This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.11 s (29 us/read; 2.06 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 38,770 (1.3%) Reads that were too short: 13,017 (0.4%) Reads written (passing filters): 25,753 (0.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 4,750,888 bp (0.5%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 38770 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1938 755.5 0 1938 7 46 755.5 0 46 8 50 755.5 0 50 9 2218 755.5 0 2218 10 6 755.5 0 6 11 7 755.5 0 7 12 16 755.5 0 16 13 9 755.5 0 9 14 12 755.5 0 12 15 6 755.5 0 6 16 19 755.5 0 19 17 22 755.5 0 22 18 13 755.5 0 13 19 4 755.5 0 4 20 3 755.5 0 3 21 10 755.5 0 10 22 4 755.5 0 4 23 6 755.5 0 6 24 9 755.5 0 9 25 5 755.5 0 5 26 15 755.5 0 15 27 15 755.5 0 15 28 14 755.5 0 14 29 7 755.5 0 7 30 16 755.5 0 16 31 11 755.5 0 11 32 11 755.5 0 11 33 17 755.5 0 17 34 7 755.5 0 7 35 11 755.5 0 11 36 4 755.5 0 4 37 10 755.5 0 10 38 12 755.5 0 12 39 11 755.5 0 11 40 18 755.5 0 18 41 14 755.5 0 14 42 15 755.5 0 15 43 24 755.5 0 24 44 204 755.5 0 204 45 88 755.5 0 88 46 36 755.5 0 36 47 9 755.5 0 9 48 12 755.5 0 12 49 12 755.5 0 12 50 10 755.5 0 10 51 24 755.5 0 24 52 17 755.5 0 17 53 132 755.5 0 132 54 22 755.5 0 22 55 7 755.5 0 7 56 24 755.5 0 24 57 34 755.5 0 34 58 57 755.5 0 57 59 60 755.5 0 60 60 75 755.5 0 75 61 15 755.5 0 15 62 4 755.5 0 4 63 7 755.5 0 7 64 9 755.5 0 9 65 10 755.5 0 10 66 6 755.5 0 6 67 8 755.5 0 8 68 9 755.5 0 9 69 12 755.5 0 12 70 4 755.5 0 4 71 4 755.5 0 4 72 3 755.5 0 3 73 13 755.5 0 13 74 45 755.5 0 45 75 92 755.5 0 92 76 12 755.5 0 12 77 4 755.5 0 4 78 9 755.5 0 9 79 17 755.5 0 17 80 18 755.5 0 18 81 48 755.5 0 48 82 61 755.5 0 61 83 41 755.5 0 41 84 29 755.5 0 29 85 21 755.5 0 21 86 18 755.5 0 18 87 19 755.5 0 19 88 18 755.5 0 18 89 13 755.5 0 13 90 36 755.5 0 36 91 75 755.5 0 75 92 47 755.5 0 47 93 72 755.5 0 72 94 50 755.5 0 50 95 27 755.5 0 27 96 44 755.5 0 44 97 103 755.5 0 103 98 39 755.5 0 39 99 23 755.5 0 23 100 16 755.5 0 16 101 12 755.5 0 12 102 12 755.5 0 12 103 17 755.5 0 17 104 70 755.5 0 70 105 33 755.5 0 33 106 385 755.5 0 385 107 350 755.5 0 350 108 413 755.5 0 413 109 16 755.5 0 16 110 18 755.5 0 18 111 19 755.5 0 19 112 35 755.5 0 35 113 52 755.5 0 52 114 282 755.5 0 282 115 373 755.5 0 373 116 357 755.5 0 357 117 30 755.5 0 30 118 17 755.5 0 17 119 74 755.5 0 74 120 50 755.5 0 50 121 65 755.5 0 65 122 37 755.5 0 37 123 55 755.5 0 55 124 55 755.5 0 55 125 69 755.5 0 69 126 37 755.5 0 37 127 46 755.5 0 46 128 126 755.5 0 126 129 87 755.5 0 87 130 73 755.5 0 73 131 64 755.5 0 64 132 62 755.5 0 62 133 88 755.5 0 88 134 255 755.5 0 255 135 169 755.5 0 169 136 92 755.5 0 92 137 95 755.5 0 95 138 322 755.5 0 322 139 130 755.5 0 130 140 78 755.5 0 78 141 96 755.5 0 96 142 349 755.5 0 349 143 255 755.5 0 255 144 87 755.5 0 87 145 83 755.5 0 83 146 127 755.5 0 127 147 223 755.5 0 223 148 6575 755.5 0 6575 149 1074 755.5 0 1074 150 941 755.5 0 941 151 45 755.5 0 45 152 32 755.5 0 32 153 61 755.5 0 61 154 70 755.5 0 70 155 440 755.5 0 440 156 98 755.5 0 98 157 109 755.5 0 109 158 116 755.5 0 116 159 323 755.5 0 323 160 74 755.5 0 74 161 68 755.5 0 68 162 155 755.5 0 155 163 460 755.5 0 460 164 142 755.5 0 142 165 211 755.5 0 211 166 105 755.5 0 105 167 93 755.5 0 93 168 77 755.5 0 77 169 30 755.5 0 30 170 37 755.5 0 37 171 55 755.5 0 55 172 53 755.5 0 53 173 25 755.5 0 25 174 29 755.5 0 29 175 55 755.5 0 55 176 36 755.5 0 36 177 34 755.5 0 34 178 29 755.5 0 29 179 35 755.5 0 35 180 124 755.5 0 124 181 201 755.5 0 201 182 184 755.5 0 184 183 119 755.5 0 119 184 117 755.5 0 117 185 95 755.5 0 95 186 52 755.5 0 52 187 41 755.5 0 41 188 33 755.5 0 33 189 53 755.5 0 53 190 32 755.5 0 32 191 63 755.5 0 63 192 82 755.5 0 82 193 52 755.5 0 52 194 44 755.5 0 44 195 41 755.5 0 41 196 59 755.5 0 59 197 19 755.5 0 19 198 18 755.5 0 18 199 28 755.5 0 28 200 115 755.5 0 115 201 95 755.5 0 95 202 154 755.5 0 154 203 54 755.5 0 54 204 132 755.5 0 132 205 77 755.5 0 77 206 52 755.5 0 52 207 84 755.5 0 84 208 72 755.5 0 72 209 51 755.5 0 51 210 97 755.5 0 97 211 38 755.5 0 38 212 80 755.5 0 80 213 70 755.5 0 70 214 84 755.5 0 84 215 125 755.5 0 125 216 102 755.5 0 102 217 70 755.5 0 70 218 71 755.5 0 71 219 58 755.5 0 58 220 80 755.5 0 80 221 93 755.5 0 93 222 27 755.5 0 27 223 23 755.5 0 23 224 79 755.5 0 79 225 96 755.5 0 96 226 130 755.5 0 130 227 119 755.5 0 119 228 111 755.5 0 111 229 97 755.5 0 97 230 70 755.5 0 70 231 99 755.5 0 99 232 175 755.5 0 175 233 101 755.5 0 101 234 58 755.5 0 58 235 68 755.5 0 68 236 80 755.5 0 80 237 65 755.5 0 65 238 24 755.5 0 24 239 29 755.5 0 29 240 51 755.5 0 51 241 35 755.5 0 35 242 35 755.5 0 35 243 37 755.5 0 37 244 76 755.5 0 76 245 73 755.5 0 73 246 102 755.5 0 102 247 83 755.5 0 83 248 134 755.5 0 134 249 69 755.5 0 69 250 72 755.5 0 72 251 30 755.5 0 30 252 41 755.5 0 41 253 59 755.5 0 59 254 169 755.5 0 169 255 255 755.5 0 255 256 156 755.5 0 156 257 118 755.5 0 118 258 154 755.5 0 154 259 82 755.5 0 82 260 56 755.5 0 56 261 45 755.5 0 45 262 33 755.5 0 33 263 52 755.5 0 52 264 31 755.5 0 31 265 82 755.5 0 82 266 129 755.5 0 129 267 93 755.5 0 93 268 208 755.5 0 208 269 606 755.5 0 606 270 144 755.5 0 144 271 114 755.5 0 114 272 144 755.5 0 144 273 241 755.5 0 241 274 53 755.5 0 53 275 119 755.5 0 119 276 93 755.5 0 93 277 66 755.5 0 66 278 78 755.5 0 78 279 76 755.5 0 76 280 138 755.5 0 138 281 128 755.5 0 128 282 124 755.5 0 124 283 65 755.5 0 65 284 53 755.5 0 53 285 55 755.5 0 55 286 96 755.5 0 96 287 99 755.5 0 99 288 98 755.5 0 98 289 106 755.5 0 106 290 99 755.5 0 99 291 101 755.5 0 101 292 98 755.5 0 98 293 178 755.5 0 178 294 667 755.5 0 667 295 2123 755.5 0 2123 296 797 755.5 0 797 297 319 755.5 0 319 298 185 755.5 0 185 299 60 755.5 0 60 300 36 755.5 0 36 301 3 755.5 0 3 Finished in 1.15 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 25,753 Reads with adapters: 2,284 (8.9%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 2,281 (8.9%) Total basepairs processed: 4,750,888 bp Total written (filtered): 619,928 bp (13.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2284 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 2 1 19 19 0.0 3 0 19 20 2230 0.0 4 2152 65 8 4 1 21 12 0.0 4 5 6 0 1 22 10 0.0 4 7 1 1 1 23 2 0.0 4 0 0 0 2 36 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 2,281 Reads with adapters: 126 (5.5%) Reads written (passing filters): 2,281 (100.0%) Total basepairs processed: 619,928 bp Total written (filtered): 617,779 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 126 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.3% C: 3.2% G: 88.9% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 5 1 2.2 0 1 9 35 0.0 0 2 33 10 51 0.0 1 2 49 11 35 0.0 1 0 35 146 1 0.0 1 1 258 1 0.0 1 1 263 1 0.0 1 1 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.