This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.44 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 154,379 (5.0%) Reads that were too short: 4,239 (0.1%) Reads written (passing filters): 150,140 (4.9%) Total basepairs processed: 931,490,553 bp Total written (filtered): 42,706,229 bp (4.6%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 154379 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 651 755.5 0 651 7 1664 755.5 0 1664 8 2291 755.5 0 2291 9 133992 755.5 0 133992 10 152 755.5 0 152 11 11 755.5 0 11 12 8 755.5 0 8 13 6 755.5 0 6 14 11 755.5 0 11 15 10 755.5 0 10 16 35 755.5 0 35 17 37 755.5 0 37 18 124 755.5 0 124 19 11 755.5 0 11 20 9 755.5 0 9 21 8 755.5 0 8 22 8 755.5 0 8 23 7 755.5 0 7 24 11 755.5 0 11 25 6 755.5 0 6 26 4 755.5 0 4 27 14 755.5 0 14 28 8 755.5 0 8 29 6 755.5 0 6 30 7 755.5 0 7 31 2 755.5 0 2 32 12 755.5 0 12 33 7 755.5 0 7 34 5 755.5 0 5 35 9 755.5 0 9 36 3 755.5 0 3 37 13 755.5 0 13 38 9 755.5 0 9 39 12 755.5 0 12 40 15 755.5 0 15 41 13 755.5 0 13 42 10 755.5 0 10 43 12 755.5 0 12 44 12 755.5 0 12 45 9 755.5 0 9 46 30 755.5 0 30 47 81 755.5 0 81 48 142 755.5 0 142 49 6 755.5 0 6 50 5 755.5 0 5 51 13 755.5 0 13 52 10 755.5 0 10 53 7 755.5 0 7 54 6 755.5 0 6 55 6 755.5 0 6 56 48 755.5 0 48 57 46 755.5 0 46 58 77 755.5 0 77 59 10 755.5 0 10 60 6 755.5 0 6 61 8 755.5 0 8 62 5 755.5 0 5 63 14 755.5 0 14 64 8 755.5 0 8 65 2 755.5 0 2 66 13 755.5 0 13 67 12 755.5 0 12 68 10 755.5 0 10 69 6 755.5 0 6 70 3 755.5 0 3 71 10 755.5 0 10 72 10 755.5 0 10 73 12 755.5 0 12 74 12 755.5 0 12 75 5 755.5 0 5 76 12 755.5 0 12 77 11 755.5 0 11 78 31 755.5 0 31 79 134 755.5 0 134 80 54 755.5 0 54 81 264 755.5 0 264 82 103 755.5 0 103 83 81 755.5 0 81 84 61 755.5 0 61 85 19 755.5 0 19 86 21 755.5 0 21 87 32 755.5 0 32 88 22 755.5 0 22 89 39 755.5 0 39 90 26 755.5 0 26 91 34 755.5 0 34 92 36 755.5 0 36 93 63 755.5 0 63 94 878 755.5 0 878 95 1865 755.5 0 1865 96 2278 755.5 0 2278 97 144 755.5 0 144 98 164 755.5 0 164 99 15 755.5 0 15 100 24 755.5 0 24 101 55 755.5 0 55 102 16 755.5 0 16 103 18 755.5 0 18 104 5 755.5 0 5 105 6 755.5 0 6 106 13 755.5 0 13 107 15 755.5 0 15 108 6 755.5 0 6 109 12 755.5 0 12 110 156 755.5 0 156 111 22 755.5 0 22 112 28 755.5 0 28 113 30 755.5 0 30 114 6 755.5 0 6 115 15 755.5 0 15 116 22 755.5 0 22 117 15 755.5 0 15 118 56 755.5 0 56 119 25 755.5 0 25 120 23 755.5 0 23 121 16 755.5 0 16 122 14 755.5 0 14 123 22 755.5 0 22 124 15 755.5 0 15 125 19 755.5 0 19 126 35 755.5 0 35 127 16 755.5 0 16 128 19 755.5 0 19 129 11 755.5 0 11 130 28 755.5 0 28 131 56 755.5 0 56 132 71 755.5 0 71 133 81 755.5 0 81 134 23 755.5 0 23 135 27 755.5 0 27 136 53 755.5 0 53 137 115 755.5 0 115 138 77 755.5 0 77 139 109 755.5 0 109 140 23 755.5 0 23 141 35 755.5 0 35 142 19 755.5 0 19 143 40 755.5 0 40 144 29 755.5 0 29 145 248 755.5 0 248 146 268 755.5 0 268 147 30 755.5 0 30 148 9 755.5 0 9 149 12 755.5 0 12 150 16 755.5 0 16 151 21 755.5 0 21 152 11 755.5 0 11 153 4 755.5 0 4 154 9 755.5 0 9 155 9 755.5 0 9 156 17 755.5 0 17 157 33 755.5 0 33 158 53 755.5 0 53 159 26 755.5 0 26 160 30 755.5 0 30 161 13 755.5 0 13 162 21 755.5 0 21 163 20 755.5 0 20 164 16 755.5 0 16 165 38 755.5 0 38 166 26 755.5 0 26 167 24 755.5 0 24 168 17 755.5 0 17 169 13 755.5 0 13 170 31 755.5 0 31 171 20 755.5 0 20 172 20 755.5 0 20 173 11 755.5 0 11 174 22 755.5 0 22 175 15 755.5 0 15 176 18 755.5 0 18 177 12 755.5 0 12 178 8 755.5 0 8 179 38 755.5 0 38 180 44 755.5 0 44 181 46 755.5 0 46 182 27 755.5 0 27 183 28 755.5 0 28 184 32 755.5 0 32 185 177 755.5 0 177 186 52 755.5 0 52 187 76 755.5 0 76 188 38 755.5 0 38 189 9 755.5 0 9 190 13 755.5 0 13 191 31 755.5 0 31 192 119 755.5 0 119 193 71 755.5 0 71 194 319 755.5 0 319 195 32 755.5 0 32 196 8 755.5 0 8 197 12 755.5 0 12 198 14 755.5 0 14 199 21 755.5 0 21 200 16 755.5 0 16 201 11 755.5 0 11 202 5 755.5 0 5 203 33 755.5 0 33 204 77 755.5 0 77 205 349 755.5 0 349 206 103 755.5 0 103 207 52 755.5 0 52 208 14 755.5 0 14 209 14 755.5 0 14 210 16 755.5 0 16 211 34 755.5 0 34 212 58 755.5 0 58 213 45 755.5 0 45 214 32 755.5 0 32 215 31 755.5 0 31 216 22 755.5 0 22 217 23 755.5 0 23 218 32 755.5 0 32 219 35 755.5 0 35 220 50 755.5 0 50 221 7 755.5 0 7 222 5 755.5 0 5 223 30 755.5 0 30 224 34 755.5 0 34 225 38 755.5 0 38 226 23 755.5 0 23 227 50 755.5 0 50 228 49 755.5 0 49 229 39 755.5 0 39 230 16 755.5 0 16 231 35 755.5 0 35 232 24 755.5 0 24 233 22 755.5 0 22 234 22 755.5 0 22 235 50 755.5 0 50 236 41 755.5 0 41 237 41 755.5 0 41 238 54 755.5 0 54 239 51 755.5 0 51 240 79 755.5 0 79 241 13 755.5 0 13 242 7 755.5 0 7 243 14 755.5 0 14 244 25 755.5 0 25 245 27 755.5 0 27 246 20 755.5 0 20 247 35 755.5 0 35 248 24 755.5 0 24 249 17 755.5 0 17 250 6 755.5 0 6 251 7 755.5 0 7 252 22 755.5 0 22 253 44 755.5 0 44 254 66 755.5 0 66 255 29 755.5 0 29 256 24 755.5 0 24 257 40 755.5 0 40 258 20 755.5 0 20 259 26 755.5 0 26 260 9 755.5 0 9 261 63 755.5 0 63 262 109 755.5 0 109 263 130 755.5 0 130 264 37 755.5 0 37 265 27 755.5 0 27 266 40 755.5 0 40 267 58 755.5 0 58 268 38 755.5 0 38 269 20 755.5 0 20 270 6 755.5 0 6 271 84 755.5 0 84 272 217 755.5 0 217 273 51 755.5 0 51 274 64 755.5 0 64 275 31 755.5 0 31 276 24 755.5 0 24 277 13 755.5 0 13 278 20 755.5 0 20 279 69 755.5 0 69 280 49 755.5 0 49 281 20 755.5 0 20 282 22 755.5 0 22 283 17 755.5 0 17 284 33 755.5 0 33 285 22 755.5 0 22 286 36 755.5 0 36 287 54 755.5 0 54 288 57 755.5 0 57 289 29 755.5 0 29 290 30 755.5 0 30 291 29 755.5 0 29 292 79 755.5 0 79 293 49 755.5 0 49 294 33 755.5 0 33 295 29 755.5 0 29 296 57 755.5 0 57 297 33 755.5 0 33 298 77 755.5 0 77 299 109 755.5 0 109 300 34 755.5 0 34 301 30 755.5 0 30 Finished in 6.56 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 150,140 Reads with adapters: 138,247 (92.1%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 138,247 (92.1%) Total basepairs processed: 42,706,229 bp Total written (filtered): 38,023,707 bp (89.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 138247 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 52 0.0 3 0 0 52 16 903 0.0 3 0 891 10 2 17 136913 0.0 3 134220 2639 49 5 18 218 0.0 3 5 205 5 3 19 75 0.0 3 0 1 63 11 20 62 0.0 3 42 1 0 19 21 1 0.0 3 1 22 3 0.0 3 0 0 1 2 26 1 0.0 3 1 27 1 0.0 3 0 1 28 1 0.0 3 1 29 1 0.0 3 1 30 1 0.0 3 1 31 1 0.0 3 1 32 2 0.0 3 2 35 1 0.0 3 1 40 2 0.0 3 2 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.01 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 138,247 Reads with adapters: 23,388 (16.9%) Reads written (passing filters): 138,247 (100.0%) Total basepairs processed: 38,023,707 bp Total written (filtered): 37,953,463 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 23388 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.5% C: 0.8% G: 55.3% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 23326 2160.1 0 23326 4 60 540.0 0 60 13 2 0.0 1 0 2