5 0 59 274 37 755.5 0 37 275 18 755.5 0 18 276 17 755.5 0 17 277 33 755.5 0 33 278 149 755.5 0 149 279 328 755.5 0 328 280 153 755.5 0 153 281 17 755.5 0 17 282 30 755.5 0 30 283 30 755.5 0 30 284 58 755.5 0 58 285 72 755.5 0 72 286 49 755.5 0 49 287 23 755.5 0 23 288 12 755.5 0 12 289 34 755.5 0 34 290 70 755.5 0 70 291 108 755.5 0 108 292 50 755.5 0 50 293 27 755.5 0 27 294 16 755.5 0 16 295 18 755.5 0 18 296 29 755.5 0 29 297 80 755.5 0 80 298 76 755.5 0 76 299 100 755.5 0 100 300 95 755.5 0 95 301 47 755.5 0 47 Finished in 5.24 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 111,734 Reads with adapters: 90,418 (80.9%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 90,410 (80.9%) Total basepairs processed: 30,989,766 bp Total written (filtered): 25,042,776 bp (80.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 90418 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 13 0.0 3 0 0 12 1 16 528 0.0 3 0 514 13 1 17 89652 0.0 3 88112 1494 42 4 18 141 0.0 3 9 127 4 1 19 39 0.0 3 0 0 32 7 20 17 0.0 3 0 0 0 17 21 1 0.0 3 1 24 1 0.0 3 1 25 1 0.0 3 0 0 0 1 32 2 0.0 3 1 0 0 1 38 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.25 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 90,410 Reads with adapters: 1,224 (1.4%) Reads written (passing filters): 90,410 (100.0%) Total basepairs processed: 25,042,776 bp Total written (filtered): 25,038,990 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1224 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.5% C: 63.6% G: 15.7% T: 15.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1111 1412.7 0 1111 4 112 353.2 0 112 5 1 88.3 0 1