This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 92.89 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 220,218 (7.1%) Reads that were too short: 23,464 (0.8%) Reads written (passing filters): 196,754 (6.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 51,588,406 bp (5.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 220218 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4446 755.5 0 4446 7 129216 755.5 0 129216 8 180 755.5 0 180 9 7083 755.5 0 7083 10 15 755.5 0 15 11 4 755.5 0 4 12 5 755.5 0 5 13 1 755.5 0 1 14 5 755.5 0 5 15 2 755.5 0 2 16 2 755.5 0 2 17 1 755.5 0 1 18 4 755.5 0 4 19 7 755.5 0 7 20 9 755.5 0 9 21 89 755.5 0 89 22 8 755.5 0 8 23 42 755.5 0 42 24 5 755.5 0 5 25 39 755.5 0 39 26 8 755.5 0 8 27 6 755.5 0 6 28 1 755.5 0 1 29 4 755.5 0 4 30 6 755.5 0 6 31 5 755.5 0 5 32 29 755.5 0 29 33 22 755.5 0 22 34 1445 755.5 0 1445 35 272 755.5 0 272 36 10 755.5 0 10 37 4 755.5 0 4 38 4 755.5 0 4 39 2 755.5 0 2 40 7 755.5 0 7 41 2 755.5 0 2 42 4 755.5 0 4 43 2 755.5 0 2 44 3 755.5 0 3 45 4 755.5 0 4 46 6 755.5 0 6 47 3 755.5 0 3 48 2 755.5 0 2 49 3 755.5 0 3 50 4 755.5 0 4 51 2 755.5 0 2 52 1 755.5 0 1 53 28 755.5 0 28 54 9 755.5 0 9 55 4 755.5 0 4 56 4 755.5 0 4 57 6 755.5 0 6 58 6 755.5 0 6 59 4 755.5 0 4 60 8 755.5 0 8 61 8 755.5 0 8 62 46 755.5 0 46 63 6 755.5 0 6 64 2 755.5 0 2 65 2 755.5 0 2 66 1 755.5 0 1 68 13 755.5 0 13 69 20 755.5 0 20 70 4 755.5 0 4 71 4 755.5 0 4 72 8 755.5 0 8 73 3 755.5 0 3 74 20 755.5 0 20 75 13 755.5 0 13 76 6 755.5 0 6 77 12 755.5 0 12 78 56 755.5 0 56 79 74 755.5 0 74 80 66 755.5 0 66 81 59 755.5 0 59 82 117 755.5 0 117 83 102 755.5 0 102 84 65 755.5 0 65 85 115 755.5 0 115 86 78 755.5 0 78 87 143 755.5 0 143 88 96 755.5 0 96 89 27 755.5 0 27 90 69 755.5 0 69 91 36 755.5 0 36 92 21 755.5 0 21 93 53 755.5 0 53 94 117 755.5 0 117 95 199 755.5 0 199 96 317 755.5 0 317 97 339 755.5 0 339 98 525 755.5 0 525 99 333 755.5 0 333 100 275 755.5 0 275 101 541 755.5 0 541 102 167 755.5 0 167 103 93 755.5 0 93 104 187 755.5 0 187 105 157 755.5 0 157 106 138 755.5 0 138 107 203 755.5 0 203 108 381 755.5 0 381 109 401 755.5 0 401 110 398 755.5 0 398 111 8497 755.5 0 8497 112 4625 755.5 0 4625 113 19500 755.5 0 19500 114 1062 755.5 0 1062 115 369 755.5 0 369 116 95 755.5 0 95 117 52 755.5 0 52 118 53 755.5 0 53 119 66 755.5 0 66 120 99 755.5 0 99 121 82 755.5 0 82 122 56 755.5 0 56 123 57 755.5 0 57 124 59 755.5 0 59 125 67 755.5 0 67 126 76 755.5 0 76 127 100 755.5 0 100 128 108 755.5 0 108 129 291 755.5 0 291 130 199 755.5 0 199 131 472 755.5 0 472 132 176 755.5 0 176 133 245 755.5 0 245 134 167 755.5 0 167 135 127 755.5 0 127 136 108 755.5 0 108 137 86 755.5 0 86 138 116 755.5 0 116 139 133 755.5 0 133 140 147 755.5 0 147 141 114 755.5 0 114 142 162 755.5 0 162 143 194 755.5 0 194 144 1342 755.5 0 1342 145 722 755.5 0 722 146 142 755.5 0 142 147 309 755.5 0 309 148 151 755.5 0 151 149 165 755.5 0 165 150 109 755.5 0 109 151 80 755.5 0 80 152 58 755.5 0 58 153 60 755.5 0 60 154 69 755.5 0 69 155 102 755.5 0 102 156 106 755.5 0 106 157 100 755.5 0 100 158 176 755.5 0 176 159 130 755.5 0 130 160 149 755.5 0 149 161 111 755.5 0 111 162 128 755.5 0 128 163 111 755.5 0 111 164 106 755.5 0 106 165 119 755.5 0 119 166 128 755.5 0 128 167 155 755.5 0 155 168 151 755.5 0 151 169 179 755.5 0 179 170 101 755.5 0 101 171 134 755.5 0 134 172 63 755.5 0 63 173 64 755.5 0 64 174 53 755.5 0 53 175 181 755.5 0 181 176 111 755.5 0 111 177 115 755.5 0 115 178 62 755.5 0 62 179 59 755.5 0 59 180 65 755.5 0 65 181 156 755.5 0 156 182 187 755.5 0 187 183 176 755.5 0 176 184 148 755.5 0 148 185 216 755.5 0 216 186 279 755.5 0 279 187 221 755.5 0 221 188 166 755.5 0 166 189 146 755.5 0 146 190 62 755.5 0 62 191 66 755.5 0 66 192 104 755.5 0 104 193 324 755.5 0 324 194 244 755.5 0 244 195 309 755.5 0 309 196 151 755.5 0 151 197 79 755.5 0 79 198 88 755.5 0 88 199 163 755.5 0 163 200 188 755.5 0 188 201 146 755.5 0 146 202 64 755.5 0 64 203 59 755.5 0 59 204 116 755.5 0 116 205 268 755.5 0 268 206 282 755.5 0 282 207 290 755.5 0 290 208 186 755.5 0 186 209 114 755.5 0 114 210 103 755.5 0 103 211 110 755.5 0 110 212 191 755.5 0 191 213 238 755.5 0 238 214 226 755.5 0 226 215 228 755.5 0 228 216 186 755.5 0 186 217 182 755.5 0 182 218 180 755.5 0 180 219 288 755.5 0 288 220 252 755.5 0 252 221 230 755.5 0 230 222 111 755.5 0 111 223 80 755.5 0 80 224 145 755.5 0 145 225 185 755.5 0 185 226 235 755.5 0 235 227 225 755.5 0 225 228 258 755.5 0 258 229 349 755.5 0 349 230 277 755.5 0 277 231 208 755.5 0 208 232 162 755.5 0 162 233 104 755.5 0 104 234 104 755.5 0 104 235 187 755.5 0 187 236 329 755.5 0 329 237 296 755.5 0 296 238 225 755.5 0 225 239 208 755.5 0 208 240 187 755.5 0 187 241 159 755.5 0 159 242 88 755.5 0 88 243 115 755.5 0 115 244 142 755.5 0 142 245 227 755.5 0 227 246 299 755.5 0 299 247 284 755.5 0 284 248 368 755.5 0 368 249 183 755.5 0 183 250 140 755.5 0 140 251 58 755.5 0 58 252 56 755.5 0 56 253 76 755.5 0 76 254 134 755.5 0 134 255 162 755.5 0 162 256 209 755.5 0 209 257 286 755.5 0 286 258 690 755.5 0 690 259 325 755.5 0 325 260 204 755.5 0 204 261 118 755.5 0 118 262 99 755.5 0 99 263 143 755.5 0 143 264 103 755.5 0 103 265 114 755.5 0 114 266 177 755.5 0 177 267 344 755.5 0 344 268 456 755.5 0 456 269 509 755.5 0 509 270 341 755.5 0 341 271 133 755.5 0 133 272 134 755.5 0 134 273 183 755.5 0 183 274 254 755.5 0 254 275 284 755.5 0 284 276 338 755.5 0 338 277 205 755.5 0 205 278 105 755.5 0 105 279 185 755.5 0 185 280 324 755.5 0 324 281 419 755.5 0 419 282 711 755.5 0 711 283 263 755.5 0 263 284 390 755.5 0 390 285 107 755.5 0 107 286 172 755.5 0 172 287 202 755.5 0 202 288 349 755.5 0 349 289 190 755.5 0 190 290 315 755.5 0 315 291 269 755.5 0 269 292 689 755.5 0 689 293 330 755.5 0 330 294 424 755.5 0 424 295 367 755.5 0 367 296 280 755.5 0 280 297 146 755.5 0 146 298 232 755.5 0 232 299 243 755.5 0 243 300 591 755.5 0 591 301 353 755.5 0 353 Finished in 9.08 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 196,754 Reads with adapters: 133,506 (67.9%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 133,492 (67.8%) Total basepairs processed: 51,588,406 bp Total written (filtered): 36,571,241 bp (70.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 133506 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 3 0.0 3 0 0 0 1 2 18 37 0.0 3 0 0 23 6 8 19 882 0.0 3 0 813 36 20 13 20 132087 0.0 4 129306 2383 244 95 59 21 299 0.0 4 46 229 9 4 11 22 25 0.0 4 6 0 18 0 1 23 68 0.0 4 0 0 0 59 9 24 2 0.0 4 1 0 0 0 1 26 2 0.0 4 0 0 0 0 2 29 1 0.0 4 0 0 0 0 1 31 2 0.0 4 0 0 0 0 2 35 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 42 1 0.0 4 1 57 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 105 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 1 1 122 1 0.0 4 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 73 0.0 4 0 0 0 0 73 128 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.11 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 133,492 Reads with adapters: 3,216 (2.4%) Reads written (passing filters): 133,492 (100.0%) Total basepairs processed: 36,571,241 bp Total written (filtered): 36,534,633 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3216 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 2.3% G: 93.9% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 2085.8 0 2 4 3 521.5 0 3 5 3 130.4 0 3 11 3151 0.0 1 12 3139 12 24 0.0 1 0 24 13 20 0.0 1 0 20 14 1 0.0 1 0 1 15 1 0.0 1 0 1 16 5 0.0 1 0 5 152 1 0.0 1 1 210 4 0.0 1 4 265 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.